FastQCFastQC Report
Fri 27 May 2016
SRR2139672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5287920
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55100.049.5926361
GTATCAA101800.036.9240231
ATCAACG129150.027.8451123
TCAACGC129850.027.7613934
CAACGCA131900.027.29755
TATCAAC137550.026.8298422
AACGCAG135650.026.006976
ACGCAGA157150.022.3944847
CGCAGAG159800.021.7555148
GTACATG135550.019.8435361
TACATGG135500.019.1881082
AACGCCG3800.019.1302627
AGAGTAC169100.018.20708510-11
ACATGGG139350.018.1968023
GCAGAGT188550.018.1660969
GAGTACT112500.016.93333812-13
TATACCG5000.016.2494725
CATGGGG101950.016.2322734
GTGGTAT28050.016.1599831
AGTACTT127200.016.15301712-13
Produced by FastQC (version 0.11.5)