FastQCFastQC Report
Fri 27 May 2016
SRR2139689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11987907
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168350.052.17911
GTATCAA319300.038.9292681
TCAACGC382900.031.9785614
ATCAACG384850.031.794113
CAACGCA393250.031.0930275
TATCAAC398800.031.049792
AACGCAG408300.029.9689376
ACGCAGA463300.026.383417
CGCAGAG473600.025.7993288
TGGTATC75400.025.5208642
GTGGTAT80750.024.8451921
CTTATAC89100.023.5337141
GCAGAGT545800.022.4418139
GAGTACT336550.020.55466512-13
CAGAGTA536800.019.4889310-11
TATACAC140800.019.0598563
GTACATG354350.019.0186861
AGAGTAC499600.018.89050510-11
TACATGG353950.018.698872
GTACTTT374250.018.46147514-15
Produced by FastQC (version 0.11.5)