FastQCFastQC Report
Fri 27 May 2016
SRR2139682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9252176
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130200.048.3629381
GTATCAA230550.037.113841
TCAACGC262650.032.4952134
ATCAACG262950.032.4250833
CTTATAC116200.031.937951
CAACGCA268300.031.8433065
AACGCAG277900.030.8392416
TATCAAC273950.030.8375762
TATACAC159150.026.7592983
ACGCAGA322950.026.5104127
CGCAGAG326650.026.196128
TTATACA156550.025.7047392
GCAGAGT385800.022.1460119
GTGGTAT63650.021.7794821
TGGTATC60600.021.0828822
TACACAT211450.020.5105885
GAGTACT226800.020.29136712-13
ACACATC200000.020.2636096
CAGAGTA377850.019.56561510-11
GTACATG287850.018.8409861
Produced by FastQC (version 0.11.5)