##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9721023
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.605950423119047	31.0	31.0	34.0	30.0	34.0
2	31.851544225335132	31.0	31.0	34.0	30.0	34.0
3	31.862135497467705	31.0	31.0	34.0	30.0	34.0
4	35.620249329725894	37.0	35.0	37.0	33.0	37.0
5	35.44689051759264	37.0	35.0	37.0	33.0	37.0
6	35.43676555440719	37.0	35.0	37.0	33.0	37.0
7	35.30200350312925	37.0	35.0	37.0	32.0	37.0
8	35.277575621413504	37.0	35.0	37.0	32.0	37.0
9	36.79150887720356	39.0	37.0	39.0	32.0	39.0
10-11	36.71139390370746	39.0	35.0	39.0	32.0	39.0
12-13	36.64283774454602	39.0	35.0	39.0	32.0	39.0
14-15	37.687518021508644	39.0	37.0	41.0	32.0	41.0
16-17	37.733309498393325	39.0	37.0	41.0	32.0	41.0
18-19	37.769877408992855	39.5	37.0	41.0	32.0	41.0
20-21	37.748143039545425	39.5	37.0	41.0	32.0	41.0
22-23	37.67838357650359	39.0	37.0	41.0	32.0	41.0
24-25	37.580116847249315	39.0	36.0	41.0	32.0	41.0
26-27	37.405043818239506	39.0	36.0	41.0	32.0	41.0
28-29	37.25976520873158	39.0	36.0	40.5	31.0	41.0
30-31	37.111333452773906	39.0	36.0	40.0	31.0	41.0
32-33	37.328139496710534	39.0	36.0	40.5	31.0	41.0
34-35	37.328774184707015	39.0	36.0	41.0	31.0	41.0
36-37	37.21401338579444	39.0	36.0	41.0	31.0	41.0
38-39	37.098850572843986	39.0	36.0	40.0	31.0	41.0
40-41	37.07680895798021	39.0	36.0	40.0	31.0	41.0
42-43	37.00097289544317	39.0	35.0	40.0	31.0	41.0
44-45	36.97144229619908	39.0	35.0	40.0	30.5	41.0
46-47	36.983525829289874	39.0	35.0	40.0	31.0	41.0
48-49	36.902827789036635	39.0	35.0	40.0	30.0	41.0
50-51	36.8171825649717	39.0	35.0	40.0	30.0	41.0
52-53	36.68745254526908	39.0	35.0	40.0	30.0	41.0
54-55	36.57407591264014	38.5	35.0	40.0	30.0	41.0
56-57	36.400123042260304	38.0	35.0	40.0	30.0	41.0
58-59	36.250972913315856	38.0	34.5	40.0	29.0	41.0
60-61	36.08543485189037	38.0	34.0	40.0	29.0	41.0
62-63	35.85565304151333	38.0	34.0	40.0	29.0	41.0
64-65	35.64027146489404	37.0	34.0	40.0	29.0	41.0
66-67	35.384293592223244	37.0	34.0	39.5	28.0	41.0
68-69	35.105762091094675	36.0	33.5	39.0	28.0	40.5
70-71	34.821194335134415	36.0	33.0	39.0	28.0	40.0
72-73	34.51512494188782	35.5	33.0	38.5	28.0	40.0
74-75	34.19713692202753	35.0	33.0	37.5	28.0	40.0
76-77	33.15728726768507	34.5	31.5	36.0	26.0	39.0
78-79	33.52749834788824	35.0	32.0	36.5	27.0	39.0
80-81	33.45874110277083	35.0	32.0	36.0	28.0	39.0
82-83	33.26974503526476	35.0	32.0	36.0	27.5	37.5
84-85	33.041916538556386	34.5	32.0	35.5	27.0	37.0
86-87	32.87175414422203	34.0	32.0	35.0	27.0	37.0
88-89	32.68411870109314	34.0	32.0	35.0	27.0	36.0
90-91	32.58697754304572	34.0	32.0	35.0	27.0	36.0
92-93	32.55397290877052	34.0	32.0	35.0	27.0	36.0
94-95	32.54333823689896	34.0	32.0	35.0	27.0	35.0
96-97	32.45154121744331	34.0	32.0	35.0	27.0	35.0
98-99	32.601534451358674	34.0	32.0	35.0	28.0	35.0
100-101	32.16423433927352	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	15.0
20	84.0
21	592.0
22	2837.0
23	9586.0
24	24565.0
25	50415.0
26	88987.0
27	136332.0
28	191596.0
29	247954.0
30	310752.0
31	378858.0
32	461005.0
33	566109.0
34	716918.0
35	932359.0
36	1295562.0
37	1827037.0
38	1785257.0
39	675162.0
40	19038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.67193257335159	22.889566252440716	16.235297457891008	24.203203716316686
2	13.721024899706471	23.271009741359613	40.50458417830701	22.503381180626903
3	16.291999309126208	28.325979683413976	31.95498045833242	23.427040549127394
4	11.948793866653746	20.239947997242677	39.55620720164946	28.255050934454122
5	12.38768800361855	38.724165141878586	36.32942746869337	12.558719385809496
6	28.269607015640226	40.0733338456251	19.13811951684509	12.518939621889588
7	24.961590976587548	33.303305629458954	24.59128015641975	17.143823237533745
8	23.91428350699304	37.45859875035786	21.58948703238332	17.03763071026578
9	25.25268173936015	16.636119470142184	21.529987121725767	36.58121166877189
10-11	23.58750205611076	27.305834992880897	29.66515458301045	19.441508367997894
12-13	24.62416051701181	24.810788587953724	29.601462862870697	20.963588032163766
14-15	21.733664245007958	26.005493454752653	27.100403938968153	25.160438361271236
16-17	21.32004522569281	29.07457887919821	27.692394102966322	21.91298179214266
18-19	21.419011842732367	28.38175419090847	29.230546951887483	20.968687014471683
20-21	22.30309765159432	27.569463879883223	28.953831787805523	21.173606680716937
22-23	22.007388452982852	27.456956422428963	28.878469671434537	21.657185453153648
24-25	21.747634014210323	27.66466190793753	28.901164777171275	21.686539300680877
26-27	21.796706890748585	27.72042291348708	28.78118673963363	21.70168345613071
28-29	21.796499905972375	27.76080278525744	28.802405335350517	21.64029197341967
30-31	22.039163529350013	27.657834948836136	28.65458331825132	21.64841820356253
32-33	21.83279081112886	27.831345175914464	28.64314203869696	21.692721974259715
34-35	21.907581406642475	27.80179897222897	28.676335617545202	21.614284003583357
36-37	21.940246563303035	27.774383143255466	28.634271713618958	21.651098579822538
38-39	21.82471505656205	27.844373272923516	28.662400767280488	21.668510903233944
40-41	21.99433148752433	27.71087881519364	28.56784941263343	21.726940284648595
42-43	21.898663386131563	27.873265168544805	28.35264918647046	21.87542225885317
44-45	21.9935214019143	27.89881628893366	28.2053719119312	21.902290397220842
46-47	22.059169275509618	27.898428879919802	27.97746140914358	22.064940435427005
48-49	21.978041659755203	28.049806527096738	27.946503390804594	22.025648422343462
50-51	22.01427549255513	28.084710057960066	27.826423842034377	22.07459060745043
52-53	22.15056084466459	27.99401186828037	27.740284888124283	22.115142398930757
54-55	22.09808042914124	27.883259216774352	27.83397846119417	22.184681892890236
56-57	22.121054338935608	27.991166555322778	27.79818988525739	22.089589220484225
58-59	22.081383524812505	28.067074607355526	27.756333604198126	22.095208263633843
60-61	22.16686085606804	28.014421506040737	27.776737124209056	22.04198051368217
62-63	22.13204911655974	28.09020266681264	27.723878935260466	22.05386928136715
64-65	22.098815908221294	28.053568643649868	27.702750485546474	22.14486496258236
66-67	22.145440482160257	28.063494053419873	27.629037713712616	22.16202775070725
68-69	22.100154713254405	28.03744323793081	27.686624357653045	22.175777691161734
70-71	22.09726005213362	27.818116245174835	27.757596449795603	22.327027252895945
72-73	22.14945108406645	27.877565843360063	27.69889071530497	22.274092357268522
74-75	21.97989899622455	27.987743706942027	27.72757965439511	22.304777642438317
76-77	22.11190551494221	27.941564615782582	27.658381394472965	22.28814847480224
78-79	22.068294612012558	27.93834713121689	27.637567423516966	22.355790833253586
80-81	22.119513512784327	27.92398846028582	27.63487249720725	22.321625529722603
82-83	22.129640129378423	27.842766096569026	27.77774991514744	22.249843858905116
84-85	22.129322337059328	27.85957330609029	27.75768926357609	22.25341509327429
86-87	22.041052096856884	27.890070096704854	27.801766933897866	22.267110872540396
88-89	22.069700658074186	27.846416071631907	27.756193261286594	22.327690009007316
90-91	22.116162930187116	27.825337010908136	27.81295421596466	22.245545842940086
92-93	22.106871153016368	27.756674429968015	27.818255089098326	22.31819932791729
94-95	21.90830579344961	27.8094664152785	27.91482021192585	22.367407579346043
96-97	21.85262134058171	27.873255198033657	27.979393572315804	22.29472988906883
98-99	22.010309014063807	28.51412199134628	28.041941429474566	21.433627565115348
100-101	22.582390848576807	31.211268252529788	25.27090365027368	20.935437248619724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1843.0
1	1717.5
2	2519.5
3	3604.0
4	4613.0
5	6747.5
6	8535.0
7	10313.0
8	11848.0
9	12104.5
10	11271.5
11	10209.0
12	11183.5
13	15069.0
14	24488.5
15	37773.0
16	48123.5
17	52681.5
18	52587.5
19	50135.0
20	47005.5
21	44705.5
22	44933.0
23	49903.5
24	60004.0
25	75549.0
26	95312.5
27	115874.5
28	137350.5
29	160095.5
30	181343.5
31	203286.5
32	227414.0
33	250792.0
34	272669.0
35	293136.0
36	315549.0
37	336340.5
38	350783.0
39	361140.5
40	371931.5
41	382586.0
42	388260.5
43	393278.5
44	398769.5
45	402333.5
46	402606.5
47	399453.5
48	395549.5
49	388111.0
50	377639.0
51	365550.0
52	348970.5
53	329446.0
54	309173.5
55	286184.5
56	261704.0
57	236038.0
58	208437.0
59	179953.5
60	150877.5
61	123027.5
62	97291.5
63	74901.0
64	56133.5
65	41800.0
66	31454.5
67	23892.0
68	18305.0
69	13286.0
70	9373.0
71	6644.5
72	4565.0
73	3100.0
74	2081.5
75	1389.5
76	951.0
77	643.5
78	451.0
79	323.5
80	236.5
81	174.5
82	135.0
83	96.0
84	63.5
85	42.5
86	27.5
87	19.0
88	13.5
89	7.0
90	4.0
91	4.0
92	3.5
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0031478168501401552
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.6925007789818E-4
14-15	0.0
16-17	0.0
18-19	4.114793268157065E-4
20-21	0.0
22-23	0.0017079268175993832
24-25	2.212639492126168E-4
26-27	0.005554479950618151
28-29	3.760060737857837E-4
30-31	0.0
32-33	5.166303837148184E-6
34-35	2.278958151491464E-4
36-37	0.020389648151718943
38-39	0.008260214748853922
40-41	0.012896389011076898
42-43	0.0018863851424067116
44-45	0.0029971339706062897
46-47	5.393607108515276E-6
48-49	7.022560601976073E-4
50-51	2.7460889788849373E-5
52-53	2.215036766010881E-5
54-55	0.004466671594894326
56-57	0.009328804882074555
58-59	0.011838625545809254
60-61	0.006362193229455026
62-63	0.007114659021413732
64-65	9.19099299078483E-4
66-67	1.0052879922429613E-4
68-69	0.0
70-71	9.675417557762544E-5
72-73	0.0
74-75	1.547009312315376E-4
76-77	5.6343625498195034E-5
78-79	0.0
80-81	0.0021455031410422933
82-83	0.0020321214572942186
84-85	0.009376573423687404
86-87	0.01082827098829636
88-89	0.04191725513438781
90-91	5.119118072804236E-4
92-93	5.854713382859036E-4
94-95	1.4417212652516989E-5
96-97	0.009458668398877976
98-99	1.1652627710274968E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	276.0
20-21	971.0
22-23	1875.0
24-25	3837.0
26-27	5057.0
28-29	8008.0
30-31	17766.0
32-33	22468.0
34-35	32246.0
36-37	49573.0
38-39	60679.0
40-41	64963.0
42-43	73449.0
44-45	87143.0
46-47	87279.0
48-49	82207.0
50-51	75884.0
52-53	73039.0
54-55	78153.0
56-57	82290.0
58-59	84199.0
60-61	86171.0
62-63	81994.0
64-65	83815.0
66-67	90510.0
68-69	94427.0
70-71	96963.0
72-73	92761.0
74-75	93423.0
76-77	94191.0
78-79	104778.0
80-81	107665.0
82-83	105116.0
84-85	104405.0
86-87	110003.0
88-89	122476.0
90-91	137172.0
92-93	146482.0
94-95	181106.0
96-97	236177.0
98-99	822499.0
100-101	5737527.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.67293194411441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.42293451135238	77.46797914578207
2	6.598003982677995	11.305406923499357
3	1.6501358049349824	4.241159175442236
4	0.6124248823324714	2.098729410598084
5	0.26261915029237	1.1249676295109687
6	0.15075054570682528	0.7749144745727372
7	0.07943121543307204	0.476357357982511
8	0.05379078919787005	0.3686731697735461
9	0.03912017045452573	0.30163857308934655
>10	0.12826168771520585	1.6476163380653754
>50	0.0021625571933546516	0.1226771948951587
>100	3.25695562629743E-4	0.04747346865972785
>500	3.900714637731885E-5	0.022407138128921433
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0573966340785326E-5	0.0	0.0	0.0
3	0.0	2.0573966340785326E-5	0.0	0.0	0.0
4	0.0	9.258284853353397E-5	0.0	1.0286983170392663E-5	0.0
5	0.0	1.1315681487431929E-4	0.0	2.0573966340785326E-5	0.0
6	5.1434915851963315E-5	1.1315681487431929E-4	0.0	2.0573966340785326E-5	0.0
7	5.1434915851963315E-5	1.1315681487431929E-4	0.0	2.0573966340785326E-5	0.0
8	5.1434915851963315E-5	1.2344379804471196E-4	0.0	2.0573966340785326E-5	1.0286983170392663E-5
9	5.1434915851963315E-5	1.8516569706706793E-4	0.0	3.086094951117799E-5	1.0286983170392663E-5
10-11	5.1434915851963315E-5	1.954526802374606E-4	0.0	5.1434915851963315E-5	1.0286983170392663E-5
12-13	5.1434915851963315E-5	2.0059617182265695E-4	0.0	5.1434915851963315E-5	1.0286983170392663E-5
14-15	5.1434915851963315E-5	2.777485456006019E-4	0.0	6.172189902235598E-5	2.0573966340785326E-5
16-17	1.4916125597069362E-4	2.931790203561909E-4	0.0	7.715237377794497E-5	3.086094951117799E-5
18-19	1.5430474755588996E-4	3.291834614525652E-4	0.0	1.1830030645951562E-4	3.086094951117799E-5
20-21	1.5430474755588996E-4	3.6004441096374324E-4	0.0	1.285872896299083E-4	3.086094951117799E-5
22-23	1.645917307262826E-4	4.3205329315649187E-4	0.0	1.3373078121510463E-4	3.086094951117799E-5
24-25	1.9030918865226427E-4	4.834882090084552E-4	0.0	1.3887427280030094E-4	3.086094951117799E-5
26-27	1.954526802374606E-4	5.040621753492406E-4	0.0	2.0059617182265695E-4	3.086094951117799E-5
28-29	1.954526802374606E-4	5.092056669344369E-4	0.0	3.6518790254893956E-4	3.086094951117799E-5
30-31	2.2117013816344225E-4	5.400666164456149E-4	0.0	8.84680552653769E-4	3.086094951117799E-5
32-33	2.571745792598166E-4	5.452101080308112E-4	0.0	0.001815652529574305	4.114793268157065E-5
34-35	2.623180708450129E-4	6.326494649791488E-4	0.0	0.003111812409043781	5.1434915851963315E-5
36-37	2.6746156243020926E-4	7.200888219274865E-4	0.0	0.005374948706530167	5.1434915851963315E-5
38-39	2.6746156243020926E-4	7.406627882682717E-4	0.0	0.008898240442389654	6.172189902235598E-5
40-41	2.6746156243020926E-4	7.715237377794497E-4	0.0	0.013295925747732518	6.172189902235598E-5
42-43	2.6746156243020926E-4	7.869542125350387E-4	0.0	0.01731813616735605	6.172189902235598E-5
44-45	2.726050540154056E-4	8.84680552653769E-4	0.0	0.022302179513411294	6.172189902235598E-5
46-47	2.777485456006019E-4	9.669764180169104E-4	0.0	0.027646267270430284	6.172189902235598E-5
48-49	2.8289203718579825E-4	9.669764180169104E-4	0.0	0.03310351184232359	6.172189902235598E-5
50-51	3.137529866969762E-4	9.92693875942892E-4	0.0	0.03846817356568337	6.172189902235598E-5
52-53	3.343269530377616E-4	9.978373675280884E-4	0.0	0.044141444784154917	6.172189902235598E-5
54-55	3.394704446229579E-4	9.978373675280884E-4	0.0	0.04992787281750079	6.172189902235598E-5
56-57	3.4975742779335057E-4	9.978373675280884E-4	0.0	0.056064058278640014	6.172189902235598E-5
58-59	3.4975742779335057E-4	0.0010132678422836773	0.0	0.06189163424466746	6.172189902235598E-5
60-61	3.6518790254893956E-4	0.0010441287917948554	0.0	0.06784265400873962	6.172189902235598E-5
62-63	4.011923436453139E-4	0.0010749897413060332	0.0	0.07341819888709245	6.172189902235598E-5
64-65	4.011923436453139E-4	0.0012138640141063342	0.0	0.07956981482298725	6.172189902235598E-5
66-67	4.063358352305102E-4	0.001239581472032316	0.0	0.08574200472522285	6.172189902235598E-5
68-69	4.114793268157065E-4	0.001255011946787905	0.0	0.09185761622002128	6.172189902235598E-5
70-71	4.269098015712955E-4	0.001270442421543494	0.0	0.09745373506471489	7.200888219274864E-5
72-73	4.732012258380625E-4	0.0012807294047138865	0.0	0.10194400321859129	8.22958653631413E-5
74-75	4.732012258380625E-4	0.0013218773373954574	0.0	0.10652685422100122	8.22958653631413E-5
76-77	4.732012258380625E-4	0.001393886219588206	0.0	0.11052334718269877	8.22958653631413E-5
78-79	4.834882090084552E-4	0.0014298906606845802	0.0	0.11422151763245494	8.22958653631413E-5
80-81	4.834882090084552E-4	0.0014298906606845802	0.0	0.11636121013189661	8.22958653631413E-5
82-83	4.937751921788478E-4	0.0014350341522697766	0.0	0.11771394841880325	9.258284853353397E-5
84-85	5.040621753492406E-4	0.0014658951017809546	0.0	0.11907183019729509	9.258284853353397E-5
86-87	5.040621753492406E-4	0.0014916125597069362	0.0	0.11929300033545852	9.258284853353397E-5
88-89	5.143491585196332E-4	0.0015121865260477214	0.0	0.11933929175972528	9.258284853353397E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10955	0.0	49.826294	1
GTATCAA	19785	0.0	35.61247	1
TCAACGC	25060	0.0	27.471613	4
ATCAACG	25290	0.0	27.238556	3
CAACGCA	25750	0.0	26.751965	5
TATCAAC	26600	0.0	26.249092	2
AACGCAG	26590	0.0	25.98666	6
ACGCAGA	30645	0.0	22.479155	7
CGCAGAG	31040	0.0	22.193096	8
GTACATG	27725	0.0	19.74914	1
TACATGG	27995	0.0	19.331242	2
AGAGTAC	32810	0.0	18.816055	10-11
GCAGAGT	36580	0.0	18.681135	9
ACATGGG	28595	0.0	18.375744	3
GAGTACT	20055	0.0	17.598074	12-13
CAGAGTA	36065	0.0	16.770655	10-11
AGTACTT	22750	0.0	16.110407	12-13
CATGGGG	20780	0.0	15.870473	4
GTACTTT	22820	0.0	15.558831	14-15
TATACTG	5480	0.0	14.328659	5
>>END_MODULE