FastQCFastQC Report
Fri 27 May 2016
SRR2139692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11129272
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147150.048.8125951
GTATCAA265050.036.9526441
TCAACGC329650.029.4362244
ATCAACG331500.029.2328853
CAACGCA336650.028.7728635
TATCAAC345900.028.2030932
AACGCAG348650.027.879316
CTTATAC92400.027.0486411
ACGCAGA397050.024.4699847
CGCAGAG400100.024.2603558
GTGGTAT72850.023.8196491
TATACAC137000.021.9924553
TGGTATC72100.021.9720042
GCAGAGT468250.020.7570959
GAGTACT296850.019.47380812-13
AGAGTAC429050.019.18463910-11
TTATACA145850.018.8822212
CAGAGTA460850.018.35697710-11
GTACATG303800.018.1443731
GCGAATC68900.018.08825332-33
Produced by FastQC (version 0.11.5)