FastQCFastQC Report
Fri 27 May 2016
SRR2139683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8943899
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122000.051.2187041
GTATCAA222950.038.305271
TCAACGC255900.033.3385934
ATCAACG257750.033.0651133
CAACGCA264950.032.1333055
TATCAAC265250.031.7480812
AACGCAG274600.031.0861036
ACGCAGA320000.026.5794127
CGCAGAG326300.026.0525888
TGGTATC57450.025.2358592
GTGGTAT63250.024.8725261
GCAGAGT387800.022.0004969
GAGTACT222600.021.1228912-13
GTACATG293600.020.7876761
TACATGG291950.020.2259312
ACATGGG291200.019.7786393
CAGAGTA381500.019.36195210-11
GTACTTT251450.018.51590314-15
AGAGTAC350400.017.82472210-11
CATGGGG209850.017.2613624
Produced by FastQC (version 0.11.5)