FastQCFastQC Report
Fri 27 May 2016
SRR2139670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18857226
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA252950.050.389411
GTATCAA449200.035.6643681
TCAACGC565950.027.6044124
ATCAACG568250.027.5000423
CAACGCA579500.026.9445275
TATCAAC591050.026.6162132
AACGCAG592650.026.3890066
ACGCAGA681050.022.9392227
CGCAGAG688250.022.681028
GTACATG596000.020.570941
TACATGG610750.019.6079352
GCAGAGT803500.019.2513339
ACATGGG620600.018.8397463
GAGTACT457000.018.69457812-13
AGAGTAC731450.018.47772410-11
CAGAGTA781600.017.82448810-11
GTGGTAT115600.016.6620831
AGTACTT504900.016.45720312-13
CATGGGG439500.016.3936674
GTACTTT525300.016.27768914-15
Produced by FastQC (version 0.11.5)