##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11651554
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.895942893111084	33.0	31.0	33.0	25.0	34.0
2	31.870784532260675	33.0	32.0	33.0	28.0	34.0
3	31.65461448318396	33.0	31.0	33.0	28.0	34.0
4	32.24019594296177	33.0	33.0	34.0	30.0	34.0
5	32.26198591192213	33.0	33.0	34.0	31.0	34.0
6	35.593650769674156	38.0	36.0	38.0	29.0	38.0
7	36.27259110673134	38.0	37.0	38.0	33.0	38.0
8	36.421228103993684	38.0	37.0	38.0	34.0	38.0
9	36.62550815110156	38.0	38.0	38.0	34.0	38.0
10-11	36.68920227293286	38.0	38.0	38.0	34.0	38.0
12-13	36.732535419738866	38.0	38.0	38.0	34.0	38.0
14-15	36.778875032463475	38.0	38.0	38.0	35.0	38.0
16-17	36.76069187852539	38.0	38.0	38.0	34.0	38.0
18-19	36.76343614765893	38.0	38.0	38.0	34.5	38.0
20-21	36.78925577891034	38.0	38.0	38.0	35.0	38.0
22-23	36.76272477485149	38.0	38.0	38.0	34.5	38.0
24-25	36.7704355061559	38.0	38.0	38.0	34.5	38.0
26-27	36.737798746687794	38.0	38.0	38.0	34.5	38.0
28-29	36.66171362349794	38.0	38.0	38.0	34.0	38.0
30-31	36.68600457797235	38.0	38.0	38.0	34.0	38.0
32-33	36.681536113887745	38.0	38.0	38.0	34.0	38.0
34-35	36.66993991666193	38.0	38.0	38.0	34.0	38.0
36-37	36.64343917672761	38.0	38.0	38.0	34.0	38.0
38-39	36.6305872473159	38.0	38.0	38.0	34.0	38.0
40-41	36.6283946259956	38.0	38.0	38.0	34.0	38.0
42-43	36.599141647906166	38.0	38.0	38.0	34.0	38.0
44-45	36.54172689517337	38.0	38.0	38.0	34.0	38.0
46-47	36.42711444812525	38.0	38.0	38.0	34.0	38.0
48-49	36.381481901605255	38.0	38.0	38.0	34.0	38.0
50-51	36.39285320560051	38.0	38.0	38.0	34.0	38.0
52-53	36.39819568549996	38.0	38.0	38.0	34.0	38.0
54-55	36.31752062482461	38.0	38.0	38.0	33.0	38.0
56-57	36.3324039334548	38.0	38.0	38.0	33.0	38.0
58-59	36.27469536726747	38.0	38.0	38.0	33.0	38.0
60-61	36.28542023042742	38.0	38.0	38.0	33.0	38.0
62-63	36.304954814460785	38.0	38.0	38.0	33.0	38.0
64-65	36.29183198433569	38.0	38.0	38.0	33.0	38.0
66-67	36.273562996222886	38.0	37.0	38.0	33.0	38.0
68-69	36.253496599661304	38.0	37.0	38.0	33.0	38.0
70-71	36.25440475805438	38.0	37.0	38.0	33.0	38.0
72-73	36.245203043030955	38.0	37.0	38.0	33.0	38.0
74-75	36.204726762330935	38.0	37.0	38.0	33.0	38.0
76-77	36.191187958533234	38.0	37.0	38.0	33.0	38.0
78-79	36.17648773975637	38.0	37.0	38.0	33.0	38.0
80-81	36.18245385033114	38.0	37.0	38.0	33.0	38.0
82-83	36.14690925665832	38.0	37.0	38.0	33.0	38.0
84-85	36.110803276625624	38.0	37.0	38.0	33.0	38.0
86-87	36.11316598113927	38.0	37.0	38.0	33.0	38.0
88-89	36.11471890647083	38.0	37.0	38.0	33.0	38.0
90-91	36.06035006579063	38.0	37.0	38.0	33.0	38.0
92-93	36.08173126700082	38.0	37.0	38.0	33.0	38.0
94-95	36.03010581885762	38.0	37.0	38.0	32.5	38.0
96-97	36.05092293133485	38.0	37.0	38.0	33.0	38.0
98-99	36.097408024275296	38.0	37.0	38.0	33.0	38.0
100-101	35.55613651170694	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	10.0
19	34.0
20	99.0
21	573.0
22	2463.0
23	7495.0
24	17514.0
25	33556.0
26	55745.0
27	83503.0
28	118187.0
29	157841.0
30	203404.0
31	258852.0
32	327874.0
33	425155.0
34	583364.0
35	897830.0
36	1811538.0
37	6666515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03514895952935	20.558038867605127	14.062278731231903	25.344533441633622
2	14.227321093821477	20.697196270986687	42.66178571545049	22.413696919741348
3	18.2195439337963	26.00889117451629	29.49985040622049	26.27171448546692
4	12.624547764186648	18.37487085413671	38.69767071413822	30.302910667538423
5	13.877366015393399	36.93127520079914	35.23476203987609	13.956596743931367
6	29.887884483048353	37.98507907185599	18.59395750987379	13.533078935221859
7	26.567973580604193	32.63949260917626	22.75855995856885	18.0339738516507
8	24.059125503774005	37.15454608029109	20.878227917065825	17.90810049886908
9	25.260003944538212	17.383200558483445	21.285109265253375	36.07168623172497
10-11	24.148950431847975	27.03144576251374	28.54824343602579	20.271360369612502
12-13	24.993964323897426	24.381947196223543	28.79312740541969	21.830961074459346
14-15	22.141990673518745	25.750595156663223	26.580737642378004	25.526676527440028
16-17	21.650687110062744	28.93259989182559	27.238079143777732	22.17863385433394
18-19	21.65362234084827	27.95392786232635	29.11418082085875	21.27826897596664
20-21	22.449603940150194	27.087532692076206	29.06420360074695	21.398659767026647
22-23	22.146262079016772	27.043921024854672	28.92193145819828	21.887885437930272
24-25	21.8957334987199	27.19184770129126	29.07149034613148	21.840928453857362
26-27	21.894960700171566	27.309367157376514	28.982533142489007	21.81313899996291
28-29	21.787114846419488	27.313703956505474	29.04336330274788	21.855817894327153
30-31	22.13857089538048	27.191058894209853	28.94026348964669	21.730106720762986
32-33	21.89010315142184	27.348098099110814	28.942127283142522	21.81967146632483
34-35	22.021052429891512	27.36640249753732	28.929577113479983	21.682967959091194
36-37	21.97300188567415	27.244284404805242	28.91723881732693	21.865474892193674
38-39	21.75921320426894	27.39210655696632	29.016566721331383	21.832113517433363
40-41	22.057354895085226	27.202598252769484	28.919837549228223	21.82020930291707
42-43	21.99844159989403	27.225973828319866	28.800795529169047	21.974789042617054
44-45	21.99329779864003	27.385831042901593	28.65675790521573	21.96411325324265
46-47	22.12300958322405	27.360258025492968	28.349833864969604	22.166898526313382
48-49	22.01538357522762	27.479852159019202	28.345974939150814	22.158789326602363
50-51	21.945319554179115	27.58047725989517	28.322189052494156	22.152014133431557
52-53	22.13180622818006	27.507525504967123	28.248590799530092	22.112077467322724
54-55	22.107014256514205	27.450334065887557	28.216576274352214	22.226075403246025
56-57	22.131718346211343	27.528376099838148	28.180838591477254	22.159066962473254
58-59	22.03470811636777	27.60219815427607	28.288786750260638	22.074306979095525
60-61	22.094839294861853	27.628016599226434	28.232027295670196	22.045116810241517
62-63	21.981135261169158	27.77515687265655	28.221245396333188	22.022462469841106
64-65	21.962412586369535	27.726498869343864	28.175978655831535	22.13510988845507
66-67	22.029356106968844	27.843111938553587	27.957243927182173	22.170288027295395
68-69	22.023512113157583	27.742895244736136	28.03656345038667	22.19702919171961
70-71	22.027888342045507	27.485257965913007	28.11445843447145	22.372395257570034
72-73	22.08599731518954	27.45854447464977	28.048376893835115	22.407081316325574
74-75	21.993968005479672	27.557812273871367	28.021612865884553	22.426606854764405
76-77	22.18584187436819	27.539058406329154	27.927032597006647	22.348067122296012
78-79	22.19455959565204	27.543010266736367	27.8605958250345	22.40183431257709
80-81	22.177111030146257	27.606939879896654	27.808961576422707	22.406987513534386
82-83	22.17640986037454	27.613174679413927	27.856644048394802	22.353771411816734
84-85	22.339505683918322	27.610000980636208	27.784938727000103	22.265554608445374
86-87	22.18966343363979	27.614359637131642	27.870275418257446	22.325701510971122
88-89	22.256559527113634	27.66437506042744	27.82654640664309	22.252519005815838
90-91	22.249362200309246	27.645224847286293	27.8734430787887	22.23196987361576
92-93	22.317285632401603	27.69056832668492	27.80805809099567	22.184087949917803
94-95	22.27935687428861	27.742632776262187	27.838394692508533	22.139615656940673
96-97	22.29088621795464	27.71078792187064	27.85705959008322	22.1412662700915
98-99	22.482254151773244	28.261112008051896	27.971728221009702	21.284905619165155
100-101	23.030495995506627	30.800223687892153	25.34279149893086	20.826488817670363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2623.0
1	2424.5
2	2917.5
3	3840.5
4	4787.0
5	6977.5
6	9198.5
7	11909.0
8	15252.0
9	16892.5
10	16192.0
11	15021.5
12	15734.5
13	18966.5
14	26349.5
15	36043.5
16	43946.5
17	48471.0
18	48620.5
19	45464.0
20	41429.5
21	37836.0
22	37030.5
23	41486.0
24	51436.5
25	66571.5
26	86585.0
27	109018.5
28	133084.5
29	158594.5
30	183949.5
31	210666.5
32	238848.0
33	269250.0
34	299825.5
35	327022.5
36	356471.5
37	382729.5
38	401643.0
39	417162.0
40	433346.0
41	449111.0
42	458793.0
43	467254.5
44	475340.5
45	485081.0
46	489938.5
47	487429.0
48	484585.0
49	476991.5
50	465561.5
51	450905.0
52	430747.0
53	409753.5
54	387245.0
55	359533.5
56	328845.0
57	295910.5
58	262946.0
59	229922.5
60	193492.5
61	156619.0
62	122727.5
63	93601.5
64	70156.0
65	51807.5
66	37861.0
67	28080.5
68	20732.5
69	14727.0
70	10325.0
71	7118.5
72	4828.5
73	3163.0
74	2039.5
75	1267.5
76	801.0
77	485.0
78	301.5
79	186.5
80	123.5
81	91.5
82	64.0
83	43.0
84	26.5
85	17.5
86	12.0
87	7.5
88	5.0
89	2.5
90	2.5
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06078159188036206
6	0.0
7	0.0035789217472622105
8	0.0
9	0.0
10-11	0.0
12-13	8.582546156504103E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.726716676838746E-5
28-29	2.5763636327723417E-5
30-31	3.437960055459452E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.434667087546138E-6
46-47	0.0
48-49	0.0
50-51	0.0
52-53	7.726847792208694E-4
54-55	1.4261554423862873E-4
56-57	0.0029362427763333274
58-59	0.007381977659328307
60-61	0.0029043103066641513
62-63	0.005469896295502078
64-65	0.006404978586992042
66-67	0.004486512614008327
68-69	0.0034591444913804146
70-71	0.005116226138176367
72-73	0.003129144135508577
74-75	0.002946295916773432
76-77	0.0024838518143076413
78-79	5.595142602382209E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	8.463690924627553E-5
88-89	0.0
90-91	1.5668738235951856E-4
92-93	0.0
94-95	7.184349127535405E-5
96-97	5.599129536925671E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	527.0
22-23	1158.0
24-25	1325.0
26-27	2828.0
28-29	6342.0
30-31	19329.0
32-33	25651.0
34-35	35674.0
36-37	56663.0
38-39	63998.0
40-41	66506.0
42-43	75034.0
44-45	90219.0
46-47	91486.0
48-49	84837.0
50-51	77659.0
52-53	66391.0
54-55	71109.0
56-57	71931.0
58-59	82574.0
60-61	82657.0
62-63	80146.0
64-65	87733.0
66-67	94459.0
68-69	95310.0
70-71	99533.0
72-73	86532.0
74-75	86956.0
76-77	92824.0
78-79	96918.0
80-81	97158.0
82-83	92253.0
84-85	92058.0
86-87	95655.0
88-89	100259.0
90-91	103028.0
92-93	102293.0
94-95	111098.0
96-97	176110.0
98-99	988466.0
100-101	7798736.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.42884660252093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.01686807400915	76.90037214333893
2	7.079757098889257	12.09630966368238
3	1.6655607925797	4.268608123693932
4	0.5508186974155115	1.882232240292405
5	0.2636515359987067	1.1261723312676273
6	0.1351353551705413	0.6926674516464815
7	0.07938739729516828	0.47473816499916205
8	0.053460575671739965	0.36536602546748287
9	0.03486720931671839	0.26807989285583317
>10	0.11620243138178524	1.539641203862961
>50	0.003403612646451012	0.19431672218895496
>100	8.040852854798384E-4	0.11005511259346029
>500	3.326019649806754E-5	0.016664229208909677
>1k	4.987414327780614E-5	0.064776694901492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7165092313008206E-5
2	0.0	4.2912730782520514E-5	0.0	8.582546156504103E-6	1.7165092313008206E-5
3	8.582546156504103E-6	4.2912730782520514E-5	0.0	8.582546156504103E-6	1.7165092313008206E-5
4	8.582546156504103E-6	9.440800772154513E-5	0.0	1.7165092313008206E-5	1.7165092313008206E-5
5	8.582546156504103E-6	9.440800772154513E-5	0.0	1.7165092313008206E-5	1.7165092313008206E-5
6	1.7165092313008206E-5	9.440800772154513E-5	0.0	1.7165092313008206E-5	4.2912730782520514E-5
7	1.7165092313008206E-5	9.440800772154513E-5	0.0	1.7165092313008206E-5	4.2912730782520514E-5
8	1.7165092313008206E-5	1.0299055387804923E-4	0.0	1.7165092313008206E-5	4.2912730782520514E-5
9	1.7165092313008206E-5	1.5448583081707385E-4	0.0	1.7165092313008206E-5	6.866036925203282E-5
10-11	2.574763846951231E-5	1.5448583081707385E-4	0.0	2.574763846951231E-5	6.866036925203282E-5
12-13	3.433018462601641E-5	1.5448583081707385E-4	0.0	3.003891154776436E-5	6.866036925203282E-5
14-15	4.2912730782520514E-5	1.9310728852134232E-4	0.0	3.433018462601641E-5	1.1586437311280538E-4
16-17	5.149527693902462E-5	1.9739856159959435E-4	0.0	3.433018462601641E-5	1.330294654258136E-4
18-19	5.149527693902462E-5	2.2314620006910665E-4	0.0	4.7204003860772566E-5	1.3732073850406565E-4
20-21	5.149527693902462E-5	2.4889383853861894E-4	0.0	5.149527693902462E-5	1.4590328466056976E-4
22-23	5.149527693902462E-5	2.703502039298792E-4	0.0	8.153418848678897E-5	1.8881601544309024E-4
24-25	5.149527693902462E-5	3.175542077906518E-4	0.0	1.1157310003455332E-4	2.1885492699085462E-4
26-27	5.149527693902462E-5	3.3471930010366E-4	0.0	1.5448583081707385E-4	2.3172874622561076E-4
28-29	5.149527693902462E-5	3.3471930010366E-4	0.0	4.548749462947174E-4	2.3172874622561076E-4
30-31	5.149527693902462E-5	4.033796693556928E-4	0.0	0.001682179046674804	2.4031129238211485E-4
32-33	5.149527693902462E-5	4.1196221551219694E-4	0.0	0.004694652747607744	2.703502039298792E-4
34-35	5.149527693902462E-5	4.6345749245122153E-4	0.0	0.008415186506452272	3.046803885558957E-4
36-37	5.149527693902462E-5	5.149527693902461E-4	0.0	0.014723357931482788	3.1755420779065177E-4
38-39	5.149527693902462E-5	5.407004078597584E-4	0.0	0.027391196058482843	3.3042802702540797E-4
40-41	5.149527693902462E-5	6.007782309552872E-4	0.0	0.06868611689050233	3.433018462601641E-4
42-43	5.149527693902462E-5	6.436909617378077E-4	0.0	0.0806373124134343	3.4759311933841615E-4
44-45	5.149527693902462E-5	7.46681515615857E-4	0.0	0.0973174908686	3.5617566549492026E-4
46-47	5.149527693902462E-5	8.196331579461418E-4	0.0	0.11589441202435315	3.8621457704268464E-4
48-49	5.149527693902462E-5	8.28215704102646E-4	0.0	0.13379760330682072	3.947971231991887E-4
50-51	5.149527693902462E-5	9.354975310589471E-4	0.0	0.1512502109160718	3.990883962774408E-4
52-53	5.149527693902462E-5	9.354975310589471E-4	0.0	0.18322877789520609	4.20544761668701E-4
54-55	5.149527693902462E-5	9.397888041371992E-4	0.0	0.26151447266175826	4.3770985398170923E-4
56-57	5.578655001727666E-5	9.526626233719554E-4	0.0	0.3476231582499639	4.4200112705996127E-4
58-59	6.0077823095528713E-5	9.526626233719554E-4	0.0	0.441911010325318	4.462924001382133E-4
60-61	6.0077823095528713E-5	9.612451695284595E-4	0.0	0.46682614181764936	4.548749462947174E-4
62-63	6.0077823095528713E-5	9.869928079979718E-4	0.0	0.48279396894182525	4.6345749245122153E-4
64-65	6.0077823095528713E-5	0.0011328960926585414	0.0	0.4939941916760631	4.6345749245122153E-4
66-67	6.0077823095528713E-5	0.0011371873657367935	0.0	0.5009117238782055	4.6345749245122153E-4
68-69	6.866036925203282E-5	0.0011543524580498016	0.0	0.5080824411919647	4.6345749245122153E-4
70-71	7.724291540853693E-5	0.00117151755036281	0.0	0.5140387282245784	4.934964039989859E-4
72-73	7.724291540853693E-5	0.001184391369597566	0.0	0.5185231085913519	5.321178617032544E-4
74-75	7.724291540853693E-5	0.0012144302811453304	0.0	0.5219432532347187	5.321178617032544E-4
76-77	7.724291540853693E-5	0.001270216831162607	0.0	0.5250372611241385	5.364091347815063E-4
78-79	7.724291540853693E-5	0.0012959644696321194	0.0	0.5278909577211761	5.449916809380105E-4
80-81	7.724291540853693E-5	0.0012959644696321194	0.0	0.5291912134638864	5.621567732510187E-4
82-83	7.724291540853693E-5	0.0013002557427103714	0.0	0.529761952783294	5.836131386422789E-4
84-85	7.724291540853693E-5	0.001330294654258136	0.0	0.5301567499064932	5.836131386422789E-4
86-87	7.724291540853693E-5	0.0013560422927276481	0.0	0.5302811968257625	6.007782309552872E-4
88-89	7.724291540853693E-5	0.001424702661979681	0.0	0.5303498571950145	6.093607771117912E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14840	0.0	50.59643	1
GTATCAA	27930	0.0	39.004116	1
TCAACGC	33265	0.0	32.357265	4
ATCAACG	33770	0.0	31.847689	3
CAACGCA	34390	0.0	31.235664	5
TATCAAC	34835	0.0	31.048449	2
AACGCAG	35520	0.0	30.270947	6
ACGCAGA	40905	0.0	26.27528	7
CGCAGAG	41355	0.0	25.957346	8
GTGGTAT	7465	0.0	22.791027	1
TGGTATC	7055	0.0	22.639067	2
GCAGAGT	48305	0.0	22.285522	9
GAGTACT	28420	0.0	20.90786	12-13
GTACATG	34290	0.0	20.150381	1
TACATGG	34635	0.0	19.636383	2
CAGAGTA	47170	0.0	19.162182	10-11
ACATGGG	34460	0.0	19.005604	3
AGAGTAC	43765	0.0	18.958378	10-11
GTACTTT	31885	0.0	18.574873	14-15
AGTACTT	31165	0.0	17.382349	12-13
>>END_MODULE