##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10107030
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.108228925807087	28.0	18.0	32.0	18.0	33.0
2	27.482552935926776	29.0	25.0	32.0	18.0	33.0
3	27.15938381502776	29.0	25.0	31.0	18.0	33.0
4	30.59480430947568	32.0	30.0	33.0	27.0	33.0
5	31.144551366721974	33.0	32.0	33.0	28.0	33.0
6	35.06301732556448	37.0	35.0	38.0	29.0	38.0
7	35.60577924474351	38.0	36.0	38.0	31.0	38.0
8	36.06867665377465	38.0	36.0	38.0	33.0	38.0
9	36.55372240905587	38.0	37.0	38.0	34.0	38.0
10-11	36.646154805120794	38.0	38.0	38.0	34.0	38.0
12-13	36.74504221319221	38.0	38.0	38.0	34.0	38.0
14-15	36.744753107490524	38.0	38.0	38.0	34.0	38.0
16-17	36.762542012836604	38.0	38.0	38.0	34.0	38.0
18-19	36.7733996535085	38.0	38.0	38.0	34.5	38.0
20-21	36.81415336197597	38.0	38.0	38.0	35.0	38.0
22-23	36.80015729174392	38.0	38.0	38.0	35.0	38.0
24-25	36.80491934274545	38.0	38.0	38.0	35.0	38.0
26-27	36.76280956313232	38.0	38.0	38.0	35.0	38.0
28-29	36.67932126062168	38.0	38.0	38.0	34.0	38.0
30-31	36.713894052705484	38.0	38.0	38.0	34.0	38.0
32-33	36.710452744339534	38.0	38.0	38.0	34.0	38.0
34-35	36.688291407760026	38.0	38.0	38.0	34.0	38.0
36-37	36.6570562107526	38.0	38.0	38.0	34.0	38.0
38-39	36.63893052425111	38.0	38.0	38.0	34.0	38.0
40-41	36.66016725366193	38.0	38.0	38.0	34.0	38.0
42-43	36.630150678583746	38.0	38.0	38.0	34.0	38.0
44-45	36.5726371945583	38.0	38.0	38.0	34.0	38.0
46-47	36.45687400619463	38.0	38.0	38.0	34.0	38.0
48-49	36.41078082901183	38.0	38.0	38.0	34.0	38.0
50-51	36.4233273120165	38.0	38.0	38.0	34.0	38.0
52-53	36.42902968801317	38.0	38.0	38.0	34.0	38.0
54-55	36.35170412659869	38.0	38.0	38.0	34.0	38.0
56-57	36.36638969797269	38.0	38.0	38.0	34.0	38.0
58-59	36.3082357102664	38.0	38.0	38.0	33.5	38.0
60-61	36.32131836641354	38.0	38.0	38.0	33.0	38.0
62-63	36.33781405749852	38.0	38.0	38.0	33.0	38.0
64-65	36.327131850196565	38.0	38.0	38.0	33.5	38.0
66-67	36.30635502355478	38.0	38.0	38.0	33.0	38.0
68-69	36.281187089877456	38.0	37.0	38.0	33.0	38.0
70-71	36.28079839516907	38.0	37.0	38.0	33.0	38.0
72-73	36.273311561867146	38.0	37.0	38.0	33.0	38.0
74-75	36.23756828758056	38.0	37.0	38.0	33.0	38.0
76-77	36.22981530272241	38.0	37.0	38.0	33.0	38.0
78-79	36.21564897637949	38.0	37.0	38.0	33.0	38.0
80-81	36.22018467827972	38.0	37.0	38.0	33.0	38.0
82-83	36.189332917203274	38.0	37.0	38.0	33.0	38.0
84-85	36.160257934204466	38.0	37.0	38.0	33.0	38.0
86-87	36.16012858639692	38.0	37.0	38.0	33.0	38.0
88-89	36.16532005647214	38.0	37.0	38.0	33.0	38.0
90-91	36.107385619407964	38.0	37.0	38.0	33.0	38.0
92-93	36.12778216612549	38.0	37.0	38.0	33.0	38.0
94-95	36.08677737273449	38.0	37.0	38.0	33.0	38.0
96-97	36.117997097283364	38.0	37.0	38.0	33.0	38.0
98-99	36.16261099575941	38.0	37.0	38.0	33.0	38.0
100-101	35.6226299724126	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	33.0
19	83.0
20	185.0
21	774.0
22	2577.0
23	7336.0
24	16117.0
25	30314.0
26	49927.0
27	74984.0
28	104927.0
29	139810.0
30	179835.0
31	227982.0
32	292283.0
33	387875.0
34	551309.0
35	916437.0
36	2166624.0
37	4957615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.68377357146461	19.122353451013797	13.032196401910353	21.16167657561123
2	14.85323581705011	20.933726327120823	40.13983336351035	24.073204492318713
3	20.349420156069588	26.01668343717195	28.950789697863765	24.6831067088947
4	13.254892881489418	18.590871898074905	38.77341810601136	29.380817114424318
5	14.071259440615302	38.15228577738188	34.364111907823144	13.41234287417967
6	29.71574240899651	37.86041992553697	18.791840926562998	13.631996738903515
7	26.497909240600485	32.899802450891244	22.706472261299115	17.895816047209152
8	23.774234369542786	37.43008579177068	21.02638460556662	17.769295233119916
9	24.98862672812884	17.93168715240778	21.53920587947201	35.540480239991375
10-11	24.07053308439769	27.185622284686993	28.47609040440169	20.267754226513624
12-13	24.896200961113205	24.513566299892254	28.86910398010098	21.721128758893563
14-15	22.120810960291994	25.961617804636973	26.463491253117876	25.454079981953154
16-17	21.66790837664477	29.212157280625465	27.170504094674698	21.949430248055066
18-19	21.601533783910803	28.227515897350656	28.99974077449063	21.171209544247915
20-21	22.280517078652643	27.315935112171864	29.081884704611927	21.321663104563566
22-23	22.056442741507414	27.37114128614733	28.900578701636565	21.67183727070869
24-25	21.87424174113849	27.515657983641994	29.05744935276112	21.552650922458398
26-27	21.84782505421628	27.65838418460218	28.990448908429713	21.503341852751827
28-29	21.71753411576477	27.471397656665943	29.15040341413827	21.660664813431016
30-31	21.983119285546394	27.55400179715317	28.954217582373154	21.50866133492728
32-33	21.964445791758745	27.495475396310415	28.982308856649297	21.55776995528154
34-35	21.926073099809788	27.50371551512268	28.989066022038866	21.58114536302867
36-37	21.828838000394523	27.556685417881255	28.92111161140299	21.693364970321234
38-39	21.716356202430344	27.496225662208083	29.086433526156753	21.700984609204816
40-41	21.946521619482137	27.343970662141192	29.022369190128416	21.687138528248255
42-43	21.905305584076206	27.38504601603786	28.93066214568613	21.778986254199793
44-45	21.871090744243048	27.557975407925827	28.777952412895786	21.79298143493533
46-47	22.086928861292815	27.48368633356457	28.378715093768008	22.050669711374603
48-49	21.976873574739205	27.544230529831022	28.4350913580252	22.043804537404572
50-51	21.87874288552735	27.741430287216478	28.430833659562836	21.948993167693338
52-53	21.997533944868145	27.72764985866416	28.438052812240066	21.836763384227623
54-55	21.992299258564977	27.679465908006094	28.298949294028574	22.029285539400355
56-57	22.040396731459815	27.910769716698713	28.169375313710717	21.879458238130752
58-59	21.907633644347648	28.108988551752077	28.262761843534452	21.720615960365823
60-61	22.032853158964194	28.198555680228193	28.0854976644079	21.68309349639971
62-63	21.8940732209798	28.311552588898714	28.078728626022293	21.71564556409919
64-65	21.898650138065854	28.27624885334516	28.023296103375767	21.801804905213217
66-67	21.924812978524198	28.173805238588745	28.022208302369993	21.879173480517064
68-69	21.931151760338047	28.054680878887527	28.094182781752043	21.91998457902238
70-71	21.96600611150736	27.773564069455155	28.23949175048964	22.020938068547842
72-73	22.085477545824382	27.710931506200247	28.165467314909076	22.03812363306629
74-75	21.958881968022073	27.856119955243447	28.12561453350001	22.059383543234475
76-77	22.08516194234257	27.755745658021425	28.10955759048085	22.049534809155162
78-79	21.99288177016187	27.77939648732952	28.08253594438737	22.145185798121236
80-81	22.037942508784948	27.800699418777864	28.09972997503761	22.061628097399584
82-83	22.07282909899754	27.80740368552173	28.14332545649338	21.97644175898735
84-85	22.171646717661044	27.78605245819097	28.055863755339296	21.986437068808694
86-87	22.043772137362865	27.812970344029996	28.140241869986326	22.00301564862081
88-89	22.123768061795136	27.790103204628892	28.03111918040001	22.055009553175964
90-91	22.143894628774287	27.84850115659352	28.01243296621289	21.9951712484193
92-93	22.124068995673213	27.811806597872625	28.05966110463924	22.004463301814923
94-95	22.019004460648752	27.932420867764808	28.035183829441213	22.01339084214523
96-97	22.120477330024578	27.887794357474732	28.053519648002634	21.93820866449806
98-99	22.326819518339118	28.438212658400197	28.109800253040536	21.125167570220153
100-101	22.933983206384568	30.991358963903032	25.53097105161571	20.543686778096696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1427.0
1	1361.5
2	2027.0
3	3072.5
4	3876.5
5	5531.5
6	7464.0
7	9683.5
8	12725.0
9	14767.0
10	14157.0
11	12824.5
12	13350.5
13	16087.5
14	21854.0
15	28900.0
16	34855.0
17	39373.0
18	40684.5
19	38881.5
20	36259.0
21	33962.0
22	34188.0
23	38637.0
24	47579.5
25	61461.5
26	78778.0
27	97467.5
28	118355.0
29	141888.0
30	164555.0
31	186630.5
32	210553.5
33	235673.5
34	259742.0
35	280460.5
36	303252.5
37	326196.0
38	345043.0
39	359727.5
40	372578.0
41	386790.0
42	396386.0
43	406012.0
44	427390.0
45	440641.0
46	434362.0
47	424943.0
48	415013.5
49	403930.5
50	393498.0
51	381054.5
52	361777.5
53	340707.5
54	319935.5
55	296183.5
56	270283.5
57	242595.0
58	215184.5
59	185884.0
60	154780.5
61	126201.5
62	99308.0
63	74984.0
64	55218.5
65	40194.0
66	29571.0
67	22219.0
68	16551.0
69	11747.5
70	8113.0
71	5650.5
72	3733.5
73	2225.5
74	1353.0
75	829.5
76	528.0
77	335.5
78	222.5
79	138.0
80	86.0
81	59.0
82	45.0
83	33.5
84	22.5
85	16.0
86	13.0
87	11.0
88	8.5
89	5.5
90	3.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.044889547176569185
6	0.0
7	0.0017413622003694458
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.948544539028725E-5
28-29	2.4751605265359484E-5
30-31	2.972793293061233E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.096190078105228E-6
46-47	0.0
48-49	0.0
50-51	0.0
52-53	6.119076283072234E-4
54-55	1.7357012927503228E-4
56-57	0.0028260951250917554
58-59	0.0069403039054150035
60-61	0.0030350943780292627
62-63	0.005521916069250765
64-65	0.00609049001224835
66-67	0.004620676157119414
68-69	0.0035437846460838283
70-71	0.00463823596916915
72-73	0.002941638454445192
74-75	0.0026961731637280264
76-77	0.0021177713121232843
78-79	6.310381748264875E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	5.9204626107392926E-5
88-89	0.0
90-91	1.8671700075156603E-4
92-93	0.0
94-95	7.975626485460433E-5
96-97	6.202989865927336E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	100.0
20-21	393.0
22-23	922.0
24-25	1170.0
26-27	2780.0
28-29	6173.0
30-31	16340.0
32-33	20821.0
34-35	28246.0
36-37	44285.0
38-39	51235.0
40-41	50320.0
42-43	56529.0
44-45	67843.0
46-47	68300.0
48-49	62413.0
50-51	55794.0
52-53	53131.0
54-55	57608.0
56-57	60250.0
58-59	61677.0
60-61	62699.0
62-63	59334.0
64-65	68835.0
66-67	74181.0
68-69	72284.0
70-71	81418.0
72-73	61074.0
74-75	61756.0
76-77	66193.0
78-79	68746.0
80-81	69737.0
82-83	65743.0
84-85	66447.0
86-87	69153.0
88-89	73106.0
90-91	75474.0
92-93	74659.0
94-95	83344.0
96-97	142948.0
98-99	865777.0
100-101	7077792.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.73072131680411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54791919225651	75.8745978557878
2	7.2410799141157485	12.270838484712986
3	1.7743493766287717	4.5102570764933265
4	0.6650296717004937	2.25393775121041
5	0.29873451604783513	1.265599551347973
6	0.1634615227110422	0.8310127636109856
7	0.09024423423405464	0.5352521342933853
8	0.056952801528449476	0.38605215636146456
9	0.03893887470401448	0.29693870468422234
>10	0.12058432408372252	1.5572743429023932
>50	0.002185504446364308	0.12369653674893788
>100	4.620271750115364E-4	0.05405339109672827
>500	3.869447263626067E-5	0.022900724107059105
>1k	1.934589540478013E-5	0.017588526642346187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.894103411190032E-6
2	0.0	4.9470517055950165E-5	0.0	1.9788206822380065E-5	9.894103411190032E-6
3	0.0	4.9470517055950165E-5	0.0	1.9788206822380065E-5	1.9788206822380065E-5
4	0.0	1.0883513752309037E-4	0.0	1.9788206822380065E-5	2.96823102335701E-5
5	0.0	1.187292409342804E-4	0.0	1.9788206822380065E-5	2.96823102335701E-5
6	0.0	1.187292409342804E-4	0.0	1.9788206822380065E-5	5.93646204671402E-5
7	0.0	1.187292409342804E-4	0.0	5.93646204671402E-5	6.925872387833024E-5
8	0.0	1.484115511678505E-4	0.0	6.925872387833024E-5	6.925872387833024E-5
9	0.0	2.8692899892451097E-4	0.0	1.0883513752309037E-4	1.3851744775666047E-4
10-11	0.0	2.96823102335701E-4	0.0	1.5830565457904052E-4	1.3851744775666047E-4
12-13	0.0	2.96823102335701E-4	0.0	1.6819975799023054E-4	1.5335860287344553E-4
14-15	0.0	4.303934983867665E-4	0.0	1.9293501651820565E-4	1.9293501651820565E-4
16-17	1.9788206822380065E-5	4.3534055009236146E-4	0.0	1.9788206822380066E-4	1.9788206822380066E-4
18-19	3.957641364476013E-5	4.5512875691474156E-4	0.0	2.1767027504618073E-4	2.2261732675177574E-4
20-21	3.957641364476013E-5	4.8481106714831163E-4	0.0	2.2261732675177574E-4	2.5229963698534586E-4
22-23	8.90469307007103E-5	4.996522222650967E-4	0.0	2.374584818685608E-4	2.7703489551332095E-4
24-25	1.2862334434547042E-4	5.639638944378319E-4	0.0	2.720878438077259E-4	3.06717205746891E-4
26-27	1.2862334434547042E-4	5.98593256376997E-4	0.0	3.611347745084362E-4	3.2155836086367607E-4
28-29	1.2862334434547042E-4	6.035403080825921E-4	0.0	6.826931353721122E-4	3.265054125692711E-4
30-31	1.4346449946225548E-4	7.321636524280624E-4	0.0	0.001845250286186941	3.265054125692711E-4
32-33	1.5830565457904052E-4	7.321636524280624E-4	0.0	0.004031847140059938	3.660818262140312E-4
34-35	1.5830565457904052E-4	7.964753246007977E-4	0.0	0.006678519802553273	4.0071118815319636E-4
36-37	2.0282911992939567E-4	8.607869967735328E-4	0.0	0.010369020374927155	4.204993949755764E-4
38-39	2.1767027504618073E-4	8.805752035959129E-4	0.0	0.016335164731874744	4.3534055009236146E-4
40-41	2.2261732675177574E-4	0.0010388808581749535	0.0	0.022697073225269936	4.452346535035515E-4
42-43	2.2756437845737075E-4	0.0010932984269364987	0.0	0.028603852961750387	4.501817052091465E-4
44-45	2.2756437845737075E-4	0.0011675042025204238	0.0	0.03504986133414069	4.551287569147415E-4
46-47	2.2756437845737075E-4	0.0012070806161651841	0.0	0.04259411518517309	4.551287569147415E-4
48-49	2.4240553357415581E-4	0.0012070806161651841	0.0	0.04960903450370682	4.6502286032593153E-4
50-51	2.473525852797508E-4	0.0013851744775666046	0.0	0.05674763011488043	4.749169637371216E-4
52-53	2.473525852797508E-4	0.0014148567878001746	0.0	0.0634310969691393	4.947051705595016E-4
54-55	2.5724668869094085E-4	0.0014148567878001746	0.0	0.07075273349341993	5.194404290874767E-4
56-57	2.6714079210213087E-4	0.0014247508912113647	0.0	0.07602629061158421	5.293345324986667E-4
58-59	2.6714079210213087E-4	0.0014346449946225548	0.0	0.08049842535344211	5.441756876154518E-4
60-61	2.6714079210213087E-4	0.0014643273048561248	0.0	0.08463416057931955	5.441756876154518E-4
62-63	2.720878438077259E-4	0.00149895666679529	0.0	0.08872042528814103	5.837521012602119E-4
64-65	2.8692899892451097E-4	0.0017017857867246858	0.0	0.09327171285728844	5.837521012602119E-4
66-67	2.96823102335701E-4	0.0017017857867246858	0.0	0.09739755397975469	6.084873597881871E-4
68-69	2.96823102335701E-4	0.0017265210452526607	0.0	0.10168664780850556	6.332226183161621E-4
70-71	2.96823102335701E-4	0.0017562033554862307	0.0	0.10535736017405707	6.530108251385422E-4
72-73	2.96823102335701E-4	0.001775991562308611	0.0	0.10865704366168895	7.074283939000874E-4
74-75	2.96823102335701E-4	0.0018106209242477762	0.0	0.11124929875542072	7.123754456056824E-4
76-77	2.96823102335701E-4	0.0019046149066540815	0.0	0.11358925421216717	7.272166007224674E-4
78-79	2.96823102335701E-4	0.0019392442685932465	0.0	0.11590447441038565	7.420577558392525E-4
80-81	2.96823102335701E-4	0.0019441913202988415	0.0	0.11669600268328084	7.618459626616325E-4
82-83	2.96823102335701E-4	0.0019491383720044366	0.0	0.11708187271631726	7.964753246007977E-4
84-85	2.96823102335701E-4	0.0019639795271212215	0.0	0.1176260484039327	8.212105831287728E-4
86-87	2.96823102335701E-4	0.0019887147856491967	0.0	0.1178239304721565	8.212105831287728E-4
88-89	2.96823102335701E-4	0.002147020440228237	0.0	0.11790803035115162	8.212105831287728E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12975	0.0	48.97161	1
GTATCAA	22740	0.0	36.992596	1
TCAACGC	25825	0.0	32.33565	4
ATCAACG	26225	0.0	31.758787	3
CAACGCA	26755	0.0	31.178867	5
TATCAAC	27220	0.0	30.468906	2
AACGCAG	27790	0.0	30.020666	6
CTTATAC	7950	0.0	27.101765	1
ACGCAGA	32645	0.0	25.569395	7
GTGGTAT	6680	0.0	25.225391	1
CGCAGAG	33285	0.0	25.064201	8
TGGTATC	6390	0.0	24.10402	2
TATACAC	12250	0.0	21.851282	3
GCAGAGT	39925	0.0	20.9177	9
GTACATG	29005	0.0	20.076187	1
GAGTACT	24310	0.0	19.955475	12-13
TTATACA	12380	0.0	19.778654	2
TACATGG	28590	0.0	19.66157	2
ACATGGG	28635	0.0	19.094315	3
CAGAGTA	38730	0.0	18.97531	10-11
>>END_MODULE