##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11378314
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90250040559612	33.0	31.0	33.0	25.0	34.0
2	31.872788885945667	33.0	32.0	33.0	28.0	34.0
3	31.65136961416252	33.0	31.0	33.0	28.0	34.0
4	32.245570477313244	33.0	33.0	34.0	30.0	34.0
5	32.27378344454196	33.0	33.0	34.0	31.0	34.0
6	35.61269332169951	38.0	36.0	38.0	29.0	38.0
7	36.28677359404917	38.0	37.0	38.0	33.0	38.0
8	36.43844026452425	38.0	37.0	38.0	34.0	38.0
9	36.63412074934828	38.0	38.0	38.0	34.0	38.0
10-11	36.701366081125904	38.0	38.0	38.0	34.0	38.0
12-13	36.74615681198462	38.0	38.0	38.0	34.0	38.0
14-15	36.79021953516136	38.0	38.0	38.0	35.0	38.0
16-17	36.77438968550174	38.0	38.0	38.0	35.0	38.0
18-19	36.77720244844711	38.0	38.0	38.0	35.0	38.0
20-21	36.80386598456942	38.0	38.0	38.0	35.0	38.0
22-23	36.777050427297056	38.0	38.0	38.0	35.0	38.0
24-25	36.78380318369233	38.0	38.0	38.0	35.0	38.0
26-27	36.74832034900027	38.0	38.0	38.0	34.5	38.0
28-29	36.6744772333433	38.0	38.0	38.0	34.0	38.0
30-31	36.69299888484637	38.0	38.0	38.0	34.0	38.0
32-33	36.689525285931026	38.0	38.0	38.0	34.0	38.0
34-35	36.677535983273515	38.0	38.0	38.0	34.0	38.0
36-37	36.65055506931944	38.0	38.0	38.0	34.0	38.0
38-39	36.628919633246696	38.0	38.0	38.0	34.0	38.0
40-41	36.638389546551664	38.0	38.0	38.0	34.0	38.0
42-43	36.61074692487248	38.0	38.0	38.0	34.0	38.0
44-45	36.54904603228465	38.0	38.0	38.0	34.0	38.0
46-47	36.43190385556265	38.0	38.0	38.0	34.0	38.0
48-49	36.38512838573063	38.0	38.0	38.0	34.0	38.0
50-51	36.395686376045354	38.0	38.0	38.0	34.0	38.0
52-53	36.40180533033184	38.0	38.0	38.0	34.0	38.0
54-55	36.32616308770174	38.0	38.0	38.0	33.0	38.0
56-57	36.34627342553227	38.0	38.0	38.0	34.0	38.0
58-59	36.29160810782438	38.0	38.0	38.0	33.0	38.0
60-61	36.30553998128937	38.0	38.0	38.0	33.0	38.0
62-63	36.322966793296814	38.0	38.0	38.0	33.0	38.0
64-65	36.31366394422034	38.0	38.0	38.0	33.0	38.0
66-67	36.29409486121904	38.0	38.0	38.0	33.0	38.0
68-69	36.2753855372524	38.0	37.5	38.0	33.0	38.0
70-71	36.2751557396176	38.0	37.5	38.0	33.0	38.0
72-73	36.26653333415855	38.0	37.5	38.0	33.0	38.0
74-75	36.2295156110241	38.0	37.0	38.0	33.0	38.0
76-77	36.22025538222888	38.0	37.0	38.0	33.0	38.0
78-79	36.20998348258386	38.0	37.0	38.0	33.0	38.0
80-81	36.217302211105455	38.0	37.0	38.0	33.0	38.0
82-83	36.18799510544133	38.0	37.0	38.0	33.0	38.0
84-85	36.15595352090932	38.0	37.0	38.0	33.0	38.0
86-87	36.15829187878951	38.0	37.0	38.0	33.0	38.0
88-89	36.16557643633826	38.0	37.0	38.0	33.0	38.0
90-91	36.1127733018467	38.0	37.0	38.0	33.0	38.0
92-93	36.13079245211255	38.0	37.0	38.0	33.0	38.0
94-95	36.08466359278686	38.0	37.0	38.0	33.0	38.0
96-97	36.114502770803256	38.0	37.0	38.0	33.0	38.0
98-99	36.164423393269864	38.0	37.0	38.0	33.0	38.0
100-101	35.63232044227169	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	20.0
19	54.0
20	109.0
21	569.0
22	2441.0
23	7315.0
24	16768.0
25	32116.0
26	53154.0
27	80642.0
28	114487.0
29	154713.0
30	198989.0
31	252624.0
32	318241.0
33	412159.0
34	563518.0
35	866953.0
36	1748564.0
37	6554875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.041828692721964	20.559372856119104	14.13092484528024	25.267873605878695
2	14.202227148943155	20.673704381861846	42.61773756639165	22.506330902803352
3	17.96712588525857	26.066181685617046	29.874628174262018	26.09206425486236
4	12.526064933697558	18.57827969943526	38.66209879600792	30.233556570859264
5	13.757744789429227	36.95579814366889	35.36298004943122	13.923477017470653
6	29.73513474843461	38.07566744950087	18.567979403626936	13.62121839843759
7	26.410737504256055	32.82856593923983	22.68086223889546	18.079834317608658
8	24.15463310293599	36.96332338868483	20.875078680373914	18.006964828005273
9	25.166136213150736	17.310156847490763	21.206648014811332	36.31705892454717
10-11	24.001029502261932	26.98566325380017	28.643813134353646	20.36949410958425
12-13	25.018912075055066	24.359947927251955	28.842985284505062	21.778154713187917
14-15	22.134074521058217	25.728411959803537	26.585880825577497	25.551632693560748
16-17	21.640130514942722	29.02418143848025	27.210744931103147	22.124943115473876
18-19	21.62358588451681	27.877482551457096	29.23824215081426	21.260689413211832
20-21	22.352208395600297	26.9230852048505	29.26607940058132	21.458626998967883
22-23	22.071167452962342	27.01657032931426	29.107318010210427	21.80494420751297
24-25	21.93809869023141	27.141589308489404	29.211090315831278	21.709221685447908
26-27	21.947088919040283	27.221685793117818	29.144166133839803	21.687059154002092
28-29	21.79133929688546	27.156970598783523	29.228633933756505	21.82305617057451
30-31	22.048611936527056	27.08994876698266	29.172748331339125	21.68869096515116
32-33	21.65559128830501	27.14475847580577	29.304131987701805	21.895518248187415
34-35	21.802635067795148	27.177934082760718	29.149899728874235	21.8695311205699
36-37	21.826784548317416	27.163466936067838	29.242937560278676	21.76681095533607
38-39	21.70663584001112	27.19874024954369	29.30510693330736	21.789516977137822
40-41	21.99035279325778	27.044024479825257	29.182872200405413	21.782750526511553
42-43	21.984545861460376	27.128926585820572	28.963995475802275	21.922532076916777
44-45	21.955702244289412	27.339265906296774	28.79235859688553	21.912673252528283
46-47	22.16822178327847	27.214945927742605	28.438798036258333	22.178034252720586
48-49	22.044170924558117	27.345844838876747	28.400754291182967	22.20922994538217
50-51	21.91758076584028	27.47552376196368	28.450848782901755	22.15604668929429
52-53	22.131741292075006	27.38106387040785	28.488330199188706	21.998864638328442
54-55	22.0605271251341	27.36311500618931	28.319775280750154	22.256582587926435
56-57	22.214122736017234	27.544697120999416	28.12557381173607	22.115606331247275
58-59	22.013614509814897	27.752988475114698	28.329892874397224	21.903504140673185
60-61	22.19415490513707	27.89521671880858	28.063817407612223	21.846810968442124
62-63	21.99370791698266	28.087409538078433	28.047254361779217	21.87162818315969
64-65	21.986855763129455	28.017103640473767	28.01579121246376	21.98024938393302
66-67	22.056063428759863	27.991784491084363	27.895538133738196	22.05661394641757
68-69	22.041027115857435	27.855823849727617	28.036713433205257	22.06643560120969
70-71	22.114896930667747	27.49140658645135	28.15232041906824	22.241376063812663
72-73	22.20020377416751	27.44487336197214	28.089139184504575	22.265783679355774
74-75	22.065648279232253	27.573309489679886	28.065734485959904	22.295307745127953
76-77	22.160077359308843	27.528806734533795	28.035768432489267	22.275347473668095
78-79	22.132952059251313	27.54876785837223	27.985147577152315	22.33313250522415
80-81	22.163835949481584	27.57768697341185	27.959663161775588	22.298813915330975
82-83	22.219610486901015	27.56249795149263	28.00393656171668	22.213954999889676
84-85	22.324425163201166	27.540136400311887	27.92163164778772	22.213806788699227
86-87	22.14570575115949	27.588967367771822	28.017325178993346	22.24800170207534
88-89	22.217605902613062	27.56546997835503	27.939700765406876	22.277223353625036
90-91	22.273045858924352	27.586385901914483	27.920415292441596	22.220152946719562
92-93	22.282225200886487	27.58329237309801	27.934788411516504	22.199694014498995
94-95	22.1692650544855	27.67554546646096	27.908091659995787	22.24709781905775
96-97	22.20803124484359	27.67288717770626	27.904512497862356	22.2145690795878
98-99	22.48476383648623	28.250951589876085	27.94627598847367	21.318008585164016
100-101	23.077920955056893	30.771792944029276	25.29431002066615	20.855976080247682
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2947.0
1	2809.5
2	3393.0
3	4398.5
4	5557.5
5	8036.5
6	10520.0
7	13620.0
8	17398.5
9	19145.5
10	18057.5
11	16486.0
12	17415.5
13	21584.0
14	30555.5
15	41681.5
16	50232.5
17	55253.5
18	55612.0
19	51530.5
20	46273.5
21	42278.5
22	41604.0
23	46071.0
24	55961.0
25	71503.0
26	91914.0
27	114046.0
28	136691.0
29	160996.0
30	183873.5
31	207252.0
32	233481.5
33	260856.0
34	287950.0
35	311103.0
36	337266.0
37	363277.0
38	381943.0
39	397368.5
40	413039.5
41	429297.5
42	438567.0
43	446086.0
44	457930.0
45	477395.0
46	487533.5
47	481842.0
48	475562.5
49	465521.5
50	454071.0
51	441524.5
52	422573.0
53	399950.0
54	377204.0
55	351204.0
56	322716.5
57	292047.5
58	260210.0
59	226312.5
60	189341.5
61	153066.0
62	119571.0
63	91497.0
64	68853.0
65	50839.5
66	37042.5
67	27354.5
68	20382.5
69	14752.0
70	10560.5
71	7484.0
72	5225.0
73	3447.5
74	2202.0
75	1372.5
76	844.5
77	509.0
78	326.5
79	216.5
80	141.5
81	96.5
82	71.5
83	48.5
84	36.5
85	34.0
86	27.5
87	19.0
88	11.5
89	5.0
90	2.5
91	3.0
92	3.0
93	2.0
94	1.5
95	2.5
96	3.0
97	2.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06116020352400189
6	0.0
7	0.00351545932024727
8	0.0
9	0.0
10-11	0.0
12-13	1.3182972450927265E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.23150531651183E-5
28-29	1.3193373337986784E-5
30-31	3.081551906518746E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.548519006759918E-6
46-47	0.0
48-49	0.0
50-51	4.661452241883503E-6
52-53	7.182216493974261E-4
54-55	1.749057104587233E-4
56-57	0.0028863906632597097
58-59	0.007109738940563446
60-61	0.003285577933884398
62-63	0.005749360085006654
64-65	0.006217671181826431
66-67	0.004651905776194036
68-69	0.0037312468534821
70-71	0.004995744506389315
72-73	0.0031797165574077887
74-75	0.002918156937448945
76-77	0.002377236787349147
78-79	7.3518472802865E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	7.097435117977934E-5
88-89	5.516048086921444E-6
90-91	1.672764823763074E-4
92-93	0.0
94-95	8.551790727874836E-5
96-97	5.775472055320706E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	125.0
20-21	594.0
22-23	1328.0
24-25	1582.0
26-27	3631.0
28-29	8342.0
30-31	20052.0
32-33	25982.0
34-35	36202.0
36-37	56029.0
38-39	66956.0
40-41	66374.0
42-43	76401.0
44-45	91480.0
46-47	92937.0
48-49	84666.0
50-51	76302.0
52-53	73053.0
54-55	78286.0
56-57	82122.0
58-59	82387.0
60-61	80295.0
62-63	79527.0
64-65	89124.0
66-67	97789.0
68-69	96835.0
70-71	108274.0
72-73	83039.0
74-75	84581.0
76-77	89441.0
78-79	92804.0
80-81	93230.0
82-83	89002.0
84-85	88788.0
86-87	92239.0
88-89	96661.0
90-91	98449.0
92-93	97373.0
94-95	106530.0
96-97	170298.0
98-99	947559.0
100-101	7571645.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.99587908371298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74659972165931	76.28091138116544
2	7.216269484285502	12.267063370436365
3	1.6863487893817901	4.299980933857812
4	0.6174881250961163	2.0993578406519253
5	0.28611064163215955	1.2159112750365282
6	0.14927651512847348	0.761273317793867
7	0.08598354840198809	0.5115773098214773
8	0.05195812009193628	0.3532980874201003
9	0.03592336358066636	0.2748004080464319
>10	0.12045260358777754	1.5983810420882452
>50	0.002733694680905444	0.15839445243464217
>100	7.866106140879928E-4	0.11214117325937399
>500	1.719560604503821E-5	0.011253978521749284
>1k	5.158625321981328E-5	0.05565542946598148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6365944901854527E-5	0.0	8.788648300618175E-6	8.788648300618175E-6
3	0.0	2.6365944901854527E-5	0.0	8.788648300618175E-6	8.788648300618175E-6
4	0.0	7.909783470556358E-5	0.0	8.788648300618175E-6	8.788648300618175E-6
5	0.0	7.909783470556358E-5	0.0	8.788648300618175E-6	8.788648300618175E-6
6	0.0	9.667513130679994E-5	0.0	8.788648300618175E-6	2.6365944901854527E-5
7	0.0	9.667513130679994E-5	0.0	8.788648300618175E-6	2.6365944901854527E-5
8	0.0	1.0546377960741811E-4	0.0	8.788648300618175E-6	2.6365944901854527E-5
9	0.0	1.5819566941112717E-4	0.0	8.788648300618175E-6	2.6365944901854527E-5
10-11	4.3943241503090876E-6	1.6258999356143626E-4	0.0	3.9548917352781786E-5	2.6365944901854527E-5
12-13	8.788648300618175E-6	1.757729660123635E-4	0.0	4.394324150309088E-5	2.6365944901854527E-5
14-15	8.788648300618175E-6	2.680537731688544E-4	0.0	4.833756565339997E-5	2.6365944901854527E-5
16-17	1.757729660123635E-5	2.724480973191635E-4	0.0	5.2731889803709054E-5	2.6365944901854527E-5
18-19	1.757729660123635E-5	2.900253939203998E-4	0.0	6.152053810432723E-5	2.6365944901854527E-5
20-21	1.757729660123635E-5	2.9881404222101797E-4	0.0	7.470351055525449E-5	2.6365944901854527E-5
22-23	1.757729660123635E-5	3.4275728372410883E-4	0.0	8.349215885587266E-5	2.6365944901854527E-5
24-25	1.757729660123635E-5	3.603345803253452E-4	0.0	8.788648300618175E-5	2.6365944901854527E-5
26-27	1.757729660123635E-5	3.7791187692658157E-4	0.0	1.1864675205834537E-4	2.6365944901854527E-5
28-29	1.757729660123635E-5	3.8230620107689066E-4	0.0	3.647289044756543E-4	2.6365944901854527E-5
30-31	1.757729660123635E-5	4.2185511842967243E-4	0.0	0.0010853980651263449	2.6365944901854527E-5
32-33	1.757729660123635E-5	4.306437667302906E-4	0.0	0.002522342062277416	3.51545932024727E-5
34-35	1.757729660123635E-5	4.877699806843088E-4	0.0	0.004447056040112797	3.51545932024727E-5
36-37	1.757729660123635E-5	5.580791670892542E-4	0.0	0.007329732682715559	3.51545932024727E-5
38-39	1.757729660123635E-5	5.888394361414178E-4	0.0	0.011618593053417229	3.51545932024727E-5
40-41	1.757729660123635E-5	6.767259191475996E-4	0.0	0.016215056114640535	3.51545932024727E-5
42-43	1.757729660123635E-5	6.98697539899145E-4	0.0	0.020565437023446532	3.51545932024727E-5
44-45	1.757729660123635E-5	7.690067263040905E-4	0.0	0.025605726823851056	3.51545932024727E-5
46-47	1.757729660123635E-5	8.085556436568722E-4	0.0	0.031151363901541124	3.9548917352781786E-5
48-49	1.757729660123635E-5	8.173442919574903E-4	0.0	0.03687277394524356	6.152053810432723E-5
50-51	2.197162075154544E-5	9.403853681661449E-4	0.0	0.04252826912669136	6.152053810432723E-5
52-53	2.6365944901854527E-5	9.49174016466763E-4	0.0	0.04785418999686597	6.591486225463632E-5
54-55	2.6365944901854527E-5	9.53568340617072E-4	0.0	0.05375137300658077	7.470351055525449E-5
56-57	2.6365944901854527E-5	9.755399613686175E-4	0.0	0.05937610791897639	7.909783470556358E-5
58-59	2.6365944901854527E-5	9.799342855189267E-4	0.0	0.06523374201133841	7.909783470556358E-5
60-61	3.0760269052163615E-5	0.001001905906270472	0.0	0.07074861881997632	7.909783470556358E-5
62-63	3.51545932024727E-5	0.0010150888787213994	0.0	0.07593831564149135	7.909783470556358E-5
64-65	3.9548917352781786E-5	0.0011688902239822174	0.0	0.08118074435281009	7.909783470556358E-5
66-67	4.394324150309088E-5	0.0011732845481325265	0.0	0.08647590495393254	7.909783470556358E-5
68-69	4.394324150309088E-5	0.0011952561688840719	0.0	0.09185455771391086	7.909783470556358E-5
70-71	4.394324150309088E-5	0.0012172277896356173	0.0	0.0969959169697725	7.909783470556358E-5
72-73	4.394324150309088E-5	0.0012260164379362356	0.0	0.10075745844243708	1.0546377960741811E-4
74-75	4.394324150309088E-5	0.0012348050862368539	0.0	0.10395213209971178	1.0546377960741811E-4
76-77	4.394324150309088E-5	0.0012787483277399447	0.0	0.10679965414911208	1.098581037577272E-4
78-79	4.394324150309088E-5	0.0013226915692430355	0.0	0.10948458620495093	1.1425242790803629E-4
80-81	4.394324150309088E-5	0.0013314802175436536	0.0	0.11065347642893314	1.1864675205834537E-4
82-83	4.394324150309088E-5	0.0013402688658442719	0.0	0.11114124640961745	1.3182972450927265E-4
84-85	4.394324150309088E-5	0.0013534518382951992	0.0	0.1115982561212496	1.3182972450927265E-4
86-87	4.394324150309088E-5	0.0013710291348964353	0.0	0.11177402908726197	1.406183728098908E-4
88-89	4.394324150309088E-5	0.0014281553488504537	0.0	0.11182676097706568	1.406183728098908E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15865	0.0	49.87861	1
GTATCAA	29815	0.0	39.3331	1
TCAACGC	35815	0.0	32.140038	4
ATCAACG	36035	0.0	31.871819	3
CTTATAC	9380	0.0	31.624565	1
TATCAAC	36900	0.0	31.417656	2
CAACGCA	36855	0.0	31.197891	5
AACGCAG	38000	0.0	30.353355	6
ACGCAGA	43425	0.0	26.471806	7
CGCAGAG	43860	0.0	26.227585	8
TATACAC	13730	0.0	24.849049	3
GTGGTAT	7745	0.0	23.951857	1
TGGTATC	7465	0.0	23.517954	2
GCAGAGT	50785	0.0	22.727795	9
TTATACA	14405	0.0	22.06365	2
GAGTACT	31010	0.0	20.261963	12-13
ACATCTC	15300	0.0	19.516659	8
TATGCCG	8660	0.0	19.507519	42-43
CAGAGTA	50005	0.0	19.43114	10-11
ACACATC	15635	0.0	19.127138	6
>>END_MODULE