FastQCFastQC Report
Fri 27 May 2016
SRR2139681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9437271
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132950.048.2121161
CTTATAC97300.044.140041
GTATCAA234550.037.6544761
TATACAC144100.034.876953
TCAACGC271000.032.717564
TTATACA144400.032.677882
ATCAACG273700.032.3315963
CAACGCA275700.032.1284335
TATCAAC280850.031.2928852
AACGCAG285800.031.1168636
ACGCAGA332050.026.7045887
CGCAGAG338900.026.164278
TACACAT199350.025.1022975
GTGGTAT65700.024.8183161
ACACATC191000.024.5821696
ACATCTC195600.023.8930388
TGGTATC64350.023.3226172
GCAGAGT402250.022.1296639
CACATCT214750.021.6622147
ATACACA253200.021.3009574
Produced by FastQC (version 0.11.5)