FastQCFastQC Report
Fri 27 May 2016
SRR2139690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11166378
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149400.051.760051
GTATCAA292200.039.5496861
TCAACGC348700.032.7344134
ATCAACG350050.032.5590253
CAACGCA358450.031.7840295
TATCAAC363550.031.5984172
AACGCAG373000.030.5619476
CTTATAC151800.029.4657271
ACGCAGA422200.026.9699467
CGCAGAG425200.026.7380248
TATACAC195000.025.0111733
TTATACA203700.022.8450362
GCAGAGT500250.022.7008749
GTGGTAT75150.022.3198471
TGGTATC71400.021.8054942
GAGTACT297300.020.53006612-13
GTACATG358350.019.527011
TACATGG352500.019.5216462
CAGAGTA485700.019.51359710-11
ACACATC239200.019.030546
Produced by FastQC (version 0.11.5)