FastQCFastQC Report
Fri 27 May 2016
SRR2139680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9191035
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117950.049.3934021
GTATCAA215300.038.4596521
CTTATAC78200.037.7529561
TCAACGC252100.032.5856934
ATCAACG253550.032.382123
CAACGCA257300.031.8083275
AACGCAG265900.030.8331686
TATCAAC267000.030.5546042
TATACAC119300.029.505683
TTATACA121500.026.5631282
ACGCAGA313900.026.0626857
CGCAGAG316000.025.9027358
TGGTATC60000.021.9819742
GTGGTAT67550.021.7232591
ACACATC150150.021.486226
GCAGAGT382300.021.4105789
ACATCTC151300.021.3512958
TACACAT167550.020.9306225
GAGTACT232250.020.26256812-13
GTACATG253100.020.0332371
Produced by FastQC (version 0.11.5)