##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139693_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12328409
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.76597564211246	32.0	27.0	33.0	18.0	33.0
2	31.11330732132589	33.0	31.0	33.0	27.0	33.0
3	31.192192439429938	33.0	31.0	33.0	27.0	34.0
4	31.921772468775167	33.0	32.0	33.0	29.0	34.0
5	32.357535672283426	33.0	33.0	34.0	31.0	34.0
6	35.94260630061835	38.0	36.0	38.0	31.0	38.0
7	36.25150495899349	38.0	37.0	38.0	33.0	38.0
8	36.49956381232972	38.0	37.0	38.0	34.0	38.0
9	36.69188733112278	38.0	38.0	38.0	34.0	38.0
10-11	36.71586061916018	38.0	38.0	38.0	34.0	38.0
12-13	36.764620925538736	38.0	38.0	38.0	34.0	38.0
14-15	36.77563491769295	38.0	38.0	38.0	34.5	38.0
16-17	36.767800816796395	38.0	38.0	38.0	34.5	38.0
18-19	36.772598151148294	38.0	38.0	38.0	34.5	38.0
20-21	36.79966600652401	38.0	38.0	38.0	35.0	38.0
22-23	36.77756843069966	38.0	38.0	38.0	34.5	38.0
24-25	36.784860300551045	38.0	38.0	38.0	35.0	38.0
26-27	36.75248876916385	38.0	38.0	38.0	34.5	38.0
28-29	36.674797475136785	38.0	38.0	38.0	34.0	38.0
30-31	36.70031445604083	38.0	38.0	38.0	34.0	38.0
32-33	36.69496998522996	38.0	38.0	38.0	34.0	38.0
34-35	36.680483158389734	38.0	38.0	38.0	34.0	38.0
36-37	36.65547322716891	38.0	38.0	38.0	34.0	38.0
38-39	36.642655457368576	38.0	38.0	38.0	34.0	38.0
40-41	36.64304776563285	38.0	38.0	38.0	34.0	38.0
42-43	36.62204172792654	38.0	38.0	38.0	34.0	38.0
44-45	36.56179908094285	38.0	38.0	38.0	34.0	38.0
46-47	36.444505480656765	38.0	38.0	38.0	34.0	38.0
48-49	36.395187787749556	38.0	38.0	38.0	34.0	38.0
50-51	36.40709684196247	38.0	38.0	38.0	34.0	38.0
52-53	36.41190992472539	38.0	38.0	38.0	34.0	38.0
54-55	36.33792609226814	38.0	38.0	38.0	33.0	38.0
56-57	36.359407286670034	38.0	38.0	38.0	34.0	38.0
58-59	36.30643927901327	38.0	38.0	38.0	33.5	38.0
60-61	36.31958093600717	38.0	38.0	38.0	33.0	38.0
62-63	36.335985238357765	38.0	38.0	38.0	33.0	38.0
64-65	36.32604966138523	38.0	38.0	38.0	33.5	38.0
66-67	36.30911349776851	38.0	38.0	38.0	33.0	38.0
68-69	36.29026269087092	38.0	38.0	38.0	33.0	38.0
70-71	36.293537481170986	38.0	38.0	38.0	33.0	38.0
72-73	36.28574306487327	38.0	38.0	38.0	33.0	38.0
74-75	36.24754042185093	38.0	37.0	38.0	33.0	38.0
76-77	36.240050441921696	38.0	37.0	38.0	33.0	38.0
78-79	36.22912620252272	38.0	37.0	38.0	33.0	38.0
80-81	36.23703427930697	38.0	37.0	38.0	33.0	38.0
82-83	36.20991155835324	38.0	37.0	38.0	33.0	38.0
84-85	36.175674835802226	38.0	37.0	38.0	33.0	38.0
86-87	36.17564301491452	38.0	37.0	38.0	33.0	38.0
88-89	36.18171046119045	38.0	37.0	38.0	33.0	38.0
90-91	36.12969160716031	38.0	37.0	38.0	33.0	38.0
92-93	36.14887796590111	38.0	37.0	38.0	33.0	38.0
94-95	36.104250474986856	38.0	37.0	38.0	33.0	38.0
96-97	36.13287114778969	38.0	37.0	38.0	33.0	38.0
98-99	36.17941515787858	38.0	37.0	38.0	33.0	38.0
100-101	35.64394160880733	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	10.0
19	53.0
20	129.0
21	606.0
22	2571.0
23	7908.0
24	18163.0
25	34907.0
26	57341.0
27	87865.0
28	124953.0
29	168258.0
30	218032.0
31	274366.0
32	348145.0
33	450332.0
34	617785.0
35	957398.0
36	1990098.0
37	6969480.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.08474248380306	20.586176204893917	15.64148301698946	23.687598294313563
2	14.433906272901881	21.772387661700712	41.40435314889375	22.389352916503665
3	17.972505616904826	26.431829119231846	31.068923816528148	24.526741447335176
4	12.726435341332365	18.721401926233952	37.97742271529116	30.57474001714252
5	13.496273367995059	37.486404507231	35.3813137525232	13.63600837225074
6	29.71822718052706	38.26470848991277	18.4863705424391	13.530693787121079
7	26.15186241862798	32.901209511915525	22.97488600698186	17.97204206247463
8	24.147560321854993	37.2166027262723	20.87965284084913	17.756184111023572
9	25.09834805123678	17.27795533065134	21.355983566087076	36.267713052024796
10-11	24.057033636700403	27.111973653696918	28.665706175062816	20.16528653453986
12-13	24.924902101102678	24.562539808973703	28.94689358702655	21.565664502897068
14-15	22.014531639889622	25.972325382780536	26.747814742356457	25.26532823497338
16-17	21.435312537084066	29.087524594617197	27.45094277777449	22.02622009052425
18-19	21.47279101463944	27.987877430088503	29.455524228633234	21.083807326638823
20-21	22.273898889058916	27.064219643958786	29.459185345021112	21.202696121961186
22-23	21.988482906395447	27.076514762994027	29.289065632036753	21.645936698573777
24-25	21.7456240885694	27.22874431986736	29.409180206651175	21.61645138491206
26-27	21.71759617389066	27.33847027845983	29.34046451112209	21.60346903652742
28-29	21.55568441025668	27.299786144944488	29.443211710416268	21.701317734382563
30-31	21.925292890155255	27.116714787989913	29.398047901505702	21.55994442034913
32-33	21.660364582232543	27.414346240847664	29.24988967127231	21.675399505647484
34-35	21.773119394044404	27.394250708639216	29.337661579807783	21.494968317508594
36-37	21.838871954676055	27.275036514631335	29.234256987476183	21.651834543216424
38-39	21.62236306873404	27.383101902599332	29.359533803829667	21.635001224836962
40-41	21.908779726595352	27.2127161747845	29.233521599781113	21.64498249883903
42-43	21.829574944752363	27.325872345818674	29.046963457558096	21.79758925187086
44-45	21.845029189613623	27.455270947557143	28.87937902677575	21.82032083605348
46-47	22.03855493069881	27.41826323893781	28.531405056481706	22.01177677388167
48-49	21.95351459901312	27.524582019916306	28.490417602118356	22.03148577895222
50-51	21.862635674122977	27.673332862636062	28.464554482653416	21.99947698058755
52-53	22.057601954798596	27.58811086801776	28.404733496919626	21.949553680264014
54-55	21.983452717108836	27.537252754630565	28.355607991977894	22.1236865362827
56-57	22.037350419297088	27.692457086481063	28.25923368122336	22.01095881299849
58-59	21.93067496939838	27.808874653805592	28.330101932050844	21.930348444745185
60-61	22.052940345116447	27.863385134553393	28.235631384113656	21.848043136216504
62-63	21.93600095473002	27.945461138560155	28.22467506476493	21.89386284194489
64-65	21.930774651065306	27.90342252128181	28.206964751686435	21.958838075966447
66-67	22.026196717034633	27.899691314045732	28.064564784778256	22.00954718414138
68-69	21.956460289775134	27.819814299132496	28.197836405950678	22.025889005141693
70-71	21.993477282078505	27.52908822443357	28.27695760772622	22.200476885761706
72-73	22.100090769545698	27.553760743404297	28.16728660985781	22.178861877192187
74-75	21.929252057755914	27.66514313178796	28.199385869461164	22.20621894099496
76-77	22.071136166103145	27.582861068679687	28.149539695358623	22.19646306985855
78-79	22.035129331932445	27.656273092176637	28.078276316791555	22.230321259099366
80-81	22.038653340233143	27.69543606796121	28.058456622136656	22.207453969668993
82-83	22.073836049241052	27.66390758814107	28.13703750573056	22.125218856887322
84-85	22.20680924063933	27.636691278012748	28.024583519436707	22.131915961911215
86-87	22.03317540481743	27.67839334601544	28.104247701178238	22.184183547988894
88-89	22.100624008084697	27.67103409892804	28.0578256173657	22.170516275621566
90-91	22.15163601628337	27.691827041533678	28.055407211785315	22.101129730397634
92-93	22.17395791455061	27.70131886381429	28.00204834713248	22.12267487450262
94-95	22.064381726723568	27.722012956681525	28.024805730145598	22.188799586449313
96-97	22.106503410995735	27.75002012141729	28.02995985034788	22.113516617239092
98-99	22.350360260976526	28.340196424787855	28.05591026720019	21.25353304703543
100-101	22.935216890409986	30.838446108087513	25.43833174962362	20.78800525187888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3772.0
1	3611.5
2	4038.0
3	5023.5
4	6532.0
5	9430.5
6	12112.5
7	15665.5
8	19261.5
9	20580.0
10	19675.5
11	18177.5
12	19336.5
13	23978.0
14	34504.0
15	47851.0
16	57182.0
17	61167.0
18	60677.0
19	57019.0
20	52216.0
21	48014.5
22	47143.5
23	52308.0
24	64244.0
25	82714.0
26	106277.0
27	131899.5
28	157728.0
29	183957.5
30	207739.0
31	232587.0
32	262231.0
33	293330.0
34	324077.0
35	352435.5
36	382646.0
37	409531.0
38	429720.5
39	446022.5
40	462166.5
41	478743.5
42	489796.0
43	500610.0
44	516418.5
45	525166.5
46	520961.0
47	516162.0
48	509372.0
49	499144.5
50	487935.5
51	472683.0
52	452365.5
53	428740.0
54	403037.0
55	373139.5
56	341633.5
57	307536.0
58	273433.0
59	238611.5
60	200107.0
61	162774.5
62	127960.0
63	97688.0
64	73342.5
65	54310.5
66	39313.5
67	29346.5
68	22247.0
69	16184.0
70	11750.0
71	8321.0
72	5681.0
73	3899.0
74	2591.5
75	1644.5
76	1068.0
77	636.0
78	376.0
79	231.5
80	152.0
81	97.0
82	63.5
83	49.5
84	41.5
85	28.0
86	16.5
87	10.5
88	6.5
89	3.5
90	3.5
91	4.0
92	3.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.05864503684133127
6	1.622269345541667E-5
7	0.0037393308414735427
8	0.0
9	0.0
10-11	0.0
12-13	1.2167020091562505E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.085911403168138E-5
28-29	2.0295626089621426E-5
30-31	3.250936320456171E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	8.42856949602085E-6
46-47	0.0
48-49	0.0
50-51	4.328914002746782E-6
52-53	7.460275017148817E-4
54-55	1.538854402917457E-4
56-57	0.0029574396500519746
58-59	0.007500558516544277
60-61	0.0032308159922142753
62-63	0.005898441669031429
64-65	0.006513889411693419
66-67	0.004712434460323388
68-69	0.003744413977916101
70-71	0.0052599796998841
72-73	0.0032682849072896516
74-75	0.002999620996440337
76-77	0.0024834913564274652
78-79	5.411745524572548E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.078761018308417E-5
88-89	0.0
90-91	1.8371614616645554E-4
92-93	0.0
94-95	1.0214037851073952E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	710.0
22-23	1477.0
24-25	1821.0
26-27	4286.0
28-29	9925.0
30-31	24794.0
32-33	31526.0
34-35	43712.0
36-37	68016.0
38-39	79870.0
40-41	79207.0
42-43	91886.0
44-45	109042.0
46-47	108647.0
48-49	99981.0
50-51	91210.0
52-53	87034.0
54-55	94153.0
56-57	97341.0
58-59	98080.0
60-61	96231.0
62-63	93226.0
64-65	99724.0
66-67	107293.0
68-69	107667.0
70-71	113100.0
72-73	96208.0
74-75	98890.0
76-77	103509.0
78-79	106882.0
80-81	107833.0
82-83	103029.0
84-85	101765.0
86-87	106024.0
88-89	110441.0
90-91	112894.0
92-93	110667.0
94-95	119644.0
96-97	184612.0
98-99	1008248.0
100-101	8017671.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.79636687509488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10774981084958	74.66905694564117
2	7.447975120532805	12.482265113534915
3	1.899598294384286	4.775383067745903
4	0.6996542171939272	2.3451392587875852
5	0.32528790635039656	1.3628972370284669
6	0.1696696561143933	0.8530620450799732
7	0.10354082403160139	0.6073441413971198
8	0.06259672094365817	0.4196302234698172
9	0.03875373964499639	0.2922680326566624
>10	0.14083433959838057	1.836400922978421
>50	0.003440825121977634	0.19362556562357783
>100	8.300668748225907E-4	0.11200266228366955
>500	3.424313920496062E-5	0.016513683109219288
>1k	3.4235220012036455E-5	0.03441110066347518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4334040183125007E-5	0.0	0.0	0.0
3	0.0	2.4334040183125007E-5	0.0	0.0	0.0
4	0.0	4.0556733638541684E-5	0.0	1.6222693455416674E-5	0.0
5	0.0	4.0556733638541684E-5	0.0	1.6222693455416674E-5	0.0
6	0.0	4.0556733638541684E-5	0.0	1.6222693455416674E-5	0.0
7	0.0	4.0556733638541684E-5	0.0	1.6222693455416674E-5	0.0
8	0.0	4.0556733638541684E-5	0.0	1.6222693455416674E-5	0.0
9	0.0	5.677942709395835E-5	0.0	2.4334040183125007E-5	8.111346727708337E-6
10-11	0.0	5.677942709395835E-5	0.0	3.244538691083335E-5	1.6222693455416674E-5
12-13	0.0	6.083510045781252E-5	0.0	3.244538691083335E-5	1.6222693455416674E-5
14-15	0.0	1.0950318082406254E-4	0.0	4.0556733638541684E-5	2.027836681927084E-5
16-17	0.0	1.135588541879167E-4	0.0	4.0556733638541684E-5	2.4334040183125007E-5
18-19	0.0	1.297815476433334E-4	0.0	6.894644718552086E-5	3.244538691083335E-5
20-21	0.0	1.4600424109875005E-4	0.0	7.705779391322919E-5	4.8668080366250014E-5
22-23	0.0	1.662826079180209E-4	0.0	8.516914064093753E-5	5.677942709395835E-5
24-25	4.055673363854168E-6	1.784496280095834E-4	0.0	1.0544750746020837E-4	6.48907738216667E-5
26-27	8.111346727708337E-6	1.8656097473729172E-4	0.0	1.8250530137343756E-4	6.48907738216667E-5
28-29	8.111346727708337E-6	1.8656097473729172E-4	0.0	5.31293210664896E-4	7.300212054937502E-5
30-31	8.111346727708337E-6	2.1900636164812507E-4	0.0	0.0015249331848091671	8.516914064093753E-5
32-33	8.111346727708337E-6	2.1900636164812507E-4	0.0	0.003641994680741043	8.92248140047917E-5
34-35	8.111346727708337E-6	2.514517485589584E-4	0.0	0.006553968155988335	9.733616073250003E-5
36-37	8.111346727708337E-6	2.8795280883364596E-4	0.0	0.010950318082406253	9.733616073250003E-5
38-39	8.111346727708337E-6	3.001198289252084E-4	0.0	0.01706627351509834	9.733616073250003E-5
40-41	8.111346727708337E-6	3.568992560191668E-4	0.0	0.023758134565457716	1.0950318082406254E-4
42-43	8.111346727708337E-6	3.6906627611072926E-4	0.0	0.030312102721446053	1.135588541879167E-4
44-45	8.111346727708337E-6	4.055673363854168E-4	0.0	0.03768937257029678	1.135588541879167E-4
46-47	8.111346727708337E-6	4.299013765685418E-4	0.0	0.045812886318096685	1.135588541879167E-4
48-49	8.111346727708337E-6	4.299013765685418E-4	0.0	0.054268965281732626	1.135588541879167E-4
50-51	8.111346727708337E-6	4.542354167516668E-4	0.0	0.06240870172298794	1.135588541879167E-4
52-53	8.111346727708337E-6	4.542354167516668E-4	0.0	0.07021587294840721	1.135588541879167E-4
54-55	8.111346727708337E-6	4.542354167516668E-4	0.0	0.07822988351538304	1.135588541879167E-4
56-57	8.111346727708337E-6	4.7856945693479185E-4	0.0	0.08687657912712013	1.1761452755177087E-4
58-59	8.111346727708337E-6	4.7856945693479185E-4	0.0	0.09454991313153221	1.2167020091562504E-4
60-61	8.111346727708337E-6	4.7856945693479185E-4	0.0	0.10200018510093233	1.2167020091562504E-4
62-63	8.111346727708337E-6	4.866808036625002E-4	0.0	0.10876099259847721	1.297815476433334E-4
64-65	8.111346727708337E-6	5.596829242118751E-4	0.0	0.11518517920682222	1.3789289437104172E-4
66-67	1.6222693455416674E-5	5.596829242118751E-4	0.0	0.12197843209127796	1.3789289437104172E-4
68-69	1.6222693455416674E-5	5.759056176672919E-4	0.0	0.12833367225243744	1.3789289437104172E-4
70-71	1.6222693455416674E-5	5.840169643950002E-4	0.0	0.1345307411524066	1.3789289437104172E-4
72-73	1.6222693455416674E-5	5.840169643950002E-4	0.0	0.1390284829129209	1.4600424109875005E-4
74-75	1.6222693455416674E-5	5.961839844865627E-4	0.0	0.1425771971062933	1.500599144626042E-4
76-77	1.6222693455416674E-5	6.245736980335419E-4	0.0	0.1459474616716561	1.5411558782645838E-4
78-79	1.6222693455416674E-5	6.28629371397396E-4	0.0	0.14898516102118287	1.5411558782645838E-4
80-81	1.6222693455416674E-5	6.326850447612502E-4	0.0	0.1500964155228789	1.5411558782645838E-4
82-83	1.6222693455416674E-5	6.326850447612502E-4	0.0	0.15067232114054618	1.5411558782645838E-4
84-85	1.6222693455416674E-5	6.489077382166669E-4	0.0	0.15120767002457497	1.5411558782645838E-4
86-87	1.6222693455416674E-5	6.489077382166669E-4	0.0	0.15137800830585682	1.6222693455416674E-4
88-89	1.6222693455416674E-5	6.732417783997919E-4	0.0	0.15143884340631464	1.7033828128187506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18160	0.0	50.656204	1
GTATCAA	32155	0.0	36.20137	1
TCAACGC	39975	0.0	29.044443	4
ATCAACG	40145	0.0	28.900074	3
CAACGCA	41045	0.0	28.308191	5
TATCAAC	41635	0.0	28.020403	2
AACGCAG	42395	0.0	27.431105	6
ACGCAGA	47875	0.0	24.300167	7
CGCAGAG	48410	0.0	24.021784	8
TGGTATC	8010	0.0	22.71209	2
GTGGTAT	8450	0.0	22.595764	1
GCAGAGT	56030	0.0	20.724241	9
GAGTACT	33975	0.0	19.903198	12-13
TACATGG	38515	0.0	19.584509	2
GTACATG	38975	0.0	19.463451	1
CTTATAC	9005	0.0	19.440176	1
AGAGTAC	51415	0.0	19.04416	10-11
ACATGGG	38610	0.0	18.902893	3
CAGAGTA	54885	0.0	18.88702	10-11
GTACTTT	37415	0.0	17.999428	14-15
>>END_MODULE