FastQCFastQC Report
Fri 27 May 2016
SRR2139677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10107030
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129750.048.971611
GTATCAA227400.036.9925961
TCAACGC258250.032.335654
ATCAACG262250.031.7587873
CAACGCA267550.031.1788675
TATCAAC272200.030.4689062
AACGCAG277900.030.0206666
CTTATAC79500.027.1017651
ACGCAGA326450.025.5693957
GTGGTAT66800.025.2253911
CGCAGAG332850.025.0642018
TGGTATC63900.024.104022
TATACAC122500.021.8512823
GCAGAGT399250.020.91779
GTACATG290050.020.0761871
GAGTACT243100.019.95547512-13
TTATACA123800.019.7786542
TACATGG285900.019.661572
ACATGGG286350.019.0943153
CAGAGTA387300.018.9753110-11
Produced by FastQC (version 0.11.5)