FastQCFastQC Report
Fri 27 May 2016
SRR2139688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11378314
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158650.049.878611
GTATCAA298150.039.33311
TCAACGC358150.032.1400384
ATCAACG360350.031.8718193
CTTATAC93800.031.6245651
TATCAAC369000.031.4176562
CAACGCA368550.031.1978915
AACGCAG380000.030.3533556
ACGCAGA434250.026.4718067
CGCAGAG438600.026.2275858
TATACAC137300.024.8490493
GTGGTAT77450.023.9518571
TGGTATC74650.023.5179542
GCAGAGT507850.022.7277959
TTATACA144050.022.063652
GAGTACT310100.020.26196312-13
ACATCTC153000.019.5166598
TATGCCG86600.019.50751942-43
CAGAGTA500050.019.4311410-11
ACACATC156350.019.1271386
Produced by FastQC (version 0.11.5)