FastQCFastQC Report
Fri 27 May 2016
SRR2139676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15750807
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157300.049.447631
GTATCAA294700.035.9370841
ATCAACG350750.029.3439313
TCAACGC364750.028.2293054
CAACGCA375200.027.4203875
AACGCAG382900.026.9801796
TATCAAC391150.026.5422442
ACGCAGA394750.026.1060897
CGCAGAG465400.022.1156278
GTACATG443100.020.0121631
ACATGGG429150.019.392773
TACATGG446700.019.345732
AGAGTAC491300.018.52917110-11
GCAGAGT554050.018.338989
GAGTACT292150.017.34593412-13
CAGAGTA539600.016.36595210-11
CATGGGG315800.016.2889964
AGTACTT332850.016.03666112-13
GTACTTT337400.015.13346514-15
GTGGTAT85100.014.0499521
Produced by FastQC (version 0.11.5)