##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11543720
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.880323673824382	33.0	31.0	33.0	25.0	34.0
2	31.861920420800228	33.0	32.0	33.0	28.0	34.0
3	31.635662853915374	33.0	31.0	33.0	28.0	34.0
4	32.23472173614745	33.0	33.0	34.0	30.0	34.0
5	32.27041958744668	33.0	33.0	34.0	31.0	34.0
6	35.607383148586415	38.0	36.0	38.0	29.0	38.0
7	36.286393380989836	38.0	37.0	38.0	33.0	38.0
8	36.442356536714335	38.0	37.0	38.0	34.0	38.0
9	36.633787808436104	38.0	38.0	38.0	34.0	38.0
10-11	36.702896509963864	38.0	38.0	38.0	34.0	38.0
12-13	36.7465810414667	38.0	38.0	38.0	34.0	38.0
14-15	36.79101299234562	38.0	38.0	38.0	35.0	38.0
16-17	36.77971035333498	38.0	38.0	38.0	35.0	38.0
18-19	36.781640407078484	38.0	38.0	38.0	35.0	38.0
20-21	36.806385471332376	38.0	38.0	38.0	35.0	38.0
22-23	36.783225734023034	38.0	38.0	38.0	35.0	38.0
24-25	36.79150142475966	38.0	38.0	38.0	35.0	38.0
26-27	36.7568276122404	38.0	38.0	38.0	35.0	38.0
28-29	36.680703074105566	38.0	38.0	38.0	34.0	38.0
30-31	36.70574919853608	38.0	38.0	38.0	34.0	38.0
32-33	36.705134805016016	38.0	38.0	38.0	34.0	38.0
34-35	36.69481698743365	38.0	38.0	38.0	34.0	38.0
36-37	36.66863650525899	38.0	38.0	38.0	34.0	38.0
38-39	36.66484495433362	38.0	38.0	38.0	34.0	38.0
40-41	36.6518617952466	38.0	38.0	38.0	34.0	38.0
42-43	36.6185028036917	38.0	38.0	38.0	34.0	38.0
44-45	36.560328937820415	38.0	38.0	38.0	34.0	38.0
46-47	36.445661527144736	38.0	38.0	38.0	34.0	38.0
48-49	36.40025274324812	38.0	38.0	38.0	34.0	38.0
50-51	36.41346625129506	38.0	38.0	38.0	34.0	38.0
52-53	36.420919518438865	38.0	38.0	38.0	34.0	38.0
54-55	36.348872078638934	38.0	38.0	38.0	34.0	38.0
56-57	36.368755084683926	38.0	38.0	38.0	34.0	38.0
58-59	36.31824613443397	38.0	38.0	38.0	33.5	38.0
60-61	36.33400145989614	38.0	38.0	38.0	33.0	38.0
62-63	36.35214416407065	38.0	38.0	38.0	34.0	38.0
64-65	36.34198209845928	38.0	38.0	38.0	33.5	38.0
66-67	36.32595087349431	38.0	38.0	38.0	33.0	38.0
68-69	36.308872700897034	38.0	38.0	38.0	33.0	38.0
70-71	36.309902692448375	38.0	38.0	38.0	33.0	38.0
72-73	36.302710963277235	38.0	38.0	38.0	33.0	38.0
74-75	36.26583490405741	38.0	37.5	38.0	33.0	38.0
76-77	36.259597340928295	38.0	37.0	38.0	33.0	38.0
78-79	36.25044503349008	38.0	37.0	38.0	33.0	38.0
80-81	36.254644934659495	38.0	37.0	38.0	33.0	38.0
82-83	36.224490445919116	38.0	37.0	38.0	33.0	38.0
84-85	36.19052472696052	38.0	37.0	38.0	33.0	38.0
86-87	36.192810615930824	38.0	37.0	38.0	33.0	38.0
88-89	36.19770918542628	38.0	37.0	38.0	33.0	38.0
90-91	36.14300232203917	38.0	37.0	38.0	33.0	38.0
92-93	36.16404354711425	38.0	37.0	38.0	33.0	38.0
94-95	36.11747261152448	38.0	37.0	38.0	33.0	38.0
96-97	36.1473080157849	38.0	37.0	38.0	33.0	38.0
98-99	36.19193693078183	38.0	37.0	38.0	33.0	38.0
100-101	35.66935940017572	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	33.0
20	128.0
21	538.0
22	2361.0
23	7376.0
24	16599.0
25	31534.0
26	52377.0
27	79730.0
28	113651.0
29	153196.0
30	198625.0
31	251164.0
32	320302.0
33	415286.0
34	569258.0
35	874124.0
36	1764513.0
37	6692912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12256014525647	20.677372632045824	14.201271340607708	24.998795882090004
2	14.108173101911689	20.92679829379091	42.65489807445087	22.310130529846532
3	17.966071595638148	26.342530830616127	29.795915008333534	25.895482565412188
4	12.479997782343993	18.494124944125463	39.138102795286095	29.88777447824445
5	13.706613405580903	37.08962445525677	35.3557122583826	13.848049880779723
6	29.497406381998175	38.2787610926114	18.83022976995284	13.39360275543759
7	26.220631840990833	32.99865965352667	23.1162206526323	17.664487852850193
8	24.020021275637315	37.07061501838229	21.06295024480843	17.846413461171963
9	25.200966412906755	17.45767395605576	21.562581212988537	35.778778418048944
10-11	23.864902301857633	27.164501564487008	28.799212905371924	20.17138322828343
12-13	24.90647727188069	24.616748260828963	29.073449299437147	21.4033251678532
14-15	22.0035265928141	25.954527656595967	26.93522538661714	25.10672036397279
16-17	21.403854216838248	29.12354509638141	27.561267078550067	21.911333608230276
18-19	21.36987470243561	28.13159016330957	29.578874920736126	20.919660213518693
20-21	22.19188043334052	27.189157902091182	29.563011968958598	21.0559496956097
22-23	21.939583463826306	27.080859381463736	29.440822118301448	21.538735036408514
24-25	21.645916644904645	27.244393586491338	29.50528414209782	21.604405626506193
26-27	21.639219375520867	27.389211597977166	29.41718337142958	21.55438565507239
28-29	21.591905473698702	27.4124080421635	29.472065051938873	21.52362143219893
30-31	21.84470564957214	27.24803297033737	29.374078209090708	21.53318317099978
32-33	21.644447939862122	27.422364664840092	29.326532572184394	21.606654823113388
34-35	21.782004131800946	27.422791121413738	29.318199668727164	21.477005078058156
36-37	21.765601330858573	27.32463521153411	29.284300396916972	21.625463060690343
38-39	21.60719690038147	27.45972836108726	29.378563083289933	21.554511655241342
40-41	21.846536712487776	27.336386618358638	29.25532606989853	21.561750599255056
42-43	21.731789072051537	27.46804527861318	29.036414396421804	21.763751252913483
44-45	21.832606476381365	27.501476020405807	28.901168257980952	21.76474924523188
46-47	21.916259400389084	27.554490180011808	28.627383093250458	21.90186732634865
48-49	21.866282423485245	27.66635346264798	28.592938933614715	21.874425180252064
50-51	21.832853345703846	27.79508129489059	28.46124613017018	21.910819229235383
52-53	22.01883231247862	27.723458383653217	28.343561187500228	21.914148116367937
54-55	21.96706836763774	27.653817220255167	28.374315694005553	22.004798718101544
56-57	21.93100721870231	27.749335752095906	28.390390311000623	21.929266718201163
58-59	21.922442941493316	27.825642493586184	28.338742952011884	21.91317161290861
60-61	21.953095130741847	27.764768753660057	28.440746108954112	21.841390006643984
62-63	21.945187557149	27.74793841942879	28.41499782547674	21.891876197945468
64-65	21.952002651457697	27.759953906443936	28.316224796714856	21.97181864538351
66-67	21.99713856225733	27.789654447840967	28.20753603369071	22.00567095621099
68-69	21.952086715703224	27.748853062192254	28.298041051275895	22.001019170828627
70-71	21.95336759501302	27.557165113493355	28.36017136093173	22.129295930561895
72-73	22.051009486751454	27.58451087204029	28.273474238589323	22.091005402618926
74-75	21.901069960243188	27.74552289784567	28.218082707306984	22.135324434604158
76-77	22.052071578186	27.67356040158614	28.16470891971402	22.10965910051384
78-79	21.99016484843595	27.72080085938619	28.14310311030979	22.145931181868068
80-81	22.008303393839192	27.73377945465064	28.135805806629392	22.12211134488077
82-83	22.061921965009127	27.705580608314843	28.185142989439253	22.047354437236773
84-85	22.175689487222723	27.73529295032794	28.044242679471516	22.044774882977826
86-87	22.005477633087896	27.767225611929526	28.164691621524124	22.06260513345846
88-89	22.060664762205686	27.748305616514422	28.097930380146664	22.093099241133228
90-91	22.120531178269598	27.773907174899453	28.103220816144418	22.002340830686528
92-93	22.12112799698131	27.76938450191525	28.071081046766327	22.03840645433711
94-95	22.025854328252002	27.825788814187565	28.068126755433386	22.08023010212705
96-97	22.051199442965224	27.833169361082387	28.090947182403823	22.02468401354856
98-99	22.29495766593898	28.435596731246715	28.117478796544	21.15196680627031
100-101	22.874345402716383	30.92358707692755	25.52533880521231	20.676728715143756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2812.0
1	2752.5
2	3541.5
3	4664.5
4	5880.5
5	8494.0
6	11128.5
7	14208.5
8	17774.0
9	19303.5
10	18046.5
11	16586.5
12	17761.5
13	21970.0
14	30763.5
15	41648.0
16	50174.0
17	55272.0
18	55544.0
19	51684.5
20	46964.5
21	43356.5
22	43118.5
23	48705.0
24	60065.5
25	77653.5
26	100078.5
27	123410.5
28	148584.0
29	175367.5
30	200832.0
31	227307.0
32	256193.0
33	283760.5
34	309024.5
35	333249.5
36	360724.5
37	385428.0
38	402660.5
39	415600.0
40	429199.5
41	442705.5
42	450074.0
43	457216.0
44	465403.0
45	471862.5
46	474753.0
47	474314.0
48	471330.0
49	463385.5
50	452221.0
51	437923.0
52	416955.0
53	393408.5
54	369979.0
55	342821.0
56	313510.0
57	282957.5
58	252060.5
59	219668.5
60	183797.0
61	149371.5
62	117298.0
63	89580.0
64	67631.0
65	49935.5
66	36460.0
67	27302.0
68	20803.0
69	15238.0
70	10863.5
71	7694.0
72	5402.5
73	3655.5
74	2367.0
75	1507.0
76	918.0
77	509.0
78	317.0
79	207.5
80	126.5
81	92.0
82	72.5
83	48.0
84	30.5
85	21.5
86	13.0
87	10.0
88	9.0
89	6.5
90	5.5
91	3.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06143600156621956
6	0.0
7	0.0037769453867557428
8	0.0
9	0.0
10-11	0.0
12-13	1.7325437553925425E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.232490737906309E-5
28-29	2.1673340538418662E-5
30-31	2.6034687923931502E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.794689737403241E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	8.016209609887683E-4
54-55	1.586875160904474E-4
56-57	0.003415027598456163
58-59	0.008189398063119632
60-61	0.003700259654008512
62-63	0.006509894027020445
64-65	0.0068962673661489135
66-67	0.005255325817221531
68-69	0.004269423976630438
70-71	0.005590492950318571
72-73	0.0035978926714901574
74-75	0.003218249443874821
76-77	0.0025355898204520674
78-79	4.1376304142316284E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	8.070369316240649E-5
88-89	5.436553329570877E-6
90-91	1.2642426554372604E-4
92-93	0.0
94-95	7.309322135151952E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	142.0
20-21	529.0
22-23	1227.0
24-25	1596.0
26-27	3594.0
28-29	8416.0
30-31	21244.0
32-33	27196.0
34-35	37480.0
36-37	58398.0
38-39	69347.0
40-41	68828.0
42-43	78836.0
44-45	94639.0
46-47	95239.0
48-49	88017.0
50-51	79356.0
52-53	76691.0
54-55	82077.0
56-57	85476.0
58-59	85505.0
60-61	83403.0
62-63	80067.0
64-65	85530.0
66-67	89566.0
68-69	92345.0
70-71	90999.0
72-73	86380.0
74-75	87716.0
76-77	92748.0
78-79	95631.0
80-81	95835.0
82-83	91348.0
84-85	91549.0
86-87	95052.0
88-89	99833.0
90-91	102467.0
92-93	100130.0
94-95	108990.0
96-97	171629.0
98-99	953127.0
100-101	7685542.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.16897338534139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.44345337444393	75.28363646566596
2	7.2076095548622	12.133141935902577
3	1.8452153083785585	4.659296345434181
4	0.6694720306903187	2.2539509413361554
5	0.32232463514469484	1.3564866818466847
6	0.17001443888854617	0.8585964469160277
7	0.09288984905390518	0.5472910262950567
8	0.06433113517832868	0.4331748483738837
9	0.03826905851522137	0.2898960630884766
>10	0.14237638113169754	1.8483128714337982
>50	0.0032142158542783216	0.1761021306619348
>100	7.763700769787003E-4	0.10514581639742204
>500	0.0	0.0
>1k	5.364778143820541E-5	0.05496842664786215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.662718776962713E-6
2	0.0	5.1976312661776276E-5	0.0	0.0	8.662718776962713E-6
3	0.0	5.1976312661776276E-5	0.0	0.0	8.662718776962713E-6
4	0.0	1.1261534410051526E-4	0.0	0.0	8.662718776962713E-6
5	0.0	1.1261534410051526E-4	0.0	0.0	8.662718776962713E-6
6	2.5988156330888138E-5	1.1261534410051526E-4	0.0	0.0	4.331359388481356E-5
7	2.5988156330888138E-5	1.1261534410051526E-4	0.0	1.7325437553925425E-5	4.331359388481356E-5
8	2.5988156330888138E-5	1.1261534410051526E-4	0.0	1.7325437553925425E-5	5.1976312661776276E-5
9	2.5988156330888138E-5	2.4255612575495595E-4	0.0	4.331359388481356E-5	6.063903143873899E-5
10-11	2.5988156330888138E-5	2.4255612575495595E-4	0.0	4.331359388481356E-5	6.063903143873899E-5
12-13	2.5988156330888138E-5	2.4255612575495595E-4	0.0	5.1976312661776276E-5	6.063903143873899E-5
14-15	2.5988156330888138E-5	3.6383418863243395E-4	0.0	5.1976312661776276E-5	8.229582838114577E-5
16-17	4.331359388481356E-5	3.8115962618635933E-4	0.0	6.497039082722034E-5	1.1261534410051526E-4
18-19	4.331359388481356E-5	4.1147914190572883E-4	0.0	7.796446899266441E-5	1.2994078165444068E-4
20-21	4.331359388481356E-5	4.6778681395598647E-4	0.0	8.662718776962713E-5	1.3427214104292203E-4
22-23	6.063903143873899E-5	5.457512829486509E-4	0.0	1.082839847120339E-4	1.4293485981988475E-4
24-25	6.063903143873899E-5	6.193843925528339E-4	0.0	1.6459165676229153E-4	1.5592893798532881E-4
26-27	6.063903143873899E-5	6.583666270491661E-4	0.0	2.165679694240678E-4	1.6026029737381016E-4
28-29	6.063903143873899E-5	6.583666270491661E-4	0.0	5.197631266177627E-4	1.6459165676229153E-4
30-31	6.063903143873899E-5	6.843547833800543E-4	0.0	0.0016285911300689898	1.689230161507729E-4
32-33	6.93017502157017E-5	6.886861427685357E-4	0.0	0.003945868402906515	1.9057981309317967E-4
34-35	7.363310960418305E-5	7.753133305381627E-4	0.0	0.006726601130311547	2.2089932881254917E-4
36-37	7.796446899266441E-5	8.619405183077899E-4	0.0	0.011257203050663045	2.2523068820103052E-4
38-39	7.796446899266441E-5	8.662718776962712E-4	0.0	0.01823935438489499	2.4255612575495595E-4
40-41	7.796446899266441E-5	9.269109091350102E-4	0.0	0.025407754172831636	2.468874851434373E-4
42-43	7.796446899266441E-5	9.615617842428611E-4	0.0	0.03231194103807092	2.7287564147432543E-4
44-45	7.796446899266441E-5	0.0011088280034512272	0.0	0.04041158309453105	2.9886379780521356E-4
46-47	7.796446899266441E-5	0.0012127806287747797	0.0	0.04940781654440683	3.118578759706577E-4
48-49	7.796446899266441E-5	0.0012127806287747797	0.0	0.058369399119174756	3.205205947476204E-4
50-51	7.796446899266441E-5	0.0013470527698177018	0.0	0.06728333674066939	3.205205947476204E-4
52-53	8.229582838114577E-5	0.001360046847983146	0.0	0.07602835134601324	3.3351467291306444E-4
54-55	8.662718776962713E-5	0.00137304092614859	0.0	0.08484266770157281	3.465087510785085E-4
56-57	8.662718776962713E-5	0.0013773722855370712	0.0	0.091998073411344	3.465087510785085E-4
58-59	8.662718776962713E-5	0.0013946977230909967	0.0	0.09795802392989435	3.508401104669899E-4
60-61	1.0395262532355255E-4	0.0014206858794218848	0.0	0.10351948938470441	3.7682826679787796E-4
62-63	1.0395262532355255E-4	0.0014510053951412544	0.0	0.10882540463559408	4.158105012942102E-4
64-65	1.0395262532355255E-4	0.0015852775361841764	0.0	0.11429158018385754	4.2014186068269153E-4
66-67	1.1261534410051526E-4	0.0015896088955726579	0.0	0.11994400418582571	4.244732200711729E-4
68-69	1.1261534410051526E-4	0.001654579286399878	0.0	0.12547948148430488	4.244732200711729E-4
70-71	1.1261534410051526E-4	0.001689230161507729	0.0	0.130672781391094	4.331359388481356E-4
72-73	1.386035004314034E-4	0.0016978928802846916	0.0	0.1347875728101513	4.591240951790237E-4
74-75	1.386035004314034E-4	0.0017671946305003934	0.0	0.13819635264888613	4.6778681395598647E-4
76-77	1.386035004314034E-4	0.0018408277401045763	0.0	0.14148385442474348	4.6778681395598647E-4
78-79	1.386035004314034E-4	0.0018884726933778712	0.0	0.1442732498709255	4.6778681395598647E-4
80-81	1.386035004314034E-4	0.0018884726933778712	0.0	0.14542972282765002	4.894436108983933E-4
82-83	1.386035004314034E-4	0.0018928040527663526	0.0	0.14584120196955574	5.284258453947255E-4
84-85	1.386035004314034E-4	0.0019014667715433154	0.0	0.146248349752073	5.284258453947255E-4
86-87	1.386035004314034E-4	0.001957774443593573	0.0	0.14635663373678504	5.284258453947255E-4
88-89	1.386035004314034E-4	0.002113703381578902	0.0	0.1463826218931159	5.327572047832068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15700	0.0	51.61278	1
GTATCAA	29780	0.0	39.776623	1
TCAACGC	36400	0.0	32.185955	4
ATCAACG	36610	0.0	31.906794	3
TATCAAC	37755	0.0	31.21417	2
CAACGCA	37530	0.0	31.17075	5
AACGCAG	38480	0.0	30.470146	6
ACGCAGA	44055	0.0	26.624075	7
CGCAGAG	44865	0.0	26.142265	8
TGGTATC	7360	0.0	24.511923	2
GTGGTAT	7825	0.0	23.93992	1
GCAGAGT	52055	0.0	22.53972	9
GAGTACT	31890	0.0	20.54483	12-13
CAGAGTA	50860	0.0	19.33351	10-11
AGAGTAC	47060	0.0	19.208933	10-11
GTACATG	34120	0.0	19.095678	1
TACATGG	34020	0.0	18.630413	2
GTACTTT	35295	0.0	18.489525	14-15
AGTACTT	34215	0.0	18.049484	12-13
ACATGGG	33995	0.0	18.020521	3
>>END_MODULE