##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139673_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6663531
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.640702954634712	31.0	31.0	34.0	30.0	34.0
2	31.88571989835419	31.0	31.0	34.0	30.0	34.0
3	31.895910891687905	31.0	31.0	34.0	30.0	34.0
4	35.64724783301826	37.0	35.0	37.0	33.0	37.0
5	35.47858140076185	37.0	35.0	37.0	33.0	37.0
6	35.47000651756554	37.0	35.0	37.0	33.0	37.0
7	35.334745047333016	37.0	35.0	37.0	32.0	37.0
8	35.308700147114195	37.0	35.0	37.0	32.0	37.0
9	36.82906855239362	39.0	37.0	39.0	33.0	39.0
10-11	36.75265621184924	39.0	35.0	39.0	32.0	39.0
12-13	36.684949090804864	39.0	35.0	39.0	32.0	39.0
14-15	37.741373079828094	39.0	37.0	41.0	32.0	41.0
16-17	37.78983072188004	39.5	37.0	41.0	32.0	41.0
18-19	37.82983443762774	40.0	37.0	41.0	32.0	41.0
20-21	37.80737425545114	40.0	37.0	41.0	32.0	41.0
22-23	37.73523833454022	39.0	37.0	41.0	32.0	41.0
24-25	37.640706446250064	39.0	36.5	41.0	32.0	41.0
26-27	37.4723750821248	39.0	36.0	41.0	32.0	41.0
28-29	37.33443768240102	39.0	36.0	41.0	31.5	41.0
30-31	37.18212122065749	39.0	36.0	40.0	31.0	41.0
32-33	37.397545491906754	39.0	36.0	41.0	32.0	41.0
34-35	37.399652669510715	39.0	36.0	41.0	31.5	41.0
36-37	37.28776454642437	39.0	36.0	41.0	31.0	41.0
38-39	37.17294037498543	39.0	36.0	41.0	31.0	41.0
40-41	37.15985637884316	39.0	36.0	40.5	31.0	41.0
42-43	37.09141533938544	39.0	36.0	40.0	31.0	41.0
44-45	37.062735637324444	39.0	35.5	40.0	31.0	41.0
46-47	37.07613377819999	39.0	36.0	40.5	31.0	41.0
48-49	36.99524463828435	39.0	35.0	40.0	31.0	41.0
50-51	36.91645923646114	39.0	35.0	40.0	30.5	41.0
52-53	36.79078697016867	39.0	35.0	40.0	30.0	41.0
54-55	36.690162805354106	39.0	35.0	40.0	30.0	41.0
56-57	36.51922234032999	38.0	35.0	40.0	30.0	41.0
58-59	36.38066346650568	38.0	35.0	40.0	30.0	41.0
60-61	36.222372318855705	38.0	34.5	40.0	29.5	41.0
62-63	35.99135092166949	38.0	34.0	40.0	29.0	41.0
64-65	35.78722348628915	37.0	34.0	40.0	29.0	41.0
66-67	35.53718275038137	37.0	34.0	40.0	29.0	41.0
68-69	35.26453280535872	36.5	34.0	39.0	28.5	41.0
70-71	34.98849985429071	36.0	33.5	39.0	28.0	40.0
72-73	34.68717357614706	35.5	33.0	39.0	28.0	40.0
74-75	34.36406228076522	35.0	33.0	38.0	28.0	40.0
76-77	33.29579791568452	34.5	31.5	36.5	26.5	39.0
78-79	33.66726650335133	35.0	32.0	37.0	28.0	39.0
80-81	33.59362045752357	35.0	32.0	36.0	28.0	39.0
82-83	33.3998923413116	35.0	32.0	36.0	28.0	37.5
84-85	33.16969374382307	35.0	32.0	35.5	28.0	37.0
86-87	32.99726210006551	34.5	32.0	35.0	27.0	37.0
88-89	32.804096615338565	34.0	32.0	35.0	27.0	36.0
90-91	32.70277984264399	34.0	32.0	35.0	27.0	36.0
92-93	32.6567330867386	34.0	32.0	35.0	27.0	36.0
94-95	32.64220458553129	34.0	32.0	35.0	27.5	35.5
96-97	32.551261367455545	34.0	32.0	35.0	27.0	35.0
98-99	32.68990192965648	34.0	32.0	35.0	28.5	35.0
100-101	32.252214466233355	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	18.0
20	83.0
21	387.0
22	1833.0
23	5953.0
24	15312.0
25	32389.0
26	56721.0
27	88488.0
28	123979.0
29	163759.0
30	205265.0
31	251669.0
32	307184.0
33	380210.0
34	482067.0
35	624603.0
36	865136.0
37	1239765.0
38	1279814.0
39	522666.0
40	16229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.80021898299866	22.833944946005353	16.2092590249824	24.15657704601359
2	13.51204074141108	23.180907220963086	40.90840055984322	22.39865147778261
3	16.104284650285262	28.419752230461597	32.06580715239413	23.410155966859012
4	11.71375956681225	20.271189554006728	39.957418972013485	28.057631907167536
5	12.247050400155715	38.84518583315663	36.47662177905378	12.431141987633884
6	27.964363038154993	40.259421018676136	19.39120565357916	12.385010289589708
7	24.818958597176184	33.43901303978326	24.73409368096284	17.007934682077718
8	23.79142529688839	37.513849639177785	21.780329377922904	16.914395686010916
9	25.21850652454382	16.71378132704718	21.687135544203215	36.380576604205785
10-11	23.370552339292786	27.398026661840397	29.893753026736125	19.337667972130692
12-13	24.471591597630514	24.917537426966625	29.775623914089905	20.835247061312955
14-15	21.599494322154424	26.11665646937037	27.334426747620743	24.94942246085446
16-17	21.133502642968118	29.23371257671046	27.89937497101762	21.733409809303804
18-19	21.24171390723448	28.51705023764165	29.450553721809385	20.790682133314483
20-21	22.125625803570127	27.680616901735238	29.190372001099167	21.003385293595468
22-23	21.795193157475097	27.623179017089498	29.068197701226715	21.513430124208686
24-25	21.575581344721964	27.827857764629965	29.076672074524545	21.519888816123522
26-27	21.65936096008445	27.882537215844614	28.938250995369042	21.519850828701898
28-29	21.60162910140179	27.971378010888227	28.95080008566308	21.4761928020469
30-31	21.825882971049353	27.82109110156502	28.875816947245575	21.47720898014005
32-33	21.657596871712283	27.982048771544594	28.859934765021094	21.50041959172203
34-35	21.68853603663021	28.012686595539027	28.90348921440575	21.395288153425017
36-37	21.707878433530535	27.973643943178615	28.851490901295428	21.46698672199542
38-39	21.61380290051767	28.007345263506288	28.868273927987797	21.51057790798825
40-41	21.790811324463647	27.923042350609727	28.73145359287562	21.554692732051002
42-43	21.684373342069478	28.03009939815306	28.56909074721832	21.71643651255915
44-45	21.81130611803645	28.10845783814574	28.383900136943375	21.69633590687444
46-47	21.860161771119664	28.114398867859595	28.147138768772834	21.87830059224791
48-49	21.79489664322277	28.20552209750661	28.136003160415747	21.863578098854873
50-51	21.82312511922599	28.287615643673792	28.0186490585163	21.870610178583924
52-53	21.95755157129123	28.175818290378007	27.946996214653698	21.919633923677065
54-55	21.894206660624892	28.10687134916409	28.01144084001146	21.987481150199557
56-57	21.948980988979677	28.203079690259457	27.95951273695481	21.888426583806055
58-59	21.8787075308173	28.266269312195064	27.976474527815398	21.878548629172233
60-61	21.978144396015992	28.235016586231033	27.933322734238512	21.85351628351446
62-63	21.966798885961754	28.239627556029482	27.89742279145403	21.89615076655473
64-65	21.899580802877257	28.196856970770106	27.936036628405652	21.967525597946988
66-67	21.940485079834747	28.233281110428766	27.83114148336311	21.99509232637338
68-69	21.92427591139201	28.216413439954362	27.87340041096611	21.98591023768752
70-71	21.921653100094243	27.98456329448606	27.95884259908208	22.13494100633762
72-73	21.951020964151812	28.06365449386041	27.872163509837712	22.11316103215006
74-75	21.81792448805578	28.154190314004175	27.923144146699215	22.104741051240836
76-77	21.929237463430837	28.065480118088924	27.893449356249267	22.111833062230968
78-79	21.92591705261901	28.08373371175345	27.8560019586921	22.134347276935443
80-81	21.91207348531562	28.07836684233358	27.876337830441884	22.133221841908917
82-83	21.927896626372664	28.007711733344127	27.95762236947518	22.106769270808023
84-85	22.014141179762973	28.02743496268436	27.899659120706232	22.058764736846438
86-87	21.869499934081237	28.042170908766238	27.964410627576473	22.123918529576052
88-89	21.899312000599764	28.02232782510126	27.935657259033746	22.142702915265225
90-91	21.910768780977634	28.00191249994089	28.051297845651675	22.0360208734298
92-93	21.942459808434208	27.92539168828952	28.006675939620468	22.1254725636558
94-95	21.746983975683225	27.935201979766877	28.10058614677499	22.217227897774904
96-97	21.726894667555317	28.00056183179197	28.184192697288335	22.08835080336438
98-99	21.83983881281605	28.643171202306206	28.259091644403277	21.257898340474465
100-101	22.388534240855478	31.353947811061424	25.50351445501923	20.754003493063873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1137.0
1	1131.0
2	1764.0
3	2522.5
4	3208.0
5	4721.5
6	6025.5
7	7275.5
8	8212.5
9	8206.5
10	7645.5
11	7022.5
12	7776.0
13	10387.0
14	16630.0
15	25526.0
16	32661.5
17	36138.5
18	36436.5
19	35248.5
20	33739.5
21	32445.5
22	32922.5
23	36967.0
24	44636.0
25	55914.0
26	69918.0
27	84626.5
28	100866.0
29	117666.0
30	131911.0
31	147033.5
32	164153.5
33	180339.5
34	195779.0
35	209712.5
36	224329.0
37	238289.0
38	248041.5
39	254922.5
40	262038.0
41	269781.5
42	273938.0
43	276656.5
44	279106.0
45	279793.5
46	278852.5
47	276354.5
48	273137.5
49	266511.0
50	258192.5
51	248543.0
52	235049.5
53	220609.0
54	205528.5
55	188834.5
56	171428.0
57	152769.5
58	133812.5
59	114949.0
60	96584.0
61	79205.5
62	62716.5
63	48190.5
64	35603.5
65	26322.5
66	19673.5
67	14567.5
68	11146.5
69	8194.0
70	5734.5
71	4166.5
72	2976.0
73	2017.0
74	1386.0
75	962.0
76	643.0
77	414.5
78	284.5
79	209.5
80	153.5
81	104.5
82	76.5
83	58.5
84	44.5
85	33.0
86	22.5
87	16.5
88	11.0
89	6.5
90	5.0
91	3.0
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003556672881089621
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.028776334949143E-4
14-15	0.0
16-17	0.0
18-19	4.7272234495494955E-4
20-21	0.0
22-23	0.0012683078169563149
24-25	1.9517494506200633E-4
26-27	0.004858886155546388
28-29	3.6068535627973496E-4
30-31	0.0
32-33	0.0
34-35	1.5118185279559056E-4
36-37	0.021045981485000812
38-39	0.010132846427282051
40-41	0.013602923959655065
42-43	0.001952576412954415
44-45	0.003040661698011798
46-47	0.0
48-49	7.417637898674109E-4
50-51	3.2209627989264854E-5
52-53	8.124629212234326E-6
54-55	0.004164305459019176
56-57	0.009287181504304881
58-59	0.011548306835084074
60-61	0.006051429544583442
62-63	0.006901189823204841
64-65	8.370065986321241E-4
66-67	1.3084786362512263E-4
68-69	0.0
70-71	1.250744304534797E-4
72-73	0.0
74-75	1.9224820451620324E-4
76-77	3.706958962852193E-5
78-79	0.0
80-81	0.002180395653262248
82-83	0.0021252508882824194
84-85	0.009112017261491969
86-87	0.010408712460163312
88-89	0.040691724985749314
90-91	4.0092022499643026E-4
92-93	6.185658665709392E-4
94-95	0.0
96-97	0.009726455286449616
98-99	5.76920924564243E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	168.0
20-21	683.0
22-23	1305.0
24-25	2691.0
26-27	3444.0
28-29	5829.0
30-31	13092.0
32-33	16478.0
34-35	23809.0
36-37	36963.0
38-39	45088.0
40-41	48228.0
42-43	53859.0
44-45	63723.0
46-47	64174.0
48-49	60383.0
50-51	56085.0
52-53	53908.0
54-55	57823.0
56-57	61112.0
58-59	62618.0
60-61	63041.0
62-63	59544.0
64-65	61421.0
66-67	65556.0
68-69	68405.0
70-71	69512.0
72-73	66539.0
74-75	66331.0
76-77	67501.0
78-79	73943.0
80-81	75998.0
82-83	72915.0
84-85	73084.0
86-87	77165.0
88-89	84427.0
90-91	93435.0
92-93	98903.0
94-95	121193.0
96-97	157114.0
98-99	549988.0
100-101	3866053.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.98709693451349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.89824837631546	79.06974742688037
2	6.501296857251739	11.310578798436097
3	1.4892065606364326	3.8862526633678445
4	0.5281769339327318	1.8377831260232267
5	0.23097474643941435	1.0045911328975006
6	0.11674675537412688	0.6093276795911465
7	0.0704843263850327	0.4291858852133108
8	0.044610731840829795	0.3104446443966279
9	0.02857982634790325	0.22374685124069604
>10	0.08999124178839854	1.1860530866174133
>50	0.001363422893705311	0.07504220555238428
>100	2.9442122208092267E-4	0.04598046576553789
>500	2.579957215628956E-5	0.011266034017821239
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5007058569998398E-5	0.0	0.0	0.0
3	0.0	3.0014117139996797E-5	0.0	0.0	0.0
4	0.0	3.0014117139996797E-5	0.0	3.0014117139996797E-5	0.0
5	0.0	4.5021175709995193E-5	0.0	3.0014117139996797E-5	0.0
6	1.5007058569998398E-5	4.5021175709995193E-5	0.0	3.0014117139996797E-5	1.5007058569998398E-5
7	1.5007058569998398E-5	4.5021175709995193E-5	0.0	3.0014117139996797E-5	1.5007058569998398E-5
8	3.0014117139996797E-5	4.5021175709995193E-5	0.0	3.0014117139996797E-5	1.5007058569998398E-5
9	3.0014117139996797E-5	1.50070585699984E-4	0.0	3.0014117139996797E-5	1.5007058569998398E-5
10-11	3.0014117139996797E-5	1.50070585699984E-4	0.0	3.0014117139996797E-5	1.5007058569998398E-5
12-13	6.0028234279993594E-5	1.50070585699984E-4	0.0	3.0014117139996797E-5	1.5007058569998398E-5
14-15	7.5035292849992E-5	2.2510587854997598E-4	0.0	3.0014117139996797E-5	1.5007058569998398E-5
16-17	9.004235141999039E-5	2.2510587854997598E-4	0.0	3.0014117139996797E-5	2.2510587854997597E-5
18-19	9.004235141999039E-5	2.401129371199744E-4	0.0	3.0014117139996797E-5	3.0014117139996797E-5
20-21	9.004235141999039E-5	2.551199956899728E-4	0.0	3.0014117139996797E-5	3.0014117139996797E-5
22-23	9.004235141999039E-5	3.00141171399968E-4	0.0	3.0014117139996797E-5	3.0014117139996797E-5
24-25	9.754588070498958E-5	3.7517646424995994E-4	0.0	6.0028234279993594E-5	3.0014117139996797E-5
26-27	1.050494099899888E-4	4.201976399599552E-4	0.0	1.1255293927498799E-4	3.0014117139996797E-5
28-29	1.050494099899888E-4	4.201976399599552E-4	0.0	3.676729349649608E-4	3.7517646424996E-5
30-31	1.050494099899888E-4	5.402541085199424E-4	0.0	0.0010129764534748918	4.5021175709995193E-5
32-33	1.050494099899888E-4	5.402541085199424E-4	0.0	0.002678759954744714	7.5035292849992E-5
34-35	1.200564685599872E-4	5.777717549449383E-4	0.0	0.004757237566689492	7.5035292849992E-5
36-37	1.425670564149848E-4	6.00282342799936E-4	0.0	0.007833684573539165	7.5035292849992E-5
38-39	1.50070585699984E-4	6.00282342799936E-4	0.0	0.013071148014468605	7.5035292849992E-5
40-41	1.50070585699984E-4	6.302964599399327E-4	0.0	0.01902895026675797	7.5035292849992E-5
42-43	1.50070585699984E-4	6.377999892249319E-4	0.0	0.024844185462632347	7.5035292849992E-5
44-45	1.50070585699984E-4	7.353458699299216E-4	0.0	0.031882495931961595	9.004235141999039E-5
46-47	1.50070585699984E-4	8.25388221349912E-4	0.0	0.03910089110413083	9.004235141999039E-5
48-49	1.50070585699984E-4	8.403952799199104E-4	0.0	0.045914095694910104	1.2005646855998719E-4
50-51	1.50070585699984E-4	9.679552777648967E-4	0.0	0.05330507204063431	1.2005646855998719E-4
52-53	1.5757411498498318E-4	9.754588070498959E-4	0.0	0.05957802252289364	1.350635271299856E-4
54-55	1.6507764426998238E-4	9.754588070498959E-4	0.0	0.06576093065373298	1.350635271299856E-4
56-57	1.6507764426998238E-4	9.904658656198944E-4	0.0	0.0722214693681173	1.350635271299856E-4
58-59	1.6507764426998238E-4	9.904658656198944E-4	0.0	0.07900465984175657	1.350635271299856E-4
60-61	1.6507764426998238E-4	0.001005472924189893	0.0	0.08518006444331092	1.350635271299856E-4
62-63	1.6507764426998238E-4	0.0010429905706148888	0.0	0.0919107302119552	1.50070585699984E-4
64-65	1.6507764426998238E-4	0.0012305788027398687	0.0	0.09852133951203948	1.50070585699984E-4
66-67	1.6507764426998238E-4	0.0012605929198798655	0.0	0.10499688528499379	1.50070585699984E-4
68-69	1.8008470283998077E-4	0.00131311762487486	0.0	0.11131485694296311	1.5757411498498318E-4
70-71	1.8008470283998077E-4	0.0013356282127298575	0.0	0.11748275801523246	1.6507764426998238E-4
72-73	1.8008470283998077E-4	0.0013356282127298575	0.0	0.12333551085753183	1.950917614099792E-4
74-75	1.8008470283998077E-4	0.001388152917724852	0.0	0.12802521666065633	1.950917614099792E-4
76-77	1.8008470283998077E-4	0.0015082093862848391	0.0	0.1320996330624109	1.950917614099792E-4
78-79	1.8008470283998077E-4	0.0015157129155698383	0.0	0.1357838659413455	1.950917614099792E-4
80-81	1.8008470283998077E-4	0.0015157129155698383	0.0	0.13820750590040026	1.950917614099792E-4
82-83	1.8008470283998077E-4	0.0015307199741398368	0.0	0.1397607364623951	1.950917614099792E-4
84-85	1.8008470283998077E-4	0.0015307199741398368	0.0	0.1413589882000999	1.950917614099792E-4
86-87	1.8008470283998077E-4	0.0015532305619948344	0.0	0.1416291152543599	1.950917614099792E-4
88-89	1.8008470283998077E-4	0.0015757411498498318	0.0	0.14165162584221488	2.100988199799776E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7510	0.0	50.36909	1
GTATCAA	13850	0.0	35.89845	1
ATCAACG	17655	0.0	27.587326	3
TCAACGC	17725	0.0	27.57354	4
CAACGCA	17955	0.0	27.196842	5
AACGCAG	18440	0.0	26.481525	6
TATCAAC	18665	0.0	26.208467	2
ACGCAGA	20880	0.0	23.346899	7
CGCAGAG	21110	0.0	23.092527	8
AGAGTAC	22485	0.0	19.448572	10-11
GTACATG	19080	0.0	19.317223	1
GCAGAGT	24775	0.0	19.301954	9
TACATGG	19440	0.0	18.634106	2
GAGTACT	13470	0.0	18.220373	12-13
ACATGGG	19775	0.0	18.040527	3
CAGAGTA	24715	0.0	17.1051	10-11
AGTACTT	15240	0.0	16.975845	12-13
GTACTTT	15540	0.0	16.024895	14-15
CATGGGG	13780	0.0	16.00471	4
ACTTTTT	18540	0.0	13.797111	16-17
>>END_MODULE