##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12714418
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.642844525010897	31.0	31.0	34.0	30.0	34.0
2	31.88213695664245	31.0	31.0	34.0	30.0	34.0
3	31.891727564722192	31.0	31.0	34.0	30.0	34.0
4	35.64319216184335	37.0	35.0	37.0	33.0	37.0
5	35.47322795270692	37.0	35.0	37.0	33.0	37.0
6	35.469167523043524	37.0	35.0	37.0	33.0	37.0
7	35.33598981880256	37.0	35.0	37.0	32.0	37.0
8	35.31055994855604	37.0	35.0	37.0	32.0	37.0
9	36.82960863800451	39.0	37.0	39.0	33.0	39.0
10-11	36.75305137836431	39.0	35.0	39.0	32.0	39.0
12-13	36.68607674374084	39.0	35.0	39.0	32.0	39.0
14-15	37.7407214785608	39.0	37.0	41.0	32.0	41.0
16-17	37.79146233826826	40.0	37.0	41.0	32.0	41.0
18-19	37.83434802127789	40.0	37.0	41.0	32.0	41.0
20-21	37.81777032151109	40.0	37.0	41.0	32.0	41.0
22-23	37.74636009158701	39.5	37.0	41.0	32.0	41.0
24-25	37.65156239576504	39.0	37.0	41.0	32.0	41.0
26-27	37.48745844948389	39.0	36.0	41.0	32.0	41.0
28-29	37.349903014002535	39.0	36.0	41.0	31.5	41.0
30-31	37.204344597395206	39.0	36.0	40.0	31.0	41.0
32-33	37.41185157052138	39.0	36.0	41.0	32.0	41.0
34-35	37.4114723486274	39.0	36.0	41.0	31.5	41.0
36-37	37.297455253974555	39.0	36.0	41.0	31.0	41.0
38-39	37.18406864807011	39.0	36.0	41.0	31.0	41.0
40-41	37.166298760459085	39.0	36.0	40.5	31.0	41.0
42-43	37.097641481685656	39.0	36.0	40.0	31.0	41.0
44-45	37.07228063456745	39.0	36.0	40.0	31.0	41.0
46-47	37.08519765207805	39.0	36.0	40.0	31.0	41.0
48-49	37.00956320516284	39.0	35.5	40.0	31.0	41.0
50-51	36.931002004366704	39.0	35.0	40.0	30.5	41.0
52-53	36.80625441157689	39.0	35.0	40.0	30.0	41.0
54-55	36.70508813354333	39.0	35.0	40.0	30.0	41.0
56-57	36.53352754457469	38.0	35.0	40.0	30.0	41.0
58-59	36.39321559981555	38.0	35.0	40.0	30.0	41.0
60-61	36.235168557029674	38.0	34.5	40.0	29.5	41.0
62-63	36.00923794488358	38.0	34.0	40.0	29.0	41.0
64-65	35.80818640883744	37.5	34.0	40.0	29.0	41.0
66-67	35.55986140705395	37.0	34.0	40.0	29.0	41.0
68-69	35.29099823083142	36.5	34.0	39.0	28.0	41.0
70-71	35.0115260198198	36.0	33.5	39.0	28.0	40.0
72-73	34.71075045741944	36.0	33.0	39.0	28.0	40.0
74-75	34.3890241022972	35.0	33.0	38.0	28.0	40.0
76-77	33.32480168741902	34.5	31.5	37.0	26.5	39.0
78-79	33.69323586832361	35.0	32.0	37.0	28.0	39.0
80-81	33.613778224488755	35.0	32.0	36.5	28.0	39.0
82-83	33.41474973712597	35.0	32.0	36.0	28.0	37.5
84-85	33.17657676148401	35.0	32.0	36.0	27.5	37.0
86-87	32.99828009473818	34.5	32.0	35.0	27.0	37.0
88-89	32.800233156391684	34.0	32.0	35.0	27.0	36.0
90-91	32.69780795270506	34.0	32.0	35.0	27.0	36.0
92-93	32.65172420083118	34.0	32.0	35.0	27.0	36.0
94-95	32.636449340853545	34.0	32.0	35.0	27.0	35.5
96-97	32.545701399877046	34.0	32.0	35.0	27.0	35.0
98-99	32.68504339016154	34.0	32.0	35.0	28.5	35.0
100-101	32.24533192244575	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	5.0
19	17.0
20	114.0
21	748.0
22	3545.0
23	11567.0
24	29838.0
25	62814.0
26	109916.0
27	169685.0
28	238602.0
29	311628.0
30	390426.0
31	478340.0
32	582047.0
33	719907.0
34	914859.0
35	1187758.0
36	1654591.0
37	2380449.0
38	2462859.0
39	975672.0
40	29028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.70224622157302	23.111816836602355	16.20222018813602	23.983716753688608
2	13.413947747739527	23.452206546862193	41.0139486286625	22.119897076735782
3	16.027088302429572	28.83397415438127	32.15946652060676	22.979471022582395
4	11.616457788315596	20.490894667770085	40.200959257435144	27.691688286479177
5	12.06227449813275	39.02664675646184	36.65699051266051	12.254088232744904
6	27.55995594922237	40.557145439138466	19.591624248943205	12.291274362695956
7	24.506619178321806	33.599351539331174	25.034610314054483	16.859418968292534
8	23.587332113825425	37.67532261405909	21.973235424539293	16.764109847576194
9	25.138397998241054	16.7696783289648	21.90374738348228	36.18817628931187
10-11	23.155365821699426	27.582402906684365	30.085085294505813	19.177145977110396
12-13	24.280961320816267	25.153162935696244	29.971434963546418	20.594440779941074
14-15	21.45771045123733	26.363397050498104	27.479578695619416	24.699313802645154
16-17	20.937824287356293	29.446404861001106	28.074399473102112	21.54137137854049
18-19	21.026360623771787	28.72673944342388	29.652928304736342	20.59397162806799
20-21	21.9433606842435	27.896426070918878	29.360708705039585	20.79950453979804
22-23	21.646535968031703	27.856834601757978	29.248147472792446	21.248481957417873
24-25	21.374304793835712	28.048409863367834	29.267327284231403	21.309958058565048
26-27	21.40563550341886	28.108120915442335	29.16929759078523	21.31694599035357
28-29	21.363270594489784	28.107100084020768	29.204929769871914	21.32469955161753
30-31	21.603571479807872	28.015099301749718	29.094666979655663	21.286662238786747
32-33	21.42894965041443	28.169615387759094	29.074521723813206	21.32691323801327
34-35	21.527942045892473	28.171637301069335	29.100706091647517	21.199714561390675
36-37	21.511185897881624	28.15315173922286	29.01937988346243	21.316282479433085
38-39	21.417883545873202	28.1894351245292	29.076428675137638	21.316252654459962
40-41	21.578272317008796	28.070945354667497	28.99031651810195	21.36046581022176
42-43	21.474106628892674	28.204816572741453	28.772057644289955	21.549019154075918
44-45	21.56664976662941	28.284402342253813	28.601857144441812	21.547090746674964
46-47	21.638446422175274	28.26361877434015	28.399393053655164	21.69854174982941
48-49	21.59072222077073	28.356486091916892	28.356211946485317	21.69657974082706
50-51	21.588989609935908	28.431002321225986	28.241427781280272	21.738580287557838
52-53	21.72012496644479	28.328972493427905	28.17635858267522	21.774543957452078
54-55	21.687737755746635	28.284784321717098	28.19682177136751	21.830656151168753
56-57	21.68325294846567	28.373104300267094	28.19118253482381	21.752460216443424
58-59	21.65189098527847	28.411797694720054	28.191998356711682	21.74431296328979
60-61	21.774503854638137	28.355461483712997	28.184109265035705	21.685925396613158
62-63	21.714059611501067	28.422764414233793	28.160384301594128	21.70279167267101
64-65	21.659647996521443	28.383916393437875	28.152361536544124	21.804074073496558
66-67	21.703167120982517	28.443811417977138	28.01178420501957	21.84123725602078
68-69	21.66036304613766	28.407960110723874	28.06409896445362	21.867577878684845
70-71	21.682612982913884	28.208368819709328	28.14914661479304	21.959871582583748
72-73	21.71185796501145	28.252554955759834	28.069098590902552	21.966488488326167
74-75	21.596364861997078	28.28773590794011	28.094965436558788	22.020933793504017
76-77	21.729974054297248	28.25104802719247	28.033531151047452	21.98544676746283
78-79	21.70701202667323	28.221879399956062	28.00088432768973	22.07022424568098
80-81	21.692770654492126	28.28015236663261	27.994280309160263	22.032796669714998
82-83	21.70852678488188	28.20485393292304	28.113429355917923	21.973189926277158
84-85	21.729346956332705	28.21303308142971	28.067593077279025	21.99002688495856
86-87	21.657156724392493	28.235178357301923	28.15544677015442	21.95221814815117
88-89	21.689837989321223	28.156904887061586	28.133136064186388	22.020121059430807
90-91	21.721737987574492	28.15812775271478	28.189126235595207	21.931008024115524
92-93	21.712887164706085	28.0674887219143	28.229054805395144	21.99056930798447
94-95	21.513005839401245	28.08374882374092	28.304286259291793	22.09895907756604
96-97	21.47099996024553	28.159049456533847	28.37487957356142	21.99507100965921
98-99	21.636083852672215	28.782950980987877	28.460685992054923	21.12027917428499
100-101	22.187926604598797	31.516635462858776	25.708136606081762	20.587301326460665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2223.0
1	2110.5
2	3238.5
3	4717.0
4	6048.5
5	8810.0
6	11112.5
7	13407.0
8	15328.5
9	15401.0
10	14234.5
11	13171.0
12	14507.0
13	19384.5
14	31769.0
15	49395.5
16	62901.5
17	69241.5
18	70128.0
19	67452.0
20	63403.5
21	60808.0
22	62207.5
23	69714.0
24	84417.0
25	106076.5
26	132904.5
27	162267.5
28	194971.5
29	229991.0
30	262837.0
31	294947.0
32	328003.5
33	360361.5
34	389915.5
35	417336.5
36	445525.0
37	470739.5
38	489306.0
39	500502.5
40	509726.5
41	519555.5
42	523973.0
43	526668.5
44	528547.0
45	527748.0
46	523214.0
47	514937.0
48	505326.0
49	490057.5
50	471541.5
51	449835.0
52	422464.0
53	393738.5
54	364673.0
55	335325.5
56	305670.5
57	273884.0
58	241015.0
59	205426.0
60	168781.5
61	135784.5
62	106858.0
63	82061.5
64	60201.0
65	43475.5
66	32253.5
67	24329.5
68	18226.5
69	12911.0
70	8911.5
71	6293.0
72	4276.0
73	2779.5
74	1840.5
75	1232.0
76	852.0
77	580.5
78	397.5
79	287.0
80	216.0
81	164.0
82	125.0
83	84.0
84	56.5
85	41.0
86	29.5
87	26.5
88	20.5
89	12.5
90	9.0
91	7.5
92	4.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003775241619396185
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.747110406469255E-4
14-15	0.0
16-17	0.0
18-19	4.443773989497592E-4
20-21	0.0
22-23	0.0014238206772587913
24-25	1.7703582544173192E-4
26-27	0.005139901952237874
28-29	4.094908158887949E-4
30-31	0.0
32-33	3.948624603232329E-6
34-35	2.1374600562151994E-4
36-37	0.021121696765448703
38-39	0.010140491537861656
40-41	0.01378657672027024
42-43	0.0015818931733379876
44-45	0.002919901871722349
46-47	0.0
48-49	6.521295769008227E-4
50-51	4.189861214618141E-5
52-53	4.223437230029974E-6
54-55	0.004383677127113358
56-57	0.009068012194309177
58-59	0.011529227066849904
60-61	0.006132146846690997
62-63	0.006920056968662866
64-65	8.375555264787164E-4
66-67	1.1250813208778731E-4
68-69	0.0
70-71	8.284750177500772E-5
72-73	0.0
74-75	1.3189888273638177E-4
76-77	4.289331826475176E-5
78-79	9.650017507544264E-6
80-81	0.001995337658814573
82-83	0.002057952844713176
84-85	0.009108966158481533
86-87	0.010404452840734234
88-89	0.04363480289103603
90-91	4.896967539687157E-4
92-93	7.086416976048715E-4
94-95	0.0
96-97	0.01017159836011732
98-99	1.358171414696147E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	354.0
20-21	1272.0
22-23	2431.0
24-25	4487.0
26-27	5495.0
28-29	9058.0
30-31	22246.0
32-33	28202.0
34-35	40533.0
36-37	62289.0
38-39	74937.0
40-41	82470.0
42-43	93407.0
44-45	111164.0
46-47	111785.0
48-49	105618.0
50-51	97902.0
52-53	89177.0
54-55	95454.0
56-57	101038.0
58-59	106474.0
60-61	113386.0
62-63	104930.0
64-65	110457.0
66-67	119746.0
68-69	124755.0
70-71	128133.0
72-73	123006.0
74-75	123016.0
76-77	124424.0
78-79	137589.0
80-81	141703.0
82-83	137280.0
84-85	136625.0
86-87	145317.0
88-89	159388.0
90-91	179180.0
92-93	190490.0
94-95	235109.0
96-97	306927.0
98-99	1069736.0
100-101	7557428.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.55561156283613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40601672346753	75.59780421449952
2	7.32528673109667	12.387881988420155
3	1.8224001143198794	4.622824685354996
4	0.6720773401715439	2.273116420629166
5	0.3035931246480456	1.2835251160443917
6	0.1635052406758976	0.829517137144754
7	0.09073083523271058	0.5370260882496134
8	0.05697272481977288	0.3853890871629655
9	0.03775082399044778	0.28728396085619684
>10	0.11817026900965914	1.5497713315169697
>50	0.002880362059983003	0.15875742979197557
>100	5.999977761620803E-4	0.08019382219294834
>500	1.5712731740166135E-5	0.006908718136385178
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.573017341415077E-5	0.0	7.865086707075385E-6	0.0
3	0.0	1.573017341415077E-5	0.0	7.865086707075385E-6	0.0
4	0.0	7.078578036367847E-5	0.0	7.865086707075385E-6	0.0
5	0.0	7.078578036367847E-5	0.0	7.865086707075385E-6	0.0
6	0.0	7.078578036367847E-5	0.0	7.865086707075385E-6	0.0
7	0.0	7.865086707075385E-5	0.0	1.573017341415077E-5	0.0
8	0.0	8.651595377782924E-5	0.0	1.573017341415077E-5	0.0
9	0.0	1.6516682084858307E-4	0.0	3.146034682830154E-5	0.0
10-11	0.0	1.6516682084858307E-4	0.0	4.719052024245231E-5	0.0
12-13	0.0	1.6516682084858307E-4	0.0	5.505560694952769E-5	0.0
14-15	0.0	1.966271676768846E-4	0.0	5.505560694952769E-5	0.0
16-17	0.0	2.005597110304223E-4	0.0	5.898815030306539E-5	0.0
18-19	0.0	2.202224277981108E-4	0.0	7.865086707075386E-5	7.865086707075385E-6
20-21	2.3595260121226154E-5	2.4381768791933693E-4	0.0	9.438104048490462E-5	7.865086707075385E-6
22-23	2.3595260121226154E-5	2.831431214547138E-4	0.0	1.4943664743443232E-4	7.865086707075385E-6
24-25	2.3595260121226154E-5	3.1853601163655306E-4	0.0	4.6404011571744765E-4	3.146034682830154E-5
26-27	2.3595260121226154E-5	3.3033364169716614E-4	0.0	0.001368525087031117	3.146034682830154E-5
28-29	3.146034682830154E-5	3.3033364169716614E-4	0.0	0.007585876128974208	3.146034682830154E-5
30-31	3.146034682830154E-5	3.814567052931562E-4	0.0	0.01169145139006756	3.146034682830154E-5
32-33	3.146034682830154E-5	4.011194220608446E-4	0.0	0.014538612778028849	3.146034682830154E-5
34-35	3.146034682830154E-5	4.561750290103723E-4	0.0	0.018585199888819133	3.146034682830154E-5
36-37	3.146034682830154E-5	5.112306359599E-4	0.0	0.023626720468054453	3.146034682830154E-5
38-39	3.146034682830154E-5	5.308933527275884E-4	0.0	0.03472829035509136	3.146034682830154E-5
40-41	3.146034682830154E-5	5.426909827882016E-4	0.0	0.060466786603995556	3.146034682830154E-5
42-43	3.146034682830154E-5	5.584211562023524E-4	0.0	0.07130487608634545	3.146034682830154E-5
44-45	3.146034682830154E-5	6.488696533337192E-4	0.0	0.08716089088780941	3.146034682830154E-5
46-47	3.146034682830154E-5	7.314530637580108E-4	0.0	0.10446408164337526	3.146034682830154E-5
48-49	3.9325433535376924E-5	7.314530637580108E-4	0.0	0.11998583025978854	3.146034682830154E-5
50-51	3.9325433535376924E-5	7.6684595393985E-4	0.0	0.13623116685325273	3.146034682830154E-5
52-53	4.325797688891462E-5	7.786435840004631E-4	0.0	0.15796633396825555	3.539289018183923E-5
54-55	5.112306359599E-5	7.983063007681515E-4	0.0	0.2016647557127664	4.325797688891462E-5
56-57	5.505560694952769E-5	8.101039308287646E-4	0.0	0.2464131665326718	5.505560694952769E-5
58-59	5.505560694952769E-5	8.101039308287646E-4	0.0	0.2926992018038105	5.505560694952769E-5
60-61	5.505560694952769E-5	8.179690175358401E-4	0.0	0.3231646151636669	5.505560694952769E-5
62-63	7.078578036367847E-5	8.376317343035285E-4	0.0	0.33789592256601914	6.292069365660308E-5
64-65	7.078578036367847E-5	9.595405782631969E-4	0.0	0.35223004308966405	6.292069365660308E-5
66-67	7.078578036367847E-5	9.674056649702724E-4	0.0	0.359214240085547	6.292069365660308E-5
68-69	7.078578036367847E-5	0.0010067310985056492	0.0	0.3658366430929044	6.292069365660308E-5
70-71	7.865086707075385E-5	0.0010460565320410263	0.0	0.3722820816493527	6.292069365660308E-5
72-73	7.865086707075385E-5	0.0010578541621016394	0.0	0.3773039395118204	6.292069365660308E-5
74-75	7.865086707075385E-5	0.0010932470522834784	0.0	0.38171625315448965	6.292069365660308E-5
76-77	7.865086707075385E-5	0.00117583046270777	0.0	0.3859280070861285	6.292069365660308E-5
78-79	7.865086707075385E-5	0.0012033582661825338	0.0	0.38960493512168626	6.292069365660308E-5
80-81	7.865086707075385E-5	0.0012033582661825338	0.0	0.391504353561445	6.685323701014078E-5
82-83	7.865086707075385E-5	0.0012072908095360716	0.0	0.39295939460225393	7.078578036367847E-5
84-85	7.865086707075385E-5	0.00122695352630376	0.0	0.39443016581647705	7.078578036367847E-5
86-87	7.865086707075385E-5	0.0012348186130108354	0.0	0.39462286044080036	7.078578036367847E-5
88-89	7.865086707075385E-5	0.00127807658989975	0.0	0.39465432078762863	7.078578036367847E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14575	0.0	48.095818	1
GTATCAA	26760	0.0	36.788475	1
TCAACGC	33160	0.0	29.07127	4
ATCAACG	33775	0.0	28.478912	3
CAACGCA	33965	0.0	28.394787	5
AACGCAG	34960	0.0	27.635336	6
TATCAAC	35900	0.0	27.042889	2
ACGCAGA	40655	0.0	23.732925	7
CGCAGAG	41600	0.0	23.244955	8
GTACATG	40285	0.0	19.704153	1
GCAGAGT	48945	0.0	19.452318	9
TACATGG	40425	0.0	19.235825	2
AGAGTAC	44480	0.0	18.917147	10-11
ACATGGG	41175	0.0	18.378431	3
GAGTACT	25605	0.0	17.146019	12-13
CAGAGTA	48360	0.0	16.937346	10-11
CATGGGG	26985	0.0	16.812996	4
AGTACTT	28685	0.0	16.536518	12-13
TATACTG	7955	0.0	14.87356	5
GTACTTT	29645	0.0	14.859972	14-15
>>END_MODULE