FastQCFastQC Report
Fri 27 May 2016
SRR2139671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5231143
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.050.2365761
GTATCAA106400.037.2125631
TCAACGC137950.028.0823144
ATCAACG138300.028.0418623
CAACGCA141700.027.3391365
TATCAAC146000.026.8559972
AACGCAG144400.026.6208926
ACGCAGA166550.023.0548527
CGCAGAG166900.022.6738198
AACGCCG2703.767127E-918.9829987
GCAGAGT194250.018.9097049
AGAGTAC178150.018.60463510-11
GTACATG137500.018.1410161
TACATGG135000.018.002382
GAGTACT116750.017.68816412-13
ACATGGG137150.017.0969923
CAGAGTA191650.016.68123610-11
AGTACTT132600.016.63680612-13
GTGGTAT29700.016.3945641
TGGTATC27650.015.7563832
Produced by FastQC (version 0.11.5)