##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11220790
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88157527232931	33.0	31.0	33.0	25.0	34.0
2	31.860951323391667	33.0	32.0	33.0	28.0	34.0
3	31.654449642137497	33.0	31.0	33.0	28.0	34.0
4	32.2439466383383	33.0	33.0	34.0	30.0	34.0
5	32.27672151426058	33.0	33.0	34.0	31.0	34.0
6	35.62580914534538	38.0	36.0	38.0	29.0	38.0
7	36.293345744818325	38.0	37.0	38.0	33.0	38.0
8	36.44717029727853	38.0	37.0	38.0	34.0	38.0
9	36.6389956500389	38.0	38.0	38.0	34.0	38.0
10-11	36.706218902590635	38.0	38.0	38.0	34.0	38.0
12-13	36.748344100549076	38.0	38.0	38.0	34.0	38.0
14-15	36.79033771240706	38.0	38.0	38.0	35.0	38.0
16-17	36.77729420121044	38.0	38.0	38.0	35.0	38.0
18-19	36.77889328647983	38.0	38.0	38.0	35.0	38.0
20-21	36.804006456001645	38.0	38.0	38.0	35.0	38.0
22-23	36.779104779083745	38.0	38.0	38.0	35.0	38.0
24-25	36.78671188779175	38.0	38.0	38.0	35.0	38.0
26-27	36.75064653184022	38.0	38.0	38.0	35.0	38.0
28-29	36.67643749438336	38.0	38.0	38.0	34.0	38.0
30-31	36.70138022700449	38.0	38.0	38.0	34.0	38.0
32-33	36.697174756733645	38.0	38.0	38.0	34.0	38.0
34-35	36.6857533570997	38.0	38.0	38.0	34.0	38.0
36-37	36.659994805553524	38.0	38.0	38.0	34.0	38.0
38-39	36.645379299825535	38.0	38.0	38.0	34.0	38.0
40-41	36.643011833417994	38.0	38.0	38.0	34.0	38.0
42-43	36.61371484313446	38.0	38.0	38.0	34.0	38.0
44-45	36.5541801532287	38.0	38.0	38.0	34.0	38.0
46-47	36.4385442652368	38.0	38.0	38.0	34.0	38.0
48-49	36.39086888261101	38.0	38.0	38.0	34.0	38.0
50-51	36.40362441786818	38.0	38.0	38.0	34.0	38.0
52-53	36.408035966760295	38.0	38.0	38.0	34.0	38.0
54-55	36.33438672064128	38.0	38.0	38.0	33.0	38.0
56-57	36.354240935440984	38.0	38.0	38.0	34.0	38.0
58-59	36.29987761403706	38.0	38.0	38.0	33.5	38.0
60-61	36.314894918658425	38.0	38.0	38.0	33.0	38.0
62-63	36.332517997149615	38.0	38.0	38.0	33.0	38.0
64-65	36.32303580174164	38.0	38.0	38.0	33.5	38.0
66-67	36.30406880148134	38.0	38.0	38.0	33.0	38.0
68-69	36.28555542471689	38.0	38.0	38.0	33.0	38.0
70-71	36.28585855905663	38.0	38.0	38.0	33.0	38.0
72-73	36.277986081796406	38.0	37.5	38.0	33.0	38.0
74-75	36.2400690419619	38.0	37.0	38.0	33.0	38.0
76-77	36.2312189680929	38.0	37.0	38.0	33.0	38.0
78-79	36.21757168986442	38.0	37.0	38.0	33.0	38.0
80-81	36.22313641810378	38.0	37.0	38.0	33.0	38.0
82-83	36.19348795668169	38.0	37.0	38.0	33.0	38.0
84-85	36.16023438152648	38.0	37.0	38.0	33.0	38.0
86-87	36.16040560144606	38.0	37.0	38.0	33.0	38.0
88-89	36.16636444494125	38.0	37.0	38.0	33.0	38.0
90-91	36.112317406450494	38.0	37.0	38.0	33.0	38.0
92-93	36.13077472619052	38.0	37.0	38.0	33.0	38.0
94-95	36.08645654574108	38.0	37.0	38.0	33.0	38.0
96-97	36.11398373957367	38.0	37.0	38.0	33.0	38.0
98-99	36.16347730693552	38.0	37.0	38.0	33.0	38.0
100-101	35.62277323815334	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	19.0
19	44.0
20	117.0
21	544.0
22	2400.0
23	7370.0
24	16751.0
25	31615.0
26	52763.0
27	79192.0
28	112335.0
29	151169.0
30	195172.0
31	246387.0
32	313619.0
33	405855.0
34	554551.0
35	854528.0
36	1719477.0
37	6476877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.04072797013401	20.62768307757297	14.175374461156478	25.15621449113654
2	14.164680026985621	20.872790596740515	42.627711596064096	22.33481778020977
3	17.919620632771846	26.194528192756483	29.901236900432142	25.98461427403953
4	12.494877811633584	18.607076685331425	38.928141423197474	29.969904079837516
5	13.725691627982298	37.0763446429374	35.348540980460555	13.849422748619741
6	29.542946619623038	38.212059935173905	18.789987157766966	13.455006287436088
7	26.343992872963568	32.927794194345786	22.90110345717593	17.827109475514717
8	24.061122256097832	37.06608001753887	20.99702427369196	17.875773452671336
9	25.247839055895348	17.385166285083315	21.399143910544623	35.96785074847671
10-11	23.884093722456264	27.14486680527842	28.746768275673993	20.224271196591328
12-13	24.88828437606819	24.563142370686126	28.990999251879735	21.557574001365946
14-15	22.0570788687784	25.920683837768998	26.79614358703799	25.22609370641461
16-17	21.45763355343073	29.13096136724776	27.43677138597193	21.974633693349578
18-19	21.453966253712974	28.062284384611065	29.448608342193378	21.035141019482587
20-21	22.236232194434884	27.13617095873573	29.442304180559663	21.185292666269717
22-23	21.956035566276245	27.11036394273645	29.273763391676994	21.659837099310312
24-25	21.717531282989192	27.248706772489385	29.401704502962346	21.632057441559077
26-27	21.685639733145436	27.399152246471964	29.342599416421134	21.57260860396147
28-29	21.54587903595365	27.36751034011472	29.449553779031657	21.637056844899973
30-31	21.862214114885077	27.224572147701938	29.26364015231731	21.649573585095673
32-33	21.722359583110066	27.33282744135554	29.319710953712224	21.62510202182217
34-35	21.815452390160242	27.446480475734088	29.28415919829166	21.45390793581401
36-37	21.72587906361506	27.327107820648422	29.220859526867876	21.72615358886864
38-39	21.59123076282644	27.447304216567638	29.33665574079068	21.624809279815242
40-41	21.854102535436127	27.304795853746384	29.26433004114717	21.576771569670324
42-43	21.802634623216505	27.337640990434082	29.073135490386605	21.78658889596281
44-45	21.802394282990917	27.49683608022749	28.942466940424623	21.75830269635697
46-47	21.975549349629237	27.474638982787546	28.595746519198613	21.954065148384604
48-49	21.84179685840217	27.57986125766923	28.601913703834665	21.976428180093937
50-51	21.82572476653504	27.689182515924976	28.51665423239616	21.968438485143828
52-53	21.988629107518847	27.64100673685361	28.45591895010663	21.91444520552092
54-55	21.964101301802245	27.604937031394556	28.37743343016329	22.05352823663991
56-57	21.997352371732816	27.7224733785092	28.3259825750242	21.954191674733785
58-59	21.906777239530186	27.846331237500905	28.411482260270287	21.835409262698622
60-61	22.01818318867368	27.87392725464966	28.331336071048874	21.77655348562778
62-63	21.910484414807048	27.933404744015718	28.312020901810182	21.844089939367052
64-65	21.88974221050731	27.904845374759084	28.266685455154878	21.93872695957873
66-67	21.957314734746987	27.9358865824477	28.145064170279305	21.961734512526007
68-69	21.896884673585088	27.861030401420717	28.249380964414428	21.992703960579767
70-71	21.952661912135003	27.563025737524498	28.36191629192627	22.12239605841423
72-73	22.03860526738414	27.56541743563278	28.293413577973116	22.102563719009964
74-75	21.939838427576927	27.692033613938527	28.225475165234997	22.142652793249546
76-77	22.031380846625513	27.646209101815217	28.18522944127243	22.13718061028684
78-79	21.960880041787025	27.69494359782091	28.151007915492883	22.19316844489918
80-81	22.007143808199626	27.724100188543538	28.13593628579376	22.132819717463075
82-83	22.045032145614957	27.68546503956939	28.218775585300666	22.050727229514987
84-85	22.155889913831107	27.667198858786367	28.072727052585755	22.104184174796767
86-87	21.993704804006157	27.73540830939187	28.15212315546977	22.1187637311322
88-89	22.06542761506671	27.70950531767792	28.093962817664153	22.131104249591214
90-91	22.123584722876423	27.7505850882192	28.080433365852436	22.045396823051934
92-93	22.15615302541595	27.728511131815242	28.05914955065827	22.056186292110542
94-95	22.042050452823485	27.827751750232295	28.037276204188654	22.09292159275557
96-97	22.044414352844793	27.84470763205723	28.067476838611725	22.04340117648625
98-99	22.31433256439548	28.391376624431096	28.094913068007333	21.199377743166085
100-101	22.897357167946662	30.898035814822943	25.500739312972392	20.703867704258
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2954.0
1	2740.0
2	3334.0
3	4464.0
4	5566.0
5	7837.0
6	10193.0
7	13332.5
8	17151.5
9	19026.0
10	18091.5
11	16763.5
12	17681.0
13	21286.5
14	29603.5
15	40013.5
16	48191.5
17	52858.0
18	52852.0
19	49302.0
20	44728.5
21	41196.5
22	41230.5
23	46428.5
24	57063.5
25	73333.5
26	94148.5
27	116830.0
28	140154.5
29	165080.0
30	190113.5
31	215559.5
32	242809.0
33	270406.5
34	296861.0
35	322766.0
36	350612.5
37	373960.0
38	391594.0
39	406515.5
40	420358.5
41	433558.5
42	441428.5
43	447693.5
44	454603.5
45	464558.0
46	469195.0
47	466222.5
48	463147.0
49	454017.0
50	441177.0
51	426173.0
52	406687.0
53	385635.5
54	363597.5
55	337438.5
56	308050.0
57	277989.0
58	247707.0
59	214961.0
60	178791.5
61	144204.5
62	113037.0
63	86912.5
64	66108.0
65	48705.0
66	35312.0
67	26245.5
68	19441.5
69	13881.5
70	9967.0
71	7080.5
72	4786.0
73	3072.5
74	1934.5
75	1219.0
76	777.5
77	483.5
78	300.0
79	195.0
80	139.0
81	91.5
82	59.5
83	40.0
84	27.5
85	19.5
86	15.0
87	15.5
88	12.5
89	7.5
90	4.5
91	2.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06200989413401374
6	0.0
7	0.003725227902848195
8	0.0
9	0.0
10-11	0.0
12-13	4.456014237856693E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.903347443978587E-5
28-29	3.121610404916126E-5
30-31	4.463953707728702E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.8459944942291214E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	9.104868444661562E-4
54-55	1.8727615997052755E-4
56-57	0.0030783176277826103
58-59	0.00805655339377066
60-61	0.0032029301251027117
62-63	0.005579681547487231
64-65	0.006565205490931866
66-67	0.005085513053346578
68-69	0.003682674671093347
70-71	0.005314199061459973
72-73	0.0033188317525254672
74-75	0.0028660520949766698
76-77	0.0026335546700301422
78-79	5.874361296717193E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.8914124698756035E-5
88-89	0.0
90-91	1.818187809937101E-4
92-93	0.0
94-95	8.138445656866156E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	115.0
20-21	547.0
22-23	1224.0
24-25	1485.0
26-27	3539.0
28-29	8225.0
30-31	20192.0
32-33	26380.0
34-35	35881.0
36-37	56164.0
38-39	66881.0
40-41	66560.0
42-43	77020.0
44-45	92490.0
46-47	92684.0
48-49	86102.0
50-51	78003.0
52-53	75124.0
54-55	80969.0
56-57	83757.0
58-59	83798.0
60-61	82445.0
62-63	80505.0
64-65	87636.0
66-67	93500.0
68-69	93656.0
70-71	97835.0
72-73	84109.0
74-75	86551.0
76-77	91220.0
78-79	94320.0
80-81	94669.0
82-83	89684.0
84-85	90364.0
86-87	94068.0
88-89	97989.0
90-91	99844.0
92-93	98268.0
94-95	106818.0
96-97	168494.0
98-99	929235.0
100-101	7422440.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.15409616704262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.04794249923796	76.67951155224434
2	6.907522539245459	11.764076771658445
3	1.662985815648792	4.248301622105552
4	0.6276562344148575	2.137899973808265
5	0.28067839032839026	1.1950457321017236
6	0.15352103072467926	0.7843766768395705
7	0.09388916170136732	0.5596532693192874
8	0.05849812200603594	0.39850837655147026
9	0.03478236283943706	0.2665674603131754
>10	0.1294041955748331	1.689785923376115
>50	0.002331861849672411	0.1294919629896338
>100	7.359181180160379E-4	0.09741559449028744
>500	1.7289459936862295E-5	0.012926515973747464
>1k	3.4578850593006376E-5	0.036438568228379715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.912028475713385E-6	0.0	0.0	8.912028475713385E-6
3	0.0	8.912028475713385E-6	0.0	1.782405695142677E-5	8.912028475713385E-6
4	0.0	1.782405695142677E-5	0.0	1.782405695142677E-5	8.912028475713385E-6
5	0.0	1.782405695142677E-5	0.0	1.782405695142677E-5	8.912028475713385E-6
6	4.456014237856693E-5	1.782405695142677E-5	0.0	1.782405695142677E-5	1.782405695142677E-5
7	4.456014237856693E-5	1.782405695142677E-5	0.0	3.564811390285354E-5	2.6736085427140157E-5
8	5.3472170854280314E-5	1.782405695142677E-5	0.0	3.564811390285354E-5	2.6736085427140157E-5
9	5.3472170854280314E-5	7.129622780570708E-5	0.0	3.564811390285354E-5	2.6736085427140157E-5
10-11	5.3472170854280314E-5	7.129622780570708E-5	0.0	7.129622780570708E-5	2.6736085427140157E-5
12-13	5.3472170854280314E-5	7.129622780570708E-5	0.0	7.129622780570708E-5	3.1192099664996845E-5
14-15	6.23841993299937E-5	1.3368042713570079E-4	0.0	7.129622780570708E-5	7.12962278057071E-5
16-17	9.803231323284724E-5	1.5150448408712757E-4	0.0	7.129622780570708E-5	1.247683986599874E-4
18-19	9.803231323284724E-5	1.782405695142677E-4	0.0	7.129622780570708E-5	1.247683986599874E-4
20-21	1.0694434170856063E-4	1.9606462646569448E-4	0.0	8.020825628142047E-5	1.247683986599874E-4
22-23	1.1585637018427402E-4	2.0497665494140785E-4	0.0	1.2031238442213071E-4	1.3368042713570079E-4
24-25	1.292244128978441E-4	2.361687546064047E-4	0.0	1.5596049832498427E-4	1.6041651256284094E-4
26-27	1.3813644137355746E-4	2.5844882579568817E-4	0.0	2.0497665494140788E-4	1.6041651256284094E-4
28-29	1.4259245561141416E-4	2.5844882579568817E-4	0.0	4.678814949749527E-4	1.782405695142677E-4
30-31	1.4259245561141416E-4	2.9409693969854173E-4	0.0	0.0013590843425462912	2.0052064070355118E-4
32-33	1.4259245561141416E-4	2.9409693969854173E-4	0.0	0.0028964092546068503	2.1388868341712126E-4
34-35	1.4259245561141416E-4	3.3420106783925196E-4	0.0	0.004857055519263796	2.2280071189283463E-4
36-37	1.4259245561141416E-4	3.787612102178189E-4	0.0	0.007873777158292777	2.3171274036854804E-4
38-39	1.4259245561141416E-4	4.188653383585291E-4	0.0	0.012494663922950167	2.3616875460640474E-4
40-41	1.4704846984927086E-4	4.4560142378566927E-4	0.0	0.017387367556116813	2.539928115578315E-4
42-43	1.5150448408712757E-4	4.678814949749527E-4	0.0	0.02251623994388987	2.6736085427140157E-4
44-45	1.5150448408712757E-4	5.525457654942298E-4	0.0	0.028424914823287843	2.6736085427140157E-4
46-47	1.5150448408712757E-4	6.193859790620803E-4	0.0	0.034538566357627226	2.6736085427140157E-4
48-49	1.5150448408712757E-4	6.327540217756504E-4	0.0	0.04042496116583592	2.76272882747115E-4
50-51	1.7378455527641102E-4	7.664344489113511E-4	0.0	0.04665892508459743	2.851849112228283E-4
52-53	1.7824056951426772E-4	7.798024916249212E-4	0.0	0.0526611762629904	2.9409693969854173E-4
54-55	1.7824056951426772E-4	7.931705343384914E-4	0.0	0.05881493192547049	3.119209966499685E-4
56-57	1.7824056951426772E-4	7.931705343384914E-4	0.0	0.06509791200084843	3.208330251256819E-4
58-59	1.7824056951426772E-4	7.97626548576348E-4	0.0	0.0716749890159249	3.386570820771087E-4
60-61	1.9606462646569448E-4	8.199066197656315E-4	0.0	0.07787776083502142	3.564811390285354E-4
62-63	2.1388868341712126E-4	8.377306767170583E-4	0.0	0.08402260446902579	3.653931675042488E-4
64-65	2.1388868341712126E-4	9.624990753770457E-4	0.0	0.08951687002430311	3.653931675042488E-4
66-67	2.1388868341712126E-4	9.669550896149023E-4	0.0	0.09567953771525892	3.743051959799622E-4
68-69	2.1388868341712126E-4	9.803231323284724E-4	0.0	0.10214521437438898	3.876732386935323E-4
70-71	2.1388868341712126E-4	0.001002603203517756	0.0	0.10755481565914699	3.9212925293138895E-4
72-73	2.6736085427140157E-4	0.0010204272604691826	0.0	0.11171673295730514	4.0104128140710236E-4
74-75	2.6736085427140157E-4	0.0010694434170856063	0.0	0.11510775979231408	4.0104128140710236E-4
76-77	2.6736085427140157E-4	0.0011541076876048833	0.0	0.11818240961643521	4.0549729564495903E-4
78-79	2.6736085427140157E-4	0.0011852997872698803	0.0	0.12094068242966849	4.0995330988281576E-4
80-81	2.6736085427140157E-4	0.001189755801507737	0.0	0.12216163033084124	4.1440932412067244E-4
82-83	2.6736085427140157E-4	0.001203123844221307	0.0	0.12274091218176261	4.277773668342425E-4
84-85	2.6736085427140157E-4	0.0012120358726970205	0.0	0.12320879367673757	4.322333810720992E-4
86-87	2.6736085427140157E-4	0.0012209479011727339	0.0	0.12335138613234897	4.366893953099559E-4
88-89	2.76272882747115E-4	0.0012744200720270142	0.0	0.1234315943886304	4.366893953099559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14985	0.0	50.867313	1
GTATCAA	28465	0.0	39.30327	1
TCAACGC	34490	0.0	31.949383	4
ATCAACG	34735	0.0	31.711603	3
TATCAAC	35890	0.0	31.02781	2
CAACGCA	35550	0.0	30.9846	5
AACGCAG	36645	0.0	30.0856	6
ACGCAGA	41755	0.0	26.279743	7
CGCAGAG	42455	0.0	25.825203	8
TGGTATC	6945	0.0	24.859512	2
GTGGTAT	7530	0.0	24.017282	1
GCAGAGT	49285	0.0	22.263805	9
GAGTACT	30110	0.0	20.16205	12-13
CTTATAC	7615	0.0	20.008276	1
GTACATG	33615	0.0	19.517101	1
TACATGG	33700	0.0	19.186102	2
CAGAGTA	48555	0.0	19.036419	10-11
AGAGTAC	45280	0.0	18.951105	10-11
ACATGGG	34055	0.0	18.238317	3
GTACTTT	33660	0.0	17.978409	14-15
>>END_MODULE