##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139692_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11129272
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.198063628959737	31.0	25.0	33.0	18.0	33.0
2	30.937771131840428	33.0	31.0	33.0	27.0	33.0
3	31.06454653997135	33.0	31.0	33.0	27.0	34.0
4	31.86685059004758	33.0	32.0	33.0	29.0	34.0
5	32.324277724544785	33.0	33.0	34.0	31.0	34.0
6	35.90167056749085	38.0	36.0	38.0	31.0	38.0
7	36.209419627806746	38.0	37.0	38.0	33.0	38.0
8	36.46570260840062	38.0	37.0	38.0	34.0	38.0
9	36.67943024485339	38.0	38.0	38.0	34.0	38.0
10-11	36.70930695197313	38.0	38.0	38.0	34.0	38.0
12-13	36.7592278722274	38.0	38.0	38.0	34.0	38.0
14-15	36.76967518630149	38.0	38.0	38.0	34.0	38.0
16-17	36.757040577317184	38.0	38.0	38.0	34.0	38.0
18-19	36.76311249289262	38.0	38.0	38.0	34.5	38.0
20-21	36.79075541440942	38.0	38.0	38.0	35.0	38.0
22-23	36.76935008252461	38.0	38.0	38.0	34.5	38.0
24-25	36.77607146849876	38.0	38.0	38.0	35.0	38.0
26-27	36.74397650731636	38.0	38.0	38.0	34.5	38.0
28-29	36.66610321231559	38.0	38.0	38.0	34.0	38.0
30-31	36.688844982856224	38.0	38.0	38.0	34.0	38.0
32-33	36.68396183512809	38.0	38.0	38.0	34.0	38.0
34-35	36.66869843892508	38.0	38.0	38.0	34.0	38.0
36-37	36.63886361416509	38.0	38.0	38.0	34.0	38.0
38-39	36.621702899108726	38.0	38.0	38.0	34.0	38.0
40-41	36.63507295342957	38.0	38.0	38.0	34.0	38.0
42-43	36.61581633449323	38.0	38.0	38.0	34.0	38.0
44-45	36.55759674483707	38.0	38.0	38.0	34.0	38.0
46-47	36.4410639841603	38.0	38.0	38.0	34.0	38.0
48-49	36.39501528087166	38.0	38.0	38.0	34.0	38.0
50-51	36.40447123861984	38.0	38.0	38.0	34.0	38.0
52-53	36.406300630350785	38.0	38.0	38.0	34.0	38.0
54-55	36.334036382668465	38.0	38.0	38.0	33.0	38.0
56-57	36.350755628602414	38.0	38.0	38.0	34.0	38.0
58-59	36.29270450325284	38.0	38.0	38.0	33.5	38.0
60-61	36.307587095089076	38.0	38.0	38.0	33.0	38.0
62-63	36.32294501571366	38.0	38.0	38.0	33.0	38.0
64-65	36.31211431587509	38.0	38.0	38.0	33.0	38.0
66-67	36.293074218322104	38.0	38.0	38.0	33.0	38.0
68-69	36.27254029919142	38.0	37.5	38.0	33.0	38.0
70-71	36.273728889903126	38.0	37.0	38.0	33.0	38.0
72-73	36.2626606773486	38.0	37.0	38.0	33.0	38.0
74-75	36.22604910219411	38.0	37.0	38.0	33.0	38.0
76-77	36.216270889954245	38.0	37.0	38.0	33.0	38.0
78-79	36.20321974862157	38.0	37.0	38.0	33.0	38.0
80-81	36.21279896006094	38.0	37.0	38.0	33.0	38.0
82-83	36.183520358016516	38.0	37.0	38.0	33.0	38.0
84-85	36.151308086375835	38.0	37.0	38.0	33.0	38.0
86-87	36.152703138781206	38.0	37.0	38.0	33.0	38.0
88-89	36.156356960100595	38.0	37.0	38.0	33.0	38.0
90-91	36.103288329017644	38.0	37.0	38.0	33.0	38.0
92-93	36.124682184472405	38.0	37.0	38.0	33.0	38.0
94-95	36.075900213621566	38.0	37.0	38.0	33.0	38.0
96-97	36.10393039521831	38.0	37.0	38.0	33.0	38.0
98-99	36.15290030699339	38.0	37.0	38.0	33.0	38.0
100-101	35.606281733918934	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	25.0
19	46.0
20	146.0
21	620.0
22	2539.0
23	7453.0
24	16886.0
25	31515.0
26	52725.0
27	80280.0
28	114282.0
29	152666.0
30	196063.0
31	249430.0
32	315406.0
33	410718.0
34	564446.0
35	878741.0
36	1849222.0
37	6206058.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.62850400277754	20.758419778041187	15.727335983881066	23.885740235300208
2	14.397770132673548	21.918360877512924	41.50207668569876	22.18179230411477
3	18.030451587489278	26.429329789046403	31.03912816579557	24.501090457668752
4	12.740024684453754	18.92646706810652	38.025236511426804	30.30827173601292
5	13.585732947225427	37.26884096603695	35.528802109508526	13.616623977229104
6	29.515488524316773	38.455992449461206	18.58684916677389	13.44166985944813
7	26.035041555316198	32.73263011325198	23.107106442945266	18.12522188848656
8	23.75215557675291	37.58357240257943	21.02901249964957	17.63525952101809
9	24.774082258030894	17.427941378375873	21.514336247689876	36.28364011590336
10-11	23.936565662156518	27.21756193936135	28.756710232259575	20.08916216622255
12-13	24.801068497706115	24.53859397163543	29.06588980832122	21.594447722337236
14-15	21.865603608214446	26.029649558389806	26.759283985511363	25.34546284788439
16-17	21.412510180360407	29.202858012635506	27.466266436834324	21.918365370169766
18-19	21.40577119509704	28.106362213089948	29.401267216759553	21.086599375053463
20-21	22.097293924580537	27.225943489069444	29.45147027592336	21.225292310426664
22-23	21.834508184343317	27.265980340825934	29.281536997978264	21.617974476852485
24-25	21.659055110786095	27.398707321405613	29.403632166493715	21.53860540131457
26-27	21.700855409187177	27.540268482316094	29.302524124955216	21.456351983541513
28-29	21.526094988506177	27.44783717941478	29.422842295973407	21.60322553610564
30-31	21.7960823354048	27.458594666993108	29.258344974832756	21.486978022769332
32-33	21.802829659722764	27.45955171366067	29.235685057972788	21.501933568643782
34-35	21.787213442937052	27.43303072824905	29.27280249119692	21.506953337616974
36-37	21.666781327378963	27.53498951807467	29.21121719395165	21.58701196059471
38-39	21.54808584171682	27.462081853565728	29.383062286362904	21.60677001835455
40-41	21.81856081336708	27.309353317343188	29.27704122298908	21.595044646300657
42-43	21.774087725267538	27.396278840593318	29.11648939247233	21.713144041666812
44-45	21.75915964804592	27.57501210567188	28.938155913977994	21.727672332304206
46-47	21.955970093886265	27.52721405636181	28.539160912059458	21.977654937692463
48-49	21.851154049181627	27.59789529299561	28.605863198970134	21.94508745885263
50-51	21.754295420445793	27.721271481875657	28.584241144615515	21.940191953063035
52-53	21.934394006731196	27.64676198906647	28.584032360205363	21.83481164399697
54-55	21.88851842373439	27.654280874030903	28.437673037639673	22.01952766459504
56-57	22.012053629058926	27.82100285198127	28.25915424491801	21.907789274041797
58-59	21.85319191591328	27.965973249947616	28.44546999095432	21.735364843184783
60-61	21.954304961130568	28.067382612966163	28.268658781075974	21.7096536448273
62-63	21.82514138391343	28.22477647508536	28.23290979225877	21.717172348742434
64-65	21.79804640483151	28.18342940179538	28.203252078847157	21.815272114525957
66-67	21.889036269417396	28.121655123253287	28.106032764615023	21.883275842714298
68-69	21.869795566592337	27.996101380551202	28.25145941267858	21.88264364017788
70-71	21.891370822581422	27.741731633427253	28.319375907778838	22.04752163621249
72-73	21.970047884339476	27.697485069025024	28.303703922449724	22.028763124185772
74-75	21.906094380116762	27.80592971890663	28.225511307687047	22.06246459328956
76-77	22.00545661363186	27.752974664944404	28.189419393585247	22.052149327838492
78-79	21.948598791659006	27.776137909788858	28.162497996440738	22.1127653021114
80-81	21.93998354406472	27.80623909554659	28.191768503287594	22.06200885710109
82-83	22.00800324236009	27.79176715831862	28.21742319626528	21.982806403056014
84-85	22.094498085026768	27.78606606990963	28.129163589110473	21.990272255953126
86-87	21.973479703696288	27.789803068434267	28.213742486328414	22.02297474154103
88-89	22.043220121578738	27.777427854258583	28.13905727580288	22.04029474835979
90-91	22.06216657102764	27.831598733151182	28.127262900478033	21.978971795343146
92-93	22.07175900964673	27.794389411162047	28.146677398910402	21.987174180280824
94-95	21.97182659238412	27.889007971023755	28.128563204527225	22.010602232064905
96-97	22.036473288465373	27.88027322800703	28.132650293016265	21.95060319051133
98-99	22.265777881213275	28.448242635168842	28.186982213567465	21.09899727005042
100-101	22.818668675396665	30.991941582896725	25.578828171618547	20.610561570088066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2911.0
1	2805.0
2	3305.5
3	4176.5
4	5461.5
5	8062.0
6	10477.0
7	13422.0
8	16272.5
9	17116.0
10	16223.0
11	15243.0
12	16500.5
13	20683.0
14	29787.5
15	41128.5
16	49264.5
17	52911.0
18	52548.0
19	49299.5
20	45073.5
21	41786.0
22	41944.0
23	47298.0
24	58117.5
25	74492.5
26	95116.0
27	117684.5
28	141480.0
29	166532.5
30	190292.0
31	215339.0
32	244121.5
33	272148.0
34	297659.5
35	321111.0
36	347174.0
37	371300.5
38	389594.5
39	403557.0
40	416787.5
41	432474.5
42	441275.0
43	451244.0
44	472133.0
45	483206.0
46	478306.0
47	473086.0
48	466772.5
49	455161.0
50	440773.0
51	423084.0
52	400835.5
53	377634.0
54	353017.0
55	324879.0
56	295969.0
57	264932.0
58	233411.0
59	201999.5
60	168090.0
61	135007.5
62	105505.5
63	80934.0
64	60763.5
65	44441.0
66	32487.5
67	24626.5
68	18563.0
69	13478.5
70	9827.5
71	7001.5
72	4895.0
73	3308.0
74	2133.5
75	1348.5
76	812.5
77	469.5
78	315.0
79	212.0
80	142.5
81	99.5
82	70.0
83	45.5
84	27.5
85	24.0
86	19.0
87	11.0
88	4.5
89	3.5
90	4.0
91	3.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.056625446839649526
6	0.0
7	0.0033335513769454104
8	0.0
9	0.0
10-11	0.0
12-13	1.3477970526733464E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.494298398223062E-5
28-29	2.697657596932008E-5
30-31	2.250289330950727E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.3965395333109359E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	8.221138431519287E-4
54-55	1.1137346826374284E-4
56-57	0.0030990878432788837
58-59	0.007607200013069031
60-61	0.003319264579968898
62-63	0.005867048773431682
64-65	0.0066205677492328615
66-67	0.004879840665823983
68-69	0.0036641363227272604
70-71	0.005307402190212205
72-73	0.0031680258494125066
74-75	0.003010488350944426
76-77	0.0025841840673733094
78-79	7.008745944496986E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	5.049366534526615E-5
88-89	5.66932762227946E-6
90-91	1.71960795002269E-4
92-93	0.0
94-95	8.795313528593593E-5
96-97	5.940100501748409E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	127.0
20-21	584.0
22-23	1202.0
24-25	1566.0
26-27	3460.0
28-29	7812.0
30-31	20621.0
32-33	25899.0
34-35	36741.0
36-37	57488.0
38-39	67273.0
40-41	67108.0
42-43	76540.0
44-45	91228.0
46-47	92353.0
48-49	85134.0
50-51	76158.0
52-53	73033.0
54-55	79241.0
56-57	83317.0
58-59	83756.0
60-61	82173.0
62-63	79619.0
64-65	88170.0
66-67	95968.0
68-69	95225.0
70-71	103947.0
72-73	82501.0
74-75	84357.0
76-77	89473.0
78-79	92674.0
80-81	93685.0
82-83	88455.0
84-85	87875.0
86-87	91523.0
88-89	95539.0
90-91	99090.0
92-93	95992.0
94-95	103773.0
96-97	165099.0
98-99	917825.0
100-101	7365668.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.16597934780914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62300198439134	76.3283073609133
2	7.311359791408088	12.453582339989268
3	1.74313402546681	4.453671492401093
4	0.6183390867332881	2.1064581556268154
5	0.27449960605415635	1.1689013890095012
6	0.15132338597474396	0.7732562618859357
7	0.08796946744137994	0.5244404093145233
8	0.0514313837424015	0.350416333250769
9	0.033126447516429895	0.25391217105454966
>10	0.10317646215049277	1.3739049569491648
>50	0.002052493056337514	0.11245171458529417
>100	5.517843930520973E-4	0.07617049555393993
>500	3.4081671495358295E-5	0.02452691946585677
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7970627368977954E-5
2	0.0	8.985313684488977E-6	0.0	0.0	2.6955941053466928E-5
3	0.0	8.985313684488977E-6	0.0	0.0	2.6955941053466928E-5
4	0.0	3.594125473795591E-5	0.0	8.985313684488977E-6	2.6955941053466928E-5
5	0.0	3.594125473795591E-5	0.0	8.985313684488977E-6	2.6955941053466928E-5
6	6.289719579142283E-5	3.594125473795591E-5	0.0	8.985313684488977E-6	2.6955941053466928E-5
7	6.289719579142283E-5	4.492656842244488E-5	0.0	4.492656842244488E-5	2.6955941053466928E-5
8	6.289719579142283E-5	5.3911882106933856E-5	0.0	4.492656842244488E-5	2.6955941053466928E-5
9	6.289719579142283E-5	1.0782376421386771E-4	0.0	4.492656842244488E-5	3.594125473795591E-5
10-11	6.289719579142283E-5	1.0782376421386771E-4	0.0	4.492656842244488E-5	3.594125473795591E-5
12-13	6.289719579142283E-5	1.0782376421386771E-4	0.0	4.941922526468937E-5	4.0433911580200394E-5
14-15	6.738985263366733E-5	1.7521361684753504E-4	0.0	5.3911882106933856E-5	5.840453894917834E-5
16-17	1.3927236210957916E-4	1.9767690105875748E-4	0.0	5.840453894917834E-5	6.289719579142283E-5
18-19	1.4376501895182363E-4	2.1115487158549095E-4	0.0	6.289719579142283E-5	8.985313684488976E-5
20-21	1.4376501895182363E-4	2.336181557967134E-4	0.0	7.188250947591182E-5	9.434579368713425E-5
22-23	1.4376501895182363E-4	2.4709612632344685E-4	0.0	9.434579368713425E-5	1.0333110737162322E-4
24-25	1.5724298947855707E-4	3.055006652726252E-4	0.0	1.3028704842509017E-4	1.0782376421386771E-4
26-27	1.6173564632080158E-4	3.324566063260921E-4	0.0	2.4709612632344685E-4	1.2579439158284567E-4
28-29	1.6173564632080158E-4	3.3694926316833664E-4	0.0	6.199866442297393E-4	1.2579439158284567E-4
30-31	1.7072096000529056E-4	3.5941254737955903E-4	0.0	0.0018914085305849295	1.3028704842509014E-4
32-33	1.7521361684753504E-4	3.5941254737955903E-4	0.0	0.0042365754022365525	1.4376501895182363E-4
34-35	1.9767690105875748E-4	4.088317726442484E-4	0.0	0.007341001280227494	1.6173564632080158E-4
36-37	2.0666221474324647E-4	4.4028037053995986E-4	0.0	0.012107710189848896	1.6173564632080158E-4
38-39	2.0666221474324647E-4	4.6274365475118225E-4	0.0	0.019381321617442722	1.8419893053202402E-4
40-41	2.0666221474324647E-4	5.301335073848496E-4	0.0	0.027203037179790376	1.9767690105875748E-4
42-43	2.0666221474324647E-4	5.391188210693386E-4	0.0	0.0343149129610634	1.9767690105875748E-4
44-45	2.1115487158549095E-4	6.020160168607614E-4	0.0	0.0421815550918335	1.9767690105875748E-4
46-47	2.1564752842773542E-4	6.469425852832063E-4	0.0	0.051256721913167366	2.0666221474324647E-4
48-49	2.2014018526997993E-4	6.514352421254508E-4	0.0	0.06071376456609201	2.1564752842773542E-4
50-51	2.4709612632344685E-4	6.783911831789178E-4	0.0	0.07000008625901137	2.1564752842773542E-4
52-53	2.5158878316569133E-4	6.828838400211622E-4	0.0	0.07890453212033995	2.3361815579671337E-4
54-55	2.560814400079358E-4	6.963618105478957E-4	0.0	0.08777752938377281	2.5158878316569133E-4
56-57	2.6057409685018034E-4	7.008544673901401E-4	0.0	0.09445811010819036	2.560814400079358E-4
58-59	2.6057409685018034E-4	7.053471242323846E-4	0.0	0.09997509271046659	2.6057409685018034E-4
60-61	2.6057409685018034E-4	7.143324379168736E-4	0.0	0.10544714874432037	2.695594105346693E-4
62-63	3.144859789571142E-4	7.323030652858516E-4	0.0	0.11104949182659926	2.7854472421915826E-4
64-65	3.144859789571142E-4	8.176635452884968E-4	0.0	0.11620256922465369	2.7854472421915826E-4
66-67	3.2796394948384763E-4	8.176635452884968E-4	0.0	0.12166563994482299	2.830373810614028E-4
68-69	3.324566063260921E-4	8.536048000264527E-4	0.0	0.12697596033235597	2.8753003790364727E-4
70-71	3.5491989053731455E-4	8.580974568686972E-4	0.0	0.132250339465151	2.965153515881362E-4
72-73	4.043391158020039E-4	8.715754273954308E-4	0.0	0.1361185170063235	3.77383174748537E-4
74-75	4.088317726442484E-4	9.2099465266012E-4	0.0	0.13942061978537318	3.77383174748537E-4
76-77	4.1332442948649294E-4	9.47950593713587E-4	0.0	0.14250707503599516	3.77383174748537E-4
78-79	4.1332442948649294E-4	9.524432505558315E-4	0.0	0.1453688974445049	3.77383174748537E-4
80-81	4.1332442948649294E-4	9.56935907398076E-4	0.0	0.14646959837085483	3.8187583159078153E-4
82-83	4.178170863287374E-4	9.614285642403204E-4	0.0	0.14692784936876374	3.86368488433026E-4
84-85	4.3129505685547085E-4	9.74906534767054E-4	0.0	0.14736363708246147	3.86368488433026E-4
86-87	4.357877136977154E-4	0.0010063551326627653	0.0	0.14750290944457103	3.9535380211751497E-4
88-89	4.4028037053995986E-4	0.0010243257600317432	0.0	0.14755682132667797	3.9984645895975945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14715	0.0	48.812595	1
GTATCAA	26505	0.0	36.952644	1
TCAACGC	32965	0.0	29.436224	4
ATCAACG	33150	0.0	29.232885	3
CAACGCA	33665	0.0	28.772863	5
TATCAAC	34590	0.0	28.203093	2
AACGCAG	34865	0.0	27.87931	6
CTTATAC	9240	0.0	27.048641	1
ACGCAGA	39705	0.0	24.469984	7
CGCAGAG	40010	0.0	24.260355	8
GTGGTAT	7285	0.0	23.819649	1
TATACAC	13700	0.0	21.992455	3
TGGTATC	7210	0.0	21.972004	2
GCAGAGT	46825	0.0	20.757095	9
GAGTACT	29685	0.0	19.473808	12-13
AGAGTAC	42905	0.0	19.184639	10-11
TTATACA	14585	0.0	18.882221	2
CAGAGTA	46085	0.0	18.356977	10-11
GTACATG	30380	0.0	18.144373	1
GCGAATC	6890	0.0	18.088253	32-33
>>END_MODULE