##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7640430
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.20381326705434	30.0	18.0	32.0	18.0	33.0
2	27.546373437097127	29.0	25.0	32.0	18.0	33.0
3	27.203753977197618	29.0	25.0	31.0	18.0	33.0
4	30.582415125850247	32.0	30.0	33.0	27.0	33.0
5	31.124581993421835	33.0	32.0	33.0	28.0	33.0
6	35.03223431141964	37.0	34.0	38.0	29.0	38.0
7	35.57207408483554	38.0	36.0	38.0	31.0	38.0
8	36.02960618708633	38.0	36.0	38.0	32.0	38.0
9	36.511082229665085	38.0	37.0	38.0	34.0	38.0
10-11	36.60118121624045	38.0	38.0	38.0	34.0	38.0
12-13	36.704306825662954	38.0	38.0	38.0	34.0	38.0
14-15	36.70228769846723	38.0	38.0	38.0	34.0	38.0
16-17	36.717029146265325	38.0	38.0	38.0	34.0	38.0
18-19	36.730730011269	38.0	38.0	38.0	34.0	38.0
20-21	36.77438841696984	38.0	38.0	38.0	34.5	38.0
22-23	36.758954388217724	38.0	38.0	38.0	34.5	38.0
24-25	36.76241749004298	38.0	38.0	38.0	34.5	38.0
26-27	36.718970693707995	38.0	38.0	38.0	34.5	38.0
28-29	36.62835731607711	38.0	38.0	38.0	34.0	38.0
30-31	36.66811245234059	38.0	38.0	38.0	34.0	38.0
32-33	36.66259126841007	38.0	38.0	38.0	34.0	38.0
34-35	36.635811650849554	38.0	38.0	38.0	34.0	38.0
36-37	36.60608856243289	38.0	38.0	38.0	34.0	38.0
38-39	36.57665844958914	38.0	38.0	38.0	34.0	38.0
40-41	36.60690446763679	38.0	38.0	38.0	34.0	38.0
42-43	36.58061172914576	38.0	38.0	38.0	34.0	38.0
44-45	36.519628794344946	38.0	38.0	38.0	34.0	38.0
46-47	36.40437206337515	38.0	38.0	38.0	34.0	38.0
48-49	36.357441781695634	38.0	38.0	38.0	33.5	38.0
50-51	36.36717044957983	38.0	38.0	38.0	34.0	38.0
52-53	36.38497708309046	38.0	38.0	38.0	34.0	38.0
54-55	36.30538309768322	38.0	38.0	38.0	33.0	38.0
56-57	36.316291550372924	38.0	38.0	38.0	33.0	38.0
58-59	36.25515260285637	38.0	37.5	38.0	33.0	38.0
60-61	36.26411274802246	38.0	37.0	38.0	33.0	38.0
62-63	36.2780106214652	38.0	37.0	38.0	33.0	38.0
64-65	36.26543807653929	38.0	37.0	38.0	33.0	38.0
66-67	36.24216180790134	38.0	37.0	38.0	33.0	38.0
68-69	36.212240077412346	38.0	37.0	38.0	33.0	38.0
70-71	36.20950361439064	38.0	37.0	38.0	33.0	38.0
72-73	36.19828904259792	38.0	37.0	38.0	33.0	38.0
74-75	36.16474576280136	38.0	37.0	38.0	33.0	38.0
76-77	36.15732868591775	38.0	37.0	38.0	33.0	38.0
78-79	36.14175371675833	38.0	37.0	38.0	33.0	38.0
80-81	36.14647257811801	38.0	37.0	38.0	33.0	38.0
82-83	36.11432689246071	38.0	37.0	38.0	33.0	38.0
84-85	36.086957975589044	38.0	37.0	38.0	33.0	38.0
86-87	36.08734115272637	38.0	37.0	38.0	33.0	38.0
88-89	36.08948133981161	38.0	37.0	38.0	33.0	38.0
90-91	36.03350126249888	38.0	37.0	38.0	32.5	38.0
92-93	36.05407637536524	38.0	37.0	38.0	33.0	38.0
94-95	36.01028785806862	38.0	37.0	38.0	31.5	38.0
96-97	36.04177923936486	38.0	37.0	38.0	32.5	38.0
98-99	36.0906842661895	38.0	37.0	38.0	33.0	38.0
100-101	35.5417602079509	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	5.0
18	28.0
19	130.0
20	302.0
21	833.0
22	2458.0
23	6392.0
24	13572.0
25	24679.0
26	40337.0
27	60177.0
28	83242.0
29	111024.0
30	141501.0
31	179524.0
32	228692.0
33	301427.0
34	431629.0
35	708841.0
36	1646895.0
37	3658741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.05020528949287	18.839803518911893	13.082994019970078	21.02699717162516
2	15.03623487159754	20.410120896336988	40.308097842660686	24.245546389404783
3	20.80478454746657	25.500593552980654	28.760501699511675	24.934120200041097
4	13.348528813168892	18.345498878989794	38.13806291007181	30.1679093977695
5	14.1525746089589	37.96396090865702	34.21246735459085	13.670997127793234
6	30.218364673192475	37.32806923170555	18.391530319628608	14.062035775473369
7	26.851960922898115	32.80434748464716	21.876960006701328	18.4667315857534
8	24.062127393353517	37.4394111326195	20.491503750443364	18.006957723583618
9	24.86663708717965	18.023998649290682	21.006684178770044	36.102680084759626
10-11	24.293148422274662	27.039662689141842	27.942969178436293	20.724219710147203
12-13	25.198810799915712	24.299915057136836	28.419140022223882	22.08213412072357
14-15	22.388556665004455	25.880428195795265	26.029025853256947	25.701989285943334
16-17	21.901967559417468	29.27150697015744	26.7170303242095	22.109495146215593
18-19	21.784120789013183	27.966246664127542	28.769061427170982	21.480571119688292
20-21	22.39266381829788	27.039562878901734	28.917805324693617	21.649967978106766
22-23	22.192166184331754	27.165182209191464	28.779362338381137	21.863289268095645
24-25	22.032644748852945	27.26519215078922	28.948323221627568	21.75383987873026
26-27	21.899743078783494	27.30832773242461	28.97761470726572	21.81431448152617
28-29	21.659823436871932	27.19132731961043	29.219023704750512	21.92982553876712
30-31	22.05140461726479	27.002041142483453	28.88460013367935	22.061954106572408
32-33	22.00350698011287	27.20587800362397	29.10028477782663	21.690330238436527
34-35	22.04860693687501	27.48708479914207	28.95663621473372	21.507672049249198
36-37	21.899797062964943	27.268732151137858	28.820568604403384	22.01090218149381
38-39	21.78126555792823	27.271198499106575	29.04915255948342	21.89838338348178
40-41	22.021520058784784	27.08947093562262	29.018596976664345	21.870412028928243
42-43	22.009967240065627	27.121820339078372	28.95846522462464	21.909747196231354
44-45	21.928442972678404	27.342375821765863	28.769163732891883	21.96001747266385
46-47	22.24882951595285	27.223878887806052	28.2560959048546	22.27119569138649
48-49	22.0413698560724	27.323127362815473	28.39403527968956	22.24146750142257
50-51	21.943416744149	27.550388321190418	28.420781223105983	22.0854137115546
52-53	22.112463511913607	27.71082293259325	28.240709805184334	21.93600375030881
54-55	22.095347649852283	27.68934247766136	27.976035464134956	22.2392744083514
56-57	22.20819598676184	28.031690618052014	27.705526137501792	22.054587257684357
58-59	21.95428856486512	28.332069689709904	27.91290145221813	21.800740293206843
60-61	22.15160972103861	28.52570747779712	27.604657293854352	21.718025507309925
62-63	21.936519526173946	28.709393896703943	27.571003110847936	21.78308346627417
64-65	21.93534823133493	28.64078553712351	27.568467542263225	21.85539868927834
66-67	22.034737791296468	28.497757324930767	27.515911411785442	21.951593471987323
68-69	22.01811279869503	28.248577814180457	27.6922326248773	22.041076762247215
70-71	22.075122666156414	27.855947757012057	27.81983030585056	22.24909927098097
72-73	22.20709763144119	27.749350881151475	27.819989679955125	22.22356180745221
74-75	22.06857337943401	27.8935500983298	27.77884329560697	22.259033226629214
76-77	22.233381837460737	27.834929722108136	27.70160594549107	22.23008249494006
78-79	22.15844974349104	27.80555121098222	27.697619020149	22.33838002537774
80-81	22.155155744164144	27.852772254361614	27.72940505049315	22.262666950981096
82-83	22.20317709464367	27.862218794185495	27.802348881246004	22.132255229924837
84-85	22.27509184508242	27.85023123607722	27.705286963154773	22.169389955685585
86-87	22.144963151157462	27.86077232249497	27.797760896296964	22.196503630050604
88-89	22.242125689215904	27.869901803885288	27.689388575078116	22.198583931820693
90-91	22.265391763722675	27.90362468419852	27.678405649572003	22.152577902506803
92-93	22.23261339795167	27.871802549758335	27.719162271717007	22.176421780572987
94-95	22.163793545429105	27.98254574357993	27.701059978127073	22.152600732863895
96-97	22.23443990558333	27.939393909388578	27.697444171760715	22.12872201326738
98-99	22.442450083940145	28.544405040791187	27.751725728830273	21.261419146438392
100-101	23.061306872698825	31.027345116316717	25.205377016478103	20.705970994506355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2098.0
1	1732.0
2	1517.5
3	2062.5
4	2931.5
5	4235.5
6	5338.5
7	5705.0
8	5875.0
9	6063.0
10	6090.0
11	6911.5
12	8629.0
13	12392.5
14	18807.0
15	27031.5
16	34445.0
17	38734.5
18	40775.0
19	38165.0
20	34234.0
21	31819.5
22	30624.0
23	32856.0
24	38928.0
25	48931.0
26	61236.5
27	74858.0
28	90042.0
29	105993.0
30	121413.5
31	137167.5
32	153690.5
33	170530.5
34	186558.0
35	201365.5
36	218445.5
37	234389.5
38	247768.5
39	259215.0
40	269759.5
41	280827.5
42	287959.5
43	293203.5
44	301624.0
45	319294.5
46	329675.5
47	327430.0
48	324454.5
49	314492.5
50	302356.5
51	291546.5
52	278427.5
53	264209.0
54	249701.0
55	233308.5
56	214754.5
57	194253.5
58	173168.5
59	150818.0
60	126311.5
61	102580.5
62	80730.5
63	61945.0
64	46562.0
65	34489.0
66	25450.5
67	19174.0
68	14383.5
69	10262.5
70	7337.0
71	5264.5
72	3618.0
73	2365.5
74	1572.5
75	1019.0
76	641.0
77	384.0
78	233.0
79	152.5
80	102.5
81	73.5
82	49.5
83	30.0
84	22.5
85	20.0
86	16.0
87	13.0
88	9.0
89	4.5
90	1.5
91	0.0
92	0.0
93	0.5
94	1.5
95	2.0
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.045089085300172894
6	0.0
7	0.0019632402888319113
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.930460716813843E-5
28-29	2.621633470342083E-5
30-31	2.6242503828781305E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	7.926650392928927E-4
54-55	2.0274287337826677E-4
56-57	0.0030241177611148107
58-59	0.007862944420023629
60-61	0.003371059744301199
62-63	0.005626150750556497
64-65	0.006566904146822972
66-67	0.005054686021695528
68-69	0.0036887950279158015
70-71	0.005252631935838952
72-73	0.003270277102477613
74-75	0.00283990520290972
76-77	0.002343615967351382
78-79	9.932869086313576E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	7.090553059199421E-5
88-89	0.0
90-91	2.724577944846048E-4
92-93	0.0
94-95	1.224595061110151E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	814.0
20-21	2907.0
22-23	1763.0
24-25	1691.0
26-27	3123.0
28-29	5814.0
30-31	13002.0
32-33	15999.0
34-35	22290.0
36-37	34654.0
38-39	39915.0
40-41	38408.0
42-43	43017.0
44-45	51083.0
46-47	51970.0
48-49	46977.0
50-51	66626.0
52-53	38319.0
54-55	41594.0
56-57	44158.0
58-59	47106.0
60-61	49833.0
62-63	46102.0
64-65	55692.0
66-67	59486.0
68-69	57541.0
70-71	67972.0
72-73	44206.0
74-75	44411.0
76-77	47350.0
78-79	50813.0
80-81	51334.0
82-83	47785.0
84-85	47931.0
86-87	50307.0
88-89	54583.0
90-91	58687.0
92-93	55454.0
94-95	61182.0
96-97	105650.0
98-99	651296.0
100-101	5321585.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.13782295963908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.48458188726963	77.94144895182596
2	6.768877264109169	11.661127028227233
3	1.5352739690388724	3.9673547201883834
4	0.5624370191361187	1.937884015211765
5	0.24497186068203275	1.0550671382761152
6	0.12942001908307263	0.6688775214726492
7	0.07320177774784867	0.4413809240382525
8	0.05232686343748108	0.36058576790487645
9	0.036702696184046785	0.2845341311438558
>10	0.10911296075112706	1.4373229743185962
>50	0.00256033090797651	0.14535088482041028
>100	4.853244390616499E-4	0.06396391693738057
>500	2.4013750132595832E-5	0.011128487807449792
>1k	2.401346337725242E-5	0.023973537827065156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.235307436885097E-5	0.0	1.3088268592212742E-5	1.3088268592212742E-5
5	0.0	5.235307436885097E-5	0.0	1.3088268592212742E-5	1.3088268592212742E-5
6	0.0	5.235307436885097E-5	0.0	1.3088268592212742E-5	1.3088268592212742E-5
7	0.0	5.235307436885097E-5	0.0	1.3088268592212742E-5	1.3088268592212742E-5
8	0.0	6.544134296106371E-5	0.0	1.3088268592212742E-5	1.3088268592212742E-5
9	0.0	1.4397095451434017E-4	0.0	1.3088268592212742E-5	1.3088268592212742E-5
10-11	0.0	1.4397095451434017E-4	0.0	1.3088268592212742E-5	1.3088268592212742E-5
12-13	0.0	1.4397095451434017E-4	0.0	1.3088268592212742E-5	1.3088268592212742E-5
14-15	0.0	2.552212375481485E-4	0.0	1.3088268592212742E-5	2.6176537184425484E-5
16-17	0.0	2.6176537184425484E-4	0.0	1.3088268592212742E-5	3.9264805776638225E-5
18-19	0.0	2.6176537184425484E-4	0.0	2.6176537184425484E-5	6.544134296106371E-5
20-21	0.0	2.7485364043646756E-4	0.0	5.235307436885097E-5	9.816201444159557E-5
22-23	0.0	3.141184462131058E-4	0.0	5.235307436885097E-5	1.4397095451434017E-4
24-25	0.0	3.337508491014249E-4	0.0	7.198547725717009E-5	1.9632402888319114E-4
26-27	0.0	3.5338325198974404E-4	0.0	9.161788014548919E-5	1.9632402888319114E-4
28-29	0.0	3.5338325198974404E-4	0.0	3.795597891741695E-4	2.028681631792975E-4
30-31	0.0	3.795597891741695E-4	0.0	0.001020884950192594	2.1595643177151026E-4
32-33	0.0	3.795597891741695E-4	0.0	0.0023755207494866124	2.3558883465982935E-4
34-35	0.0	4.3845699783912686E-4	0.0	0.004155525278027546	2.6176537184425484E-4
36-37	0.0	5.038983408001906E-4	0.0	0.00685170860802337	2.6176537184425484E-4
38-39	0.0	5.824279523534671E-4	0.0	0.01090907187160932	2.6176537184425484E-4
40-41	0.0	6.216927581301052E-4	0.0	0.014959891000899162	2.6176537184425484E-4
42-43	0.0	6.544134296106372E-4	0.0	0.01883401850419414	2.6176537184425484E-4
44-45	0.0	7.394871754600199E-4	0.0	0.023238220885473726	2.6830950614036117E-4
46-47	0.0	8.049285184210836E-4	0.0	0.028048159593111903	2.8139777473257395E-4
48-49	0.0	8.1147265271719E-4	0.0	0.03263559773468247	2.8794190902868035E-4
50-51	1.3088268592212742E-5	9.030905328626792E-4	0.0	0.03704634425025817	2.8794190902868035E-4
52-53	1.3088268592212742E-5	9.030905328626792E-4	0.0	0.0411822371253974	3.141184462131058E-4
54-55	1.3088268592212742E-5	9.16178801454892E-4	0.0	0.04584166074422513	3.402949833975313E-4
56-57	1.3088268592212742E-5	9.423553386393174E-4	0.0	0.05034402513994631	3.402949833975313E-4
58-59	1.3088268592212742E-5	9.423553386393174E-4	0.0	0.055023081161662366	3.402949833975313E-4
60-61	1.3088268592212742E-5	9.88164278712062E-4	0.0	0.05972831372056285	3.402949833975313E-4
62-63	1.3088268592212742E-5	0.0010143408158964874	0.0	0.06406053062458526	3.6647152058195677E-4
64-65	1.3088268592212742E-5	0.0011386793675225086	0.0	0.06818987936542839	3.6647152058195677E-4
66-67	1.3088268592212742E-5	0.0011386793675225086	0.0	0.07263989068678071	3.991921920624886E-4
68-69	1.3088268592212742E-5	0.001145223501818615	0.0	0.07696556345650703	4.3191286354302046E-4
70-71	1.3088268592212742E-5	0.0011779441732991467	0.0	0.081147265271719	4.4500113213523325E-4
72-73	2.6176537184425484E-5	0.0011910324418913595	0.0	0.0842360966594812	4.58089400727446E-4
74-75	2.6176537184425484E-5	0.0012172089790757849	0.0	0.08657235260319118	4.58089400727446E-4
76-77	2.6176537184425484E-5	0.0012499296505563168	0.0	0.08892824094978947	4.58089400727446E-4
78-79	2.6176537184425484E-5	0.0012564737848524232	0.0	0.09093729017869413	4.58089400727446E-4
80-81	2.6176537184425484E-5	0.0012564737848524232	0.0	0.09196471926318284	4.6463353502355237E-4
82-83	2.6176537184425484E-5	0.0012630179191485296	0.0	0.09228538184369205	4.8426593791187143E-4
84-85	2.6176537184425484E-5	0.001269562053444636	0.0	0.09251442654405576	4.908100722079778E-4
86-87	2.6176537184425484E-5	0.001269562053444636	0.0	0.09270420643864286	4.973542065040842E-4
88-89	2.6176537184425484E-5	0.001321915127813487	0.0	0.09278273605019613	4.973542065040842E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12175	0.0	49.2858	1
CTTATAC	6865	0.0	35.740368	1
GTATCAA	20530	0.0	35.0866	1
TCAACGC	23575	0.0	30.517635	4
TGGTATC	5430	0.0	30.204252	2
ATCAACG	24025	0.0	29.855003	3
GTGGTAT	6010	0.0	29.690832	1
CAACGCA	24305	0.0	29.547056	5
TATCAAC	24230	0.0	29.205309	2
AACGCAG	24950	0.0	28.80507	6
TATACAC	10745	0.0	27.800394	3
TTATACA	10470	0.0	26.149557	2
ACGCAGA	28825	0.0	24.947916	7
CGCAGAG	29210	0.0	24.573711	8
TACACAT	14220	0.0	21.34502	5
GAGTACT	20215	0.0	21.30059	12-13
GCGGTAT	665	0.0	21.04578	1
GCAGAGT	34670	0.0	20.804596	9
ACATCTC	13750	0.0	20.442663	8
ACACATC	13520	0.0	20.273493	6
>>END_MODULE