##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139694_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13559157
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.365886242042922	31.0	25.0	33.0	18.0	33.0
2	30.991169362520104	33.0	31.0	33.0	27.0	33.0
3	31.102627250351922	33.0	31.0	33.0	27.0	34.0
4	31.876914471895265	33.0	32.0	33.0	29.0	34.0
5	32.333481130132206	33.0	33.0	34.0	31.0	34.0
6	35.91029206314227	38.0	36.0	38.0	31.0	38.0
7	36.223723790498184	38.0	37.0	38.0	33.0	38.0
8	36.47885926831587	38.0	37.0	38.0	34.0	38.0
9	36.68426348334192	38.0	38.0	38.0	34.0	38.0
10-11	36.7146003988301	38.0	38.0	38.0	34.0	38.0
12-13	36.76250485188717	38.0	38.0	38.0	34.0	38.0
14-15	36.77294668835238	38.0	38.0	38.0	34.0	38.0
16-17	36.76382112103282	38.0	38.0	38.0	34.0	38.0
18-19	36.768660507434205	38.0	38.0	38.0	34.5	38.0
20-21	36.79702297536945	38.0	38.0	38.0	35.0	38.0
22-23	36.77539120253928	38.0	38.0	38.0	34.5	38.0
24-25	36.78374861315018	38.0	38.0	38.0	35.0	38.0
26-27	36.7511800180564	38.0	38.0	38.0	35.0	38.0
28-29	36.67344509579061	38.0	38.0	38.0	34.0	38.0
30-31	36.69859367619186	38.0	38.0	38.0	34.0	38.0
32-33	36.69598451837556	38.0	38.0	38.0	34.0	38.0
34-35	36.683170620710385	38.0	38.0	38.0	34.0	38.0
36-37	36.65905154299165	38.0	38.0	38.0	34.0	38.0
38-39	36.647533486346816	38.0	38.0	38.0	34.0	38.0
40-41	36.64392344579547	38.0	38.0	38.0	34.0	38.0
42-43	36.62087979382855	38.0	38.0	38.0	34.0	38.0
44-45	36.561508896589224	38.0	38.0	38.0	34.0	38.0
46-47	36.444077219587	38.0	38.0	38.0	34.0	38.0
48-49	36.39702091307883	38.0	38.0	38.0	34.0	38.0
50-51	36.4089300018451	38.0	38.0	38.0	34.0	38.0
52-53	36.411023390686616	38.0	38.0	38.0	34.0	38.0
54-55	36.33924149241865	38.0	38.0	38.0	33.5	38.0
56-57	36.3583470781961	38.0	38.0	38.0	34.0	38.0
58-59	36.303526666409425	38.0	38.0	38.0	33.5	38.0
60-61	36.31736251460805	38.0	38.0	38.0	33.0	38.0
62-63	36.33439590474674	38.0	38.0	38.0	33.0	38.0
64-65	36.32315785875929	38.0	38.0	38.0	33.5	38.0
66-67	36.30841482994915	38.0	38.0	38.0	33.0	38.0
68-69	36.290890823420824	38.0	38.0	38.0	33.0	38.0
70-71	36.291688026799164	38.0	38.0	38.0	33.0	38.0
72-73	36.28470365425798	38.0	38.0	38.0	33.0	38.0
74-75	36.2496277040603	38.0	37.0	38.0	33.0	38.0
76-77	36.239881076387746	38.0	37.0	38.0	33.0	38.0
78-79	36.22948049106806	38.0	37.0	38.0	33.0	38.0
80-81	36.2373404079429	38.0	37.0	38.0	33.0	38.0
82-83	36.20673603422894	38.0	37.0	38.0	33.0	38.0
84-85	36.17580048893865	38.0	37.0	38.0	33.0	38.0
86-87	36.17614096375989	38.0	37.0	38.0	33.0	38.0
88-89	36.181745925865485	38.0	37.0	38.0	33.0	38.0
90-91	36.12950965941845	38.0	37.0	38.0	33.0	38.0
92-93	36.149804862445535	38.0	37.0	38.0	33.0	38.0
94-95	36.10378785962405	38.0	37.0	38.0	33.0	38.0
96-97	36.131436376010015	38.0	37.0	38.0	33.0	38.0
98-99	36.17927996977623	38.0	37.0	38.0	33.0	38.0
100-101	35.652608327983344	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	16.0
19	52.0
20	154.0
21	698.0
22	2857.0
23	8703.0
24	20194.0
25	37963.0
26	63102.0
27	96159.0
28	137390.0
29	184631.0
30	237583.0
31	301519.0
32	381718.0
33	496322.0
34	681924.0
35	1059639.0
36	2222105.0
37	7626424.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.95700470169348	20.541785894211564	15.75608277122243	23.74512663287253
2	14.46783159159526	21.91316171056947	41.3156953636572	22.303311334178076
3	17.95616054891908	26.400335950088934	31.131013528348404	24.512489972643582
4	12.758182533029155	18.878961280557487	38.03029200119152	30.332564185221838
5	13.593217692409073	37.33085149732962	35.40686038152325	13.669070428738056
6	29.619909261320597	38.319218517788386	18.61122339685277	13.449648824038249
7	26.15089090596644	32.84347211046511	23.003465679155845	18.002171304412602
8	24.024222154813902	37.25854785810061	20.934811802828154	17.782418184257327
9	25.06648459045057	17.301761459064156	21.39202311766137	36.239730832823895
10-11	23.992594082360725	27.13080171577038	28.723216347446968	20.153387854421922
12-13	24.95826897183973	24.548342999680177	28.98488036367752	21.508507664802575
14-15	22.003524997903632	25.96911813912915	26.79087645345503	25.236480409512186
16-17	21.458804555474945	29.097443152254964	27.461559741509006	21.982192550761084
18-19	21.44978850823838	28.028210013351124	29.47773965593879	21.044261822471707
20-21	22.211465840216615	27.08419293325615	29.533098080294344	21.17124314623289
22-23	21.971496218058174	27.065050874585573	29.336513532750395	21.626939374605854
24-25	21.7428781222093	27.193998370928224	29.435665016694223	21.62745849016825
26-27	21.721083377432226	27.356458798416018	29.361447429847715	21.561010394304038
28-29	21.619654831555156	27.40668025003956	29.376102012882257	21.597562905523027
30-31	21.99356154804219	27.186100344562398	29.31848657956353	21.50185152783188
32-33	21.620176996234765	27.407977713033087	29.306120623736554	21.66572466699559
34-35	21.8128953426309	27.38530284887955	29.318253184083332	21.48354862440622
36-37	21.754160644644227	27.34044015814306	29.314072548929747	21.591326648282966
38-39	21.60421036729208	27.385866805589444	29.414105377882144	21.595817449236336
40-41	21.895586636161983	27.248333539313585	29.244044676023723	21.612035148500706
42-43	21.82220233329588	27.345553487355133	29.091205210119536	21.74103896922945
44-45	21.85864985961729	27.47000436873667	28.91512892552977	21.75621684611627
46-47	21.971866099119342	27.447941349808115	28.567987238364168	22.01220531270838
48-49	21.898074424864227	27.564557263110697	28.56830635883793	21.969061953187147
50-51	21.870985134400488	27.686030654811805	28.495917924937398	21.947066285850312
52-53	22.028123157502574	27.606318634665516	28.42367958969999	21.94187861813192
54-55	21.962234834488914	27.597345943246708	28.36765508966855	22.07276413259582
56-57	22.00684943986016	27.7050644262946	28.303906832592762	21.98417930125247
58-59	21.965504555852025	27.797946919620685	28.371688020193396	21.864860504333894
60-61	22.04409491407691	27.841853992894006	28.305051910139557	21.80899918288953
62-63	21.960126249995938	27.919021081662954	28.26175817134049	21.85909449700062
64-65	21.940375179793506	27.883734798032222	28.24173553282018	21.934154489354096
66-67	21.99015730932088	27.866268582873804	28.135888982857804	22.00768512494751
68-69	21.947596949546337	27.81483559387513	28.24897214755794	21.988595309020596
70-71	22.00914173226163	27.537225349651955	28.296573205001224	22.157059713085197
72-73	22.07736484967235	27.547287689340717	28.249954470183695	22.125392990803235
74-75	21.970718699097112	27.66996065114154	28.21675625075479	22.14256439900656
76-77	22.069697326830067	27.604050823944533	28.1760348984454	22.15021695078
78-79	21.9860713046162	27.65827020053261	28.14078190832233	22.214876586528863
80-81	22.03004423586855	27.695703223231394	28.115483090944398	22.158769449955656
82-83	22.09845874136685	27.65471069220913	28.177406460714984	22.069424105709036
84-85	22.19764273394215	27.63587734232731	28.069341913875647	22.0971380098549
86-87	22.036157742516433	27.68846695049009	28.166228074908204	22.10914723208527
88-89	22.10833594713792	27.694447253734168	28.046637420018744	22.15057937910917
90-91	22.15806287059299	27.701669320301303	28.068435496695933	22.07183231240977
92-93	22.171023536142286	27.701001198624116	28.039200432965185	22.088774832268417
94-95	22.077998416347775	27.769184110493235	28.0360450858199	22.116772387339097
96-97	22.074872384612586	27.780586747387787	28.071906321178393	22.072634546821234
98-99	22.353009688178304	28.341055136650457	28.11210858423522	21.19382659093602
100-101	22.94803443523506	30.85323517620544	25.46136857247116	20.737361816088345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3979.0
1	3754.5
2	4324.0
3	5518.5
4	7030.0
5	10100.5
6	13093.0
7	17365.5
8	22035.5
9	23549.0
10	22092.5
11	20360.0
12	21471.0
13	26658.0
14	38634.0
15	53181.5
16	63224.5
17	67423.5
18	66797.0
19	62688.5
20	57110.0
21	52345.5
22	51614.0
23	57649.0
24	70554.0
25	90112.5
26	115383.0
27	142269.5
28	169836.5
29	198757.0
30	227030.5
31	256961.5
32	289471.0
33	322750.5
34	354849.0
35	383806.5
36	415895.5
37	445288.5
38	466846.5
39	485101.0
40	505182.0
41	525094.5
42	536508.5
43	546690.5
44	564421.5
45	575444.0
46	572288.5
47	567620.0
48	561623.0
49	551896.5
50	539309.0
51	521299.5
52	496494.0
53	468532.0
54	439559.0
55	406477.5
56	370923.0
57	333969.0
58	294364.0
59	254802.5
60	213504.5
61	172054.0
62	134891.5
63	103835.5
64	78525.5
65	58000.0
66	42599.0
67	32050.5
68	24030.0
69	17507.0
70	12679.5
71	9013.5
72	6295.0
73	4319.0
74	2938.5
75	1924.5
76	1222.5
77	742.0
78	450.5
79	292.0
80	189.5
81	124.0
82	82.5
83	55.0
84	39.5
85	31.5
86	23.5
87	18.0
88	13.5
89	8.5
90	5.0
91	2.0
92	2.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.05825583404632014
6	0.0
7	0.0035916687150978487
8	0.0
9	0.0
10-11	0.0
12-13	1.1062634646091936E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.640191240458922E-5
28-29	3.321538059603967E-5
30-31	3.694483094895089E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	8.022499673369657E-4
54-55	1.074915477803513E-4
56-57	0.0030298169705626596
58-59	0.0074128999430477256
60-61	0.0032518519337563895
62-63	0.0058613985321577305
64-65	0.006597643674991199
66-67	0.004916249904656132
68-69	0.003839488757499622
70-71	0.005337049842670085
72-73	0.003299402894222435
74-75	0.0028680687090183944
76-77	0.002543860653278328
78-79	1.0187223901317869E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	5.533695919535634E-5
88-89	1.3981716482201998E-5
90-91	2.2621508605787409E-4
92-93	0.0
94-95	1.0126259996065224E-4
96-97	4.886108476005665E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	771.0
22-23	1684.0
24-25	1993.0
26-27	4471.0
28-29	10373.0
30-31	25565.0
32-33	33145.0
34-35	45188.0
36-37	70409.0
38-39	83443.0
40-41	83129.0
42-43	95402.0
44-45	113971.0
46-47	114315.0
48-49	105403.0
50-51	95344.0
52-53	91195.0
54-55	98163.0
56-57	102578.0
58-59	102302.0
60-61	100204.0
62-63	97337.0
64-65	105565.0
66-67	111703.0
68-69	113343.0
70-71	118281.0
72-73	102059.0
74-75	104676.0
76-77	110022.0
78-79	113837.0
80-81	114417.0
82-83	109519.0
84-85	108727.0
86-87	112967.0
88-89	118002.0
90-91	120461.0
92-93	118795.0
94-95	128405.0
96-97	203414.0
98-99	1123296.0
100-101	8945129.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.03326426732825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.39402379904209	75.12071625824736
2	7.2295022551841335	12.150373470622677
3	1.8597357280619797	4.688389917108733
4	0.6769034071904433	2.275296115995577
5	0.3039174498418894	1.2769587689007986
6	0.17087851488627254	0.8615687639428036
7	0.10827428137763001	0.6369048910253009
8	0.06397660747715399	0.4300930530443847
9	0.04451340212450033	0.3366545835749388
>10	0.1443276916915685	1.8751858174481935
>50	0.003220401665911865	0.18508858217093124
>100	6.654945489983764E-4	0.1069790260863587
>500	1.524454054696776E-5	0.008198710428270993
>1k	4.572236685704265E-5	0.04759204140366914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.375089764061291E-6
2	0.0	1.4750179528122582E-5	0.0	0.0	7.375089764061291E-6
3	0.0	1.4750179528122582E-5	0.0	0.0	7.375089764061291E-6
4	0.0	5.900071811249033E-5	0.0	0.0	7.375089764061291E-6
5	0.0	6.637580787655161E-5	0.0	0.0	7.375089764061291E-6
6	6.637580787655161E-5	6.637580787655161E-5	0.0	0.0	1.4750179528122582E-5
7	6.637580787655161E-5	8.11259874046742E-5	0.0	0.0	3.6875448820306456E-5
8	6.637580787655161E-5	8.11259874046742E-5	0.0	0.0	5.1625628348429035E-5
9	6.637580787655161E-5	1.3275161575310323E-4	0.0	1.4750179528122582E-5	5.900071811249033E-5
10-11	7.006335275858226E-5	1.401267055171645E-4	0.0	5.1625628348429035E-5	5.900071811249033E-5
12-13	7.375089764061291E-5	1.4750179528122583E-4	0.0	5.1625628348429035E-5	5.900071811249033E-5
14-15	7.375089764061291E-5	2.5444059686011454E-4	0.0	5.900071811249033E-5	7.375089764061291E-5
16-17	9.218862205076614E-5	2.581281417421452E-4	0.0	7.743844252264356E-5	9.587616693279678E-5
18-19	9.587616693279678E-5	2.8762850079839034E-4	0.0	1.0693880157888871E-4	1.1062634646091937E-4
20-21	9.587616693279678E-5	3.097537700905742E-4	0.0	1.5856442992731774E-4	1.1062634646091937E-4
22-23	9.587616693279678E-5	3.4294167402885E-4	0.0	1.8806478898356294E-4	1.4381425039919517E-4
24-25	9.956371181482743E-5	4.019423921413403E-4	0.0	2.2862778268590002E-4	1.9175233386559357E-4
26-27	1.0325125669685807E-4	4.2775520631555485E-4	0.0	2.91316045680421E-4	1.9175233386559357E-4
28-29	1.1062634646091937E-4	4.3513029607961616E-4	0.0	6.563829890014549E-4	1.954398787476242E-4
30-31	1.1062634646091937E-4	4.5725556537180005E-4	0.0	0.0017773966331387711	2.028149685116855E-4
32-33	1.1431389134295001E-4	4.646306551358613E-4	0.0	0.004137425357638384	2.2862778268590002E-4
34-35	1.216889811070113E-4	5.457566425405355E-4	0.0	0.007308713956184739	2.581281417421452E-4
36-37	1.3275161575310323E-4	6.121324504170871E-4	0.0	0.012205773559521436	2.618156866241758E-4
38-39	1.3275161575310323E-4	6.45320354355363E-4	0.0	0.01885072943694066	2.7287832127026777E-4
40-41	1.3275161575310323E-4	7.301338866420678E-4	0.0	0.026137318123833214	2.8762850079839034E-4
42-43	1.3275161575310323E-4	7.48571611052221E-4	0.0	0.033353843457967186	2.9500359056245165E-4
44-45	1.3275161575310323E-4	8.407602331029872E-4	0.0	0.04136319094173775	2.9869113544448225E-4
46-47	1.3275161575310323E-4	9.108235858615694E-4	0.0	0.050305487280662066	3.318790393827581E-4
48-49	1.3275161575310323E-4	9.145111307436001E-4	0.0	0.05910396936918719	3.5400430867494194E-4
50-51	1.5118934016325648E-4	9.698243039740597E-4	0.0	0.06798357744511697	3.5400430867494194E-4
52-53	1.548768850452871E-4	9.808869386201517E-4	0.0	0.0768299976171085	3.871922126132178E-4
54-55	1.548768850452871E-4	9.956371181482743E-4	0.0	0.08597142137966246	4.2406766143352425E-4
56-57	1.622519748093484E-4	0.0010251374772045194	0.0	0.09274544132795276	4.314427511975855E-4
58-59	1.6962706457340968E-4	0.001039887656732642	0.0	0.09885939074235958	4.461929307257081E-4
60-61	1.8437724410153228E-4	0.0010730755606709178	0.0	0.10461564830320941	4.4988047560773873E-4
62-63	2.2862778268590002E-4	0.0010988883748451323	0.0	0.1097634609585242	4.646306551358613E-4
64-65	2.3231532756793065E-4	0.0012242649008341743	0.0	0.11495921169730537	4.6831820001789196E-4
66-67	2.433779622140226E-4	0.0012242649008341743	0.0	0.12049052902035134	4.756932897819532E-4
68-69	2.433779622140226E-4	0.0012611403496544807	0.0	0.12632053747884178	4.793808346639839E-4
70-71	2.433779622140226E-4	0.001279578074064634	0.0	0.13151260067274095	4.867559244280452E-4
72-73	2.7287832127026777E-4	0.0012869531638286954	0.0	0.13577540255636839	5.310064630124129E-4
74-75	2.7287832127026777E-4	0.0013385787921771242	0.0	0.13941869689981462	5.310064630124129E-4
76-77	2.765658661522984E-4	0.0014086421449357065	0.0	0.14235398262591104	5.346940078944436E-4
78-79	2.80253411034329E-4	0.0014307674142278904	0.0	0.14478407470316923	5.420690976585048E-4
80-81	2.80253411034329E-4	0.0014307674142278904	0.0	0.1459124634370706	5.678819118327195E-4
82-83	2.80253411034329E-4	0.0014307674142278904	0.0	0.14644346990008303	6.047573606530258E-4
84-85	2.80253411034329E-4	0.0014381425039919518	0.0	0.14685647492687046	6.047573606530258E-4
86-87	2.80253411034329E-4	0.0014492051386380436	0.0	0.1470076642670337	6.158199952991177E-4
88-89	2.80253411034329E-4	0.0015045183118685033	0.0	0.14707404007491026	6.231950850631792E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19585	0.0	52.70326	1
GTATCAA	34955	0.0	36.242073	1
TCAACGC	43665	0.0	28.63737	4
ATCAACG	43840	0.0	28.523056	3
CAACGCA	44920	0.0	27.837282	5
TATCAAC	45295	0.0	27.825842	2
AACGCAG	46120	0.0	27.170958	6
ACGCAGA	52345	0.0	23.92325	7
GTGGTAT	8795	0.0	23.639025	1
CGCAGAG	53345	0.0	23.45802	8
TGGTATC	8345	0.0	23.208035	2
GCAGAGT	61730	0.0	20.250685	9
GAGTACT	38075	0.0	19.863302	12-13
CTTATAC	9370	0.0	19.104113	1
CAGAGTA	60465	0.0	18.827919	10-11
AGAGTAC	56320	0.0	18.809137	10-11
GTACATG	39585	0.0	18.327944	1
TACATGG	39450	0.0	18.040781	2
GTACTTT	42365	0.0	17.740389	14-15
AGTACTT	41545	0.0	17.400122	12-13
>>END_MODULE