##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9191035
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.294197334685375	30.0	18.0	32.0	18.0	33.0
2	27.720624826257325	29.0	25.0	32.0	18.0	33.0
3	27.35632483175181	29.0	25.0	31.0	18.0	33.0
4	30.70763336229271	32.0	30.0	33.0	27.0	33.0
5	31.22327398383316	33.0	32.0	33.0	28.0	33.0
6	35.10760050418696	37.0	35.0	38.0	29.0	38.0
7	35.63848641638292	38.0	36.0	38.0	31.0	38.0
8	36.103753385772116	38.0	37.0	38.0	33.0	38.0
9	36.58224508991643	38.0	37.0	38.0	34.0	38.0
10-11	36.67717128702046	38.0	38.0	38.0	34.0	38.0
12-13	36.77399596454589	38.0	38.0	38.0	34.0	38.0
14-15	36.774257034164265	38.0	38.0	38.0	34.0	38.0
16-17	36.79416942705582	38.0	38.0	38.0	35.0	38.0
18-19	36.806219593331974	38.0	38.0	38.0	35.0	38.0
20-21	36.84530873528435	38.0	38.0	38.0	35.0	38.0
22-23	36.832142062375155	38.0	38.0	38.0	35.0	38.0
24-25	36.83525964752351	38.0	38.0	38.0	35.0	38.0
26-27	36.79058210146107	38.0	38.0	38.0	35.0	38.0
28-29	36.71158552887943	38.0	38.0	38.0	34.0	38.0
30-31	36.74410449059671	38.0	38.0	38.0	35.0	38.0
32-33	36.74242688060512	38.0	38.0	38.0	35.0	38.0
34-35	36.72359674477359	38.0	38.0	38.0	34.0	38.0
36-37	36.69544298001898	38.0	38.0	38.0	34.0	38.0
38-39	36.67122109351599	38.0	38.0	38.0	34.0	38.0
40-41	36.69347523357084	38.0	38.0	38.0	34.0	38.0
42-43	36.66319917048354	38.0	38.0	38.0	34.0	38.0
44-45	36.6053527153505	38.0	38.0	38.0	34.0	38.0
46-47	36.489654328457505	38.0	38.0	38.0	34.0	38.0
48-49	36.4411594168042	38.0	38.0	38.0	34.0	38.0
50-51	36.450562115631755	38.0	38.0	38.0	34.0	38.0
52-53	36.4587717775498	38.0	38.0	38.0	34.0	38.0
54-55	36.38492291382369	38.0	38.0	38.0	34.0	38.0
56-57	36.399206488293984	38.0	38.0	38.0	34.0	38.0
58-59	36.3410322361853	38.0	38.0	38.0	33.5	38.0
60-61	36.35053256768366	38.0	38.0	38.0	33.0	38.0
62-63	36.369278694952236	38.0	38.0	38.0	34.0	38.0
64-65	36.3612923055652	38.0	38.0	38.0	34.0	38.0
66-67	36.339226107783674	38.0	38.0	38.0	33.0	38.0
68-69	36.31366239259664	38.0	38.0	38.0	33.0	38.0
70-71	36.31220975916695	38.0	38.0	38.0	33.0	38.0
72-73	36.30385593656611	38.0	38.0	38.0	33.0	38.0
74-75	36.2712062701752	38.0	37.5	38.0	33.0	38.0
76-77	36.26610153684919	38.0	37.0	38.0	33.0	38.0
78-79	36.254733900961874	38.0	37.0	38.0	33.0	38.0
80-81	36.259632531046805	38.0	37.0	38.0	33.0	38.0
82-83	36.23016374464014	38.0	37.0	38.0	33.0	38.0
84-85	36.203073353977786	38.0	37.0	38.0	33.0	38.0
86-87	36.207828327244584	38.0	37.0	38.0	33.0	38.0
88-89	36.21230605015114	38.0	37.0	38.0	33.0	38.0
90-91	36.15752764017496	38.0	37.0	38.0	33.0	38.0
92-93	36.17832231482404	38.0	37.0	38.0	33.0	38.0
94-95	36.135725992893725	38.0	37.0	38.0	33.0	38.0
96-97	36.1688393642751	38.0	37.0	38.0	33.0	38.0
98-99	36.21542516866741	38.0	37.0	38.0	33.0	38.0
100-101	35.67678574957943	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	25.0
19	85.0
20	208.0
21	672.0
22	2202.0
23	6251.0
24	13895.0
25	25927.0
26	43393.0
27	65771.0
28	93174.0
29	124145.0
30	161076.0
31	204447.0
32	262286.0
33	345401.0
34	491813.0
35	812988.0
36	1924278.0
37	4612997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26907633362293	19.14967139174206	13.133373988892439	21.447878285742576
2	14.7693486098138	20.79054208802382	40.35486754212121	24.08524176004117
3	20.05177871697801	25.95371467957635	29.231735054865965	24.762771548579675
4	13.130392823006332	18.65995505402819	38.71932812789854	29.49032399506693
5	13.986242725879134	38.11273049116188	34.50292913272005	13.398097650238944
6	29.745213678328934	37.796352641459855	18.77710181715117	13.681331863060036
7	26.53455517530911	32.9795275134773	22.48248062224678	18.003436688966808
8	23.93850094140649	37.19580003775418	21.037521889536922	17.828177131302407
9	24.992310441642317	17.831277979030652	21.323909657617452	35.85250192170958
10-11	23.969803183210598	27.10872605751148	28.57698289692075	20.344487862357177
12-13	24.941799264174275	24.41144550096915	28.8399511045274	21.806804130329173
14-15	22.136456884344362	25.911080743354802	26.419897215057937	25.5325651572429
16-17	21.694640483906326	29.23699561583652	27.12448054000447	21.94388336025268
18-19	21.674425132751644	28.097983524162405	28.962320348034797	21.265270995051154
20-21	22.322053612132965	27.190150399909474	29.050368682697865	21.4374273052597
22-23	22.036685990249328	27.357880864571687	28.883913077990304	21.721520067188678
24-25	22.016728928952652	27.426550580122576	29.019737084806096	21.536983406118672
26-27	21.984546943846922	27.532717831909515	28.947439452256845	21.535295771986718
28-29	21.751487771260916	27.351935108451013	29.087359289103638	21.809217831184434
30-31	21.958750818676183	27.2991081184448	29.134523169966492	21.607617892912522
32-33	21.566119151858445	27.288498170610094	29.302647913106533	21.842734764424932
34-35	21.706688952502766	27.373836964769826	29.086677508572684	21.832796574154724
36-37	21.786994707902117	27.393636301726467	29.130774570689816	21.688594419681596
38-39	21.67681655494752	27.42432567660019	29.16105633167298	21.737801436779307
40-41	21.91498598571607	27.25960385084675	29.127473962155804	21.69793620128138
42-43	21.96080573789506	27.27022473913395	28.98092027905548	21.788049243915506
44-45	21.835814524829505	27.507397178004044	28.836443011832845	21.820345285333605
46-47	22.1205223571878	27.38595493034328	28.387838633330077	22.10568407913884
48-49	21.993767289648876	27.44807860867408	28.459149684465334	22.099004417211713
50-51	21.859501459902976	27.615994474730737	28.542341260548454	21.982162804817833
52-53	21.988461521365625	27.609620944449777	28.596812541602734	21.80510499258186
54-55	21.96941984749936	27.564878917098067	28.390751284093813	22.074949951308763
56-57	22.08731765131261	27.88146712096352	28.117926635535024	21.913288592188845
58-59	21.859401028933707	28.113886144572675	28.353668995979632	21.673043830513986
60-61	22.06481990960372	28.295583679843723	28.04138191427321	21.598214496279354
62-63	21.813483744023408	28.51701725769365	28.007704689754085	21.661794308528854
64-65	21.81582556466576	28.43242214450551	27.993144876308108	21.758607414520625
66-67	21.87328553676391	28.33590926890926	27.949518932520668	21.841286261806168
68-69	21.902106626240624	28.165323434747503	28.085297422472856	21.847272516539025
70-71	21.969815983685464	27.778743139442142	28.20008801217726	22.051352864695136
72-73	22.081099316981607	27.69967470658225	28.174550652925618	22.044675323510525
74-75	21.96490488077493	27.80640700587566	28.134867020772752	22.093821092576658
76-77	22.10123862119941	27.745497836034044	28.056745681357942	22.096517861408604
78-79	22.024670333534104	27.741194881061208	28.088709044565995	22.145425740838693
80-81	22.02806108850491	27.7786433770927	28.079315329067388	22.113980205335
82-83	22.1190184121385	27.792112439757453	28.088712047835674	22.00015710026837
84-85	22.188231805877486	27.755108675365797	28.03796563114996	22.018693887606762
86-87	22.052228591791	27.785551332700464	28.100711046070526	22.06150902943801
88-89	22.11979232266859	27.753906731108422	28.06591272742387	22.06038821879912
90-91	22.167413317659225	27.793388007778624	28.017872451399338	22.021326223162813
92-93	22.16223824715903	27.780799372632586	28.0435845573949	22.01337782281348
94-95	22.068816889157358	27.873368740552202	28.020340770524584	22.03747359976586
96-97	22.11033123764786	27.862291695328327	28.03407519383571	21.993301873188102
98-99	22.34180718083054	28.3986951186732	28.121034763523156	21.138462936973106
100-101	22.95121003651736	30.964052326399994	25.47734132321864	20.607396313864008
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1369.0
1	1340.5
2	1963.0
3	2890.0
4	3665.0
5	5270.5
6	7054.0
7	9102.0
8	12081.5
9	14063.5
10	13323.0
11	12092.0
12	12755.0
13	15518.0
14	21255.5
15	28076.0
16	33952.0
17	38336.0
18	39447.0
19	37709.0
20	35108.0
21	32687.5
22	32456.0
23	35971.0
24	43608.5
25	55419.5
26	70491.5
27	87810.0
28	107358.5
29	127913.0
30	147558.0
31	167857.5
32	189721.0
33	211219.0
34	232420.0
35	252779.5
36	274257.5
37	295181.5
38	312601.0
39	327159.0
40	341013.5
41	352898.5
42	360583.0
43	367861.0
44	377357.0
45	396969.5
46	407626.5
47	400463.0
48	390486.5
49	376936.0
50	363330.5
51	349609.5
52	332778.0
53	312453.5
54	290825.5
55	268897.5
56	245651.5
57	219951.0
58	193969.5
59	167861.0
60	139364.5
61	112220.0
62	88452.5
63	67774.5
64	50449.5
65	37032.0
66	27436.5
67	20919.0
68	16066.0
69	11972.0
70	8734.0
71	6276.0
72	4390.0
73	2994.0
74	1976.5
75	1260.5
76	791.0
77	480.0
78	307.0
79	212.0
80	140.0
81	89.5
82	56.0
83	30.0
84	18.0
85	16.5
86	12.5
87	7.5
88	5.0
89	4.0
90	4.0
91	2.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.045544381019112644
6	0.0
7	0.002306595503117984
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.618663679860269E-5
28-29	3.8109229108003406E-5
30-31	3.269798959680763E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.617007489830127E-6
46-47	0.0
48-49	0.0
50-51	0.0
52-53	6.638497884570491E-4
54-55	1.509893548439737E-4
56-57	0.0029867062930310734
58-59	0.007484562648231263
60-61	0.003276665972819315
62-63	0.0056198001188629484
64-65	0.00636784722549292
66-67	0.004706979614780061
68-69	0.0036432484645938907
70-71	0.005258972612789068
72-73	0.003213778489819329
74-75	0.003073670486967261
76-77	0.0024140384297223487
78-79	7.643547737395217E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.607307347417828E-5
88-89	0.0
90-91	1.943178381832274E-4
92-93	0.0
94-95	8.194317309232026E-5
96-97	1.3812847246346744E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	110.0
20-21	465.0
22-23	934.0
24-25	1104.0
26-27	2876.0
28-29	6771.0
30-31	16215.0
32-33	20634.0
34-35	27773.0
36-37	42915.0
38-39	49363.0
40-41	48859.0
42-43	55690.0
44-45	65299.0
46-47	65600.0
48-49	59574.0
50-51	52722.0
52-53	50910.0
54-55	54470.0
56-57	57051.0
58-59	57834.0
60-61	58593.0
62-63	56733.0
64-65	68804.0
66-67	73243.0
68-69	70328.0
70-71	83847.0
72-73	57277.0
74-75	57802.0
76-77	61452.0
78-79	64130.0
80-81	64928.0
82-83	62088.0
84-85	62000.0
86-87	65027.0
88-89	68309.0
90-91	70135.0
92-93	68618.0
94-95	76923.0
96-97	130492.0
98-99	780840.0
100-101	6352297.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.25137892284228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.49409884727176	78.0524080995718
2	6.746676102225238	11.638202339254272
3	1.5304646774647412	3.9601406647211097
4	0.5822237386428688	2.008704011982399
5	0.24297962499300468	1.0478663852900882
6	0.13286154093531088	0.6875694666890532
7	0.0808940360145333	0.4884055507021293
8	0.05196806890828274	0.35858540826373386
9	0.03164874087290458	0.24567727873137643
>10	0.1040310335315042	1.3365854210076342
>50	0.0017543416251908262	0.0997053143393664
>100	3.796223210105673E-4	0.06384413028392064
>500	1.9625193748349835E-5	0.012305929163016061
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.088016746753766E-5	0.0
3	0.0	0.0	0.0	2.176033493507532E-5	0.0
4	0.0	3.264050240261298E-5	0.0	2.176033493507532E-5	0.0
5	0.0	3.264050240261298E-5	0.0	2.176033493507532E-5	0.0
6	0.0	4.352066987015064E-5	0.0	2.176033493507532E-5	0.0
7	0.0	4.352066987015064E-5	0.0	3.264050240261298E-5	0.0
8	0.0	4.352066987015064E-5	0.0	3.264050240261298E-5	0.0
9	0.0	5.44008373376883E-5	0.0	5.44008373376883E-5	0.0
10-11	0.0	5.44008373376883E-5	0.0	7.072108853899478E-5	0.0
12-13	0.0	5.44008373376883E-5	0.0	7.616117227276362E-5	0.0
14-15	0.0	8.704133974030128E-5	0.0	8.160125600653245E-5	0.0
16-17	0.0	8.704133974030128E-5	0.0	9.792150720783895E-5	0.0
18-19	0.0	9.792150720783893E-5	0.0	1.1424175840914544E-4	1.088016746753766E-5
20-21	0.0	1.088016746753766E-4	0.0	1.3056200961045192E-4	1.088016746753766E-5
22-23	0.0	1.632025120130649E-4	0.0	1.414421770779896E-4	1.088016746753766E-5
24-25	0.0	2.0672318188321554E-4	0.0	1.632025120130649E-4	1.088016746753766E-5
26-27	0.0	2.3936368428582853E-4	0.0	2.2304343308452205E-4	1.088016746753766E-5
28-29	0.0	2.3936368428582853E-4	0.0	4.5696703363658176E-4	1.088016746753766E-5
30-31	0.0	2.5024385175336617E-4	0.0	0.0011043369979550723	1.088016746753766E-5
32-33	0.0	2.5024385175336617E-4	0.0	0.0026874013644818022	1.088016746753766E-5
34-35	5.44008373376883E-6	2.8288435415597913E-4	0.0	0.004259585563540994	1.088016746753766E-5
36-37	1.088016746753766E-5	3.1552485655859215E-4	0.0	0.007017708016561791	1.088016746753766E-5
38-39	1.088016746753766E-5	3.264050240261298E-4	0.0	0.010874727383803892	2.176033493507532E-5
40-41	1.088016746753766E-5	3.6992569389628045E-4	0.0	0.015259434873221569	2.176033493507532E-5
42-43	1.088016746753766E-5	3.6992569389628045E-4	0.0	0.01915997491033382	2.176033493507532E-5
44-45	1.088016746753766E-5	4.0800628003266224E-4	0.0	0.024023409768323154	2.176033493507532E-5
46-47	1.088016746753766E-5	4.352066987015064E-4	0.0	0.0288324437889748	3.264050240261298E-5
48-49	1.088016746753766E-5	4.460868661690441E-4	0.0	0.03330419261813278	4.352066987015064E-5
50-51	1.088016746753766E-5	5.004877035067323E-4	0.0	0.03840155107667417	4.352066987015064E-5
52-53	1.088016746753766E-5	5.004877035067323E-4	0.0	0.04329762643706612	4.896075360391947E-5
54-55	1.088016746753766E-5	5.004877035067323E-4	0.0	0.04801961911797746	5.44008373376883E-5
56-57	1.088016746753766E-5	5.1136787097427E-4	0.0	0.05309521724158378	5.44008373376883E-5
58-59	1.088016746753766E-5	5.276881221755765E-4	0.0	0.05774104875022236	5.44008373376883E-5
60-61	1.088016746753766E-5	5.603286245781895E-4	0.0	0.06210943598843873	5.44008373376883E-5
62-63	1.088016746753766E-5	5.76648875779496E-4	0.0	0.06645606289172004	5.44008373376883E-5
64-65	1.088016746753766E-5	6.310497131171843E-4	0.0	0.07117805557263138	5.44008373376883E-5
66-67	1.088016746753766E-5	6.364897968509531E-4	0.0	0.07574228582526343	5.44008373376883E-5
68-69	1.088016746753766E-5	6.800104667211038E-4	0.0	0.08035547683149939	5.44008373376883E-5
70-71	1.088016746753766E-5	6.963307179224102E-4	0.0	0.08441377929689094	5.44008373376883E-5
72-73	1.088016746753766E-5	7.01770801656179E-4	0.0	0.08742758568539888	6.528100480522596E-5
74-75	1.088016746753766E-5	7.235311365912544E-4	0.0	0.09022378872455605	6.528100480522596E-5
76-77	1.088016746753766E-5	7.507315552600986E-4	0.0	0.0928350289167651	6.528100480522596E-5
78-79	1.088016746753766E-5	7.616117227276362E-4	0.0	0.09491858098679856	6.528100480522596E-5
80-81	1.088016746753766E-5	7.724918901951739E-4	0.0	0.09583251505407171	7.616117227276362E-5
82-83	1.088016746753766E-5	7.724918901951739E-4	0.0	0.09636020317624729	9.792150720783895E-5
84-85	1.088016746753766E-5	7.833720576627116E-4	0.0	0.09671380861894226	9.792150720783895E-5
86-87	1.088016746753766E-5	8.051323925977868E-4	0.0	0.09683349046108516	9.792150720783895E-5
88-89	1.088016746753766E-5	8.268927275328622E-4	0.0	0.09688789129842286	1.0336159094160777E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11795	0.0	49.393402	1
GTATCAA	21530	0.0	38.459652	1
CTTATAC	7820	0.0	37.752956	1
TCAACGC	25210	0.0	32.585693	4
ATCAACG	25355	0.0	32.38212	3
CAACGCA	25730	0.0	31.808327	5
AACGCAG	26590	0.0	30.833168	6
TATCAAC	26700	0.0	30.554604	2
TATACAC	11930	0.0	29.50568	3
TTATACA	12150	0.0	26.563128	2
ACGCAGA	31390	0.0	26.062685	7
CGCAGAG	31600	0.0	25.902735	8
TGGTATC	6000	0.0	21.981974	2
GTGGTAT	6755	0.0	21.723259	1
ACACATC	15015	0.0	21.48622	6
GCAGAGT	38230	0.0	21.410578	9
ACATCTC	15130	0.0	21.351295	8
TACACAT	16755	0.0	20.930622	5
GAGTACT	23225	0.0	20.262568	12-13
GTACATG	25310	0.0	20.033237	1
>>END_MODULE