FastQCFastQC Report
Fri 27 May 2016
SRR2139687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11220790
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149850.050.8673131
GTATCAA284650.039.303271
TCAACGC344900.031.9493834
ATCAACG347350.031.7116033
TATCAAC358900.031.027812
CAACGCA355500.030.98465
AACGCAG366450.030.08566
ACGCAGA417550.026.2797437
CGCAGAG424550.025.8252038
TGGTATC69450.024.8595122
GTGGTAT75300.024.0172821
GCAGAGT492850.022.2638059
GAGTACT301100.020.1620512-13
CTTATAC76150.020.0082761
GTACATG336150.019.5171011
TACATGG337000.019.1861022
CAGAGTA485550.019.03641910-11
AGAGTAC452800.018.95110510-11
ACATGGG340550.018.2383173
GTACTTT336600.017.97840914-15
Produced by FastQC (version 0.11.5)