FastQCFastQC Report
Fri 27 May 2016
SRR2139693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12328409
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181600.050.6562041
GTATCAA321550.036.201371
TCAACGC399750.029.0444434
ATCAACG401450.028.9000743
CAACGCA410450.028.3081915
TATCAAC416350.028.0204032
AACGCAG423950.027.4311056
ACGCAGA478750.024.3001677
CGCAGAG484100.024.0217848
TGGTATC80100.022.712092
GTGGTAT84500.022.5957641
GCAGAGT560300.020.7242419
GAGTACT339750.019.90319812-13
TACATGG385150.019.5845092
GTACATG389750.019.4634511
CTTATAC90050.019.4401761
AGAGTAC514150.019.0441610-11
ACATGGG386100.018.9028933
CAGAGTA548850.018.8870210-11
GTACTTT374150.017.99942814-15
Produced by FastQC (version 0.11.5)