FastQCFastQC Report
Fri 27 May 2016
SRR2139685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11417062
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171500.052.5433161
GTATCAA330450.039.8423541
TCAACGC401950.032.3079874
ATCAACG404000.032.154643
TATCAAC414500.031.5568872
CAACGCA412200.031.4942175
AACGCAG426750.030.493836
TGGTATC75050.027.1951182
ACGCAGA491450.026.4360037
GTGGTAT81450.026.2664971
CGCAGAG501250.025.9185818
GCAGAGT575350.022.6176599
GAGTACT331750.020.4699812-13
GTACATG434600.019.7104991
TACATGG426400.019.547672
CAGAGTA567250.019.2918410-11
ACATGGG424550.019.0886143
AGAGTAC537850.018.44212710-11
GTACTTT367750.018.32693714-15
AGTACTT359550.017.26971412-13
Produced by FastQC (version 0.11.5)