##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5287920
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.96773041195782	31.0	30.0	31.0	26.0	34.0
2	30.65127101015144	31.0	30.0	33.0	27.0	34.0
3	30.840889234330323	31.0	30.0	34.0	27.0	34.0
4	34.71829074569963	35.0	35.0	37.0	32.0	37.0
5	34.424100213316386	35.0	35.0	37.0	30.0	37.0
6	34.556132657075	35.0	35.0	37.0	30.0	37.0
7	34.37156519009365	35.0	35.0	37.0	30.0	37.0
8	34.36399813159049	35.0	35.0	37.0	30.0	37.0
9	35.71170781706228	37.0	35.0	39.0	30.0	39.0
10-11	35.707445271486705	37.0	35.0	39.0	30.0	39.0
12-13	35.58406774308234	37.0	35.0	39.0	30.0	39.0
14-15	36.45037008880618	38.0	35.0	40.0	30.0	41.0
16-17	36.28389517995734	38.0	35.0	40.0	29.5	41.0
18-19	36.51440434045901	38.0	35.0	40.0	30.0	41.0
20-21	36.4656733783081	38.0	35.0	40.0	30.0	41.0
22-23	36.28106256210267	38.0	34.0	40.0	29.5	41.0
24-25	36.33849860148463	38.0	35.0	40.0	30.0	41.0
26-27	36.10742902256689	38.0	34.0	40.0	29.0	41.0
28-29	35.797231552042135	38.0	34.0	40.0	27.5	41.0
30-31	35.84615092746637	38.0	34.0	40.0	27.0	41.0
32-33	35.91507455706626	38.0	34.0	40.0	28.5	41.0
34-35	35.600475451702614	38.0	34.0	40.0	27.0	41.0
36-37	35.47044316144491	38.0	34.0	40.0	26.5	41.0
38-39	35.59482845906015	38.0	34.0	40.0	27.0	41.0
40-41	35.568430992290544	38.0	34.0	40.0	27.0	41.0
42-43	35.55558161030988	38.0	34.0	40.0	27.0	41.0
44-45	35.707568137556194	38.0	34.0	40.0	27.5	41.0
46-47	35.44786370158617	38.0	33.5	40.0	26.5	41.0
48-49	35.286352499898605	38.0	33.0	40.0	26.5	41.0
50-51	35.21213442384321	38.0	33.0	40.0	26.5	41.0
52-53	35.315380694460245	38.0	33.0	40.0	27.0	41.0
54-55	35.33528261776041	38.0	33.0	40.0	27.0	41.0
56-57	35.25451986963708	37.5	33.0	40.0	27.5	41.0
58-59	34.81882390378068	37.0	33.0	40.0	26.0	41.0
60-61	34.32412418082838	36.5	32.0	40.0	25.5	41.0
62-63	34.214206806745835	36.0	32.0	39.0	25.5	41.0
64-65	34.16397925477834	36.0	32.0	39.0	25.5	40.5
66-67	33.844003627009734	36.0	31.5	39.0	25.5	40.0
68-69	33.575182353922784	35.0	31.0	39.0	25.5	40.0
70-71	33.668408979891375	35.0	31.0	38.0	26.0	40.0
72-73	33.483406065844775	35.0	31.0	38.0	26.0	40.0
74-75	33.00570040763037	35.0	31.0	37.0	25.5	39.5
76-77	32.599508417958724	34.0	31.0	36.5	24.5	39.0
78-79	32.54913834544192	34.0	31.0	36.0	25.5	39.0
80-81	32.40272054779577	34.0	31.0	36.0	25.5	38.5
82-83	32.47424787512414	34.0	31.0	35.5	26.0	37.0
84-85	32.094817417279	34.0	31.0	35.0	25.5	37.0
86-87	31.911182985366572	34.0	31.0	35.0	25.0	36.5
88-89	31.96189405757281	34.0	31.0	35.0	26.0	36.0
90-91	31.787989250803868	34.0	31.0	35.0	25.0	36.0
92-93	31.654192078223865	34.0	31.0	35.0	25.0	35.5
94-95	31.88408966884893	34.0	31.0	35.0	25.5	35.0
96-97	32.085266999528706	34.0	31.0	35.0	26.5	35.0
98-99	32.64393166261993	34.0	32.0	35.0	28.0	35.0
100	32.47364041327875	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	32.0
20	202.0
21	1008.0
22	4411.0
23	13466.0
24	30501.0
25	58271.0
26	94488.0
27	136853.0
28	181889.0
29	227381.0
30	272919.0
31	317834.0
32	366503.0
33	417858.0
34	478297.0
35	540280.0
36	613563.0
37	689868.0
38	612106.0
39	224738.0
40	5447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.83045507496331	21.812867819482896	14.986932480067777	26.369744625486014
2	14.381746866276826	23.267752721440946	38.589900220136016	23.760600192146214
3	16.82397177415161	27.48415775657683	30.99128409493413	24.700586374337426
4	12.535271490008057	20.97858681812156	38.19746588019352	28.28867581167686
5	12.823815947256378	39.191315146846996	35.22934069148941	12.755528214407216
6	28.01580511612532	39.720717531394264	19.325374042059735	12.938103310420681
7	24.630478524637287	33.84956655925203	24.13264572837713	17.387309187733553
8	23.78530688815262	36.48094146658799	21.544028653988715	18.18972299127067
9	24.43793106513167	17.550212096667895	22.21291589375707	35.798940944443366
10-11	23.29222117244452	27.371483759501896	29.59270915517942	19.743585912874163
12-13	24.23635380262939	24.770732159336752	29.396898969727225	21.59601506830663
14-15	21.45193195055901	26.25475612339067	27.045199246584666	25.24811267946565
16-17	20.866786940800917	28.998018124328656	27.846194723066915	22.289000211803504
18-19	21.084774353621082	28.35666008562913	29.41285987685139	21.145705683898395
20-21	21.769928934515335	27.472659498595785	29.242310305124374	21.515101261764507
22-23	21.406681606155182	27.428325830168816	29.28432333613433	21.880669227541677
24-25	21.327251674343966	27.57904420339985	29.1443011556685	21.949402966587684
26-27	21.50884927093461	27.661248689000406	28.98889499541884	21.84100704464614
28-29	21.39033627063901	27.539202511083605	29.147808582947455	21.922652635329932
30-31	21.780094735096938	27.598057316508285	28.953042705195397	21.66880524319938
32-33	21.611830178662764	27.75740211050518	28.93027794362623	21.700489767205823
34-35	21.593681695906827	27.64726132493603	28.961550411334418	21.797506567822726
36-37	21.66420375126554	27.63732606885697	28.90284078564945	21.795629394228044
38-39	21.61203351943173	27.711483700876947	28.867381850134777	21.809100929556546
40-41	21.817096523317552	27.599945343447246	28.76248906095868	21.820469072276527
42-43	21.687144076784552	27.770767954392067	28.613573134736864	21.928514834086517
44-45	21.839599366386338	27.879236038705002	28.32056560960653	21.96059898530213
46-47	21.783157170561545	27.78346041247806	28.185527232041522	22.247855184918876
48-49	21.771646299734197	27.93577898211293	28.155390180634036	22.137184537518834
50-51	21.774640464054112	28.033730137084607	28.077637805473316	22.11399159338797
52-53	21.936351234022002	27.965285824705948	27.96679685355441	22.13156608771764
54-55	21.902708906951762	27.892651583554418	28.07744311386656	22.127196395627262
56-57	21.912071855345832	27.96058432246072	28.093324545249864	22.034019276943585
58-59	21.795594540871388	27.943272277244528	28.115481582597035	22.145651599287046
60-61	21.842813514405584	27.868570690020373	28.11295640536412	22.175659390209926
62-63	21.798073074946338	28.056083982116625	28.08203252579904	22.063810417138
64-65	21.72697168057213	28.016371253734203	28.182326313087263	22.074330752606404
66-67	21.782189891803664	27.996176407042057	28.08930186393252	22.13233183722176
68-69	21.66060097711419	27.957018296555404	28.193835338291372	22.188545388039035
70-71	21.753911737886668	27.804911887012445	28.17760728235916	22.26356909274173
72-73	21.747178304906964	27.870699168515017	28.109305857544005	22.27281666903401
74-75	21.475020847355218	27.96817632885549	28.328585427933128	22.228217395856166
76-77	21.719417141746973	27.898327764860426	28.22789105339941	22.15436403999319
78-79	21.67550706383684	27.957938052224613	28.07249771128354	22.294057172655005
80-81	21.73071360303906	28.022944723195582	28.00580989247096	22.240531781294393
82-83	21.744018143608375	28.032571495063635	28.114647197299863	22.108763164028122
84-85	21.696409683991124	28.02277802846074	28.219552795875558	22.061259491672583
86-87	21.630297954694903	28.00304028639858	28.191945655472395	22.174716103434122
88-89	21.636573659196632	28.04282373427499	28.120412712339615	22.200189894188767
90-91	21.572832160547286	27.97803382767049	28.247870248515966	22.201263763266255
92-93	21.544954014029617	27.994674980514418	28.319875292283708	22.140495713172253
94-95	21.44148597129603	28.099743997384202	28.308139243217255	22.150630788102514
96-97	21.428854177698874	28.297551400046352	28.30860108614613	21.964993336108645
98-99	22.50949697163224	30.32299619431334	28.231406212860357	18.936100621194065
100	21.676969067908423	31.15672040634844	22.13857492791297	25.027735597830166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	385.0
1	386.5
2	1070.5
3	1838.5
4	2231.0
5	3514.0
6	4927.0
7	6150.0
8	7091.0
9	7125.5
10	6345.5
11	5242.0
12	5162.0
13	6563.5
14	10721.5
15	17072.5
16	22429.0
17	25557.0
18	26676.5
19	26579.5
20	26032.0
21	25377.0
22	25587.0
23	28061.0
24	33128.5
25	40800.5
26	50978.0
27	62059.5
28	73551.0
29	86335.0
30	99201.0
31	112126.5
32	125185.0
33	137109.5
34	148911.0
35	159851.5
36	171008.0
37	181805.0
38	189456.0
39	195161.5
40	200905.0
41	205850.5
42	209548.0
43	212046.5
44	212988.0
45	214441.0
46	214464.5
47	212767.5
48	210112.0
49	205205.5
50	199999.0
51	193637.5
52	184740.0
53	174406.0
54	162427.0
55	149691.0
56	137551.0
57	124786.5
58	110894.5
59	95823.0
60	79725.5
61	64635.0
62	51480.5
63	39972.5
64	30067.0
65	22054.5
66	16266.5
67	12617.5
68	9875.0
69	7437.0
70	5429.5
71	3940.0
72	2900.5
73	2098.0
74	1493.0
75	1039.0
76	694.0
77	482.5
78	334.5
79	228.5
80	167.5
81	125.0
82	93.0
83	64.0
84	41.0
85	31.5
86	27.0
87	20.5
88	12.5
89	6.0
90	5.0
91	5.0
92	4.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005295087671523018
3	1.8911027398296494E-5
4	1.7019924658466846E-4
5	1.8911027398296494E-5
6	2.2693232877955794E-4
7	0.0
8	0.0
9	1.8911027398296494E-5
10-11	5.295087671523019E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.039045648325141215
22-23	0.0
24-25	1.2301075057185806E-4
26-27	0.0013161902862694934
28-29	0.0
30-31	9.490021432264403E-6
32-33	0.0
34-35	9.533435569896384E-6
36-37	0.0
38-39	7.688554347074991E-5
40-41	0.002106590788168381
42-43	1.652289640931219E-4
44-45	8.807723864938839E-4
46-47	0.0021603300274036386
48-49	0.006420735277557155
50-51	0.005504419498415278
52-53	0.007685509359902834
54-55	0.009286876376366278
56-57	0.010516003979745973
58-59	0.012684898078438553
60-61	0.014457743355764614
62-63	0.01209461480219869
64-65	0.03615391454613564
66-67	0.004928491204024028
68-69	0.004020046201586986
70-71	0.003278928017790619
72-73	0.0011035355640558143
74-75	2.868261309637082E-4
76-77	7.797935440892485E-5
78-79	0.0
80-81	0.0
82-83	3.4597024240751E-5
84-85	1.168078698601471E-4
86-87	1.0651879648435895E-4
88-89	0.0
90-91	3.670243767803741E-5
92-93	0.0033545250360424387
94-95	1.2732646135444542E-5
96-97	1.0486049359931546E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	322.0
20-21	983.0
22-23	1859.0
24-25	3377.0
26-27	4135.0
28-29	6152.0
30-31	9868.0
32-33	12592.0
34-35	17129.0
36-37	22239.0
38-39	27820.0
40-41	29229.0
42-43	33464.0
44-45	39981.0
46-47	39173.0
48-49	35359.0
50-51	32595.0
52-53	31674.0
54-55	33649.0
56-57	36939.0
58-59	38752.0
60-61	38487.0
62-63	36209.0
64-65	38365.0
66-67	42705.0
68-69	43317.0
70-71	44522.0
72-73	43834.0
74-75	43589.0
76-77	45782.0
78-79	52255.0
80-81	52071.0
82-83	55706.0
84-85	55528.0
86-87	59004.0
88-89	74267.0
90-91	76149.0
92-93	79784.0
94-95	97389.0
96-97	223968.0
98-99	493639.0
100-101	3134059.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.37348399800614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.8444732757009	85.74896477158572
2	4.5666461940955205	8.345407458814853
3	0.9520527933006635	2.6097714202174567
4	0.31944581903742597	1.1675550973618483
5	0.12728825273111233	0.5815385562030226
6	0.06816938314796857	0.3737324424134913
7	0.03551924280063969	0.22718618745658814
8	0.0244825928655198	0.1789647845941818
9	0.015617876983816564	0.1284353849397232
>10	0.04596352408574359	0.6055643336389995
>50	2.3155743794766456E-4	0.014529665140451151
>100	1.0948781272512477E-4	0.018349897633681094
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8911027398296494E-5	0.0	0.0	0.0
3	0.0	1.8911027398296494E-5	0.0	1.8911027398296494E-5	0.0
4	1.8911027398296494E-5	7.564410959318598E-5	0.0	1.8911027398296494E-5	0.0
5	1.8911027398296494E-5	7.564410959318598E-5	0.0	1.8911027398296494E-5	0.0
6	1.7019924658466846E-4	7.564410959318598E-5	0.0	1.8911027398296494E-5	0.0
7	1.7019924658466846E-4	7.564410959318598E-5	0.0	5.6733082194889486E-5	0.0
8	1.7019924658466846E-4	7.564410959318598E-5	0.0	5.6733082194889486E-5	0.0
9	1.7019924658466846E-4	9.455513699148247E-5	0.0	7.564410959318598E-5	0.0
10-11	1.7019924658466846E-4	9.455513699148247E-5	0.0	1.0401065069063072E-4	0.0
12-13	1.796547602838167E-4	9.455513699148247E-5	0.0	1.1346616438977897E-4	0.0
14-15	1.8911027398296493E-4	9.455513699148247E-5	0.0	1.1346616438977897E-4	3.782205479659299E-5
16-17	3.214874657710404E-4	9.455513699148247E-5	0.0	1.3237719178807548E-4	9.455513699148247E-5
18-19	3.214874657710404E-4	1.1346616438977897E-4	0.0	1.607437328855202E-4	1.1346616438977897E-4
20-21	3.214874657710404E-4	1.1346616438977897E-4	0.0	1.8911027398296493E-4	1.2292167808892722E-4
22-23	3.214874657710404E-4	1.3237719178807548E-4	0.0	2.0802130138126144E-4	1.5128821918637195E-4
24-25	3.214874657710404E-4	1.607437328855202E-4	0.0	2.174768150804097E-4	1.5128821918637195E-4
26-27	3.214874657710404E-4	1.7019924658466846E-4	0.0	2.4584335617785445E-4	1.5128821918637195E-4
28-29	3.214874657710404E-4	1.796547602838167E-4	0.0	4.254981164616711E-4	1.5128821918637195E-4
30-31	3.214874657710404E-4	1.8911027398296493E-4	0.0	0.0010022844521097142	1.607437328855202E-4
32-33	3.214874657710404E-4	1.8911027398296493E-4	0.0	0.002222045719299838	1.7019924658466846E-4
34-35	3.214874657710404E-4	2.458433561778544E-4	0.0	0.004028048835837153	1.7019924658466846E-4
36-37	3.403984931693369E-4	3.025764383727439E-4	0.0	0.007110546301759482	1.7019924658466846E-4
38-39	3.593095205676334E-4	3.403984931693369E-4	0.0	0.012991875822629692	1.796547602838167E-4
40-41	3.593095205676334E-4	4.160426027625229E-4	0.0	0.018977215994190534	1.8911027398296493E-4
42-43	4.160426027625229E-4	4.254981164616711E-4	0.0	0.024924734110954777	1.8911027398296493E-4
44-45	4.160426027625229E-4	4.633201712582641E-4	0.0	0.03274444394015038	1.8911027398296493E-4
46-47	4.160426027625229E-4	5.105977397540053E-4	0.0	0.040819452639222986	1.8911027398296493E-4
48-49	4.160426027625229E-4	5.105977397540053E-4	0.0	0.047183013358749755	2.0802130138126144E-4
50-51	4.349536301608194E-4	5.200532534531536E-4	0.0	0.05371677332486119	2.0802130138126144E-4
52-53	4.349536301608194E-4	5.295087671523019E-4	0.0	0.06207544743490824	2.174768150804097E-4
54-55	4.349536301608194E-4	5.389642808514501E-4	0.0	0.0703773884627604	2.363878424787062E-4
56-57	4.349536301608194E-4	5.484197945505984E-4	0.0	0.07664639404529569	2.4584335617785445E-4
58-59	4.349536301608194E-4	5.484197945505984E-4	0.0	0.08268846729905142	2.552988698770027E-4
60-61	4.4440914385996766E-4	5.767863356480431E-4	0.0	0.08894801736788757	2.6475438357615095E-4
62-63	4.727756849574124E-4	5.862418493471914E-4	0.0	0.09651242832720616	2.6475438357615095E-4
64-65	4.727756849574124E-4	6.051528767454878E-4	0.0	0.10365134117006308	2.6475438357615095E-4
66-67	4.727756849574124E-4	6.051528767454878E-4	0.0	0.10891806230048867	2.6475438357615095E-4
68-69	4.727756849574124E-4	6.240639041437844E-4	0.0	0.11600969757484986	2.6475438357615095E-4
70-71	4.727756849574124E-4	6.429749315420808E-4	0.0	0.1208603761025129	2.742098972752992E-4
72-73	5.105977397540053E-4	6.52430445241229E-4	0.0	0.125266645486316	3.025764383727439E-4
74-75	5.105977397540053E-4	6.713414726395256E-4	0.0	0.12877464106869999	3.025764383727439E-4
76-77	5.484197945505984E-4	6.807969863386738E-4	0.0	0.1322448145962874	3.025764383727439E-4
78-79	5.673308219488948E-4	6.807969863386738E-4	0.0	0.13378606332924856	3.025764383727439E-4
80-81	5.673308219488948E-4	6.807969863386738E-4	0.0	0.1343061165827017	3.025764383727439E-4
82-83	5.673308219488948E-4	6.997080137369703E-4	0.0	0.13488290291834976	3.214874657710404E-4
84-85	5.673308219488948E-4	6.997080137369703E-4	0.0	0.13519493487042164	3.214874657710404E-4
86-87	5.767863356480431E-4	7.186190411352668E-4	0.0	0.13525166795261653	3.214874657710404E-4
88	5.862418493471914E-4	7.564410959318597E-4	0.0	0.13525166795261653	3.214874657710404E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5510	0.0	49.592636	1
GTATCAA	10180	0.0	36.924023	1
ATCAACG	12915	0.0	27.845112	3
TCAACGC	12985	0.0	27.761393	4
CAACGCA	13190	0.0	27.2975	5
TATCAAC	13755	0.0	26.829842	2
AACGCAG	13565	0.0	26.00697	6
ACGCAGA	15715	0.0	22.394484	7
CGCAGAG	15980	0.0	21.755514	8
GTACATG	13555	0.0	19.843536	1
TACATGG	13550	0.0	19.188108	2
AACGCCG	380	0.0	19.130262	7
AGAGTAC	16910	0.0	18.207085	10-11
ACATGGG	13935	0.0	18.196802	3
GCAGAGT	18855	0.0	18.166096	9
GAGTACT	11250	0.0	16.933338	12-13
TATACCG	500	0.0	16.249472	5
CATGGGG	10195	0.0	16.232273	4
GTGGTAT	2805	0.0	16.159983	1
AGTACTT	12720	0.0	16.153017	12-13
>>END_MODULE