##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139684_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10871120
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.835073571076393	33.0	31.0	33.0	25.0	34.0
2	31.854875118663028	33.0	32.0	33.0	28.0	34.0
3	31.63485206676037	33.0	31.0	33.0	28.0	34.0
4	32.23851903023792	33.0	33.0	34.0	30.0	34.0
5	32.268154799137534	33.0	33.0	34.0	31.0	34.0
6	35.6061529998749	38.0	36.0	38.0	29.0	38.0
7	36.28701927676265	38.0	37.0	38.0	33.0	38.0
8	36.438084484395354	38.0	37.0	38.0	34.0	38.0
9	36.63193295630993	38.0	38.0	38.0	34.0	38.0
10-11	36.69972762696024	38.0	38.0	38.0	34.0	38.0
12-13	36.74466145162596	38.0	38.0	38.0	34.0	38.0
14-15	36.78789549742805	38.0	38.0	38.0	35.0	38.0
16-17	36.7747240394734	38.0	38.0	38.0	35.0	38.0
18-19	36.7769348972323	38.0	38.0	38.0	35.0	38.0
20-21	36.79974097618758	38.0	38.0	38.0	35.0	38.0
22-23	36.77405644093349	38.0	38.0	38.0	35.0	38.0
24-25	36.783248631462016	38.0	38.0	38.0	35.0	38.0
26-27	36.745915710683505	38.0	38.0	38.0	34.5	38.0
28-29	36.67101888898149	38.0	38.0	38.0	34.0	38.0
30-31	36.69260924589982	38.0	38.0	38.0	34.0	38.0
32-33	36.689014771092246	38.0	38.0	38.0	34.0	38.0
34-35	36.679072987675184	38.0	38.0	38.0	34.0	38.0
36-37	36.65268746014958	38.0	38.0	38.0	34.0	38.0
38-39	36.64512147292483	38.0	38.0	38.0	34.0	38.0
40-41	36.63088816258815	38.0	38.0	38.0	34.0	38.0
42-43	36.59940305618403	38.0	38.0	38.0	34.0	38.0
44-45	36.540221318329486	38.0	38.0	38.0	34.0	38.0
46-47	36.425485661190855	38.0	38.0	38.0	34.0	38.0
48-49	36.380348211951556	38.0	38.0	38.0	34.0	38.0
50-51	36.394672386394134	38.0	38.0	38.0	34.0	38.0
52-53	36.39891315647799	38.0	38.0	38.0	34.0	38.0
54-55	36.32639941057212	38.0	38.0	38.0	33.0	38.0
56-57	36.346552685330444	38.0	38.0	38.0	34.0	38.0
58-59	36.29376894258211	38.0	38.0	38.0	33.5	38.0
60-61	36.307134917597836	38.0	38.0	38.0	33.0	38.0
62-63	36.32418513565502	38.0	38.0	38.0	33.0	38.0
64-65	36.31225432272604	38.0	38.0	38.0	33.0	38.0
66-67	36.29473372036784	38.0	38.0	38.0	33.0	38.0
68-69	36.2769993431338	38.0	37.5	38.0	33.0	38.0
70-71	36.27989677300985	38.0	37.0	38.0	33.0	38.0
72-73	36.27255334249548	38.0	37.0	38.0	33.0	38.0
74-75	36.23523057006804	38.0	37.0	38.0	33.0	38.0
76-77	36.22432664617486	38.0	37.0	38.0	33.0	38.0
78-79	36.21100025937621	38.0	37.0	38.0	33.0	38.0
80-81	36.21792676625604	38.0	37.0	38.0	33.0	38.0
82-83	36.18436603271431	38.0	37.0	38.0	33.0	38.0
84-85	36.152620346328476	38.0	37.0	38.0	33.0	38.0
86-87	36.15217410731617	38.0	37.0	38.0	33.0	38.0
88-89	36.15847222734029	38.0	37.0	38.0	33.0	38.0
90-91	36.10342210166985	38.0	37.0	38.0	33.0	38.0
92-93	36.119639911932836	38.0	37.0	38.0	33.0	38.0
94-95	36.072871605899316	38.0	37.0	38.0	33.0	38.0
96-97	36.10133362108049	38.0	37.0	38.0	33.0	38.0
98-99	36.14759845982491	38.0	37.0	38.0	33.0	38.0
100-101	35.60155668931084	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	23.0
19	42.0
20	104.0
21	558.0
22	2525.0
23	7242.0
24	16356.0
25	31052.0
26	51179.0
27	76938.0
28	110353.0
29	147512.0
30	189162.0
31	239955.0
32	304177.0
33	395637.0
34	540587.0
35	831954.0
36	1671266.0
37	6254492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.866665072228066	20.937244736512888	14.122767479339753	25.07332271191929
2	13.972433383128877	21.037804752408213	43.064458859804695	21.925303004658215
3	17.946117787311703	26.39736292120775	29.833788974825044	25.822730316655505
4	12.297527761628976	18.702681968371245	39.573650185077526	29.426140084922253
5	13.479604030677505	37.20555720774417	35.65481941578427	13.660019345794058
6	29.12290545960306	38.6866394630912	19.013385925277248	13.177069152028494
7	25.941269521202965	33.04562226835624	23.310364037640845	17.702744172799946
8	23.544280626099244	37.567794302702936	21.253237936845515	17.634687134352305
9	24.905336340689825	17.521368543443547	21.795077232152714	35.778217883713914
10-11	23.645903090022003	27.39979873278926	28.996915681180962	19.95738249600777
12-13	24.661633268847485	24.753247572765968	29.282528321141548	21.302590837245
14-15	21.74779139591873	26.16987026175776	27.178253022687638	24.90408531963588
16-17	21.157295660428733	29.303944763740997	27.827841105608254	21.71091847022202
18-19	21.051823547159813	28.348909771946225	29.887463297249962	20.711803383644003
20-21	21.869892452396016	27.474738545306508	29.845562823660554	20.809806178636915
22-23	21.558735646353377	27.3969646901673	29.67389784417013	21.370401819309183
24-25	21.320137567023075	27.463946252824094	29.869579620486775	21.346336559666053
26-27	21.339645759309267	27.644893922744007	29.701916949856678	21.31354336809005
28-29	21.208719715194192	27.771461402842224	29.738790227112393	21.281028654851188
30-31	21.60686452255695	27.53875846750433	29.641205565371337	21.213171444567376
32-33	21.371875902338864	27.679637418750552	29.607682970374317	21.340803708536267
34-35	21.466515284833854	27.737603198215037	29.615268899979096	21.180612616972013
36-37	21.43308725785197	27.640719175888595	29.597165799055237	21.329027767204202
38-39	21.24936508597749	27.787718563139364	29.70154417441749	21.261372176465652
40-41	21.575353762690735	27.618582595972363	29.520201846955658	21.28586179438124
42-43	21.43838165927417	27.703504498385605	29.356233517724874	21.501880324615353
44-45	21.547119264217756	27.83701594912279	29.19456851307884	21.421296273580616
46-47	21.605310915745214	27.81971946302727	28.92057218279829	21.654397438429232
48-49	21.542042174961683	27.917587827598116	28.9369469733519	21.6034230240883
50-51	21.533771904272424	28.06577284637644	28.75201641366707	21.64843883568406
52-53	21.663311530110494	28.01045665371144	28.692477558558473	21.633754257619593
54-55	21.64545720332304	27.972084887257964	28.685051581067434	21.69740632835156
56-57	21.639984743250686	28.04606236448366	28.65335226932665	21.660600622939004
58-59	21.608797601960408	28.137049206525393	28.677764385787146	21.576388805727053
60-61	21.71422363858064	28.10022122584404	28.663720538827263	21.521834596748054
62-63	21.631903016961154	28.088438471345288	28.673790921721583	21.605867589971975
64-65	21.6019585113719	28.062310146631347	28.659128096143117	21.67660324585363
66-67	21.7045328971857	28.132218121452212	28.462574403772784	21.7006745775893
68-69	21.63354220747296	28.073660836012383	28.57940841525458	21.713388541260077
70-71	21.65739787647371	27.81747758470361	28.67219895295985	21.852925585862838
72-73	21.73445077634657	27.862533987694672	28.649136062986617	21.75387917297214
74-75	21.64256333642219	27.999287245573644	28.55163427673255	21.806515141271618
76-77	21.660300802738597	27.9930254491126	28.498378694737347	21.848295053411455
78-79	21.67497448939492	28.02943330390529	28.474802927118404	21.82078927958139
80-81	21.64918862103322	28.014709155973964	28.469638860798103	21.866463362194715
82-83	21.746306238675185	28.00042003757996	28.550306202927306	21.702967520817555
84-85	21.87831305213777	28.007084909930093	28.412859176927828	21.701742861004313
86-87	21.659963495204654	28.038232981197442	28.524709329215135	21.77709419438277
88-89	21.76827067127886	28.068047096046268	28.43196492165437	21.731717311020507
90-91	21.81334144830934	28.045539568412224	28.489046409727557	21.652072573550882
92-93	21.843993383923404	28.0187553432457	28.432495159864036	21.704756112966862
94-95	21.679734264904972	28.164745016961955	28.407977851606287	21.747542866526793
96-97	21.69252597438975	28.163902802531698	28.458621106098047	21.684950116980506
98-99	21.94016256700734	28.748508983567866	28.486602502401382	20.824725947023413
100-101	22.527399022382298	31.24866747357825	25.908637119429244	20.315296384610203
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2671.0
1	2642.0
2	3481.0
3	4642.5
4	6069.0
5	8904.0
6	11345.5
7	13929.0
8	16885.5
9	18091.5
10	17180.5
11	15852.0
12	16764.5
13	20952.0
14	29919.5
15	40837.0
16	49026.5
17	53756.5
18	54115.5
19	50758.5
20	46275.5
21	42270.5
22	41892.0
23	47680.0
24	59183.5
25	75906.5
26	97557.0
27	121267.5
28	145636.0
29	170292.5
30	193049.0
31	218644.0
32	246928.0
33	274406.0
34	301861.0
35	327994.0
36	355060.5
37	380186.0
38	398529.5
39	411751.5
40	424061.0
41	437103.5
42	444624.0
43	449351.0
44	457137.5
45	460363.0
46	457135.5
47	453830.5
48	447910.5
49	435804.5
50	421307.5
51	404075.0
52	380388.0
53	354085.0
54	325951.0
55	296166.0
56	267897.0
57	237804.0
58	208645.0
59	180660.0
60	150421.5
61	120761.0
62	93326.0
63	70715.0
64	53323.5
65	39074.0
66	28173.0
67	20771.5
68	15273.5
69	10947.5
70	7886.5
71	5665.0
72	4061.5
73	2815.0
74	1788.0
75	1081.0
76	698.0
77	448.0
78	288.5
79	186.5
80	126.5
81	85.5
82	60.5
83	38.5
84	26.5
85	19.0
86	15.5
87	16.0
88	16.5
89	13.5
90	8.5
91	3.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06202672769687024
6	0.0
7	0.0034587052667986373
8	0.0
9	0.0
10-11	0.0
12-13	9.198684220209142E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.441622451446846E-5
28-29	2.7618149985334765E-5
30-31	3.225254145419153E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.4291027825965007E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	9.247322088655258E-4
54-55	1.535634979367258E-4
56-57	0.003144696312434264
58-59	0.007687755568817941
60-61	0.003159472417805792
62-63	0.005846640573477758
64-65	0.006607525451333129
66-67	0.004948582257146508
68-69	0.003682581390025181
70-71	0.005117102079367205
72-73	0.0030557592876550473
74-75	0.002834371688639053
76-77	0.0024200025035631284
78-79	6.572423589414125E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	7.399999817846159E-5
88-89	0.0
90-91	2.4991149936380087E-4
92-93	0.0
94-95	1.1882681336549738E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	148.0
20-21	618.0
22-23	1331.0
24-25	1595.0
26-27	3473.0
28-29	7451.0
30-31	19527.0
32-33	25327.0
34-35	34665.0
36-37	54280.0
38-39	66192.0
40-41	65150.0
42-43	73843.0
44-45	88737.0
46-47	88527.0
48-49	82440.0
50-51	75172.0
52-53	70636.0
54-55	75538.0
56-57	78722.0
58-59	78791.0
60-61	76968.0
62-63	74374.0
64-65	78095.0
66-67	83151.0
68-69	85962.0
70-71	85052.0
72-73	78995.0
74-75	80704.0
76-77	84784.0
78-79	87602.0
80-81	90103.0
82-83	84838.0
84-85	82896.0
86-87	87458.0
88-89	91487.0
90-91	94113.0
92-93	92446.0
94-95	100483.0
96-97	160255.0
98-99	896604.0
100-101	7282587.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.45061598539401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.64975414136637	73.97876590050757
2	7.750779617083873	12.936146668253713
3	1.9625588401197787	4.913302323467278
4	0.755336048142093	2.5213303397372355
5	0.34837586341919236	1.453609019838755
6	0.1825302148736475	0.9139355320291328
7	0.10783028119261488	0.629895237118137
8	0.0663623382628962	0.4430382405015816
9	0.04173319866935655	0.31343950223987666
>10	0.1319376422310667	1.6214766781741454
>50	0.00210222806441705	0.11347992165298838
>100	5.896570560803387E-4	0.08455415873734305
>500	7.328896872864435E-5	0.038545619553427876
>1k	3.664054993635107E-5	0.038480858188795146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.839736844041828E-5	0.0	1.839736844041828E-5	0.0
3	0.0	1.839736844041828E-5	0.0	1.839736844041828E-5	0.0
4	0.0	5.5192105321254847E-5	0.0	2.7596052660627423E-5	0.0
5	0.0	5.5192105321254847E-5	0.0	3.679473688083656E-5	0.0
6	0.0	7.358947376167312E-5	0.0	3.679473688083656E-5	0.0
7	0.0	7.358947376167312E-5	0.0	3.679473688083656E-5	0.0
8	9.19868422020914E-6	9.198684220209142E-5	0.0	3.679473688083656E-5	0.0
9	9.19868422020914E-6	1.2878157908292797E-4	0.0	6.439078954146399E-5	0.0
10-11	9.19868422020914E-6	1.2878157908292797E-4	0.0	8.278815798188228E-5	0.0
12-13	1.839736844041828E-5	1.2878157908292797E-4	0.0	1.0578486853240512E-4	0.0
14-15	3.679473688083657E-5	2.3916578972543766E-4	0.0	1.4717894752334628E-4	0.0
16-17	5.059276321115028E-5	2.3916578972543766E-4	0.0	1.6557631596376455E-4	0.0
18-19	5.9791447431359416E-5	2.667618423860651E-4	0.0	1.747750001839737E-4	0.0
20-21	6.439078954146399E-5	2.943578950466925E-4	0.0	1.9777171073449655E-4	0.0
22-23	6.439078954146399E-5	3.633480266982611E-4	0.0	2.3916578972543766E-4	0.0
24-25	7.358947376167312E-5	4.4153684257003877E-4	0.0	2.9435789504669255E-4	0.0
26-27	7.358947376167312E-5	4.691328952306662E-4	0.0	4.369375004599342E-4	0.0
28-29	7.358947376167312E-5	4.7833157945087533E-4	0.0	7.910868429379862E-4	0.0
30-31	9.198684220209142E-5	5.151263163317119E-4	0.0	0.0017063559228487958	0.0
32-33	9.198684220209142E-5	5.243250005519211E-4	0.0	0.003858848030377735	0.0
34-35	1.0118552642230055E-4	6.163118427540124E-4	0.0	0.006968003296808425	0.0
36-37	1.1038421064250969E-4	7.17497369176313E-4	0.0	0.01168232895966561	0.0
38-39	1.1038421064250969E-4	7.634907902773588E-4	0.0	0.01862733554592351	0.0
40-41	1.1038421064250969E-4	8.46278948259241E-4	0.0	0.026027677001081766	0.0
42-43	1.1038421064250969E-4	8.876730272501821E-4	0.0	0.0331152631927529	0.0
44-45	1.1958289486271883E-4	9.750605273421689E-4	0.0	0.04138947964883103	0.0
46-47	1.2878157908292797E-4	0.0010394513168836329	0.0	0.05028460728977327	0.0
48-49	1.379802633031371E-4	0.0010486500011038422	0.0	0.05905095335163259	0.0
50-51	1.379802633031371E-4	0.0010992427643149922	0.0	0.0680288691505567	0.0
52-53	1.6557631596376455E-4	0.0011038421064250969	0.0	0.07695619218626967	0.0
54-55	1.6557631596376455E-4	0.0011176401327554107	0.0	0.08580532640611087	0.0
56-57	1.6557631596376455E-4	0.0011682328959665609	0.0	0.09222600799181685	0.0
58-59	1.747750001839737E-4	0.0011728322380766655	0.0	0.09777281457660297	0.0
60-61	1.8397368440418283E-4	0.0011820309222968747	0.0	0.10320463760863646	0.0
62-63	1.8397368440418283E-4	0.0012234250012878158	0.0	0.10841569221938493	0.0
64-65	1.8397368440418283E-4	0.0014165973699122078	0.0	0.11348416722472018	0.0
66-67	1.8397368440418283E-4	0.0014211967120223122	0.0	0.118534244861615	0.0
68-69	1.8397368440418283E-4	0.001434994738352626	0.0	0.12413624355172237	0.0
70-71	1.8397368440418283E-4	0.0014487927646829398	0.0	0.12904374158320392	0.0
72-73	1.8397368440418283E-4	0.001457991448903149	0.0	0.13299457645578378	0.0
74-75	1.8397368440418283E-4	0.0014947861857839855	0.0	0.13634749685405	0.0
76-77	1.8397368440418283E-4	0.0015591769753254495	0.0	0.13921288698864515	0.0
78-79	1.8397368440418283E-4	0.0015913723700961815	0.0	0.1417747205439734	0.0
80-81	1.8397368440418283E-4	0.0015913723700961815	0.0	0.14301194357159153	0.0
82-83	1.9317236862439197E-4	0.0016005710543163906	0.0	0.14343968238783122	0.0
84-85	1.9317236862439197E-4	0.001637365791197227	0.0	0.14380762975663963	0.0
86-87	1.9317236862439197E-4	0.0016833592122982729	0.0	0.14399160344104378	0.0
88-89	1.9317236862439197E-4	0.0017707467123902598	0.0	0.14403299752003473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14955	0.0	48.948883	1
GTATCAA	27900	0.0	38.90678	1
TCAACGC	33110	0.0	32.47103	4
ATCAACG	33155	0.0	32.322563	3
CAACGCA	33820	0.0	31.738178	5
TATCAAC	34800	0.0	30.99359	2
AACGCAG	35145	0.0	30.621355	6
ACGCAGA	39935	0.0	26.87269	7
CGCAGAG	40420	0.0	26.538204	8
GTGGTAT	7500	0.0	24.34333	1
TGGTATC	7185	0.0	24.146067	2
GCAGAGT	47395	0.0	22.623524	9
GAGTACT	30255	0.0	20.435251	12-13
CAGAGTA	45935	0.0	19.398823	10-11
AGAGTAC	43380	0.0	18.931753	10-11
GTACATG	30485	0.0	18.832743	1
TACATGG	29610	0.0	18.644085	2
GTACTTT	33690	0.0	18.287989	14-15
ACATGGG	30295	0.0	17.59416	3
AGTACTT	32755	0.0	17.390581	12-13
>>END_MODULE