FastQCFastQC Report
Fri 27 May 2016
SRR2139678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9403026
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134450.051.464821
GTATCAA241900.038.1574171
TCAACGC281800.032.6929444
ATCAACG284050.032.3114133
CAACGCA291100.031.588685
TATCAAC292350.031.1856692
AACGCAG302450.030.4606116
CTTATAC74000.027.287291
TGGTATC58800.026.4959742
ACGCAGA354100.025.9806927
GTGGTAT66900.025.6297441
CGCAGAG361200.025.4697258
TATACAC114050.022.3602433
GCAGAGT419850.021.859999
GCGGTAT6300.021.4138771
GAGTACT249150.020.69675612-13
CAGAGTA405650.019.84263210-11
GTACATG299000.019.5323941
TACATGG291200.019.05432
TTATACA119650.018.876822