##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8943899
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.553196542134476	30.0	18.0	32.0	18.0	33.0
2	27.989679221556504	30.0	25.0	32.0	18.0	33.0
3	27.609430629751074	29.0	25.0	31.0	18.0	33.0
4	30.80323100696911	32.0	30.0	33.0	27.0	33.0
5	31.27185045358853	33.0	32.0	33.0	28.0	33.0
6	35.13925693928341	37.0	35.0	38.0	29.0	38.0
7	35.66733255820532	38.0	36.0	38.0	31.0	38.0
8	36.119836326416475	38.0	37.0	38.0	33.0	38.0
9	36.59334827014482	38.0	37.0	38.0	34.0	38.0
10-11	36.690760707382765	38.0	38.0	38.0	34.0	38.0
12-13	36.786595476983805	38.0	38.0	38.0	34.5	38.0
14-15	36.79180142799019	38.0	38.0	38.0	34.5	38.0
16-17	36.810128949354194	38.0	38.0	38.0	35.0	38.0
18-19	36.823700547155106	38.0	38.0	38.0	35.0	38.0
20-21	36.86312529696423	38.0	38.0	38.0	35.0	38.0
22-23	36.85152403929658	38.0	38.0	38.0	35.0	38.0
24-25	36.859272067714066	38.0	38.0	38.0	35.0	38.0
26-27	36.819103090149014	38.0	38.0	38.0	35.0	38.0
28-29	36.73673743088666	38.0	38.0	38.0	34.5	38.0
30-31	36.77129786415598	38.0	38.0	38.0	35.0	38.0
32-33	36.77254447329909	38.0	38.0	38.0	35.0	38.0
34-35	36.76050458262273	38.0	38.0	38.0	35.0	38.0
36-37	36.732532538986035	38.0	38.0	38.0	35.0	38.0
38-39	36.72219095980523	38.0	38.0	38.0	34.0	38.0
40-41	36.72171905436251	38.0	38.0	38.0	34.5	38.0
42-43	36.68822976969146	38.0	38.0	38.0	34.0	38.0
44-45	36.632262757512976	38.0	38.0	38.0	34.0	38.0
46-47	36.5172100611954	38.0	38.0	38.0	34.0	38.0
48-49	36.46953822056604	38.0	38.0	38.0	34.0	38.0
50-51	36.48157430185766	38.0	38.0	38.0	34.0	38.0
52-53	36.49164967499952	38.0	38.0	38.0	34.0	38.0
54-55	36.417200096667656	38.0	38.0	38.0	34.0	38.0
56-57	36.43233029564469	38.0	38.0	38.0	34.0	38.0
58-59	36.374882096598256	38.0	38.0	38.0	34.0	38.0
60-61	36.391140346199634	38.0	38.0	38.0	34.0	38.0
62-63	36.40958776016794	38.0	38.0	38.0	34.0	38.0
64-65	36.400699054957286	38.0	38.0	38.0	34.0	38.0
66-67	36.38285200779176	38.0	38.0	38.0	34.0	38.0
68-69	36.36325353007847	38.0	38.0	38.0	34.0	38.0
70-71	36.362502465799025	38.0	38.0	38.0	34.0	38.0
72-73	36.356075070309444	38.0	38.0	38.0	34.0	38.0
74-75	36.32168898040541	38.0	38.0	38.0	33.5	38.0
76-77	36.31327471155525	38.0	37.5	38.0	33.0	38.0
78-79	36.297926588695674	38.0	37.0	38.0	33.0	38.0
80-81	36.301067985446906	38.0	37.0	38.0	33.0	38.0
82-83	36.272151485621734	38.0	37.0	38.0	33.0	38.0
84-85	36.24233726569148	38.0	37.0	38.0	33.0	38.0
86-87	36.24427513134469	38.0	37.0	38.0	33.0	38.0
88-89	36.2491448971524	38.0	37.0	38.0	33.0	38.0
90-91	36.19314744219441	38.0	37.0	38.0	33.0	38.0
92-93	36.21316649255621	38.0	37.0	38.0	33.0	38.0
94-95	36.16900411534408	38.0	37.0	38.0	33.0	38.0
96-97	36.194729873213554	38.0	37.0	38.0	33.0	38.0
98-99	36.23805332070704	38.0	37.0	38.0	33.0	38.0
100-101	35.706466136345355	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	8.0
19	27.0
20	119.0
21	460.0
22	1831.0
23	5595.0
24	12727.0
25	24391.0
26	40390.0
27	62068.0
28	87770.0
29	117952.0
30	151464.0
31	193691.0
32	248102.0
33	329520.0
34	466699.0
35	766474.0
36	1802180.0
37	4632431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.22475052546993	19.11962556822254	13.214605844721635	21.441018061585893
2	14.92015954115761	20.78754467151295	40.314576450382546	23.977719336946894
3	20.20733910344918	25.954295771899925	28.959238023595752	24.879127101055143
4	13.285883483254898	18.270588699626416	38.65866553278386	29.784862284334828
5	14.095221646504102	38.1075964850483	34.26299361962238	13.534188248825219
6	30.06859759932441	37.602224712063496	18.638873269924	13.69030441868809
7	26.790175524592495	32.91027967830778	22.597885618754823	17.7016591783449
8	24.129800660763276	37.236858332143505	20.738226136050955	17.89511487104226
9	25.345199001017342	17.8226297054562	21.392225024007985	35.43994626951847
10-11	24.30434422392292	27.09838852160562	28.230568122470974	20.366699132000484
12-13	25.095236713609843	24.47483611313344	28.743550701072916	21.686376472183802
14-15	22.28131153985527	25.867242016037974	26.438296094354374	25.413150349752385
16-17	21.717854819246057	29.10300082771507	27.18533046940713	21.993813883631734
18-19	21.72534595929583	28.0593396683035	29.041210103110508	21.17410426929016
20-21	22.467283690244884	27.15941341110838	29.101894425068703	21.271408473578035
22-23	22.254650605923075	27.09199414936125	28.945583491188987	21.707771753526693
24-25	22.01039819683776	27.242708760133617	29.03060683619994	21.716286206828688
26-27	21.915517738774135	27.381436417981025	28.999597435104096	21.703448408140748
28-29	21.81688321945415	27.340221049186002	29.068953527942593	21.77394220341725
30-31	22.14259322830523	27.229057848558497	28.974168334720446	21.654180588415826
32-33	21.91405230782252	27.38060959932424	28.96850942520025	21.736828667652986
34-35	22.073214210407002	27.380500136146747	28.934252044489465	21.61203360895679
36-37	22.06000683585117	27.278030509068202	28.872613857182515	21.789348797898118
38-39	21.818262715124774	27.407952115012797	29.009728546093466	21.764056623768965
40-41	22.065773495703073	27.264427771813804	28.901386769228726	21.7684119632544
42-43	22.00958485315974	27.294242670821646	28.772345334222056	21.923827141796558
44-45	21.981640285257527	27.448376387402245	28.638412071264934	21.931571256075294
46-47	22.15851910479574	27.42221548354123	28.342180161664448	22.077085249998586
48-49	22.048947802449884	27.514541869723836	28.35013896981668	22.086371358009597
50-51	22.047379462009875	27.701879866724475	28.250253973244	22.00048669802165
52-53	22.15357038462411	27.65828235229682	28.17027491855238	22.017872344526694
54-55	22.15316472642879	27.563620012787872	28.153554610628696	22.12966065015464
56-57	22.09922393523312	27.692479504754868	28.13697730453024	22.071319255481768
58-59	22.03351903675502	27.72897191295699	28.21499931908281	22.02250973120518
60-61	22.095621137240776	27.717932996288987	28.198871585383184	21.987574281087053
62-63	22.03343897745775	27.829711007208374	28.149793597308204	21.987056418025674
64-65	22.004203951334546	27.789812412834763	28.11263298755165	22.09335064827904
66-67	22.054907962244684	27.827210097173012	28.002658724811013	22.115223215771294
68-69	22.01148730417866	27.776233331559038	28.072085692764816	22.14019367149748
70-71	22.030800395480803	27.55953935599173	28.132667824758485	22.276992423768984
72-73	22.121973688717105	27.578903299680924	28.025426804658654	22.273696206943317
74-75	22.045115347432407	27.676344772243322	27.98783654848636	22.290703331837914
76-77	22.21065369367121	27.591761965356596	27.92490757186441	22.272676769107782
78-79	22.166178728139858	27.60907168940995	27.877274184476324	22.347475397973856
80-81	22.16708507818945	27.673328489454335	27.85319990609211	22.306386526264102
82-83	22.210628888056306	27.668818794967937	27.90074788216299	22.21980443481277
84-85	22.291901496688425	27.68885767152018	27.84626170363192	22.172979128159483
86-87	22.187100366084678	27.67694895467343	27.90644855480286	22.22950212443903
88-89	22.216740618043385	27.68442961171069	27.862962755885107	22.235867014360817
90-91	22.26845826949645	27.728265266831407	27.805451710611894	22.197824753060242
92-93	22.299325428805435	27.701966905573023	27.839706501973577	22.15900116364796
94-95	22.235834886662307	27.800601537769783	27.828102420443802	22.135461155124116
96-97	22.267825283497736	27.778020533192965	27.84668264005067	22.10747154325863
98-99	22.477061905644042	28.284124582558757	27.964876189432726	21.27393732236447
100-101	23.046020300866548	30.797832958590714	25.33450821077661	20.821638529766123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1533.0
1	1448.5
2	1976.5
3	2871.5
4	3568.0
5	5077.0
6	6794.5
7	8718.5
8	11543.0
9	13439.0
10	13009.0
11	11951.5
12	12409.0
13	14921.0
14	20223.5
15	26502.0
16	31951.5
17	36358.0
18	37809.5
19	35704.0
20	32422.5
21	29646.5
22	28701.0
23	31469.0
24	38572.0
25	49560.5
26	63813.0
27	81056.5
28	100595.5
29	121459.0
30	141830.0
31	162789.5
32	184503.5
33	205359.0
34	225249.5
35	243569.5
36	264227.0
37	283522.0
38	297159.5
39	308836.5
40	320494.5
41	331746.0
42	340000.0
43	347305.0
44	355117.5
45	362033.5
46	365399.0
47	365252.5
48	363065.0
49	356331.5
50	346798.0
51	337085.5
52	324393.5
53	307961.0
54	289605.5
55	269535.5
56	247548.0
57	223992.5
58	200223.0
59	173546.0
60	144331.5
61	117148.5
62	92313.0
63	70464.0
64	52747.0
65	38820.5
66	28453.0
67	21434.0
68	16185.0
69	11932.0
70	8584.0
71	6039.5
72	4074.5
73	2611.5
74	1677.0
75	1027.5
76	609.5
77	333.0
78	182.5
79	115.5
80	86.5
81	68.0
82	53.5
83	38.0
84	25.0
85	16.5
86	10.5
87	7.0
88	5.0
89	4.0
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.04635562186022002
6	0.0
7	0.0021802571786644727
8	0.0
9	0.0
10-11	0.0
12-13	5.5904030222165965E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.473562614974054E-5
28-29	1.1186924901166315E-5
30-31	5.597339069784937E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.735940693126485E-6
46-47	0.0
48-49	0.0
50-51	0.0
52-53	5.935395046671892E-4
54-55	1.830716157263006E-4
56-57	0.0029744316431080994
58-59	0.007551908776285121
60-61	0.0031722699387830003
62-63	0.0056531405856254605
64-65	0.006468041607214026
66-67	0.004871803545349321
68-69	0.0036987123973244544
70-71	0.0052034240023741555
72-73	0.0032931615712257214
74-75	0.002887551600768253
76-77	0.0024704197478732904
78-79	1.0271028036183292E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	5.31433317507692E-5
88-89	6.703959002877272E-6
90-91	1.6923853962304077E-4
92-93	0.0
94-95	1.312524704823556E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	87.0
20-21	354.0
22-23	778.0
24-25	919.0
26-27	1875.0
28-29	4259.0
30-31	11730.0
32-33	15203.0
34-35	21160.0
36-37	33490.0
38-39	38319.0
40-41	39570.0
42-43	45718.0
44-45	56136.0
46-47	56748.0
48-49	52116.0
50-51	47097.0
52-53	40946.0
54-55	44171.0
56-57	44930.0
58-59	52537.0
60-61	53366.0
62-63	51507.0
64-65	56831.0
66-67	61286.0
68-69	62281.0
70-71	65004.0
72-73	57510.0
74-75	59522.0
76-77	63185.0
78-79	66087.0
80-81	66809.0
82-83	64373.0
84-85	64538.0
86-87	67498.0
88-89	71675.0
90-91	74046.0
92-93	72711.0
94-95	81052.0
96-97	134377.0
98-99	780356.0
100-101	6261742.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.96498075029348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89793076234463	76.38175956713826
2	6.979559342511007	11.860362503639573
3	1.7158535364465453	4.373623880835111
4	0.6368903235675355	2.164534963278553
5	0.2892746940393836	1.228910940530163
6	0.15010999494725796	0.7652455698672255
7	0.09298773720609865	0.5530490911210664
8	0.058278960362775564	0.3961336596296274
9	0.04333646303555797	0.3313873572841789
>10	0.13280131213230878	1.715202881299225
>50	0.0023431215143195883	0.1295665810253241
>100	5.899158151921024E-4	0.07649779156744593
>500	4.383607742238775E-5	0.023725212784299215
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.354241813329958E-5	0.0	0.0	0.0
4	0.0	7.826564231103236E-5	0.0	4.472322417773278E-5	0.0
5	0.0	8.944644835546556E-5	0.0	4.472322417773278E-5	0.0
6	6.708483626659917E-5	8.944644835546556E-5	0.0	4.472322417773278E-5	1.1180806044433195E-5
7	6.708483626659917E-5	8.944644835546556E-5	0.0	4.472322417773278E-5	1.1180806044433195E-5
8	6.708483626659917E-5	8.944644835546556E-5	0.0	4.472322417773278E-5	1.1180806044433195E-5
9	6.708483626659917E-5	1.4535047857763153E-4	0.0	8.944644835546556E-5	1.1180806044433195E-5
10-11	6.708483626659917E-5	1.4535047857763153E-4	0.0	1.3976007555541493E-4	1.1180806044433195E-5
12-13	6.708483626659917E-5	1.4535047857763153E-4	0.0	1.6771209066649792E-4	1.1180806044433195E-5
14-15	6.708483626659917E-5	2.2361612088866387E-4	0.0	2.4038732995531367E-4	1.677120906664979E-5
16-17	8.385604533324896E-5	2.4597773297753027E-4	0.0	2.8511055413304645E-4	2.236161208886639E-5
18-19	1.0062725439989874E-4	2.6833934506639666E-4	0.0	3.2424337528856264E-4	2.236161208886639E-5
20-21	1.0062725439989874E-4	2.9070095715526305E-4	0.0	3.6896659946629543E-4	2.236161208886639E-5
22-23	1.0062725439989874E-4	2.9629136017747965E-4	0.0	4.0809942062181156E-4	4.472322417773277E-5
24-25	1.0062725439989874E-4	3.7455700248851197E-4	0.0	4.3046103271067795E-4	5.590403022216597E-5
26-27	1.0062725439989874E-4	4.0250901759959496E-4	0.0	4.8077465991062734E-4	5.590403022216597E-5
28-29	1.0062725439989874E-4	4.0809942062181156E-4	0.0	7.099811838215079E-4	5.590403022216597E-5
30-31	1.1180806044433194E-4	4.5282264479954435E-4	0.0	0.00161003607039838	5.590403022216597E-5
32-33	1.2298886648876513E-4	4.5841304782176095E-4	0.0	0.003790293249062853	6.708483626659917E-5
34-35	1.3976007555541493E-4	5.087266750217103E-4	0.0	0.006496048311815685	6.708483626659917E-5
36-37	1.4535047857763153E-4	5.646307052438763E-4	0.0	0.010442872845500602	6.708483626659917E-5
38-39	1.4535047857763153E-4	6.149443324438257E-4	0.0	0.01819117143429281	7.267523928881576E-5
40-41	1.5653128462206472E-4	7.435236019548075E-4	0.0	0.0465512859659976	7.826564231103236E-5
42-43	1.6212168764428132E-4	8.050180351991899E-4	0.0	0.0545008390635896	8.385604533324896E-5
44-45	1.6771209066649792E-4	8.721028714657891E-4	0.0	0.06554188503246738	8.944644835546556E-5
46-47	1.6771209066649792E-4	9.168260956435219E-4	0.0	0.0785507528651654	8.944644835546556E-5
48-49	1.6771209066649792E-4	9.224164986657385E-4	0.0	0.09119624450141935	8.944644835546556E-5
50-51	1.6771209066649792E-4	9.950917379545543E-4	0.0	0.10332182865660715	8.944644835546556E-5
52-53	1.6771209066649792E-4	0.0010062725439989875	0.0	0.12625366185373962	8.944644835546556E-5
54-55	1.7889289671093112E-4	0.0010230437530656371	0.0	0.18511501527465818	8.944644835546556E-5
56-57	1.8448329973314771E-4	0.00106776697724337	0.0	0.25062335788899226	8.944644835546556E-5
58-59	1.900737027553643E-4	0.0010845381863100198	0.0	0.32251035035167547	8.944644835546556E-5
60-61	2.0684491182201408E-4	0.0011180806044433194	0.0	0.3408301010554793	8.944644835546556E-5
62-63	2.3479692693309707E-4	0.0011348518135099691	0.0	0.3527041170746673	8.944644835546556E-5
64-65	2.4038732995531367E-4	0.001207527052798785	0.0	0.36133569934096976	8.944644835546556E-5
66-67	2.4597773297753027E-4	0.001235479067909868	0.0	0.36674720946647543	8.944644835546556E-5
68-69	2.4597773297753027E-4	0.0012746118890653842	0.0	0.37196864588922574	8.944644835546556E-5
70-71	2.4597773297753027E-4	0.0013025639041764673	0.0	0.37716213029686496	8.944644835546556E-5
72-73	2.6274894204418006E-4	0.001319335113243117	0.0	0.3807958922613057	1.0062725439989874E-4
74-75	2.7392974808861326E-4	0.0013472871283542	0.0	0.3837029018328584	1.0062725439989874E-4
76-77	2.9070095715526305E-4	0.001431143173687449	0.0	0.3863415720593446	1.0062725439989874E-4
78-79	2.9070095715526305E-4	0.0014423239797318821	0.0	0.3886504085075201	1.0062725439989874E-4
80-81	2.9070095715526305E-4	0.0014423239797318821	0.0	0.3897125850817412	1.0621765742211534E-4
82-83	2.9070095715526305E-4	0.0014646855918207485	0.0	0.39013745571142966	1.2298886648876513E-4
84-85	2.9070095715526305E-4	0.001487047203909615	0.0	0.3904952415048515	1.2298886648876513E-4
86-87	2.9629136017747965E-4	0.0015038184129762648	0.0	0.3906573631924958	1.2857926951098173E-4
88-89	3.0188176319969625E-4	0.0016044456673761634	0.0	0.3907244480287624	1.3416967253319833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12200	0.0	51.218704	1
GTATCAA	22295	0.0	38.30527	1
TCAACGC	25590	0.0	33.338593	4
ATCAACG	25775	0.0	33.065113	3
CAACGCA	26495	0.0	32.133305	5
TATCAAC	26525	0.0	31.748081	2
AACGCAG	27460	0.0	31.086103	6
ACGCAGA	32000	0.0	26.579412	7
CGCAGAG	32630	0.0	26.052588	8
TGGTATC	5745	0.0	25.235859	2
GTGGTAT	6325	0.0	24.872526	1
GCAGAGT	38780	0.0	22.000496	9
GAGTACT	22260	0.0	21.12289	12-13
GTACATG	29360	0.0	20.787676	1
TACATGG	29195	0.0	20.225931	2
ACATGGG	29120	0.0	19.778639	3
CAGAGTA	38150	0.0	19.361952	10-11
GTACTTT	25145	0.0	18.515903	14-15
AGAGTAC	35040	0.0	17.824722	10-11
CATGGGG	20985	0.0	17.261362	4
>>END_MODULE