FastQCFastQC Report
Fri 27 May 2016
SRR2139679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7640430
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121750.049.28581
CTTATAC68650.035.7403681
GTATCAA205300.035.08661
TCAACGC235750.030.5176354
TGGTATC54300.030.2042522
ATCAACG240250.029.8550033
GTGGTAT60100.029.6908321
CAACGCA243050.029.5470565
TATCAAC242300.029.2053092
AACGCAG249500.028.805076
TATACAC107450.027.8003943
TTATACA104700.026.1495572
ACGCAGA288250.024.9479167
CGCAGAG292100.024.5737118
TACACAT142200.021.345025
GAGTACT202150.021.3005912-13
GCGGTAT6650.021.045781
GCAGAGT346700.020.8045969
ACATCTC137500.020.4426638
ACACATC135200.020.2734936
Produced by FastQC (version 0.11.5)