FastQCFastQC Report
Fri 27 May 2016
SRR2139686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11543720
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157000.051.612781
GTATCAA297800.039.7766231
TCAACGC364000.032.1859554
ATCAACG366100.031.9067943
TATCAAC377550.031.214172
CAACGCA375300.031.170755
AACGCAG384800.030.4701466
ACGCAGA440550.026.6240757
CGCAGAG448650.026.1422658
TGGTATC73600.024.5119232
GTGGTAT78250.023.939921
GCAGAGT520550.022.539729
GAGTACT318900.020.5448312-13
CAGAGTA508600.019.3335110-11
AGAGTAC470600.019.20893310-11
GTACATG341200.019.0956781
TACATGG340200.018.6304132
GTACTTT352950.018.48952514-15
AGTACTT342150.018.04948412-13
ACATGGG339950.018.0205213
Produced by FastQC (version 0.11.5)