FastQCFastQC Report
Fri 27 May 2016
SRR2139684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10871120
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149550.048.9488831
GTATCAA279000.038.906781
TCAACGC331100.032.471034
ATCAACG331550.032.3225633
CAACGCA338200.031.7381785
TATCAAC348000.030.993592
AACGCAG351450.030.6213556
ACGCAGA399350.026.872697
CGCAGAG404200.026.5382048
GTGGTAT75000.024.343331
TGGTATC71850.024.1460672
GCAGAGT473950.022.6235249
GAGTACT302550.020.43525112-13
CAGAGTA459350.019.39882310-11
AGAGTAC433800.018.93175310-11
GTACATG304850.018.8327431
TACATGG296100.018.6440852
GTACTTT336900.018.28798914-15
ACATGGG302950.017.594163
AGTACTT327550.017.39058112-13
Produced by FastQC (version 0.11.5)