FastQCFastQC Report
Fri 27 May 2016
SRR2139673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6663531
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75100.050.369091
GTATCAA138500.035.898451
ATCAACG176550.027.5873263
TCAACGC177250.027.573544
CAACGCA179550.027.1968425
AACGCAG184400.026.4815256
TATCAAC186650.026.2084672
ACGCAGA208800.023.3468997
CGCAGAG211100.023.0925278
AGAGTAC224850.019.44857210-11
GTACATG190800.019.3172231
GCAGAGT247750.019.3019549
TACATGG194400.018.6341062
GAGTACT134700.018.22037312-13
ACATGGG197750.018.0405273
CAGAGTA247150.017.105110-11
AGTACTT152400.016.97584512-13
GTACTTT155400.016.02489514-15
CATGGGG137800.016.004714
ACTTTTT185400.013.79711116-17
Produced by FastQC (version 0.11.5)