##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15750807
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797056112743938	31.0	31.0	34.0	30.0	34.0
2	31.992871603340706	31.0	31.0	34.0	30.0	34.0
3	32.72316072439971	34.0	31.0	34.0	30.0	37.0
4	35.60115789622716	37.0	35.0	37.0	33.0	37.0
5	35.4654506273869	37.0	35.0	37.0	33.0	37.0
6	35.44589321677296	37.0	35.0	37.0	33.0	37.0
7	35.39119957472655	37.0	35.0	37.0	32.0	37.0
8	35.63442120775145	37.0	35.0	37.0	32.0	39.0
9	36.814481188170234	39.0	37.0	39.0	32.0	39.0
10-11	36.749895132357345	39.0	35.0	39.0	32.0	39.0
12-13	36.83011279993463	39.0	35.5	39.0	32.0	39.5
14-15	37.7485729461354	39.0	37.0	41.0	32.0	41.0
16-17	37.7802373554574	39.0	37.0	41.0	32.0	41.0
18-19	37.818795602028516	40.0	37.0	41.0	32.0	41.0
20-21	37.787217579416364	40.0	37.0	41.0	32.0	41.0
22-23	37.716002456078485	39.0	37.0	41.0	32.0	41.0
24-25	37.60989433479469	39.0	36.5	41.0	32.0	41.0
26-27	37.44461094037633	39.0	36.0	41.0	32.0	41.0
28-29	37.30578176586525	39.0	36.0	40.5	31.0	41.0
30-31	37.20120818701127	39.0	36.0	40.0	31.0	41.0
32-33	37.39620041227005	39.0	36.0	41.0	32.0	41.0
34-35	37.384711527602676	39.0	36.0	41.0	31.0	41.0
36-37	37.261543388882146	39.0	36.0	41.0	31.0	41.0
38-39	37.16381571446004	39.0	36.0	41.0	31.0	41.0
40-41	37.138126690019305	39.0	36.0	40.0	31.0	41.0
42-43	37.06649236707839	39.0	36.0	40.0	31.0	41.0
44-45	37.05245132860193	39.0	35.5	40.0	31.0	41.0
46-47	37.04498217766755	39.0	36.0	40.0	31.0	41.0
48-49	36.965951709811144	39.0	35.0	40.0	30.5	41.0
50-51	36.87432753724849	39.0	35.0	40.0	30.0	41.0
52-53	36.749361861091074	39.0	35.0	40.0	30.0	41.0
54-55	36.63241006759734	38.5	35.0	40.0	30.0	41.0
56-57	36.45709792216243	38.0	35.0	40.0	30.0	41.0
58-59	36.314400468129605	38.0	35.0	40.0	29.5	41.0
60-61	36.1382429124075	38.0	34.0	40.0	29.0	41.0
62-63	35.90757561791848	38.0	34.0	40.0	29.0	41.0
64-65	35.69586271453086	37.0	34.0	40.0	29.0	41.0
66-67	35.43614689356308	37.0	34.0	39.5	28.5	41.0
68-69	35.156601914278774	36.0	34.0	39.0	28.0	40.5
70-71	34.870749082851546	36.0	33.0	39.0	28.0	40.0
72-73	34.55653039706225	35.5	33.0	38.5	28.0	40.0
74-75	34.11723608997739	35.0	32.5	37.5	28.0	39.5
76-77	33.28402459525252	34.5	31.5	36.5	26.5	39.0
78-79	33.58539182937379	35.0	32.0	36.5	27.5	39.0
80-81	33.49286757504377	35.0	32.0	36.0	28.0	38.5
82-83	33.2958651526498	35.0	32.0	36.0	27.5	37.5
84-85	33.07440760428838	35.0	32.0	35.5	27.0	37.0
86-87	32.90276860606623	34.0	32.0	35.0	27.0	36.5
88-89	32.72646730399644	34.0	32.0	35.0	27.0	36.0
90-91	32.63905109831475	34.0	32.0	35.0	27.0	36.0
92-93	32.60448335704503	34.0	32.0	35.0	27.0	36.0
94-95	32.586244200753725	34.0	32.0	35.0	27.0	35.0
96-97	32.52616910226941	34.0	32.0	35.0	27.0	35.0
98-99	32.65570964074304	34.0	32.0	35.0	28.5	35.0
100-101	32.11020792970416	34.0	30.5	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	21.0
20	135.0
21	865.0
22	4213.0
23	14744.0
24	37571.0
25	78909.0
26	139308.0
27	214835.0
28	301726.0
29	393878.0
30	490465.0
31	598911.0
32	728962.0
33	897010.0
34	1138562.0
35	1478680.0
36	2054921.0
37	2943341.0
38	3002375.0
39	1181002.0
40	50370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07484747925228	0.010577345281881808	28.0157497052184	30.898825470247438
2	13.939059865753881	26.669089450895257	37.05025639228381	22.34159429106705
3	14.97364547733967	26.402075779355304	34.616791380911465	24.007487362393558
4	12.584910728701077	25.444626424538118	37.76575384359671	24.20470900316409
5	16.622430837988176	37.592588113104306	32.761724526241736	13.023256522665791
6	26.5831903089156	38.68884940308138	21.18038142426607	13.54757886373695
7	24.87463023323186	33.360614475182125	23.768610713089178	17.996144578496835
8	23.796647371782285	32.49473503167171	22.72578795486479	20.982829641681217
9	23.119167560007252	20.76665922789343	24.577556512190966	31.536616699908354
10-11	24.13743757302009	26.44299953742038	29.557713950057906	19.86184893950162
12-13	24.438289794614114	24.507639124195997	29.168592772394202	21.885478308795683
14-15	21.889157806327002	27.005451212753734	26.962440718116852	24.142950262802408
16-17	21.224647662944427	29.05803813097266	27.911283061552812	21.806031144530102
18-19	21.404843778351804	28.385937772407456	29.264031797318758	20.945186651921983
20-21	22.12768298545484	27.790023444738832	28.957301661068207	21.12499190873812
22-23	21.988684151620937	27.486833896109463	28.79953263387166	21.724949318397936
24-25	21.710445625348743	27.784478014082143	28.91410757901951	21.59096878154961
26-27	21.696872626879934	27.981182093142493	28.72559079907256	21.596354480905013
28-29	21.804771064322026	27.764510588192064	28.74810149218852	21.68261685529739
30-31	21.941330734770993	27.702563381959642	28.764539271944333	21.591566611325028
32-33	21.736039772536184	28.048197310849414	28.68313484628404	21.532628070330357
34-35	21.917397540276344	27.79972529123092	28.624289419557886	21.65858774893485
36-37	21.862695118671173	27.869670980236855	28.684057306262247	21.58357659482973
38-39	21.698746495184952	28.082960944635072	28.687391476549095	21.530901083630873
40-41	21.978143160476083	27.71079085365391	28.50912446602885	21.801941519841154
42-43	21.761156208097976	27.84087025444441	28.562762608316493	21.83521092914112
44-45	21.869462659203663	28.09889879985356	28.221127927880634	21.81051061306214
46-47	22.00895165534163	27.837367600769653	28.072365804957926	22.08131493893079
48-49	21.820794238593894	28.021725873295434	28.148051774913885	22.009428113196787
50-51	21.891420436792263	28.246873970863106	27.915526048730342	21.946179543614285
52-53	22.03838511689924	27.9529941732294	27.836708902877106	22.171911806994252
54-55	21.949867161105907	27.87074170507934	28.050863748085213	22.128527385729537
56-57	21.914496415158286	28.19395763826602	27.862092203355342	22.029453743220348
58-59	22.02320233680101	27.934488322190298	27.90917441653414	22.133134924474554
60-61	21.979200883673368	28.012081243549407	27.971737372993733	22.036980499783493
62-63	21.933963468156943	28.223000653721748	27.873741656539412	21.96929422158189
64-65	22.011984495098826	27.95088778755732	27.830652093822916	22.206475623520937
66-67	21.918070878601288	28.129562667733044	27.770097279563917	22.18226917410175
68-69	21.946639586525325	28.230627161940557	27.7218806735782	22.10085257795592
70-71	22.027554779507323	27.79669791609344	27.831401296250707	22.34434600814853
72-73	21.937981151584417	27.95093095045748	27.754563378537668	22.356524519420436
74-75	21.87005378307094	28.13995731271781	27.751252975436092	22.238735928775156
76-77	22.15094634927359	27.893892856206687	27.569669142724525	22.385491651795203
78-79	21.956256287730014	27.948671006592807	27.730775795215617	22.36429691046156
80-81	22.022385189774706	28.073242869270643	27.579012050494317	22.325359890460334
82-83	22.126703792969803	27.84863767641103	27.689242226729228	22.33541630388994
84-85	22.031156434761385	27.940896863470172	27.800117825845906	22.227828875922537
86-87	21.999990417826577	28.053745509840628	27.723283375129764	22.22298069720303
88-89	22.101120089236428	27.884350206065573	27.701657783982515	22.312871920715484
90-91	22.065278211932302	27.897883244731958	27.830413974162198	22.206424569173542
92-93	22.04871756125854	27.96420136647218	27.80901026601686	22.178070806252414
94-95	21.993892633760098	27.750789413516053	27.93442434275778	22.320893609966063
96-97	21.808671007515162	27.9798238128339	28.075715316420137	22.135789863230805
98-99	22.160868359394048	28.982264275701386	27.97880133508373	20.878066029820836
100-101	22.641117570450135	30.99570976102418	24.90713852514266	21.456034143383025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2897.0
1	2640.5
2	3702.0
3	5259.5
4	6661.0
5	9773.0
6	12335.0
7	14733.0
8	16823.5
9	17199.0
10	16245.5
11	14914.5
12	16275.5
13	20999.0
14	33355.0
15	52694.5
16	69544.0
17	78483.0
18	79590.5
19	76213.5
20	71320.0
21	67816.0
22	68318.5
23	75584.0
24	91076.5
25	114773.0
26	146127.0
27	180993.0
28	217619.5
29	256462.5
30	293977.0
31	329879.0
32	368497.5
33	408126.0
34	446379.5
35	481546.5
36	520184.0
37	554430.5
38	576010.5
39	594528.0
40	612976.0
41	629101.5
42	638664.0
43	645644.5
44	651430.5
45	655315.5
46	654056.5
47	646782.5
48	638108.5
49	625652.0
50	609254.5
51	587263.0
52	559785.0
53	528589.5
54	493363.0
55	455577.0
56	415685.0
57	375211.5
58	332987.5
59	286490.5
60	237783.0
61	192501.5
62	152277.0
63	116837.5
64	86997.0
65	64545.5
66	48147.5
67	36405.5
68	27673.0
69	19937.0
70	14011.0
71	9835.0
72	6673.5
73	4426.0
74	2960.5
75	1973.0
76	1372.0
77	929.5
78	614.0
79	429.5
80	310.5
81	221.0
82	164.0
83	118.0
84	84.5
85	57.0
86	42.0
87	32.5
88	24.0
89	20.0
90	14.5
91	10.0
92	9.5
93	5.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010348676102754607
2	0.0021141773878633648
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.2697762089269458E-5
10-11	3.1744405223173647E-5
12-13	7.237724390883591E-4
14-15	0.0
16-17	9.84076561918383E-5
18-19	2.8569964700856276E-4
20-21	6.984115233075958E-5
22-23	0.0014223984190041574
24-25	8.511076689945732E-4
26-27	0.004276238663678595
28-29	2.606458244093925E-4
30-31	9.54605000201899E-6
32-33	3.82538433875538E-5
34-35	0.001169639756707261
36-37	0.02147270760802666
38-39	0.010731666513334112
40-41	0.011250133005500113
42-43	0.001690822801378719
44-45	0.0027067741974102057
46-47	1.2938586138322046E-4
48-49	5.320577332834738E-4
50-51	3.710879445030557E-5
52-53	2.9228420342885397E-4
54-55	0.005063044315775946
56-57	0.010030842684641349
58-59	0.009734099008211202
60-61	0.006276333953649607
62-63	0.006288662958647893
64-65	6.404991169878793E-4
66-67	7.590133650326228E-5
68-69	2.5576710986965815E-5
70-71	9.239443049329608E-5
72-73	3.738695400185843E-5
74-75	1.7019212080826733E-4
76-77	7.653368237794744E-5
78-79	3.952064250335838E-4
80-81	0.0020657653279590967
82-83	0.0031496622335668465
84-85	0.009098098327919682
86-87	0.018047263933660747
88-89	0.03254708984231815
90-91	5.247206254094355E-4
92-93	5.307346951755482E-4
94-95	0.0014915812813609927
96-97	0.008011916930699192
98-99	9.685318669502592E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	504.0
20-21	1574.0
22-23	3120.0
24-25	5706.0
26-27	7270.0
28-29	13309.0
30-31	26567.0
32-33	35254.0
34-35	52505.0
36-37	77450.0
38-39	86550.0
40-41	96954.0
42-43	110232.0
44-45	129391.0
46-47	131120.0
48-49	122984.0
50-51	113482.0
52-53	103182.0
54-55	113468.0
56-57	121285.0
58-59	129520.0
60-61	133000.0
62-63	129349.0
64-65	136795.0
66-67	148055.0
68-69	154073.0
70-71	156090.0
72-73	153404.0
74-75	154430.0
76-77	156834.0
78-79	171815.0
80-81	175879.0
82-83	169743.0
84-85	172838.0
86-87	183552.0
88-89	203251.0
90-91	223614.0
92-93	244994.0
94-95	309616.0
96-97	471180.0
98-99	1591111.0
100-101	9029757.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.04643973214694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.12775480135254	74.90870472373446
2	7.525912514306979	12.650523051262239
3	1.84178131723872	4.643854874372948
4	0.6989286805411616	2.349698689046869
5	0.3126007118354879	1.3136488443753784
6	0.16727699761177503	0.8435421659011523
7	0.09629827533813684	0.5665469036161487
8	0.06012824041178596	0.40428516271753684
9	0.03793852326634776	0.28697380283085766
>10	0.12684338600059608	1.65787236117249
>50	0.0035930359617348732	0.20651663328523745
>100	8.76794953158441E-4	0.12272540008927574
>500	5.3388345567308395E-5	0.03151478370081775
>1k	1.3332836063731622E-5	0.013592603894617753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.348881044634729E-6
2	0.0	4.44421673124431E-5	0.0	6.348881044634729E-6	6.348881044634729E-6
3	0.0	4.44421673124431E-5	0.0	6.348881044634729E-6	6.348881044634729E-6
4	0.0	4.44421673124431E-5	0.0	6.348881044634729E-6	6.348881044634729E-6
5	6.348881044634729E-6	4.44421673124431E-5	0.0	1.2697762089269458E-5	6.348881044634729E-6
6	3.1744405223173647E-5	4.44421673124431E-5	0.0	1.9046643133904185E-5	6.348881044634729E-6
7	4.44421673124431E-5	5.079104835707783E-5	0.0	1.9046643133904185E-5	1.2697762089269458E-5
8	5.079104835707783E-5	5.079104835707783E-5	0.0	1.9046643133904185E-5	1.2697762089269458E-5
9	5.713992940171256E-5	1.0158209671415566E-4	0.0	2.5395524178538916E-5	1.9046643133904185E-5
10-11	6.031436992402993E-5	1.0158209671415566E-4	0.0	3.491884574549101E-5	1.9046643133904185E-5
12-13	6.348881044634729E-5	1.0158209671415566E-4	0.0	3.809328626780837E-5	1.9046643133904185E-5
14-15	6.348881044634729E-5	1.5554758559355086E-4	0.0	5.713992940171256E-5	4.444216731244311E-5
16-17	1.0793097775879039E-4	1.777686692497724E-4	0.0	8.253545358025148E-5	5.713992940171256E-5
18-19	1.0793097775879039E-4	1.777686692497724E-4	0.0	2.5078080126307177E-4	6.983769149098202E-5
20-21	1.1110541828110776E-4	1.872919908167245E-4	0.0	5.015616025261435E-4	7.618657253561674E-5
22-23	1.1427985880342512E-4	2.222108365622155E-4	0.0	6.983769149098201E-4	9.523321566952094E-5
24-25	1.2697762089269459E-4	2.3173415812916762E-4	0.0	0.0011078797422887602	1.2062873984805985E-4
26-27	1.333265019373293E-4	2.3490859865148497E-4	0.0	0.002558599060987796	1.238031803703772E-4
28-29	1.3967538298196403E-4	2.3808303917380232E-4	0.0	0.010205826279250327	1.2697762089269459E-4
30-31	1.3967538298196403E-4	2.7935076596392807E-4	0.0	0.017230863155138654	1.3650094245964669E-4
32-33	1.5237314507123348E-4	2.8252520648624547E-4	0.0	0.026166913225462034	1.4602426402659876E-4
34-35	1.5872202611586823E-4	3.3649069536564066E-4	0.0	0.038232961650790334	1.5237314507123348E-4
36-37	1.6507090716050296E-4	3.936306247673532E-4	0.0	0.05593046756270964	1.5237314507123348E-4
38-39	1.6507090716050296E-4	4.0632838685662265E-4	0.0	0.08828436536616822	1.6189646663818558E-4
40-41	1.6507090716050296E-4	4.2537502999052685E-4	0.0	0.1380786393992384	1.7459422872745503E-4
42-43	1.968153123836766E-4	5.079104835707783E-4	0.0	0.1683691508631907	1.8411755029440713E-4
44-45	1.968153123836766E-4	5.904459371510298E-4	0.0	0.20780839991246164	1.968153123836766E-4
46-47	1.968153123836766E-4	5.904459371510298E-4	0.0	0.24726987004538878	2.0633863395062868E-4
48-49	2.0633863395062868E-4	5.936203776733472E-4	0.0	0.2814776411138807	2.1903639603989812E-4
50-51	2.0951307447294605E-4	6.571091881196944E-4	0.0	0.3157171565875958	2.222108365622155E-4
52-53	2.1586195551758077E-4	6.634580691643292E-4	0.0	0.36360676630727556	2.666530038746586E-4
54-55	2.1586195551758077E-4	6.793302717759159E-4	0.0	0.43910765969007176	3.110951711871017E-4
56-57	2.2855971760685022E-4	6.920280338651854E-4	0.0	0.5141069914703418	3.301418143210059E-4
58-59	2.3490859865148497E-4	7.14249117521407E-4	0.0	0.572834141133213	3.39665135887958E-4
60-61	2.412574796961197E-4	7.459935227445806E-4	0.0	0.6087656334053233	3.491884574549101E-4
62-63	2.412574796961197E-4	7.555168443115327E-4	0.0	0.6321644344953246	3.74583981633449E-4
64-65	2.412574796961197E-4	8.317034168471495E-4	0.0	0.6467541631358952	3.74583981633449E-4
66-67	2.412574796961197E-4	8.380522978917842E-4	0.0	0.6547442299305679	3.9363062476735315E-4
68-69	2.476063607407544E-4	8.729711436372752E-4	0.0	0.6629533331212807	4.0632838685662265E-4
70-71	2.476063607407544E-4	8.88843346248862E-4	0.0	0.6703434306572356	4.44421673124431E-4
72-73	2.5395524178538917E-4	8.920177867711794E-4	0.0	0.6756828396157732	4.7934051886992205E-4
74-75	2.6347856335234127E-4	9.047155488604489E-4	0.0	0.6803365694214906	4.8568939991455674E-4
76-77	2.730018849192933E-4	9.650299187844787E-4	0.0	0.6844093766116237	4.888638404368741E-4
78-79	2.730018849192933E-4	9.935998834853352E-4	0.0	0.6878504701378159	4.888638404368741E-4
80-81	2.730018849192933E-4	9.999487645299698E-4	0.0	0.6896948200812822	5.237826861823651E-4
82-83	2.7935076596392807E-4	0.001003123205052287	0.0	0.691174109364682	5.650504129724909E-4
84-85	2.920485280531975E-4	0.0010094720860969219	0.0	0.692332780155328	5.713992940171256E-4
86-87	2.920485280531975E-4	0.0010094720860969219	0.0	0.6925359443487562	5.777481750617603E-4
88-89	2.920485280531975E-4	0.001088833099154856	0.0	0.6925994331592026	5.872714966287124E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15730	0.0	49.44763	1
GTATCAA	29470	0.0	35.937084	1
ATCAACG	35075	0.0	29.343931	3
TCAACGC	36475	0.0	28.229305	4
CAACGCA	37520	0.0	27.420387	5
AACGCAG	38290	0.0	26.980179	6
TATCAAC	39115	0.0	26.542244	2
ACGCAGA	39475	0.0	26.106089	7
CGCAGAG	46540	0.0	22.115627	8
GTACATG	44310	0.0	20.012163	1
ACATGGG	42915	0.0	19.39277	3
TACATGG	44670	0.0	19.34573	2
AGAGTAC	49130	0.0	18.529171	10-11
GCAGAGT	55405	0.0	18.33898	9
GAGTACT	29215	0.0	17.345934	12-13
CAGAGTA	53960	0.0	16.365952	10-11
CATGGGG	31580	0.0	16.288996	4
AGTACTT	33285	0.0	16.036661	12-13
GTACTTT	33740	0.0	15.133465	14-15
GTGGTAT	8510	0.0	14.049952	1
>>END_MODULE