##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139678_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9403026
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.26839285566157	30.0	18.0	32.0	18.0	33.0
2	27.663744203195865	29.0	25.0	32.0	18.0	33.0
3	27.275001047535124	29.0	25.0	31.0	18.0	33.0
4	30.651038825161177	32.0	30.0	33.0	27.0	33.0
5	31.163679436811087	33.0	32.0	33.0	28.0	33.0
6	35.032654488033955	37.0	34.0	38.0	29.0	38.0
7	35.592422800915365	38.0	36.0	38.0	31.0	38.0
8	36.065331841047765	38.0	36.0	38.0	33.0	38.0
9	36.55842534094875	38.0	37.0	38.0	34.0	38.0
10-11	36.655305005005836	38.0	38.0	38.0	34.0	38.0
12-13	36.753765064565386	38.0	38.0	38.0	34.0	38.0
14-15	36.7569895052933	38.0	38.0	38.0	34.0	38.0
16-17	36.775096814578625	38.0	38.0	38.0	34.0	38.0
18-19	36.78884090079087	38.0	38.0	38.0	34.5	38.0
20-21	36.830085421777724	38.0	38.0	38.0	35.0	38.0
22-23	36.81678032581283	38.0	38.0	38.0	35.0	38.0
24-25	36.82310917221764	38.0	38.0	38.0	35.0	38.0
26-27	36.78169955007314	38.0	38.0	38.0	35.0	38.0
28-29	36.69778260237908	38.0	38.0	38.0	34.0	38.0
30-31	36.73207231027374	38.0	38.0	38.0	34.0	38.0
32-33	36.72818809078338	38.0	38.0	38.0	34.0	38.0
34-35	36.707842891834275	38.0	38.0	38.0	34.0	38.0
36-37	36.68294539156249	38.0	38.0	38.0	34.0	38.0
38-39	36.65992733398258	38.0	38.0	38.0	34.0	38.0
40-41	36.67794090041184	38.0	38.0	38.0	34.0	38.0
42-43	36.64511591598831	38.0	38.0	38.0	34.0	38.0
44-45	36.58595217456727	38.0	38.0	38.0	34.0	38.0
46-47	36.46786109244469	38.0	38.0	38.0	34.0	38.0
48-49	36.41704607847702	38.0	38.0	38.0	34.0	38.0
50-51	36.429636929223534	38.0	38.0	38.0	34.0	38.0
52-53	36.439383792816294	38.0	38.0	38.0	34.0	38.0
54-55	36.36516515109351	38.0	38.0	38.0	34.0	38.0
56-57	36.38153228056587	38.0	38.0	38.0	34.0	38.0
58-59	36.32445886862786	38.0	38.0	38.0	33.5	38.0
60-61	36.33841085400634	38.0	38.0	38.0	33.0	38.0
62-63	36.35856102159303	38.0	38.0	38.0	34.0	38.0
64-65	36.34880219150013	38.0	38.0	38.0	33.5	38.0
66-67	36.32806235016719	38.0	38.0	38.0	33.0	38.0
68-69	36.30549313304154	38.0	38.0	38.0	33.0	38.0
70-71	36.30435097753602	38.0	38.0	38.0	33.0	38.0
72-73	36.297433776019	38.0	38.0	38.0	33.0	38.0
74-75	36.26296684174562	38.0	37.5	38.0	33.0	38.0
76-77	36.25657809943792	38.0	37.0	38.0	33.0	38.0
78-79	36.241150417441986	38.0	37.0	38.0	33.0	38.0
80-81	36.250372226311285	38.0	37.0	38.0	33.0	38.0
82-83	36.219151470567525	38.0	37.0	38.0	33.0	38.0
84-85	36.194025425091695	38.0	37.0	38.0	33.0	38.0
86-87	36.19619490210181	38.0	37.0	38.0	33.0	38.0
88-89	36.20145492056281	38.0	37.0	38.0	33.0	38.0
90-91	36.146529473238076	38.0	37.0	38.0	33.0	38.0
92-93	36.168885831772755	38.0	37.0	38.0	33.0	38.0
94-95	36.12762319985523	38.0	37.0	38.0	33.0	38.0
96-97	36.16008307976437	38.0	37.0	38.0	33.0	38.0
98-99	36.206001829904395	38.0	37.0	38.0	33.0	38.0
100-101	35.68753727328804	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	19.0
19	73.0
20	191.0
21	629.0
22	2159.0
23	6460.0
24	14470.0
25	26628.0
26	44553.0
27	68117.0
28	96596.0
29	130390.0
30	166508.0
31	210960.0
32	269895.0
33	357925.0
34	508809.0
35	842758.0
36	1993485.0
37	4662401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.774974354000506	18.89269475592219	13.067410427239063	21.264920462838237
2	14.94198782391966	20.701378471143226	40.09642215176263	24.26021155317448
3	20.329200408464253	25.76664150455396	28.959443481279322	24.944714605702462
4	13.299197513651457	18.37932810140055	38.44273109528783	29.878743289660157
5	14.103579239635543	38.08678510219863	34.18997152389103	13.619664134274792
6	30.06014234141222	37.530546017845744	18.575988197841845	13.83332344290019
7	26.741042405380387	32.91334884700837	22.32773827867973	18.01787046893151
8	24.15606422868553	37.096994095305064	20.78243748342289	17.964504192586514
9	25.204109825921993	17.78148863993357	21.149872392142697	35.864529142001736
10-11	24.240994335227832	26.995836233995313	28.262923020738217	20.500246410038642
12-13	25.15013917594927	24.360489078754494	28.584576315357225	21.904795429939014
14-15	22.367012491510714	25.7654291288783	26.289834782973053	25.577723596637934
16-17	21.813541725823153	29.046601593997508	26.988434361449176	22.151422318730162
18-19	21.81938027184015	27.893813119308614	28.912357575104014	21.374449033747222
20-21	22.567839861072063	27.004282367052024	28.961022020905236	21.466855750970677
22-23	22.253349951963877	27.057877014972615	28.839435971989403	21.849337061074106
24-25	22.117276334124004	27.181028155680597	28.972269792475508	21.729425717719888
26-27	22.086792013842363	27.312317407121682	28.85193412079004	21.74895645824591
28-29	21.881956941980416	27.32301262880703	28.972110874490898	21.822919554721658
30-31	22.388617068909628	27.095138058179863	28.868512378929566	21.64773249398094
32-33	21.83227862679135	27.42814747739173	28.912739925876867	21.826833969940047
34-35	22.087071925249425	27.368938600250637	28.93821063930082	21.605778835199114
36-37	21.99108394508463	27.26770210999404	28.896288493918586	21.844925451002737
38-39	21.8267882774971	27.255362545294332	29.041487862950078	21.87636131425849
40-41	22.11972303815752	27.07457220371417	28.942689642549986	21.863015115578325
42-43	22.108854262671773	27.091622732362254	28.847193326004223	21.952329678961753
44-45	22.061991444021643	27.330146045039065	28.662808127034882	21.94505438390441
46-47	22.266723706957777	27.196094193244008	28.284651907519077	22.25253019227914
48-49	22.11262786481955	27.30177357944018	28.359545366300743	22.226053189439522
50-51	22.01975204084775	27.50342573737164	28.3659949100757	22.110827311704917
52-53	22.210337782018577	27.464804693387485	28.329312253734845	21.99554527085909
54-55	22.193601677176105	27.405909340355556	28.207759836520722	22.192729145947617
56-57	22.242002763750023	27.671141197442388	28.026249135933305	22.06060690287428
58-59	22.047093517721716	27.862869296061394	28.20014649589015	21.88989069032674
60-61	22.18271638609306	27.9587854883799	28.01980128780051	21.83869683772653
62-63	22.051643111463477	28.101720767850196	27.95481993803221	21.891816182654114
64-65	22.04416446538532	28.014277181752018	27.942646472648796	21.99891188021387
66-67	22.120080171123966	27.983014026368693	27.833047987581942	22.063857814925402
68-69	22.074870656476705	27.837195543243396	28.011234010046635	22.076699790233256
70-71	22.141835443972226	27.51134622927593	28.097627049126306	22.24919127762554
72-73	22.271673411069255	27.41475232033436	28.067808336385596	22.245765932210787
74-75	22.068026180817192	27.590683182136804	28.052316183715288	22.28897445333072
76-77	22.224844583105927	27.550330501494773	28.001853743589244	22.22297117181005
78-79	22.204445411844365	27.582923265813253	27.917115585001	22.295515737341383
80-81	22.19269648237337	27.594486447989336	27.931507487696106	22.281309581941187
82-83	22.278343300158276	27.565458568802647	27.96814934006633	22.188048790972747
84-85	22.391978071412726	27.53290315614803	27.880071000301427	22.195047772137812
86-87	22.210064699924978	27.555500825766384	27.97629564743301	22.258138826875633
88-89	22.264130750060055	27.604793525321536	27.906331631321745	22.224744093296668
90-91	22.2970377322037	27.60551288843854	27.93440288849548	22.163046490862286
92-93	22.33750919947238	27.590927832404372	27.86755060060869	22.204012367514558
94-95	22.21691680339247	27.690219979371232	27.868854009268762	22.22400920796754
96-97	22.290672737596985	27.645371597889756	27.91741808962923	22.14653757488403
98-99	22.479722602098228	28.274178305895408	27.935198238940213	21.31090085306615
100-101	23.10656886535042	30.802363339205314	25.32012669922754	20.770941096216728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1761.0
1	1639.5
2	2306.5
3	3384.0
4	4061.5
5	5736.5
6	7828.5
7	10358.5
8	13936.0
9	16100.0
10	15208.0
11	13612.0
12	13928.0
13	16732.5
14	22724.0
15	29771.5
16	35669.0
17	40221.5
18	41824.0
19	40006.0
20	36707.5
21	33558.5
22	32910.5
23	36628.5
24	44439.5
25	57082.5
26	73989.5
27	91835.5
28	109204.5
29	127698.0
30	145750.5
31	164454.5
32	185354.5
33	207795.0
34	230551.5
35	251019.5
36	273844.0
37	294820.0
38	309856.5
39	322794.5
40	336760.5
41	348440.5
42	356311.0
43	366915.0
44	389349.5
45	404483.5
46	400552.0
47	394709.5
48	388160.0
49	381017.5
50	373530.0
51	364081.5
52	349869.0
53	333197.0
54	316553.5
55	294930.5
56	270824.5
57	246185.5
58	220193.5
59	193698.5
60	163933.5
61	133169.5
62	104456.0
63	80143.5
64	60816.5
65	45240.0
66	33036.0
67	24488.0
68	18348.5
69	13410.5
70	9788.5
71	7036.5
72	4898.0
73	3227.0
74	2060.0
75	1336.0
76	864.5
77	508.0
78	297.0
79	181.5
80	117.0
81	73.5
82	53.0
83	46.5
84	35.0
85	23.0
86	17.0
87	11.0
88	5.0
89	2.0
90	0.5
91	1.0
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.04541091346551631
6	0.0
7	0.001478247534357557
8	0.0
9	0.0
10-11	0.0
12-13	5.317437173948046E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.319236929930064E-5
28-29	2.1286235692057562E-5
30-31	2.6635953636393663E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.502661224534716E-6
46-47	0.0
48-49	0.0
50-51	0.0
52-53	6.45937219208541E-4
54-55	1.7678706055584132E-4
56-57	0.002974649724945395
58-59	0.007351440743570178
60-61	0.003094401807550236
62-63	0.005406127872357623
64-65	0.006438898753195059
66-67	0.0046631133186626145
68-69	0.0036761830269847477
70-71	0.004986971309668565
72-73	0.00312571592684648
74-75	0.0029085108461518795
76-77	0.002228127243492297
78-79	6.276379623557813E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	5.848430515186945E-5
88-89	6.557884478930174E-6
90-91	1.390429488440466E-4
92-93	0.0
94-95	6.754666461324807E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	410.0
22-23	1017.0
24-25	1147.0
26-27	3101.0
28-29	7153.0
30-31	17860.0
32-33	22783.0
34-35	30790.0
36-37	46863.0
38-39	54907.0
40-41	53631.0
42-43	59743.0
44-45	70337.0
46-47	71800.0
48-49	64824.0
50-51	58457.0
52-53	55858.0
54-55	59892.0
56-57	62189.0
58-59	63442.0
60-61	63388.0
62-63	60741.0
64-65	70663.0
66-67	74251.0
68-69	71926.0
70-71	82273.0
72-73	61229.0
74-75	62381.0
76-77	66201.0
78-79	69265.0
80-81	69758.0
82-83	66806.0
84-85	66425.0
86-87	69210.0
88-89	72138.0
90-91	74566.0
92-93	73610.0
94-95	80673.0
96-97	135172.0
98-99	791507.0
100-101	6414540.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.9202489527784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.32962845118985	77.61144164338207
2	6.815538025152863	11.711854477365232
3	1.6147856094779132	4.162283447151189
4	0.5622077726947132	1.9322012717246724
5	0.2677830333631464	1.150399244594585
6	0.13823158801047752	0.7126135472998876
7	0.0791883917825195	0.4762720435286959
8	0.04979689312047975	0.3422849163189215
9	0.033371546654230665	0.2580562436823682
>10	0.10704116208784759	1.3832626973078144
>50	0.001905376260047267	0.10705375959638083
>100	4.441939862366693E-4	0.07243677895609303
>500	5.84673793670441E-5	0.04244865642201517
>1k	1.9488840330394815E-5	0.037391272670032547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.1904623043688275E-5	0.0	1.0634874347896092E-5	0.0
5	0.0	3.1904623043688275E-5	0.0	1.0634874347896092E-5	0.0
6	0.0	3.1904623043688275E-5	0.0	1.0634874347896092E-5	0.0
7	0.0	3.1904623043688275E-5	0.0	3.1904623043688275E-5	0.0
8	0.0	3.1904623043688275E-5	0.0	3.1904623043688275E-5	0.0
9	0.0	1.0634874347896092E-4	0.0	4.253949739158437E-5	0.0
10-11	0.0	1.0634874347896092E-4	0.0	6.380924608737655E-5	0.0
12-13	0.0	1.0634874347896092E-4	0.0	9.571386913106483E-5	0.0
14-15	0.0	1.8611030108818161E-4	0.0	9.571386913106483E-5	0.0
16-17	0.0	1.9142773826212966E-4	0.0	1.1166618065290897E-4	5.317437173948046E-6
18-19	0.0	2.2333236130581791E-4	0.0	1.2230105500080505E-4	2.6587185869740228E-5
20-21	0.0	2.552369843495062E-4	0.0	1.276184921747531E-4	3.1904623043688275E-5
22-23	0.0	2.765067330452984E-4	0.0	1.4888824087054529E-4	4.253949739158437E-5
24-25	0.0	3.0841135608898667E-4	0.0	1.9142773826212966E-4	7.444412043527264E-5
26-27	5.317437173948046E-6	3.0841135608898667E-4	0.0	3.0841135608898667E-4	8.507899478316874E-5
28-29	1.0634874347896092E-5	3.0841135608898667E-4	0.0	7.922981389182589E-4	9.039643195711678E-5
30-31	1.0634874347896092E-5	3.5095085348057104E-4	0.0	0.0022439584874060755	9.571386913106483E-5
32-33	1.0634874347896092E-5	3.5095085348057104E-4	0.0	0.004642122652856644	1.0634874347896092E-4
34-35	1.0634874347896092E-5	4.147600995679476E-4	0.0	0.00761988747026755	1.0634874347896092E-4
36-37	1.0634874347896092E-5	4.892042200032202E-4	0.0	0.011517568918771467	1.0634874347896092E-4
38-39	1.0634874347896092E-5	5.157914058729605E-4	0.0	0.01789849352750912	1.1698361782685702E-4
40-41	1.0634874347896092E-5	6.646796467435058E-4	0.0	0.024773939793423946	1.4357080369659724E-4
42-43	1.0634874347896092E-5	7.072191441350902E-4	0.0	0.0315536721902077	1.4888824087054529E-4
44-45	1.0634874347896092E-5	7.869807017443108E-4	0.0	0.038992766796561024	1.4888824087054529E-4
46-47	1.0634874347896092E-5	8.295201991358952E-4	0.0	0.04722947697900655	1.7547542674028552E-4
48-49	1.0634874347896092E-5	8.295201991358952E-4	0.0	0.05508333168492781	2.0206261261002575E-4
50-51	1.0634874347896092E-5	9.2523406826696E-4	0.0	0.06293186895367513	2.0206261261002575E-4
52-53	1.0634874347896092E-5	9.465038169627522E-4	0.0	0.07044540768046371	2.3928467282766206E-4
54-55	1.0634874347896092E-5	9.518212541367002E-4	0.0	0.07811315208529679	2.924590445671425E-4
56-57	1.0634874347896092E-5	9.890433143543366E-4	0.0	0.08405272940859676	3.0841135608898667E-4
58-59	1.0634874347896092E-5	0.001031582811745921	0.0	0.08935421427102297	3.0841135608898667E-4
60-61	1.0634874347896092E-5	0.0010688048719635574	0.0	0.09450681089257862	3.1904623043688276E-4
62-63	2.1269748695792184E-5	0.0010900746206593493	0.0	0.09959028083087296	3.4031597913267495E-4
64-65	2.1269748695792184E-5	0.0012442802987038429	0.0	0.1045089102167749	3.4031597913267495E-4
66-67	2.6587185869740228E-5	0.0012442802987038429	0.0	0.10927333392463234	3.4031597913267495E-4
68-69	3.1904623043688275E-5	0.0012602326102256868	0.0	0.11409093200422928	3.45633416306623E-4
70-71	3.1904623043688275E-5	0.001276184921747531	0.0	0.11849376998425826	3.934903508721554E-4
72-73	3.1904623043688275E-5	0.001276184921747531	0.0	0.12209367495102108	4.360298482637398E-4
74-75	3.1904623043688275E-5	0.0013187244191391154	0.0	0.12502358283386644	4.360298482637398E-4
76-77	3.1904623043688275E-5	0.0013612639165307	0.0	0.12770888860671023	4.4134728543768784E-4
78-79	3.1904623043688275E-5	0.001377216228052544	0.0	0.13001133890302974	4.466647226116359E-4
80-81	3.1904623043688275E-5	0.0014038034139222844	0.0	0.1310269694032538	4.838867828292722E-4
82-83	3.1904623043688275E-5	0.0014144382882701804	0.0	0.13148426900021332	5.423785917427007E-4
84-85	3.1904623043688275E-5	0.0014303905997920243	0.0	0.13195220347152076	5.423785917427007E-4
86-87	3.1904623043688275E-5	0.0014569777856617645	0.0	0.13203196502912998	5.423785917427007E-4
88-89	3.1904623043688275E-5	0.001488882408705453	0.0	0.13205323477782577	5.476960289166487E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13445	0.0	51.46482	1
GTATCAA	24190	0.0	38.157417	1
TCAACGC	28180	0.0	32.692944	4
ATCAACG	28405	0.0	32.311413	3
CAACGCA	29110	0.0	31.58868	5
TATCAAC	29235	0.0	31.185669	2
AACGCAG	30245	0.0	30.460611	6
CTTATAC	7400	0.0	27.28729	1
TGGTATC	5880	0.0	26.495974	2
ACGCAGA	35410	0.0	25.980692	7
GTGGTAT	6690	0.0	25.629744	1
CGCAGAG	36120	0.0	25.469725	8
TATACAC	11405	0.0	22.360243	3
GCAGAGT	41985	0.0	21.85999	9
GCGGTAT	630	0.0	21.413877	1
GAGTACT	24915	0.0	20.696756	12-13
CAGAGTA	40565	0.0	19.842632	10-11
GTACATG	29900	0.0	19.532394	1
TACATGG	29120	0.0	19.0543	2
TTATACA	11965	0.0	18.87682	2
>>END_MODULE