FastQCFastQC Report
Fri 27 May 2016
SRR2139675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12714418
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145750.048.0958181
GTATCAA267600.036.7884751
TCAACGC331600.029.071274
ATCAACG337750.028.4789123
CAACGCA339650.028.3947875
AACGCAG349600.027.6353366
TATCAAC359000.027.0428892
ACGCAGA406550.023.7329257
CGCAGAG416000.023.2449558
GTACATG402850.019.7041531
GCAGAGT489450.019.4523189
TACATGG404250.019.2358252
AGAGTAC444800.018.91714710-11
ACATGGG411750.018.3784313
GAGTACT256050.017.14601912-13
CAGAGTA483600.016.93734610-11
CATGGGG269850.016.8129964
AGTACTT286850.016.53651812-13
TATACTG79550.014.873565
GTACTTT296450.014.85997214-15
Produced by FastQC (version 0.11.5)