FastQCFastQC Report
Fri 27 May 2016
SRR2139691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11651554
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148400.050.596431
GTATCAA279300.039.0041161
TCAACGC332650.032.3572654
ATCAACG337700.031.8476893
CAACGCA343900.031.2356645
TATCAAC348350.031.0484492
AACGCAG355200.030.2709476
ACGCAGA409050.026.275287
CGCAGAG413550.025.9573468
GTGGTAT74650.022.7910271
TGGTATC70550.022.6390672
GCAGAGT483050.022.2855229
GAGTACT284200.020.9078612-13
GTACATG342900.020.1503811
TACATGG346350.019.6363832
CAGAGTA471700.019.16218210-11
ACATGGG344600.019.0056043
AGAGTAC437650.018.95837810-11
GTACTTT318850.018.57487314-15
AGTACTT311650.017.38234912-13
Produced by FastQC (version 0.11.5)