##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139671_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5231143
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.196769424961236	31.0	30.0	34.0	26.0	34.0
2	30.849243272454988	31.0	30.0	34.0	27.0	34.0
3	31.033886666833617	31.0	31.0	34.0	27.0	34.0
4	34.617341372621624	35.0	34.0	37.0	32.0	37.0
5	34.35106228218192	35.0	34.0	37.0	31.0	37.0
6	34.743728091547105	35.0	35.0	37.0	32.0	37.0
7	34.58013650171674	35.0	35.0	37.0	30.0	37.0
8	34.570424092784314	35.0	35.0	37.0	30.0	37.0
9	35.81567642100398	37.0	35.0	39.0	30.0	39.0
10-11	35.781620288338516	37.0	35.0	39.0	30.0	39.0
12-13	35.71664882799037	37.0	35.0	39.0	30.0	39.0
14-15	36.46839027340678	38.0	35.5	40.0	30.5	41.0
16-17	36.31278269395426	38.0	35.0	40.0	30.0	41.0
18-19	36.56120067832212	38.0	35.5	40.0	30.0	41.0
20-21	36.50152561779699	38.0	35.0	40.0	30.0	41.0
22-23	36.358752629407434	38.0	35.0	40.0	29.5	41.0
24-25	36.37952455054002	38.0	35.0	40.0	30.0	41.0
26-27	36.118156083360915	38.0	35.0	40.0	29.0	41.0
28-29	35.81655130446322	38.0	34.5	40.0	28.0	41.0
30-31	35.87155595261968	38.0	34.0	40.0	28.0	41.0
32-33	35.93073142423806	38.0	34.5	40.0	29.0	41.0
34-35	35.65354750926895	38.0	34.0	40.0	27.5	41.0
36-37	35.50621046362088	38.0	34.0	40.0	26.5	41.0
38-39	35.61221779479867	38.0	34.0	40.0	27.0	41.0
40-41	35.59420540568797	38.0	34.0	40.0	27.0	41.0
42-43	35.57577974038563	38.0	34.0	40.0	27.0	41.0
44-45	35.71353767570453	38.0	34.0	40.0	28.0	41.0
46-47	35.474520414376016	38.0	34.0	40.0	27.0	41.0
48-49	35.3222663317155	38.0	33.5	40.0	27.0	41.0
50-51	35.26278897962182	38.0	33.0	40.0	27.0	41.0
52-53	35.376277898577	38.0	33.5	40.0	27.0	41.0
54-55	35.39099276793306	38.0	34.0	40.0	27.5	41.0
56-57	35.30976851467625	38.0	33.5	40.0	27.5	41.0
58-59	34.91543698878637	37.0	33.0	40.0	26.0	41.0
60-61	34.45251987829677	37.0	32.5	39.0	26.0	41.0
62-63	34.397373198136464	37.0	32.0	39.0	25.5	40.5
64-65	34.347089439874395	36.5	32.5	39.0	26.0	40.0
66-67	34.0705114027169	36.0	32.0	39.0	26.0	40.0
68-69	33.83094636385259	36.0	32.0	38.0	26.0	40.0
70-71	33.9024817076708	35.5	32.0	38.0	26.0	40.0
72-73	33.708695753382784	35.0	32.0	38.0	26.0	40.0
74-75	33.27635196567603	35.0	31.0	38.0	26.0	39.0
76-77	32.92984135431952	35.0	31.0	37.0	25.5	39.0
78-79	32.847579679233725	34.5	31.0	37.0	25.5	39.0
80-81	32.71116554680541	34.0	31.0	36.0	25.5	38.0
82-83	32.7933562738446	34.0	31.0	36.0	26.0	38.0
84-85	32.428055427861366	34.0	31.0	36.0	26.0	38.0
86-87	32.28356170811563	34.0	31.0	35.0	25.5	37.0
88-89	32.3296779962088	34.0	31.0	35.0	26.0	37.0
90-91	32.150631546717335	34.0	31.0	35.0	25.5	37.0
92-93	32.041650466611	34.0	31.0	35.0	25.0	36.5
94-95	32.24994398300001	34.0	31.0	35.0	26.0	36.0
96-97	32.42865479710747	34.0	31.0	35.0	26.5	36.0
98-99	32.92422333157665	34.0	32.0	35.0	28.5	36.0
100	32.75245385942093	34.0	32.0	35.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	7.0
17	2.0
18	19.0
19	39.0
20	219.0
21	1182.0
22	4440.0
23	12992.0
24	29672.0
25	55948.0
26	89570.0
27	129378.0
28	171545.0
29	213033.0
30	254618.0
31	298130.0
32	342423.0
33	393975.0
34	455756.0
35	519968.0
36	609110.0
37	879148.0
38	558120.0
39	206955.0
40	4893.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.865633380697105	21.96806701709359	15.0348403780971	26.13145922411221
2	14.209816161092464	23.388035368400445	38.957545225625346	23.444603244881744
3	16.734857653425898	27.76692503604854	31.103921687448306	24.394295623077262
4	12.33350639976204	21.106540611095266	38.61005610257971	27.949896886562986
5	12.644722306174621	39.368194957159425	35.35339053311334	12.63369220355261
6	27.675511791907294	40.015809203747565	19.506531093664275	12.802147910680867
7	24.371901131358864	34.003486427344846	24.36131071163606	17.26330172966023
8	23.589376165017857	36.65189424185116	21.745878482006706	18.01285111112428
9	24.34396243572865	17.607114776680653	22.39897567280255	35.64994711478815
10-11	23.0830565342941	27.563353773019976	29.731385374133435	19.622204318552484
12-13	24.07327614634125	24.963167705413518	29.650843037554125	21.312713110691107
14-15	21.275130502071917	26.451312839278145	27.273532763298576	25.000023895351358
16-17	20.667261437892254	29.21828938723334	28.056191543607202	22.0582576312672
18-19	20.856055823369367	28.49945303535509	29.700385718798522	20.944105422477023
20-21	21.590347606318712	27.63807846050701	29.498460447721442	21.273113485452836
22-23	21.21321381273519	27.627506947988117	29.491825196105516	21.66745404317118
24-25	21.102075430513757	27.72579960480562	29.443349445536043	21.728775519144584
26-27	21.267904585469534	27.767543166793523	29.259278407268585	21.70527384046836
28-29	21.152404469001137	27.68649834554731	29.3762259619462	21.784871223505352
30-31	21.588945846165835	27.7104770883417	29.189477913735463	21.51109915175701
32-33	21.396706379393425	27.867239351514893	29.178281478122326	21.557772790969356
34-35	21.40555279760487	27.752630559505548	29.18260094236106	21.659215700528527
36-37	21.420719220262583	27.746894175867403	29.142045411666224	21.69034119220379
38-39	21.350209297099234	27.837544548049685	29.111951202632095	21.700294952218986
40-41	21.602957525882015	27.764795570552774	28.98984266763752	21.642404235927685
42-43	21.43328582882745	27.84333639925022	28.841196484306064	21.882181287616266
44-45	21.62217125062833	27.985692134763156	28.591592287621832	21.800544326986678
46-47	21.62197226740353	27.899241146964076	28.397189175940174	22.081597409692215
48-49	21.55364753524696	28.04192549831629	28.400991564541794	22.00343540189496
50-51	21.580828238625916	28.153679246887886	28.32219545368751	21.943297060798685
52-53	21.723438160537558	28.067054868262442	28.19472397504954	22.014782996150462
54-55	21.705104701193125	28.030721431719165	28.27742595171526	21.98674791537245
56-57	21.69946916316033	28.089148524817354	28.333834681533016	21.877547630489296
58-59	21.5845794945074	28.097947102477498	28.314599279866233	22.002874123148867
60-61	21.64422298501036	28.003887477604934	28.297022090592538	22.05486744679217
62-63	21.62731724730508	28.154426547973756	28.301488574777782	21.916767629943383
64-65	21.538163637948603	28.119388491731012	28.41311315102554	21.92933471929484
66-67	21.583474490250772	28.15212079093703	28.259392887409952	22.00501183140225
68-69	21.49966361184091	28.08232518952793	28.380083176267284	22.03792802236388
70-71	21.595037389159057	27.886306323062026	28.3980482147839	22.120608072995015
72-73	21.581768519040093	28.019094765838393	28.32093521055823	22.078201504563282
74-75	21.350567625055696	28.099057072934237	28.481183161385886	22.069192140624185
76-77	21.561006134800788	28.091710931528446	28.352577040787548	21.994705892883218
78-79	21.576810691975876	28.064639144904053	28.24453994860432	22.11401021451575
80-81	21.533079455118994	28.170791314892963	28.19763002243196	22.098499207556085
82-83	21.602880138672997	28.14728089842103	28.27056700779395	21.979271955112022
84-85	21.579378188390486	28.15498549187998	28.356594591433225	21.909041728296305
86-87	21.460808955564353	28.137412448143685	28.379956992175348	22.021821604116617
88-89	21.5220887193433	28.14737170145006	28.31716096550514	22.013378613701494
90-91	21.47117158950263	28.15725143515072	28.373186780559728	21.998390194786925
92-93	21.442387895981703	28.12731232999489	28.452333402686232	21.97796637133717
94-95	21.25692767859567	28.290298355696052	28.45365812677722	21.999115838931058
96-97	21.300329163923635	28.39235023041475	28.50663594470046	21.80068466096116
98-99	22.340383402123216	30.43877724563885	28.490819463391404	18.730019888846535
100	21.50985968578121	31.34280560951277	22.328513155691546	24.818821549014476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	384.0
1	385.0
2	1014.5
3	1755.0
4	2141.5
5	3386.0
6	4846.0
7	6155.5
8	7178.5
9	7209.5
10	6304.0
11	5185.5
12	5152.5
13	6541.0
14	10588.0
15	16867.0
16	22310.0
17	25703.5
18	26936.0
19	26767.0
20	26327.5
21	25973.0
22	26595.0
23	29244.0
24	34399.5
25	42732.5
26	53780.5
27	65402.0
28	77166.0
29	90299.5
30	103287.0
31	116004.5
32	129305.5
33	141987.0
34	153394.0
35	164125.0
36	175604.0
37	186053.5
38	193011.5
39	197728.0
40	202943.5
41	206625.5
42	208552.5
43	210443.0
44	211358.5
45	212113.0
46	210484.5
47	206366.0
48	202161.5
49	197180.0
50	191420.0
51	184953.5
52	176843.0
53	166332.0
54	154864.0
55	143103.0
56	130766.0
57	117850.5
58	104182.0
59	90294.5
60	76026.5
61	61669.0
62	48433.0
63	37154.5
64	28011.0
65	20984.0
66	15716.0
67	11918.5
68	9176.0
69	6931.5
70	5125.0
71	3758.0
72	2755.0
73	2039.5
74	1472.0
75	1045.5
76	734.0
77	507.0
78	358.0
79	248.0
80	176.5
81	128.5
82	101.5
83	78.5
84	54.0
85	34.5
86	26.0
87	18.0
88	10.5
89	7.5
90	6.0
91	5.0
92	5.0
93	4.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004951116801815588
3	3.823256217618214E-5
4	2.1027909196900181E-4
5	7.646512435236428E-5
6	2.4851165414518396E-4
7	0.0
8	0.0
9	3.823256217618214E-5
10-11	0.001175651286917601
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.558140544045535E-6
20-21	0.03760564639125836
22-23	0.0
24-25	1.0521671151289867E-4
26-27	0.004872930907105184
28-29	0.0
30-31	2.8789449703104004E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.720271957656941E-5
40-41	0.0018178777723491214
42-43	1.6714216975312706E-4
44-45	9.306679136296613E-4
46-47	0.002055820927219149
48-49	0.007128926075047582
50-51	0.005700589833853627
52-53	0.007928369929180702
54-55	0.009436454912618427
56-57	0.01061808843008464
58-59	0.01243716499663468
60-61	0.014225061453104712
62-63	0.011957669216614076
64-65	0.03423945373689441
66-67	0.005123277110110825
68-69	0.004021104839199161
70-71	0.0029647876221976606
72-73	9.498694260888928E-4
74-75	2.3415900399910127E-4
76-77	5.6293906432321886E-5
78-79	0.0
80-81	0.0
82-83	3.493842800832094E-5
84-85	8.253962639027684E-5
86-87	7.165975090353988E-5
88-89	2.423310110752542E-5
90-91	7.397996425288127E-5
92-93	0.0036663192452505156
94-95	3.8419498663961936E-5
96-97	1.0533234461372522E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	333.0
20-21	1030.0
22-23	1782.0
24-25	4047.0
26-27	4830.0
28-29	6476.0
30-31	10022.0
32-33	12633.0
34-35	17215.0
36-37	22275.0
38-39	27430.0
40-41	29593.0
42-43	33783.0
44-45	39619.0
46-47	38478.0
48-49	35231.0
50-51	32163.0
52-53	31309.0
54-55	33276.0
56-57	36440.0
58-59	37455.0
60-61	37684.0
62-63	35466.0
64-65	37223.0
66-67	41549.0
68-69	42802.0
70-71	43343.0
72-73	42971.0
74-75	42948.0
76-77	44635.0
78-79	50290.0
80-81	50280.0
82-83	53259.0
84-85	53598.0
86-87	56910.0
88-89	70658.0
90-91	71541.0
92-93	75382.0
94-95	92469.0
96-97	214159.0
98-99	477178.0
100-101	3141378.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.03158075100752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.64710588012424	85.24844081024682
2	4.667566069667068	8.49791835163121
3	1.0267270746817503	2.8039376582441244
4	0.32275338503280254	1.1752300332909835
5	0.13819872031667588	0.6290223984096692
6	0.07101255129982602	0.3878630878791106
7	0.039905965987853155	0.254289221568915
8	0.024611622448752743	0.17923479170855558
9	0.014362262347760477	0.11766775002095814
>10	0.04716975556526262	0.6529082154394921
>50	4.4770561965201786E-4	0.02617697924020175
>100	1.3900690834669463E-4	0.027310702319913734
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9116281088091074E-5	0.0	0.0	0.0
3	0.0	1.9116281088091074E-5	0.0	0.0	0.0
4	0.0	9.558140544045537E-5	0.0	0.0	0.0
5	1.9116281088091074E-5	9.558140544045537E-5	0.0	0.0	0.0
6	1.9116281088091074E-5	9.558140544045537E-5	0.0	0.0	0.0
7	1.9116281088091074E-5	9.558140544045537E-5	0.0	0.0	0.0
8	1.9116281088091074E-5	9.558140544045537E-5	0.0	0.0	0.0
9	3.823256217618215E-5	1.3381396761663752E-4	0.0	1.9116281088091074E-5	0.0
10-11	3.823256217618215E-5	1.3381396761663752E-4	0.0	2.867442163213661E-5	0.0
12-13	3.823256217618215E-5	1.3381396761663752E-4	0.0	3.823256217618215E-5	0.0
14-15	4.7790702720227685E-5	2.3895351360113843E-4	0.0	4.7790702720227685E-5	0.0
16-17	5.734884326427322E-5	2.4851165414518396E-4	0.0	7.64651243523643E-5	0.0
18-19	5.734884326427322E-5	2.6762793523327504E-4	0.0	9.558140544045537E-5	0.0
20-21	5.734884326427322E-5	2.9630235686541165E-4	0.0	1.0513954598450091E-4	0.0
22-23	5.734884326427322E-5	4.110000433939581E-4	0.0	1.2425582707259198E-4	0.0
24-25	6.690698380831876E-5	4.683488866582313E-4	0.0	1.3381396761663752E-4	9.558140544045537E-6
26-27	7.64651243523643E-5	4.7790702720227685E-4	0.0	1.816046703368652E-4	1.9116281088091074E-5
28-29	7.64651243523643E-5	4.7790702720227685E-4	0.0	3.632093406737304E-4	1.9116281088091074E-5
30-31	7.64651243523643E-5	4.7790702720227685E-4	0.0	0.001032279178756918	1.9116281088091074E-5
32-33	8.602326489640983E-5	4.7790702720227685E-4	0.0	0.0029056747253898433	1.9116281088091074E-5
34-35	1.0513954598450091E-4	5.830465731867778E-4	0.0	0.005209186596504817	1.9116281088091074E-5
36-37	1.2425582707259198E-4	6.690698380831876E-4	0.0	0.009118466079019442	1.9116281088091074E-5
38-39	1.3381396761663752E-4	7.264186813474608E-4	0.0	0.01591430400583582	1.9116281088091074E-5
40-41	1.3381396761663752E-4	8.028838056998251E-4	0.0	0.024201211857523296	1.9116281088091074E-5
42-43	1.3381396761663752E-4	8.411163678760073E-4	0.0	0.03181904987112759	1.9116281088091074E-5
44-45	1.3381396761663752E-4	8.984652111402805E-4	0.0	0.04063165545273757	1.9116281088091074E-5
46-47	1.3381396761663752E-4	9.175814922283716E-4	0.0	0.05046698207256044	2.867442163213661E-5
48-49	1.3381396761663752E-4	9.175814922283716E-4	0.0	0.0592126806703621	3.823256217618215E-5
50-51	1.3381396761663752E-4	9.844884760366903E-4	0.0	0.06812086765741254	3.823256217618215E-5
52-53	1.3381396761663752E-4	0.0010131628976688268	0.0	0.07787017101233898	4.7790702720227685E-5
54-55	1.3381396761663752E-4	0.0010131628976688268	0.0	0.08837456747024502	6.690698380831876E-5
56-57	1.3381396761663752E-4	0.0010131628976688268	0.0	0.09611666131092192	7.64651243523643E-5
58-59	1.3381396761663752E-4	0.0010131628976688268	0.0	0.10339040626494056	7.64651243523643E-5
60-61	1.6248838924877413E-4	0.001032279178756918	0.0	0.11144791874357096	8.602326489640983E-5
62-63	1.7204652979281967E-4	0.001051395459845009	0.0	0.12011715221702025	9.558140544045537E-5
64-65	1.7204652979281967E-4	0.0011469768652854644	0.0	0.12754382741974363	9.558140544045537E-5
66-67	1.7204652979281967E-4	0.0011852094274616466	0.0	0.13280080471896868	1.0513954598450091E-4
68-69	1.7204652979281967E-4	0.0012043257085497376	0.0	0.13908050305640662	1.1469768652854644E-4
70-71	1.7204652979281967E-4	0.0012330001301818741	0.0	0.14426101523127927	1.1469768652854644E-4
72-73	1.7204652979281967E-4	0.0012521164112699653	0.0	0.14910699248711037	1.1469768652854644E-4
74-75	1.7204652979281967E-4	0.0012903489734461474	0.0	0.15326478362377016	1.1469768652854644E-4
76-77	1.7204652979281967E-4	0.001338139676166375	0.0	0.1566292490952742	1.1469768652854644E-4
78-79	1.7204652979281967E-4	0.0013668140977985117	0.0	0.1583497143932024	1.1469768652854644E-4
80-81	1.7204652979281967E-4	0.0013859303788866027	0.0	0.15896143538802132	1.1469768652854644E-4
82-83	1.7204652979281967E-4	0.0013954885194306483	0.0	0.1597069703504569	1.1469768652854644E-4
84-85	1.7204652979281967E-4	0.0014050466599746939	0.0	0.16009885411276276	1.1469768652854644E-4
86-87	1.7204652979281967E-4	0.0014719536437830126	0.0	0.1601944355182032	1.1469768652854644E-4
88	1.7204652979281967E-4	0.0015101862059591947	0.0	0.1601944355182032	1.1469768652854644E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5790	0.0	50.236576	1
GTATCAA	10640	0.0	37.212563	1
TCAACGC	13795	0.0	28.082314	4
ATCAACG	13830	0.0	28.041862	3
CAACGCA	14170	0.0	27.339136	5
TATCAAC	14600	0.0	26.855997	2
AACGCAG	14440	0.0	26.620892	6
ACGCAGA	16655	0.0	23.054852	7
CGCAGAG	16690	0.0	22.673819	8
AACGCCG	270	3.767127E-9	18.982998	7
GCAGAGT	19425	0.0	18.909704	9
AGAGTAC	17815	0.0	18.604635	10-11
GTACATG	13750	0.0	18.141016	1
TACATGG	13500	0.0	18.00238	2
GAGTACT	11675	0.0	17.688164	12-13
ACATGGG	13715	0.0	17.096992	3
CAGAGTA	19165	0.0	16.681236	10-11
AGTACTT	13260	0.0	16.636806	12-13
GTGGTAT	2970	0.0	16.394564	1
TGGTATC	2765	0.0	15.756383	2
>>END_MODULE