##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9252176
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.315805168427406	30.0	18.0	32.0	18.0	33.0
2	27.732141282223772	29.0	25.0	32.0	18.0	33.0
3	27.35357963359106	29.0	25.0	31.0	18.0	33.0
4	30.702444376328337	32.0	30.0	33.0	27.0	33.0
5	31.212126423016596	33.0	32.0	33.0	28.0	33.0
6	35.09152852258755	37.0	35.0	38.0	29.0	38.0
7	35.63320293517979	38.0	36.0	38.0	31.0	38.0
8	36.09668374228938	38.0	37.0	38.0	33.0	38.0
9	36.58207496268986	38.0	37.0	38.0	34.0	38.0
10-11	36.6750119107116	38.0	38.0	38.0	34.0	38.0
12-13	36.7730790572942	38.0	38.0	38.0	34.0	38.0
14-15	36.77563029497061	38.0	38.0	38.0	34.0	38.0
16-17	36.792569066995696	38.0	38.0	38.0	35.0	38.0
18-19	36.8027356483491	38.0	38.0	38.0	35.0	38.0
20-21	36.844007480625024	38.0	38.0	38.0	35.0	38.0
22-23	36.83064914281914	38.0	38.0	38.0	35.0	38.0
24-25	36.83469515395858	38.0	38.0	38.0	35.0	38.0
26-27	36.79038309583191	38.0	38.0	38.0	35.0	38.0
28-29	36.700853411787094	38.0	38.0	38.0	34.0	38.0
30-31	36.741238050861114	38.0	38.0	38.0	35.0	38.0
32-33	36.73743617250328	38.0	38.0	38.0	34.5	38.0
34-35	36.7136756604351	38.0	38.0	38.0	34.0	38.0
36-37	36.68614866789439	38.0	38.0	38.0	34.0	38.0
38-39	36.65695002936954	38.0	38.0	38.0	34.0	38.0
40-41	36.68744720496097	38.0	38.0	38.0	34.0	38.0
42-43	36.660224717840265	38.0	38.0	38.0	34.0	38.0
44-45	36.599221110113106	38.0	38.0	38.0	34.0	38.0
46-47	36.48226409952308	38.0	38.0	38.0	34.0	38.0
48-49	36.43475150015722	38.0	38.0	38.0	34.0	38.0
50-51	36.44261790615475	38.0	38.0	38.0	34.0	38.0
52-53	36.446740787699135	38.0	38.0	38.0	34.0	38.0
54-55	36.36643254270608	38.0	38.0	38.0	34.0	38.0
56-57	36.37815789084375	38.0	38.0	38.0	34.0	38.0
58-59	36.316402891188105	38.0	38.0	38.0	33.5	38.0
60-61	36.32321301453338	38.0	38.0	38.0	33.0	38.0
62-63	36.338361736947945	38.0	38.0	38.0	33.5	38.0
64-65	36.3232320064274	38.0	38.0	38.0	33.5	38.0
66-67	36.29896397258527	38.0	38.0	38.0	33.0	38.0
68-69	36.27248795453684	38.0	37.5	38.0	33.0	38.0
70-71	36.268926679963656	38.0	37.0	38.0	33.0	38.0
72-73	36.259143742885115	38.0	37.5	38.0	33.0	38.0
74-75	36.22438429770398	38.0	37.0	38.0	33.0	38.0
76-77	36.2165273080162	38.0	37.0	38.0	33.0	38.0
78-79	36.20537267442823	38.0	37.0	38.0	33.0	38.0
80-81	36.21306835957701	38.0	37.0	38.0	33.0	38.0
82-83	36.182983683487514	38.0	37.0	38.0	33.0	38.0
84-85	36.15506013487482	38.0	37.0	38.0	33.0	38.0
86-87	36.156885347914155	38.0	37.0	38.0	33.0	38.0
88-89	36.164772230641994	38.0	37.0	38.0	33.0	38.0
90-91	36.108224374095066	38.0	37.0	38.0	33.0	38.0
92-93	36.135106966315035	38.0	37.0	38.0	33.0	38.0
94-95	36.09647489227442	38.0	37.0	38.0	33.0	38.0
96-97	36.12571536783953	38.0	37.0	38.0	33.0	38.0
98-99	36.17109464922636	38.0	37.0	38.0	33.0	38.0
100-101	35.63584252738684	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	32.0
19	120.0
20	283.0
21	724.0
22	2400.0
23	6575.0
24	14438.0
25	26828.0
26	44608.0
27	67290.0
28	95287.0
29	127563.0
30	163255.0
31	206196.0
32	264220.0
33	349107.0
34	498656.0
35	829125.0
36	1967849.0
37	4587617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.95737262239715	19.24314885492883	13.166286503845148	21.63319201882887
2	14.65128851850635	20.725329911579717	40.47241427313964	24.150967296774294
3	20.12248794229595	26.06181508004171	29.15490366806684	24.660793309595494
4	13.087958983919027	18.651644759027498	38.4797262827685	29.78066997428497
5	13.980445773559557	38.139911184391224	34.372319668717	13.50732337333222
6	29.73722073596525	37.68643181884996	18.753458645836396	13.82288879934839
7	26.404879297820028	32.992664170410094	22.362493591917065	18.239962939852813
8	23.90488464551474	37.24804845908681	21.015585955130987	17.831480940267458
9	24.970579893854158	17.8410354493905	21.158103780126964	36.030280876628375
10-11	24.184840409434493	27.00973803351774	28.34197598489264	20.463445572155134
12-13	25.03832071504044	24.369850941011066	28.573375603749863	22.01845274019863
14-15	22.222285870912962	25.845271425878625	26.196680651124666	25.73576205208375
16-17	21.818834834097405	29.11293516249583	26.911739465397112	22.156490538009656
18-19	21.724170616728433	27.937741348629768	28.77724656340303	21.560841471238763
20-21	22.422007484575026	27.030992292823523	28.85614580216314	21.690854420438306
22-23	22.07772849424519	27.25578182239153	28.690314006825062	21.976175676538222
24-25	21.8700731221109	27.34046428864856	29.009002970720886	21.780459618519657
26-27	21.824464138628834	27.38194788184441	28.953278463239478	21.840309516287277
28-29	21.4538921654379	27.142827088799322	29.09524684877216	22.30803389699062
30-31	21.795643366981	26.98184598196124	29.15130589582381	22.071204755233957
32-33	21.62909222594374	27.264428397552543	29.15561575942637	21.950863617077342
34-35	21.75092635743254	27.530772393898605	29.19962706951484	21.51867417915402
36-37	21.91849616298147	27.289317703736664	28.74895902886072	22.043227104421142
38-39	21.783590313145197	27.285576620898144	28.987129586342398	21.943703479614264
40-41	22.05459456046011	27.043939857619343	29.020949722520673	21.880515859399875
42-43	22.064746123261124	27.042543590718637	28.988656678757014	21.904053607263226
44-45	21.91827720300902	27.326994348322035	28.790518327402125	21.96421012126682
46-47	22.25475874881938	27.11820359296287	28.26355307963334	22.363484578584412
48-49	22.07743870358463	27.24040138339832	28.384719277159554	22.297440635857498
50-51	21.96819548313948	27.48237483579457	28.416270874810113	22.13315880625584
52-53	22.03405576337269	27.56956060590624	28.50310016198858	21.893283468732484
54-55	22.03135018991535	27.571025584577846	28.23027776082513	22.167346464681675
56-57	22.17084107265379	27.904571774954217	27.902776924705915	22.02181022768608
58-59	21.844724435499437	28.256153295200377	28.204368181132928	21.694754088167265
60-61	22.096380395772496	28.407531735283026	27.817352978017805	21.678734890926673
62-63	21.82931933129811	28.653601447743455	27.768412527757114	21.748666693201322
64-65	21.80773560894548	28.579184148776832	27.732206648074726	21.880873594202956
66-67	21.879493388532445	28.456150346863378	27.691662853038423	21.97269341156575
68-69	21.93047712450753	28.186945728105865	27.857675629341365	22.024901518045233
70-71	21.97799722674294	27.76836713522694	28.04281550501798	22.210820133012135
72-73	22.091646559694514	27.617611652187417	28.024903178220857	22.26583860989721
74-75	22.01205624080354	27.695658665597755	28.005247711522408	22.287037382076303
76-77	22.15155684552296	27.617580130159364	27.932573302674264	22.298289721643414
78-79	22.10686357741813	27.662328855200354	27.863668904347005	22.367138663034513
80-81	22.056184010873917	27.727738781271754	27.911350034492454	22.304727173361876
82-83	22.153070282950228	27.66546772777479	27.971599178744768	22.20986281053022
84-85	22.293877574605563	27.682634692006015	27.825058213726685	22.198429519661737
86-87	22.120630500325746	27.700533873776063	27.949807452373154	22.22902817352504
88-89	22.197321768695385	27.653123972524803	27.940252104222402	22.209302154557406
90-91	22.20458476399051	27.68999104812544	27.900096809865154	22.205327378018893
92-93	22.181216145039905	27.630918574618725	27.961616020551027	22.226249259790336
94-95	22.063195790391706	27.743914347408417	27.977189803335072	22.2157000588648
96-97	22.11710447486927	27.74743242634682	27.961390507237923	22.174072591545993
98-99	22.36946994699254	28.344309189324356	27.99962060543612	21.28660025824698
100-101	22.988291207078145	30.827280164136507	25.389457311979523	20.794971316805825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2013.0
1	1924.5
2	2322.0
3	3021.0
4	3831.5
5	5640.5
6	7452.5
7	9168.0
8	11473.5
9	12879.0
10	12349.0
11	11403.5
12	11883.0
13	14599.5
14	20580.5
15	27738.0
16	33769.0
17	38635.5
18	40208.0
19	38016.5
20	34585.0
21	31201.5
22	29831.5
23	32341.0
24	38895.5
25	49396.5
26	63652.5
27	80195.0
28	98717.5
29	120168.5
30	141765.0
31	163583.0
32	186557.5
33	210005.0
34	232518.0
35	252193.0
36	275037.0
37	299332.5
38	317404.5
39	330560.5
40	343876.0
41	357795.5
42	367021.5
43	378143.5
44	408732.0
45	430381.0
46	426244.0
47	417633.5
48	399457.0
49	381113.0
50	370349.0
51	356497.5
52	337844.0
53	318080.5
54	298008.0
55	275599.5
56	252359.0
57	227378.0
58	201342.5
59	174137.5
60	144907.0
61	116883.0
62	91555.0
63	70486.0
64	53309.0
65	39159.5
66	28367.5
67	21349.5
68	16342.5
69	11914.5
70	8501.5
71	6017.5
72	4137.5
73	2734.0
74	1742.5
75	1040.5
76	659.0
77	397.5
78	265.5
79	194.0
80	123.0
81	71.0
82	50.0
83	42.5
84	32.0
85	21.0
86	15.0
87	13.0
88	10.0
89	6.0
90	3.0
91	1.5
92	1.0
93	1.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.04595675655110754
6	0.0
7	0.002042762697121196
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.109193863416199E-5
28-29	2.163273617066498E-5
30-31	2.1654263375283598E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.1182693395316766E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	6.10122753820131E-4
54-55	1.3896649459912508E-4
56-57	0.003088445623777442
58-59	0.007695609549172189
60-61	0.0032629337640405903
62-63	0.005838871920412124
64-65	0.00670686784468167
66-67	0.00485494238286512
68-69	0.0038569067987043
70-71	0.005259370171240762
72-73	0.003217345226640418
74-75	0.003008745125423758
76-77	0.00255683128536223
78-79	6.397633387457312E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	5.2993212298161275E-5
88-89	0.0
90-91	1.6202461639996965E-4
92-93	0.0
94-95	1.1022068868504031E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	129.0
20-21	464.0
22-23	1063.0
24-25	1271.0
26-27	2724.0
28-29	6230.0
30-31	17634.0
32-33	22302.0
34-35	29879.0
36-37	46615.0
38-39	52865.0
40-41	53015.0
42-43	58754.0
44-45	69672.0
46-47	70218.0
48-49	63504.0
50-51	56518.0
52-53	50757.0
54-55	54290.0
56-57	55018.0
58-59	61605.0
60-61	69147.0
62-63	64167.0
64-65	77165.0
66-67	81759.0
68-69	76411.0
70-71	90377.0
72-73	60146.0
74-75	61595.0
76-77	64671.0
78-79	67560.0
80-81	68380.0
82-83	65418.0
84-85	65774.0
86-87	68455.0
88-89	72012.0
90-91	74176.0
92-93	72928.0
94-95	80323.0
96-97	134074.0
98-99	782161.0
100-101	6280950.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.07848480967586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.92639041262791	76.50801040709744
2	6.942985305727249	11.81397339734237
3	1.7259172522973334	4.405152741970085
4	0.6462623635520899	2.1993209072212676
5	0.28825550788044485	1.2262170924255915
6	0.16142900387329068	0.824048103232492
7	0.08931147044317858	0.5318939207000849
8	0.05633467091967541	0.38342947552781537
9	0.03840887253090997	0.294099181036002
>10	0.12167207957229877	1.5872186045708854
>50	0.0024210641095077975	0.13258084398774975
>100	5.704022891102698E-4	0.07204418053971583
>500	4.159417699727161E-5	0.02201114434854755
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0808268238736487E-5	1.0808268238736487E-5
3	0.0	0.0	0.0	1.0808268238736487E-5	1.0808268238736487E-5
4	0.0	2.1616536477472974E-5	0.0	1.0808268238736487E-5	2.1616536477472974E-5
5	0.0	2.1616536477472974E-5	0.0	2.1616536477472974E-5	2.1616536477472974E-5
6	9.727441414862839E-5	2.1616536477472974E-5	0.0	2.1616536477472974E-5	2.1616536477472974E-5
7	9.727441414862839E-5	2.1616536477472974E-5	0.0	3.242480471620946E-5	3.242480471620946E-5
8	9.727441414862839E-5	2.1616536477472974E-5	0.0	4.323307295494595E-5	5.404134119368244E-5
9	9.727441414862839E-5	1.5131575534231082E-4	0.0	5.404134119368244E-5	5.404134119368244E-5
10-11	9.727441414862839E-5	1.5131575534231082E-4	0.0	8.64661459098919E-5	5.404134119368244E-5
12-13	9.727441414862839E-5	1.621240235810473E-4	0.0	9.727441414862839E-5	5.404134119368244E-5
14-15	9.727441414862839E-5	2.485901694909392E-4	0.0	9.727441414862839E-5	1.0808268238736488E-4
16-17	1.0808268238736488E-4	2.485901694909392E-4	0.0	1.1889095062610136E-4	1.0808268238736488E-4
18-19	1.1889095062610136E-4	2.539943036103075E-4	0.0	1.3510335298420607E-4	1.0808268238736488E-4
20-21	1.1889095062610136E-4	2.6480257184904394E-4	0.0	1.621240235810473E-4	1.1348681650673312E-4
22-23	1.1889095062610136E-4	2.9722737656525343E-4	0.0	1.729322918197838E-4	1.242950847454696E-4
24-25	1.1889095062610136E-4	3.242480471620946E-4	0.0	2.0535709653599326E-4	1.621240235810473E-4
26-27	1.1889095062610136E-4	3.242480471620946E-4	0.0	3.458645836395676E-4	1.621240235810473E-4
28-29	1.1889095062610136E-4	3.242480471620946E-4	0.0	7.457705084728177E-4	1.621240235810473E-4
30-31	1.2969921886483784E-4	3.9990592483325006E-4	0.0	0.002204886720702243	1.621240235810473E-4
32-33	1.4050748710357433E-4	3.9990592483325006E-4	0.0	0.00532307210757772	1.8374056005852028E-4
34-35	1.4050748710357433E-4	4.539472660269325E-4	0.0	0.009208644539403487	2.0535709653599326E-4
36-37	1.5131575534231082E-4	4.971803389818784E-4	0.0	0.014958643242411298	2.0535709653599326E-4
38-39	1.5131575534231082E-4	5.07988607220615E-4	0.0	0.02539402622691138	2.1616536477472975E-4
40-41	1.5131575534231082E-4	5.07988607220615E-4	0.0	0.053630627000610456	2.1616536477472975E-4
42-43	1.621240235810473E-4	5.350092778174561E-4	0.0	0.06382282394973895	2.2156949889409798E-4
44-45	1.7833642593915203E-4	5.998588872498751E-4	0.0	0.0769710822621619	2.323777671328345E-4
46-47	1.8374056005852028E-4	6.484960943241892E-4	0.0	0.09143794929971069	2.3778190125220273E-4
48-49	1.8374056005852028E-4	6.484960943241892E-4	0.0	0.10415928101670352	2.593984377296757E-4
50-51	2.1076123065536152E-4	7.241539719953446E-4	0.0	0.11668065977127975	2.593984377296757E-4
52-53	2.2156949889409798E-4	7.349622402340811E-4	0.0	0.1366165105376292	2.6480257184904394E-4
54-55	2.2697363301346624E-4	7.403663743534494E-4	0.0	0.17836885074386827	2.756108400877804E-4
56-57	2.2697363301346624E-4	7.457705084728177E-4	0.0	0.2250227405963743	2.864191083265169E-4
58-59	2.2697363301346624E-4	7.565787767115542E-4	0.0	0.2768483868011158	2.918232424458852E-4
60-61	2.3778190125220273E-4	7.781953131890272E-4	0.0	0.29594119264484375	2.918232424458852E-4
62-63	2.702067059684122E-4	8.21428386143973E-4	0.0	0.30636036322698573	2.918232424458852E-4
64-65	2.8101497420714866E-4	9.187028002926015E-4	0.0	0.31561764497346356	2.918232424458852E-4
66-67	3.1884391304272636E-4	9.295110685313379E-4	0.0	0.32080561372805705	2.9722737656525343E-4
68-69	3.242480471620946E-4	9.565317391281792E-4	0.0	0.3257990336543533	3.0263151068462164E-4
70-71	3.242480471620946E-4	9.727441414862838E-4	0.0	0.3303276980463839	3.3505631540083113E-4
72-73	3.458645836395676E-4	9.835524097250204E-4	0.0	0.33411599606406106	3.8909765659451355E-4
74-75	3.458645836395676E-4	0.0010051689462024934	0.0	0.3371152905003104	3.8909765659451355E-4
76-77	3.5126871775893585E-4	0.001037593750918703	0.0	0.33982276169411385	3.945017907138818E-4
78-79	3.566728518783041E-4	0.001064614421515544	0.0	0.34261129489970793	3.9990592483325006E-4
80-81	3.6748112011704057E-4	0.0010700185556349123	0.0	0.3437893961377302	4.107141930719865E-4
82-83	3.6748112011704057E-4	0.0010700185556349123	0.0	0.34426495994023465	4.4313899778819596E-4
84-85	3.8909765659451355E-4	0.001086230957993017	0.0	0.3446864824015453	4.4313899778819596E-4
86-87	3.8909765659451355E-4	0.0011024433603511218	0.0	0.3448107774862908	4.4313899778819596E-4
88-89	3.9990592483325006E-4	0.0011348681650673311	0.0	0.34487022296160386	4.485431319075642E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13020	0.0	48.362938	1
GTATCAA	23055	0.0	37.11384	1
TCAACGC	26265	0.0	32.495213	4
ATCAACG	26295	0.0	32.425083	3
CTTATAC	11620	0.0	31.93795	1
CAACGCA	26830	0.0	31.843306	5
AACGCAG	27790	0.0	30.839241	6
TATCAAC	27395	0.0	30.837576	2
TATACAC	15915	0.0	26.759298	3
ACGCAGA	32295	0.0	26.510412	7
CGCAGAG	32665	0.0	26.19612	8
TTATACA	15655	0.0	25.704739	2
GCAGAGT	38580	0.0	22.146011	9
GTGGTAT	6365	0.0	21.779482	1
TGGTATC	6060	0.0	21.082882	2
TACACAT	21145	0.0	20.510588	5
GAGTACT	22680	0.0	20.291367	12-13
ACACATC	20000	0.0	20.263609	6
CAGAGTA	37785	0.0	19.565615	10-11
GTACATG	28785	0.0	18.840986	1
>>END_MODULE