FastQCFastQC Report
Fri 27 May 2016
SRR2139674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9721023
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109550.049.8262941
GTATCAA197850.035.612471
TCAACGC250600.027.4716134
ATCAACG252900.027.2385563
CAACGCA257500.026.7519655
TATCAAC266000.026.2490922
AACGCAG265900.025.986666
ACGCAGA306450.022.4791557
CGCAGAG310400.022.1930968
GTACATG277250.019.749141
TACATGG279950.019.3312422
AGAGTAC328100.018.81605510-11
GCAGAGT365800.018.6811359
ACATGGG285950.018.3757443
GAGTACT200550.017.59807412-13
CAGAGTA360650.016.77065510-11
AGTACTT227500.016.11040712-13
CATGGGG207800.015.8704734
GTACTTT228200.015.55883114-15
TATACTG54800.014.3286595
Produced by FastQC (version 0.11.5)