##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9437271
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.112741490627958	28.0	18.0	32.0	18.0	33.0
2	27.585754504665598	29.0	25.0	32.0	18.0	33.0
3	27.256223647704935	29.0	25.0	31.0	18.0	33.0
4	30.657692250227846	32.0	30.0	33.0	27.0	33.0
5	31.185250163950997	33.0	32.0	33.0	28.0	33.0
6	35.05992781175829	37.0	34.0	38.0	29.0	38.0
7	35.61276750450421	38.0	36.0	38.0	31.0	38.0
8	36.08885195730842	38.0	37.0	38.0	33.0	38.0
9	36.578532607572676	38.0	37.0	38.0	34.0	38.0
10-11	36.675195032547016	38.0	38.0	38.0	34.0	38.0
12-13	36.77465111471314	38.0	38.0	38.0	34.0	38.0
14-15	36.77761918673311	38.0	38.0	38.0	34.0	38.0
16-17	36.795000376697885	38.0	38.0	38.0	35.0	38.0
18-19	36.808982013974166	38.0	38.0	38.0	35.0	38.0
20-21	36.847796012919574	38.0	38.0	38.0	35.0	38.0
22-23	36.835270319570284	38.0	38.0	38.0	35.0	38.0
24-25	36.8394396733805	38.0	38.0	38.0	35.0	38.0
26-27	36.79821409411552	38.0	38.0	38.0	35.0	38.0
28-29	36.71540841769149	38.0	38.0	38.0	34.0	38.0
30-31	36.74948466020642	38.0	38.0	38.0	35.0	38.0
32-33	36.73451291016733	38.0	38.0	38.0	34.5	38.0
34-35	36.70546821459783	38.0	38.0	38.0	34.0	38.0
36-37	36.67642686267159	38.0	38.0	38.0	34.0	38.0
38-39	36.63503985219243	38.0	38.0	38.0	34.0	38.0
40-41	36.685100678972645	38.0	38.0	38.0	34.0	38.0
42-43	36.66203435918756	38.0	38.0	38.0	34.0	38.0
44-45	36.602769228841844	38.0	38.0	38.0	34.0	38.0
46-47	36.48584848798427	38.0	38.0	38.0	34.0	38.0
48-49	36.43649434703873	38.0	38.0	38.0	34.0	38.0
50-51	36.44717313521529	38.0	38.0	38.0	34.0	38.0
52-53	36.45371204054072	38.0	38.0	38.0	34.0	38.0
54-55	36.37939402785459	38.0	38.0	38.0	34.0	38.0
56-57	36.395358132311245	38.0	38.0	38.0	34.0	38.0
58-59	36.33603346812493	38.0	38.0	38.0	33.5	38.0
60-61	36.350043317121504	38.0	38.0	38.0	33.0	38.0
62-63	36.36866729477185	38.0	38.0	38.0	34.0	38.0
64-65	36.35769697947386	38.0	38.0	38.0	34.0	38.0
66-67	36.33212464776807	38.0	38.0	38.0	33.0	38.0
68-69	36.30565356876596	38.0	38.0	38.0	33.0	38.0
70-71	36.30225151958622	38.0	38.0	38.0	33.0	38.0
72-73	36.292699497926975	38.0	38.0	38.0	33.0	38.0
74-75	36.25947837548437	38.0	37.5	38.0	33.0	38.0
76-77	36.255822353093855	38.0	37.0	38.0	33.0	38.0
78-79	36.24402876138775	38.0	37.0	38.0	33.0	38.0
80-81	36.25271903302004	38.0	37.0	38.0	33.0	38.0
82-83	36.22426028735426	38.0	37.0	38.0	33.0	38.0
84-85	36.19960712933653	38.0	37.0	38.0	33.0	38.0
86-87	36.202175091431585	38.0	37.0	38.0	33.0	38.0
88-89	36.20918354305546	38.0	37.0	38.0	33.0	38.0
90-91	36.15390795137645	38.0	37.0	38.0	33.0	38.0
92-93	36.176657415150274	38.0	37.0	38.0	33.0	38.0
94-95	36.138203475099445	38.0	37.0	38.0	33.0	38.0
96-97	36.1696668740301	38.0	37.0	38.0	33.0	38.0
98-99	36.21904290261321	38.0	37.0	38.0	33.0	38.0
100-101	35.68864104688976	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	47.0
19	138.0
20	354.0
21	874.0
22	2559.0
23	6370.0
24	14306.0
25	26802.0
26	44706.0
27	68186.0
28	95761.0
29	129359.0
30	165886.0
31	210994.0
32	270077.0
33	355582.0
34	506766.0
35	843898.0
36	2018757.0
37	4675845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.36549061693788	19.241749018333795	13.01561648489272	21.3771438798356
2	14.68378941327424	20.81635676245813	40.409669278332686	24.090184545934942
3	20.010933245426564	26.12892010836607	29.333670719003408	24.526475927203954
4	13.002381726666535	18.82298389015214	38.75644770612182	29.4181866770595
5	13.862288275068527	38.233517822914806	34.53887092545754	13.365322976559128
6	29.43233271567596	37.98123419365619	18.817431437541636	13.769001653126207
7	26.264345782716585	33.06615362737724	22.508793490877594	18.160707099028578
8	23.79252434310724	37.1456112683423	21.223275245566224	17.838589142984237
9	24.87283664949327	17.836406308561024	21.290127198848058	36.00062984309765
10-11	23.92549180796016	27.090797752867328	28.62195013791593	20.36176030125658
12-13	24.92874528332876	24.40204105636482	28.840263126942432	21.82895053336399
14-15	22.117161836297804	25.867329654939443	26.399882974643834	25.61562553411892
16-17	21.636668057958705	29.30310573893661	27.04903779916885	22.01118840393584
18-19	21.63109971092279	28.039202222761222	28.967134672724775	21.36256339359122
20-21	22.299065453241692	27.135288103482086	29.035440105681502	21.530206337594716
22-23	21.98162454642762	27.42721660525425	28.86726668400037	21.72389216431776
24-25	21.85258180121249	27.5744285590812	29.104124971098933	21.468864668607377
26-27	21.99522208941709	27.5766416958747	29.067437144897113	21.3606990698111
28-29	21.688363523234454	27.33885057768699	29.30377697268547	21.66900892639309
30-31	22.15715714573215	27.353330315547275	29.038346391973462	21.45116614674711
32-33	21.87425554460008	27.30698848313199	29.149859729839893	21.668896242428033
34-35	21.671869600282612	27.59367014286435	29.406748204366412	21.32771205248663
36-37	22.016495288850727	27.35539253650094	29.00615863250808	21.62195354214026
38-39	21.602972279074418	27.33148131450677	29.294904589035927	21.770641817382884
40-41	21.888117523690635	27.176334582920024	29.24548799051101	21.690059902878332
42-43	22.00418282698179	27.148598661344852	29.083010263364535	21.764208248308822
44-45	21.79913000362453	27.48533077298232	28.92756625913534	21.78797296425781
46-47	22.18037746698431	27.231230173205823	28.45520172815275	22.133190631657115
48-49	22.00037408146051	27.317926963191848	28.48206980869403	22.199629146653617
50-51	21.812967596481645	27.5054619151334	28.653766625574534	22.027803862810423
52-53	21.928197395134685	27.50598761012218	28.799096137113438	21.766718857629698
54-55	21.920608636778805	27.468592059244063	28.50540654104555	22.10539276293158
56-57	22.123002127096854	27.875217058319496	28.111783914349942	21.88999690023371
58-59	21.78344176147907	28.232319467079463	28.434471838510554	21.549766932930908
60-61	22.02842343346611	28.535846446819985	27.930014505013638	21.505715614700264
62-63	21.70139138940062	28.86127298423841	27.893941632319557	21.543393994041413
64-65	21.726879614141605	28.744934521267556	27.86707918344148	21.661106681149352
66-67	21.80676918289242	28.58582068338166	27.83364004416636	21.773770089559562
68-69	21.80864700054575	28.34821231435451	28.018384920696853	21.824755764402887
70-71	21.92443870012692	27.873276852620933	28.169195416028092	22.03308903122406
72-73	22.078924242695976	27.673651330009108	28.19423890504688	22.053185522248036
74-75	21.9282532203494	27.8679447143458	28.128132152778022	22.07566991252678
76-77	22.054085683327173	27.777310586348293	28.106353719639888	22.062250010684647
78-79	22.00822816905802	27.790296705346563	28.06910033773206	22.132374787863355
80-81	22.01148523916469	27.78349524944334	28.117021895388888	22.087997616003086
82-83	22.077542497521037	27.77864706013407	28.144335455881915	21.99947498646298
84-85	22.186181799702453	27.750396366103836	28.043376638522254	22.020045195671457
86-87	22.0213774298272	27.745160333353674	28.18842167219661	22.045040564622514
88-89	22.09283715052671	27.77251393737556	28.063632008277224	22.07101690382051
90-91	22.119649050622982	27.826787476393317	28.056256130496394	21.997307342487314
92-93	22.145121947170917	27.79486671520872	28.054553371675095	22.00545796594527
94-95	22.04877659361059	27.87035859173764	28.02442691144882	22.056437903202948
96-97	22.104461843447233	27.871794182296618	28.03280410930157	21.990939864954587
98-99	22.3282210041744	28.45287234292573	28.081221792879056	21.137684860020816
100-101	22.909426724692818	30.960889315631846	25.52018182474942	20.60950213492592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1456.0
1	1469.0
2	2248.0
3	3292.0
4	4149.5
5	6002.5
6	8019.5
7	10265.5
8	13295.5
9	15064.0
10	14281.0
11	12996.0
12	13521.0
13	16548.0
14	22792.5
15	30188.0
16	36481.0
17	41226.5
18	42981.5
19	41435.5
20	38349.0
21	35354.5
22	34816.0
23	38713.0
24	46819.0
25	59736.0
26	76723.5
27	95169.5
28	114901.5
29	135842.5
30	155910.0
31	176664.0
32	198609.0
33	220612.0
34	242577.5
35	262999.5
36	285085.0
37	306176.0
38	322818.5
39	336491.0
40	349221.0
41	362877.5
42	371976.0
43	383221.0
44	417156.5
45	439303.5
46	427234.5
47	410292.0
48	394753.0
49	381303.0
50	370911.0
51	358212.5
52	340570.0
53	321136.5
54	301435.0
55	278773.5
56	254191.0
57	229043.5
58	203602.0
59	176476.5
60	146618.5
61	118811.5
62	93780.0
63	72481.5
64	55136.0
65	41154.5
66	30489.0
67	22840.0
68	17178.0
69	12419.0
70	9074.0
71	6533.0
72	4552.0
73	3129.0
74	2059.0
75	1306.5
76	848.5
77	544.5
78	345.0
79	221.5
80	130.5
81	71.5
82	56.0
83	46.5
84	32.0
85	19.0
86	12.5
87	11.0
88	11.0
89	9.0
90	6.5
91	3.0
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.04469512425784954
6	0.0
7	0.0018755421985868583
8	0.0
9	0.0
10-11	0.0
12-13	1.0596283607835358E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.770170027940475E-5
28-29	2.651313932856217E-5
30-31	2.1234563732968354E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.0986743614889147E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	7.647314900283263E-4
54-55	1.6538271699281754E-4
56-57	0.003176642520928358
58-59	0.00790024873918686
60-61	0.0034823419887322613
62-63	0.005796115579717664
64-65	0.006683505387183822
66-67	0.0051105885890011995
68-69	0.00393176298316528
70-71	0.005366142553014827
72-73	0.003469226630228862
74-75	0.0032849610866127676
76-77	0.002532825703944854
78-79	1.3314992459592963E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.9449500628496294E-5
88-89	6.6383465949796045E-6
90-91	2.2128075827685588E-4
92-93	0.0
94-95	1.643173620052524E-4
96-97	6.930523237178099E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	148.0
20-21	523.0
22-23	1164.0
24-25	1299.0
26-27	3248.0
28-29	7703.0
30-31	19048.0
32-33	24170.0
34-35	32948.0
36-37	49759.0
38-39	57455.0
40-41	56291.0
42-43	63503.0
44-45	74977.0
46-47	74842.0
48-49	68143.0
50-51	61005.0
52-53	58077.0
54-55	62694.0
56-57	65009.0
58-59	65824.0
60-61	67257.0
62-63	65534.0
64-65	82584.0
66-67	88367.0
68-69	82052.0
70-71	101991.0
72-73	64395.0
74-75	64869.0
76-77	68710.0
78-79	71734.0
80-81	72036.0
82-83	68395.0
84-85	69269.0
86-87	71703.0
88-89	74780.0
90-91	77243.0
92-93	75518.0
94-95	83071.0
96-97	136220.0
98-99	780987.0
100-101	6322726.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.93400278828541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.32607514639656	77.6208119348532
2	6.8302498816972275	11.739014247569111
3	1.5970764319788369	4.117295116763228
4	0.5799970152372695	1.9936586049838696
5	0.25454749390649084	1.0937142525555725
6	0.13651656866913292	0.7038849115596255
7	0.07594675315495653	0.45684859481654805
8	0.0522923935423709	0.35949557539809746
9	0.03628884969624161	0.2806601499882448
>10	0.10854720906588035	1.4354775836258884
>50	0.0020332361556317535	0.1176468462864895
>100	3.896574999172876E-4	0.056968282878337835
>500	3.936299951044944E-5	0.024523898721810718
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1192567215670717E-5	0.0	0.0	0.0
3	0.0	2.1192567215670717E-5	0.0	0.0	0.0
4	0.0	5.2981418039176795E-5	0.0	0.0	0.0
5	0.0	7.417398525484751E-5	0.0	0.0	0.0
6	4.238513443134143E-5	7.417398525484751E-5	0.0	0.0	0.0
7	4.238513443134143E-5	7.417398525484751E-5	0.0	0.0	0.0
8	4.238513443134143E-5	8.477026886268287E-5	0.0	0.0	0.0
9	4.238513443134143E-5	1.4834797050969502E-4	0.0	0.0	0.0
10-11	4.238513443134143E-5	1.4834797050969502E-4	0.0	1.0596283607835358E-5	0.0
12-13	4.238513443134143E-5	1.4834797050969502E-4	0.0	1.0596283607835358E-5	0.0
14-15	5.2981418039176795E-5	1.9073310494103644E-4	0.0	1.0596283607835358E-5	0.0
16-17	5.2981418039176795E-5	1.9073310494103644E-4	0.0	1.0596283607835358E-5	0.0
18-19	5.2981418039176795E-5	2.2252195576454253E-4	0.0	1.0596283607835358E-5	0.0
20-21	5.827955984309448E-5	2.4371452298021324E-4	0.0	1.0596283607835358E-5	0.0
22-23	6.357770164701216E-5	2.7020523199980167E-4	0.0	1.589442541175304E-5	0.0
24-25	6.357770164701216E-5	3.443792172546492E-4	0.0	2.1192567215670717E-5	0.0
26-27	6.357770164701216E-5	3.7086992627423756E-4	0.0	5.827955984309448E-5	5.298141803917679E-6
28-29	6.357770164701216E-5	3.761680680781552E-4	0.0	2.6490709019588395E-4	1.0596283607835358E-5
30-31	6.357770164701216E-5	4.026587770977436E-4	0.0	9.642618083130175E-4	1.0596283607835358E-5
32-33	6.357770164701216E-5	4.026587770977436E-4	0.0	0.0028239095814881227	1.0596283607835358E-5
34-35	6.357770164701216E-5	4.8213090415650877E-4	0.0	0.004943166303055194	2.1192567215670717E-5
36-37	6.357770164701216E-5	5.61603031215274E-4	0.0	0.008000194123915696	2.1192567215670717E-5
38-39	6.357770164701216E-5	5.721993148231093E-4	0.0	0.01280560874006903	2.1192567215670717E-5
40-41	6.357770164701216E-5	6.622677254897099E-4	0.0	0.0178017564611634	2.1192567215670717E-5
42-43	6.357770164701216E-5	6.834602927053807E-4	0.0	0.02214093459857198	2.6490709019588397E-5
44-45	6.357770164701216E-5	7.523361361563104E-4	0.0	0.027677492783665956	3.178885082350608E-5
46-47	6.357770164701216E-5	8.053175541954872E-4	0.0	0.032996827154799305	3.7086992627423756E-5
48-49	6.357770164701216E-5	8.053175541954872E-4	0.0	0.03848570206365802	4.768327623525911E-5
50-51	9.006841066660054E-5	8.953859648620877E-4	0.0	0.04417060821926169	5.2981418039176795E-5
52-53	9.536655247051822E-5	9.112803902738408E-4	0.0	0.04927271877643441	5.2981418039176795E-5
54-55	1.0066469427443591E-4	9.165785320777585E-4	0.0	0.05477218996890097	5.2981418039176795E-5
56-57	1.0596283607835359E-4	9.324729574895116E-4	0.0	0.06067961808026918	5.827955984309448E-5
58-59	1.0596283607835359E-4	9.589636665090999E-4	0.0	0.06585590262269675	6.357770164701216E-5
60-61	1.0596283607835359E-4	9.801562337247707E-4	0.0	0.07079377078394802	6.357770164701216E-5
62-63	1.0596283607835359E-4	9.907525173326061E-4	0.0	0.07540845229516033	6.357770164701216E-5
64-65	1.0596283607835359E-4	0.0011126097788227126	0.0	0.08020856876950974	7.417398525484751E-5
66-67	1.0596283607835359E-4	0.0011179079206266303	0.0	0.08533187189389814	7.417398525484751E-5
68-69	1.0596283607835359E-4	0.0011496967714501364	0.0	0.09029623076416901	7.417398525484751E-5
70-71	1.1126097788227127E-4	0.001154994913254054	0.0	0.09522880078361637	7.417398525484751E-5
72-73	1.3775168690185967E-4	0.0011602930550579718	0.0	0.09894809632996657	7.417398525484751E-5
74-75	1.3775168690185967E-4	0.0011814856222736423	0.0	0.10190445945655265	7.417398525484751E-5
76-77	1.3775168690185967E-4	0.001223870756704984	0.0	0.10447935637325663	7.417398525484751E-5
78-79	1.3775168690185967E-4	0.001239765182116737	0.0	0.106837029476	7.417398525484751E-5
80-81	1.3775168690185967E-4	0.001239765182116737	0.0	0.10786486898596004	7.417398525484751E-5
82-83	1.3775168690185967E-4	0.001239765182116737	0.0	0.10838408688274397	7.417398525484751E-5
84-85	1.3775168690185967E-4	0.0012556596075284899	0.0	0.10871257167458687	7.417398525484751E-5
86-87	1.3775168690185967E-4	0.0013033428837637491	0.0	0.10879204380164562	7.417398525484751E-5
88-89	1.3775168690185967E-4	0.0013669205854107613	0.0	0.1088132363688613	7.417398525484751E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13295	0.0	48.212116	1
CTTATAC	9730	0.0	44.14004	1
GTATCAA	23455	0.0	37.654476	1
TATACAC	14410	0.0	34.87695	3
TCAACGC	27100	0.0	32.71756	4
TTATACA	14440	0.0	32.67788	2
ATCAACG	27370	0.0	32.331596	3
CAACGCA	27570	0.0	32.128433	5
TATCAAC	28085	0.0	31.292885	2
AACGCAG	28580	0.0	31.116863	6
ACGCAGA	33205	0.0	26.704588	7
CGCAGAG	33890	0.0	26.16427	8
TACACAT	19935	0.0	25.102297	5
GTGGTAT	6570	0.0	24.818316	1
ACACATC	19100	0.0	24.582169	6
ACATCTC	19560	0.0	23.893038	8
TGGTATC	6435	0.0	23.322617	2
GCAGAGT	40225	0.0	22.129663	9
CACATCT	21475	0.0	21.662214	7
ATACACA	25320	0.0	21.300957	4
>>END_MODULE