##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11166378
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94012391484508	33.0	31.0	33.0	25.0	34.0
2	31.893442708101052	33.0	32.0	33.0	28.0	34.0
3	31.657069373793366	33.0	31.0	33.0	28.0	34.0
4	32.24716510582035	33.0	33.0	34.0	30.0	34.0
5	32.273835526613915	33.0	33.0	34.0	31.0	34.0
6	35.60406409311954	38.0	36.0	38.0	29.0	38.0
7	36.28736336885604	38.0	37.0	38.0	33.0	38.0
8	36.43964318599997	38.0	37.0	38.0	34.0	38.0
9	36.63682771620305	38.0	38.0	38.0	34.0	38.0
10-11	36.70155004604	38.0	38.0	38.0	34.0	38.0
12-13	36.74137983686384	38.0	38.0	38.0	34.0	38.0
14-15	36.78653472056919	38.0	38.0	38.0	35.0	38.0
16-17	36.77141222516379	38.0	38.0	38.0	35.0	38.0
18-19	36.7726310626418	38.0	38.0	38.0	35.0	38.0
20-21	36.80113712667821	38.0	38.0	38.0	35.0	38.0
22-23	36.7753990180003	38.0	38.0	38.0	35.0	38.0
24-25	36.78148280530411	38.0	38.0	38.0	35.0	38.0
26-27	36.74507194537348	38.0	38.0	38.0	34.5	38.0
28-29	36.66591904092421	38.0	38.0	38.0	34.0	38.0
30-31	36.693973803744925	38.0	38.0	38.0	34.0	38.0
32-33	36.686547442074314	38.0	38.0	38.0	34.0	38.0
34-35	36.66774530081903	38.0	38.0	38.0	34.0	38.0
36-37	36.64004248895293	38.0	38.0	38.0	34.0	38.0
38-39	36.60475894121504	38.0	38.0	38.0	34.0	38.0
40-41	36.628789833465376	38.0	38.0	38.0	34.0	38.0
42-43	36.604107047461454	38.0	38.0	38.0	34.0	38.0
44-45	36.53981392136143	38.0	38.0	38.0	34.0	38.0
46-47	36.424471947360715	38.0	38.0	38.0	34.0	38.0
48-49	36.380295776659906	38.0	38.0	38.0	34.0	38.0
50-51	36.38688506585619	38.0	38.0	38.0	34.0	38.0
52-53	36.39243992596504	38.0	38.0	38.0	34.0	38.0
54-55	36.31132181157593	38.0	38.0	38.0	33.0	38.0
56-57	36.32941757350521	38.0	38.0	38.0	33.0	38.0
58-59	36.267135990347356	38.0	38.0	38.0	33.0	38.0
60-61	36.278448287266045	38.0	38.0	38.0	33.0	38.0
62-63	36.29310887518666	38.0	38.0	38.0	33.0	38.0
64-65	36.28021371664456	38.0	38.0	38.0	33.0	38.0
66-67	36.25952441422588	38.0	37.5	38.0	33.0	38.0
68-69	36.237306172311776	38.0	37.0	38.0	33.0	38.0
70-71	36.233109394165325	38.0	37.0	38.0	33.0	38.0
72-73	36.22043635465886	38.0	37.0	38.0	33.0	38.0
74-75	36.18423839236728	38.0	37.0	38.0	33.0	38.0
76-77	36.17721382263039	38.0	37.0	38.0	33.0	38.0
78-79	36.16741862234572	38.0	37.0	38.0	33.0	38.0
80-81	36.17601586530098	38.0	37.0	38.0	33.0	38.0
82-83	36.14346949527473	38.0	37.0	38.0	33.0	38.0
84-85	36.11352736567151	38.0	37.0	38.0	33.0	38.0
86-87	36.11661344039769	38.0	37.0	38.0	33.0	38.0
88-89	36.1239790041802	38.0	37.0	38.0	33.0	38.0
90-91	36.074594346005554	38.0	37.0	38.0	33.0	38.0
92-93	36.098157436518115	38.0	37.0	38.0	33.0	38.0
94-95	36.050782333409494	38.0	37.0	38.0	33.0	38.0
96-97	36.07888799569064	38.0	37.0	38.0	33.0	38.0
98-99	36.126333171325186	38.0	37.0	38.0	33.0	38.0
100-101	35.5866772227614	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	30.0
19	59.0
20	156.0
21	608.0
22	2593.0
23	7716.0
24	17398.0
25	32969.0
26	54114.0
27	81421.0
28	114853.0
29	153626.0
30	197463.0
31	248076.0
32	312800.0
33	402560.0
34	551408.0
35	848697.0
36	1722292.0
37	6417537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.525538182569136	20.878954661932454	14.193662439154398	25.401844716344012
2	13.895696527557996	20.798301830727922	43.202119792111645	22.10388184960244
3	17.79528688711774	26.503365728797647	30.125928031452993	25.575419352631624
4	12.288962454969731	18.874768523866916	38.8951905443287	29.941078476834655
5	13.437998067634235	37.30088719998552	35.558406570688796	13.702708161691449
6	29.138857738829905	38.44725657684166	18.794115692662384	13.619769991666054
7	25.911309455415203	33.04393079952674	22.90933730658796	18.135422438470094
8	23.741924194219468	37.32237973674185	21.178156426372098	17.757539642666583
9	24.975976990927588	17.416990540710696	21.324166170982213	36.282866297379506
10-11	23.801992911219735	27.209516819151208	28.796750387636887	20.191739881992174
12-13	24.805465120381918	24.518044257502297	28.947139349930655	21.729351272185127
14-15	21.935805862921708	25.89855457158982	26.652003899563493	25.513635665924976
16-17	21.45162468976064	29.201814590192093	27.322928706157	22.02363201389027
18-19	21.453720266320914	28.037932264159426	29.255762253436163	21.252585216083496
20-21	22.21498802783119	27.103706838521273	29.20747972513783	21.473825408509708
22-23	21.84250613987647	27.290365475522645	29.089456868294228	21.77767151630666
24-25	21.66770123745415	27.41274000026872	29.32684375293912	21.592715009338008
26-27	21.563454979076297	27.487921086672625	29.32660982075162	21.622014113499453
28-29	21.26071982481817	27.300185745545136	29.387547856330787	22.051546573305902
30-31	21.589308341047207	27.113367718001047	29.48671179132869	21.810612149623047
32-33	21.366139360805807	27.364469377634183	29.446703239459925	21.822688022100085
34-35	21.523487479066354	27.616649644549994	29.468657140440325	21.391205735943323
36-37	21.59027695087299	27.39788565321296	29.157416391933204	21.854421003980846
38-39	21.4723299691538	27.353779041620164	29.405570916773506	21.768320072452532
40-41	21.736117956208176	27.221264582651084	29.379728372406817	21.66288908873393
42-43	21.87484360289167	27.222433439925307	29.18401608879807	21.71870686838495
44-45	21.679809633754704	27.531707544519623	29.03388963325686	21.754593188468814
46-47	21.966897389554337	27.290185407641825	28.59926908233652	22.143648120467315
48-49	21.848693304960292	27.37630843432533	28.632446032991982	22.142552227722398
50-51	21.648125031383906	27.540224043211314	28.73929171610674	22.07235920929804
52-53	21.81202227351521	27.57153246119041	28.81927085017954	21.79717441511483
54-55	21.81028012689587	27.59029771975172	28.50880541570805	22.09061673764436
56-57	22.007529248509844	27.79735086396396	28.214064570786146	21.98105531674005
58-59	21.6345002821934	28.0730822034477	28.62758831005009	21.66482920430881
60-61	21.926325250813782	28.32901037371783	28.15449895522704	21.590165420241345
62-63	21.589636797098553	28.590710708788702	28.12922108619266	21.69043140792008
64-65	21.563993316435663	28.526512654753734	28.1109618659819	21.798532162828703
66-67	21.68290379196291	28.519274945847293	27.957545032683306	21.84027622950649
68-69	21.726055829425444	28.272360724617773	28.118329476854132	21.88325396910265
70-71	21.758646019635762	27.847804163933752	28.271563005380703	22.121986811049783
72-73	21.909958734988564	27.69092695819419	28.295845511877634	22.103268794939616
74-75	21.805660785368445	27.759364550998058	28.21227472725441	22.22269993637909
76-77	21.898542759290017	27.74939244578572	28.156032373887562	22.196032421036705
78-79	21.886512495587453	27.78100223485993	28.105783983310822	22.22670128624179
80-81	21.86387854381454	27.832517083180086	28.063907350713496	22.23969702229188
82-83	21.92581514341108	27.788297634797605	28.15380073326065	22.132086488530668
84-85	22.127974418020802	27.78037193041757	28.020119263051278	22.071534388510347
86-87	21.893913245532108	27.852400546840105	28.09868726792007	22.15499893970772
88-89	21.987126448317333	27.795130563109545	28.12716509862443	22.090577889948694
90-91	21.995218971100524	27.776169771055347	28.14369896637533	22.084912291468797
92-93	21.9915151411869	27.751909274154247	28.157861654967675	22.09871392969118
94-95	21.884383743628305	27.878479739249943	28.143893553798012	22.09324296332374
96-97	21.88772653193161	27.908409472872926	28.1404218527738	22.063442142421668
98-99	22.14216455510673	28.44739480902038	28.210377263122723	21.200063372750165
100-101	22.785263612225577	30.987872741350646	25.562246573113462	20.66461707331031
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3340.0
1	3111.0
2	3398.0
3	4201.0
4	5364.0
5	7708.0
6	9863.5
7	12289.5
8	15177.0
9	16587.5
10	15982.0
11	14915.5
12	15808.5
13	19660.0
14	28068.0
15	38563.5
16	46675.5
17	51566.0
18	51905.5
19	48156.5
20	43312.5
21	39104.5
22	38094.0
23	42098.5
24	51285.0
25	65745.0
26	85498.5
27	107945.0
28	131808.0
29	157778.0
30	183100.0
31	209278.5
32	238578.0
33	269773.5
34	301535.5
35	330440.0
36	360054.0
37	386259.0
38	403431.0
39	417209.0
40	433555.5
41	447757.5
42	454815.5
43	466031.5
44	497889.5
45	515788.0
46	501744.5
47	488645.0
48	474232.0
49	456897.0
50	441019.5
51	421745.5
52	399911.5
53	376293.0
54	350094.0
55	321750.0
56	292046.5
57	260494.0
58	229778.0
59	200377.0
60	167099.0
61	133411.5
62	103614.5
63	78665.0
64	59149.5
65	43486.5
66	31127.5
67	22894.0
68	17157.5
69	12385.0
70	8820.0
71	6318.5
72	4473.5
73	3049.0
74	2018.0
75	1306.5
76	825.5
77	480.0
78	299.5
79	192.5
80	131.0
81	87.5
82	57.5
83	41.0
84	30.5
85	22.0
86	13.5
87	9.5
88	8.0
89	7.0
90	5.5
91	3.0
92	1.5
93	1.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0626881877006134
6	0.0
7	0.0034478503235337364
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.4793932679736106E-5
28-29	1.7924345549656105E-5
30-31	3.588369681200715E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	9.288150998956894E-6
46-47	4.6854895589253495E-6
48-49	0.0
50-51	4.7641600245144614E-6
52-53	8.11010898210886E-4
54-55	1.4979612023216467E-4
56-57	0.0031107606028741674
58-59	0.008050105899854425
60-61	0.003235436159056416
62-63	0.005772902593969208
64-65	0.0069609324085913304
66-67	0.005094312139486537
68-69	0.0038042384151172623
70-71	0.005426507631319013
72-73	0.003324658359790319
74-75	0.003131583623792138
76-77	0.0026520689647228216
78-79	8.117507743290637E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.0151724287289737E-4
88-89	0.0
90-91	2.5956198971915337E-4
92-93	0.0
94-95	1.653813093486333E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	160.0
20-21	624.0
22-23	1261.0
24-25	1526.0
26-27	3303.0
28-29	7381.0
30-31	21260.0
32-33	27629.0
34-35	37617.0
36-37	58945.0
38-39	68299.0
40-41	69996.0
42-43	79145.0
44-45	93739.0
46-47	94356.0
48-49	86137.0
50-51	78692.0
52-53	70237.0
54-55	75630.0
56-57	77624.0
58-59	83153.0
60-61	87243.0
62-63	85709.0
64-65	98261.0
66-67	107183.0
68-69	104698.0
70-71	120581.0
72-73	84639.0
74-75	86413.0
76-77	92158.0
78-79	95013.0
80-81	95823.0
82-83	91137.0
84-85	91765.0
86-87	95976.0
88-89	99455.0
90-91	101677.0
92-93	100191.0
94-95	108523.0
96-97	167048.0
98-99	919695.0
100-101	7296476.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.79732723415671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.07195826266296	74.63992033923516
2	7.436682871886958	12.463482963043196
3	1.8999271736919534	4.776264572847934
4	0.7148663382664283	2.39615553905581
5	0.3407582433763551	1.427731501397242
6	0.17618173229816686	0.885813496444205
7	0.10415174956718998	0.610934676834423
8	0.06433604531155389	0.4312950913539052
9	0.05062494585573284	0.381801163867588
>10	0.13792774956033263	1.7428083519839477
>50	0.0018840663411036104	0.1047637231370215
>100	6.468312293753645E-4	0.09217419204400103
>500	3.599445866433207E-5	0.022802199665886116
>1k	1.7995493261795748E-5	0.024052189089700228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.955455385801914E-6
2	0.0	1.791091077160383E-5	0.0	8.955455385801914E-6	8.955455385801914E-6
3	0.0	1.791091077160383E-5	0.0	8.955455385801914E-6	8.955455385801914E-6
4	0.0	6.26881877006134E-5	0.0	8.955455385801914E-6	8.955455385801914E-6
5	8.955455385801914E-6	6.26881877006134E-5	0.0	8.955455385801914E-6	8.955455385801914E-6
6	8.955455385801914E-6	6.26881877006134E-5	0.0	8.955455385801914E-6	8.955455385801914E-6
7	8.955455385801914E-6	6.26881877006134E-5	0.0	8.955455385801914E-6	8.955455385801914E-6
8	8.955455385801914E-6	6.26881877006134E-5	0.0	8.955455385801914E-6	8.955455385801914E-6
9	8.955455385801914E-6	9.851000924382104E-5	0.0	1.791091077160383E-5	1.791091077160383E-5
10-11	3.582182154320766E-5	1.0746546462962296E-4	0.0	1.791091077160383E-5	1.791091077160383E-5
12-13	3.582182154320766E-5	1.0746546462962296E-4	0.0	2.686636615740574E-5	1.791091077160383E-5
14-15	3.582182154320766E-5	1.343318307870287E-4	0.0	3.13440938503067E-5	1.791091077160383E-5
16-17	4.029954923610861E-5	1.3880955847992967E-4	0.0	3.582182154320766E-5	1.791091077160383E-5
18-19	4.4777276929009565E-5	1.5224274155863253E-4	0.0	5.373273231481148E-5	1.791091077160383E-5
20-21	4.4777276929009565E-5	1.6119819694443443E-4	0.0	6.26881877006134E-5	1.791091077160383E-5
22-23	4.4777276929009565E-5	1.8358683540893922E-4	0.0	8.955455385801913E-5	2.6866366157405743E-5
24-25	4.925500462191052E-5	1.970200184876421E-4	0.0	1.6119819694443443E-4	3.582182154320766E-5
26-27	5.373273231481148E-5	2.0597547387344402E-4	0.0	2.820968446527603E-4	3.582182154320766E-5
28-29	5.373273231481148E-5	2.0597547387344402E-4	0.0	6.447927877777377E-4	3.582182154320766E-5
30-31	5.373273231481148E-5	2.3284184003084975E-4	0.0	0.0020104997341125297	4.029954923610861E-5
32-33	5.373273231481148E-5	2.3284184003084975E-4	0.0	0.005534471428425582	6.26881877006134E-5
34-35	6.26881877006134E-5	2.6418593388115644E-4	0.0	0.010007721393633637	7.164364308641531E-5
36-37	8.059909847221722E-5	2.8657457234566126E-4	0.0	0.01723029616228288	7.164364308641531E-5
38-39	8.059909847221722E-5	2.8657457234566126E-4	0.0	0.029315683205422563	7.164364308641531E-5
40-41	8.955455385801913E-5	3.2239639388886886E-4	0.0	0.06088366344037431	8.955455385801913E-5
42-43	8.955455385801913E-5	3.3582957696757175E-4	0.0	0.07263769863423933	8.955455385801913E-5
44-45	8.955455385801913E-5	3.940400369752842E-4	0.0	0.08856945376558092	9.403228155092008E-5
46-47	8.955455385801913E-5	4.3881731390429376E-4	0.0	0.10618035678176038	1.1194319232252392E-4
48-49	8.955455385801913E-5	4.4329504159719473E-4	0.0	0.12258675104854949	1.253763754012268E-4
50-51	8.955455385801913E-5	4.880723185262043E-4	0.0	0.13825432024600992	1.253763754012268E-4
52-53	1.1642092001542487E-4	4.970277739120062E-4	0.0	0.16253255979691894	1.2985410309412774E-4
54-55	1.1642092001542487E-4	5.015055016049072E-4	0.0	0.21364582141138336	1.3880955847992967E-4
56-57	1.1642092001542487E-4	5.10460956990709E-4	0.0	0.26991742532806967	1.4328728617283063E-4
58-59	1.1642092001542487E-4	5.10460956990709E-4	0.0	0.3320100752455273	1.477650138657316E-4
60-61	1.1642092001542487E-4	5.10460956990709E-4	0.0	0.35603756204563375	1.5224274155863253E-4
62-63	1.1642092001542487E-4	5.238941400694119E-4	0.0	0.36864236550115	1.6119819694443443E-4
64-65	1.1642092001542487E-4	5.821046000771243E-4	0.0	0.38021281385960604	1.6119819694443443E-4
66-67	1.1642092001542487E-4	5.821046000771243E-4	0.0	0.3868129844789421	1.6119819694443443E-4
68-69	1.253763754012268E-4	5.865823277700253E-4	0.0	0.3929698600566809	1.6119819694443443E-4
70-71	1.253763754012268E-4	5.910600554629263E-4	0.0	0.3987550842359089	1.6119819694443443E-4
72-73	1.253763754012268E-4	5.910600554629263E-4	0.0	0.4031701237411093	2.0597547387344402E-4
74-75	1.253763754012268E-4	5.910600554629263E-4	0.0	0.4069627590969963	2.0597547387344402E-4
76-77	1.253763754012268E-4	6.17926421620332E-4	0.0	0.4103479212328295	2.0597547387344402E-4
78-79	1.253763754012268E-4	6.358373323919359E-4	0.0	0.41335247651476603	2.0597547387344402E-4
80-81	1.253763754012268E-4	6.358373323919359E-4	0.0	0.41441817570567646	2.2388638464504782E-4
82-83	1.253763754012268E-4	6.358373323919359E-4	0.0	0.4149420698457459	2.3284184003084975E-4
84-85	1.253763754012268E-4	6.358373323919359E-4	0.0	0.41539432034272883	2.3284184003084975E-4
86-87	1.253763754012268E-4	6.447927877777377E-4	0.0	0.4156226844550668	2.3284184003084975E-4
88-89	1.253763754012268E-4	6.537482431635397E-4	0.0	0.41568537264276745	2.3284184003084975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14940	0.0	51.76005	1
GTATCAA	29220	0.0	39.549686	1
TCAACGC	34870	0.0	32.734413	4
ATCAACG	35005	0.0	32.559025	3
CAACGCA	35845	0.0	31.784029	5
TATCAAC	36355	0.0	31.598417	2
AACGCAG	37300	0.0	30.561947	6
CTTATAC	15180	0.0	29.465727	1
ACGCAGA	42220	0.0	26.969946	7
CGCAGAG	42520	0.0	26.738024	8
TATACAC	19500	0.0	25.011173	3
TTATACA	20370	0.0	22.845036	2
GCAGAGT	50025	0.0	22.700874	9
GTGGTAT	7515	0.0	22.319847	1
TGGTATC	7140	0.0	21.805494	2
GAGTACT	29730	0.0	20.530066	12-13
GTACATG	35835	0.0	19.52701	1
TACATGG	35250	0.0	19.521646	2
CAGAGTA	48570	0.0	19.513597	10-11
ACACATC	23920	0.0	19.03054	6
>>END_MODULE