##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18857226
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40819938203	31.0	31.0	34.0	30.0	34.0
2	31.54701041393893	31.0	31.0	34.0	30.0	34.0
3	31.57700422108745	31.0	31.0	34.0	30.0	34.0
4	35.39944236761017	37.0	35.0	37.0	33.0	37.0
5	35.22264234410724	37.0	35.0	37.0	32.0	37.0
6	35.24340409347589	37.0	35.0	37.0	32.0	37.0
7	35.14999130837165	37.0	35.0	37.0	32.0	37.0
8	35.144527726400476	37.0	35.0	37.0	32.0	37.0
9	36.66473912971081	39.0	35.0	39.0	32.0	39.0
10-11	36.54513609265753	38.5	35.0	39.0	32.0	39.0
12-13	36.486257734833316	38.0	35.0	39.0	32.0	39.0
14-15	37.53501668272948	39.0	36.0	41.0	32.0	41.0
16-17	37.51240919528673	39.0	36.0	41.0	32.0	41.0
18-19	37.526999490805274	39.0	36.0	41.0	32.0	41.0
20-21	37.5099107353585	39.0	36.0	41.0	32.0	41.0
22-23	37.41625644564022	39.0	36.0	41.0	32.0	41.0
24-25	37.299848799175464	39.0	36.0	41.0	31.0	41.0
26-27	37.079107517941296	39.0	36.0	40.5	31.0	41.0
28-29	36.89811781894512	39.0	36.0	40.0	30.5	41.0
30-31	36.726304154155876	38.5	35.5	40.0	30.0	41.0
32-33	36.53538071069865	38.0	35.0	40.0	30.0	41.0
34-35	36.390693374966126	38.0	35.0	40.0	30.0	41.0
36-37	36.23279010367662	38.0	35.0	40.0	30.0	41.0
38-39	36.06459905341647	38.0	34.5	40.0	29.5	41.0
40-41	35.857422465523186	38.0	34.0	40.0	29.0	41.0
42-43	35.65153002280171	38.0	34.0	40.0	28.0	41.0
44-45	35.84234457236815	38.0	34.0	40.0	29.0	41.0
46-47	35.81807035103391	38.0	34.0	40.0	28.5	41.0
48-49	36.1663738228152	38.0	34.5	40.0	29.0	41.0
50-51	36.23325396679969	38.0	35.0	40.0	29.0	41.0
52-53	36.15212844826172	38.0	34.0	40.0	29.0	41.0
54-55	36.019304201010186	38.0	34.0	40.0	28.5	41.0
56-57	35.898825325332524	38.0	34.0	40.0	28.0	41.0
58-59	35.72200457892928	38.0	34.0	40.0	28.0	41.0
60-61	35.51160493438242	37.5	34.0	40.0	28.0	41.0
62-63	35.28053207124327	37.0	33.0	40.0	28.0	41.0
64-65	35.04322763820113	37.0	33.0	39.5	28.0	41.0
66-67	34.76115360925937	36.0	33.0	39.0	27.0	41.0
68-69	34.48273768772269	36.0	32.0	39.0	26.5	40.5
70-71	34.22723865585236	35.5	32.0	39.0	26.0	40.0
72-73	33.90598702897104	35.0	32.0	38.0	26.0	40.0
74-75	33.59040801507449	35.0	31.5	37.5	26.0	39.5
76-77	31.748884673224424	33.5	29.5	35.5	25.0	38.5
78-79	32.87834141359702	34.0	31.0	36.0	26.0	39.0
80-81	32.9723440848005	34.0	31.0	36.0	26.0	38.5
82-83	32.7682078608576	34.0	31.0	36.0	26.0	37.5
84-85	32.54727259150539	34.0	31.0	35.0	26.0	37.0
86-87	32.36984382814272	34.0	31.0	35.0	26.0	36.5
88-89	32.21251646297001	34.0	31.0	35.0	26.0	36.0
90-91	32.04568095831952	34.0	31.0	35.0	26.0	36.0
92-93	32.02827118261284	34.0	31.0	35.0	26.0	35.5
94-95	32.046765321509625	34.0	31.0	35.0	26.0	35.0
96-97	32.16212742924181	34.0	31.0	35.0	26.5	35.0
98-99	32.32644707396656	34.0	31.0	35.0	27.0	35.0
100	30.80817722641384	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	2.0
18	6.0
19	47.0
20	329.0
21	2035.0
22	9253.0
23	29888.0
24	74202.0
25	147753.0
26	250002.0
27	370207.0
28	502792.0
29	631661.0
30	760290.0
31	895704.0
32	1050221.0
33	1247261.0
34	1514053.0
35	1883543.0
36	2437183.0
37	3101278.0
38	2826454.0
39	1094067.0
40	28993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.461742676255774	21.879331562341143	15.396198783426577	23.262726977976506
2	14.577148971817152	22.7939936433118	40.51758964102808	22.111267743842966
3	17.439928684951138	28.120964068309295	31.437219900971353	23.001887345768214
4	12.59701188287185	19.690913180973702	39.35072422635228	28.361350709802174
5	12.469113961936925	38.87290739369619	36.093437072875936	12.56454157149095
6	28.181983153612585	39.75000978405192	19.334479773209388	12.733527289126107
7	24.972198986213563	33.51172648617565	24.525325198944955	16.99074932866584
8	24.069388572847352	37.12264995922518	21.666749923875336	17.141211544052133
9	25.481912488309916	16.73699056493959	21.637187019321154	36.14390992742934
10-11	23.631566615040818	27.22627613553507	29.60081494789525	19.541342301528868
12-13	24.71204024671223	24.891560607444614	29.57597744202908	20.820421703814077
14-15	21.857791616605915	26.009402936444907	27.2002601886357	24.93254525831348
16-17	21.24520535377635	29.11198352651566	27.799802937587536	21.843008182120457
18-19	21.36069650784905	28.29196831398906	29.49589726055819	20.851437917603697
20-21	22.219534504283235	27.441435157937004	29.32942766254519	21.009602675234575
22-23	21.994079831333355	27.37915031159669	29.188706045927624	21.438063811142328
24-25	21.734533131541564	27.59815791516648	29.16108897723337	21.506219976058585
26-27	21.711937354601236	27.68557550038626	29.081233686923756	21.521253458088744
28-29	21.69690821875782	27.659117496211316	29.120075233253218	21.52389905177765
30-31	21.90434976924625	27.60366767254877	28.98405197889316	21.50793057931182
32-33	21.73203626996098	27.773324241948817	28.930915126070666	21.563724362019542
34-35	21.838039895115955	27.78374650560468	28.924695011478047	21.453518587801316
36-37	21.87914266971445	27.751238049730155	28.8312070787295	21.538412201825896
38-39	21.767846322734513	27.816410080834142	28.853345661877018	21.56239793455433
40-41	21.981227289923677	27.688203473570557	28.708825746339635	21.621743490166136
42-43	21.847971943209306	27.87295264909011	28.4848224625524	21.794252945148184
44-45	21.882424292637186	27.975551540243217	28.30574132190221	21.83628284521739
46-47	22.00333474837847	27.969913263484976	28.061298759731905	21.965453228404648
48-49	21.940776849618317	28.151907479841544	27.97402732281003	21.93328834773011
50-51	21.973439156058937	28.201314034136537	27.864382715597426	21.9608640942071
52-53	22.079256024768995	28.088904318539846	27.795710948967006	22.036128707724156
54-55	22.03187035231269	28.034661607384116	27.86558186721005	22.067886173093147
56-57	21.999524496397594	28.105602588908784	27.88186695325055	22.01300596144307
58-59	21.989744909286006	28.15770590163373	27.83092653084351	22.02162265823676
60-61	22.104275568034577	28.13813168353507	27.815179170240533	21.942413578189818
62-63	22.040442260324	28.20157415507389	27.797223749742795	21.960759834859317
64-65	22.033609924132932	28.15410977973316	27.768123280192942	22.044157015940964
66-67	22.07884518920818	28.126857984697008	27.70478525080986	22.08951157528495
68-69	21.994969714650356	28.11578030491166	27.773493947632407	22.115756032805585
70-71	22.033369185912576	27.95526324227896	27.83619817394618	22.175169397862284
72-73	22.055903259405817	28.035425364716026	27.730269655253558	22.178401720624603
74-75	21.92189102947965	28.120979205989048	27.801413665562478	22.155716098968817
76-77	22.054382484983257	28.029829963255796	27.8076301269028	22.108157424858142
78-79	22.004539288271495	28.01549144155024	27.765191343060597	22.21477792711767
80-81	22.002442279688104	28.069430331751338	27.789222647819862	22.138904740740692
82-83	21.987997947669918	28.00679534538692	27.897711955486688	22.10749475145647
84-85	21.992252682993236	27.99373847923495	27.92195822581765	22.09205061195416
86-87	21.927365786769382	27.995577293139984	27.996586421738236	22.080470498352398
88-89	21.955383016581592	27.987836016553473	27.92029032101782	22.13649064584711
90-91	21.992031368484263	27.933976377227115	28.0291245960345	22.044867658254123
92-93	21.965435251786104	27.84437235205931	28.098057791721594	22.092134604432996
94-95	21.777273977550646	27.86443639359431	28.210136759174876	22.148152869680164
96-97	21.799568533682425	28.036425126949567	28.287827567409057	21.87617877195895
98-99	22.84084786780594	29.991313779944434	28.562511541293965	18.60532681095566
100	22.238906594753615	30.58944300226294	22.412875307688722	24.758775095294723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3962.0
1	3715.0
2	5938.0
3	8748.0
4	11127.5
5	17531.5
6	23366.0
7	28427.5
8	32504.0
9	32213.0
10	28720.0
11	24615.5
12	25497.5
13	33253.0
14	52309.5
15	81404.0
16	105892.5
17	118567.0
18	121791.0
19	118521.5
20	111343.0
21	104678.5
22	101685.0
23	107354.0
24	125609.0
25	154612.0
26	190580.5
27	229180.0
28	271328.5
29	317716.5
30	360706.5
31	402896.5
32	448613.0
33	490381.5
34	529608.5
35	567309.5
36	606629.5
37	644975.0
38	673926.0
39	692649.0
40	709653.0
41	725885.5
42	738534.0
43	749592.0
44	756920.0
45	764979.5
46	765705.5
47	759784.0
48	753808.5
49	740733.5
50	724276.0
51	704541.5
52	676560.0
53	641397.5
54	602916.0
55	561222.0
56	517714.5
57	469734.5
58	417320.5
59	363562.0
60	307197.5
61	253235.5
62	204296.0
63	160213.5
64	121127.0
65	90784.5
66	68633.0
67	53034.0
68	41185.5
69	30260.5
70	22224.0
71	16636.5
72	12154.0
73	8787.0
74	6432.0
75	4686.5
76	3406.5
77	2380.5
78	1673.0
79	1211.5
80	859.0
81	609.0
82	452.0
83	322.0
84	246.5
85	190.5
86	149.5
87	118.5
88	88.5
89	62.5
90	40.5
91	31.0
92	26.0
93	13.0
94	2.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.13139790550317423
3	2.969683876090789E-4
4	0.0
5	0.0
6	4.2424055372725555E-5
7	0.0
8	0.0
9	0.002285595983205589
10-11	3.6855898105055324E-4
12-13	1.7765073187328825E-4
14-15	7.318149551795158E-4
16-17	0.0013575697719272178
18-19	4.5075558833520906E-5
20-21	0.002776327888195075
22-23	4.243504487572301E-5
24-25	8.304446638504598E-4
26-27	0.00518226809449834
28-29	0.001299325511889745
30-31	0.006075214508559586
32-33	0.005119177329263032
34-35	3.479006191373226E-5
36-37	9.139468995776114E-5
38-39	0.0
40-41	0.0
42-43	1.646138586871233E-5
44-45	0.0
46-47	0.0
48-49	2.2872068838489143E-4
50-51	0.0
52-53	1.1489892657401332E-5
54-55	3.47531118660388E-5
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.188730808728916E-4
64-65	0.0
66-67	0.0
68-69	3.103849785589161E-6
70-71	3.140646899141957E-6
72-73	3.1791357138065728E-6
74-75	3.217760389858701E-5
76-77	6.937182929214745E-4
78-79	0.003713405859741267
80-81	5.008774538195161E-5
82-83	6.775154964731931E-6
84-85	3.437751482263901E-6
86-87	3.491794335688048E-6
88-89	0.0
90-91	7.99778068856602E-5
92-93	5.176087132835748E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	836.0
20-21	2878.0
22-23	5581.0
24-25	11133.0
26-27	14454.0
28-29	22634.0
30-31	44022.0
32-33	54299.0
34-35	77734.0
36-37	101076.0
38-39	127343.0
40-41	134886.0
42-43	160307.0
44-45	177666.0
46-47	174714.0
48-49	159609.0
50-51	146265.0
52-53	140888.0
54-55	148707.0
56-57	159974.0
58-59	163616.0
60-61	167225.0
62-63	160729.0
64-65	167375.0
66-67	178518.0
68-69	185906.0
70-71	194070.0
72-73	188545.0
74-75	192824.0
76-77	170811.0
78-79	196152.0
80-81	213094.0
82-83	215341.0
84-85	219982.0
86-87	242365.0
88-89	301573.0
90-91	321644.0
92-93	353144.0
94-95	426567.0
96-97	856949.0
98-99	1400918.0
100-101	1.0474872E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.00286607492129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.19510973428446	72.32251772003517
2	7.717790620014303	12.657619012146332
3	2.1600649707966104	5.313945555400896
4	0.8568228755864483	2.810477260665779
5	0.4093312973214916	1.678316977726403
6	0.2226313151863129	1.0953843553984086
7	0.12322651714629693	0.7073449307698745
8	0.0840297270599097	0.5512542763524771
9	0.050657764183525335	0.37386736667969156
>10	0.17615569106957493	2.163325770830283
>50	0.0032486001094515857	0.17831583640235066
>100	8.684411146031947E-4	0.11223441849862902
>500	4.997764156779347E-5	0.02463010437057347
>1k	1.2468485427378837E-5	0.010766414723133525
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0606013843181389E-5
2	0.0	2.1212027686362778E-5	0.0	1.5909020764772083E-5	1.0606013843181389E-5
3	0.0	2.1212027686362778E-5	0.0	3.1818041529544166E-5	1.0606013843181389E-5
4	0.0	7.424209690226972E-5	0.0	4.772706229431625E-5	1.0606013843181389E-5
5	0.0	9.01511176670418E-5	0.0	4.772706229431625E-5	1.0606013843181389E-5
6	0.0	9.01511176670418E-5	0.0	5.3030069215906944E-5	2.6515034607953472E-5
7	0.0	9.54541245886325E-5	0.0	6.893908998067902E-5	3.712104845113486E-5
8	0.0	1.0606013843181389E-4	0.0	6.893908998067902E-5	4.2424055372725555E-5
9	0.0	2.3333230454999055E-4	0.0	7.424209690226972E-5	4.2424055372725555E-5
10-11	0.0	2.3333230454999055E-4	0.0	9.545412458863249E-5	4.5075558833520906E-5
12-13	0.0	2.3333230454999055E-4	0.0	1.1401464881419993E-4	5.3030069215906944E-5
14-15	0.0	3.2348342221703234E-4	0.0	1.3787817996135807E-4	8.219660728465576E-5
16-17	0.0	3.3408943606021374E-4	0.0	1.6969622149090222E-4	1.0075713151022319E-4
18-19	0.0	3.7916499489373465E-4	0.0	1.803022353340836E-4	1.1666615227499526E-4
20-21	0.0	4.0833153296248345E-4	0.0	1.8825674571646965E-4	1.2196915919658596E-4
22-23	0.0	4.8787663678634386E-4	0.0	2.0151426302044637E-4	1.3787817996135807E-4
24-25	0.0	5.409067060022509E-4	0.0	2.6780184954033003E-4	1.5378720072613014E-4
26-27	0.0	5.72724747531795E-4	0.0	3.7916499489373465E-4	1.5378720072613014E-4
28-29	0.0	5.72724747531795E-4	0.0	7.02648417110767E-4	1.5643870418692547E-4
30-31	0.0	6.41663837512474E-4	0.0	0.0017446892772033382	1.5909020764772082E-4
32-33	2.651503460795347E-6	6.41663837512474E-4	0.0	0.004133693895379946	1.749992284124929E-4
34-35	5.303006921590694E-6	7.371179621011065E-4	0.0	0.008376099432652502	1.749992284124929E-4
36-37	5.303006921590694E-6	8.299205832289437E-4	0.0	0.0139044841484108	1.803022353340836E-4
38-39	5.303006921590694E-6	8.458296039937157E-4	0.0	0.022816187280143962	1.856052422556743E-4
40-41	5.303006921590694E-6	8.909051628272366E-4	0.0	0.03150251261770952	1.90908249177265E-4
42-43	5.303006921590694E-6	9.333292181999621E-4	0.0	0.04158618027911422	1.9355975263806032E-4
44-45	5.303006921590694E-6	0.0010659043912397297	0.0	0.054583850243933016	1.9621125609885567E-4
46-47	5.303006921590694E-6	0.0011507525019851807	0.0	0.06797659422441031	2.0946877340283243E-4
48-49	5.303006921590694E-6	0.0011587070123675667	0.0	0.07856404754336613	2.3863531147158123E-4
50-51	5.303006921590694E-6	0.0012780246681033574	0.0	0.08769317395888451	2.3863531147158123E-4
52-53	5.303006921590694E-6	0.001283327675024948	0.0	0.09713252627931596	2.5454433223635333E-4
54-55	5.303006921590694E-6	0.0012886306819465388	0.0	0.11146125098145401	2.7310485646192073E-4
56-57	5.303006921590694E-6	0.0012992366957897202	0.0	0.12517747838414833	2.757563599227161E-4
58-59	5.303006921590694E-6	0.0013018881992505156	0.0	0.13696606277084444	2.810593668443068E-4
60-61	5.303006921590694E-6	0.0013177972200152876	0.0	0.14992130868029052	2.863623737658975E-4
62-63	5.303006921590694E-6	0.001344312254623241	0.0	0.16279170647899113	2.863623737658975E-4
64-65	5.303006921590694E-6	0.0014927964484277804	0.0	0.17279848054003277	2.8901387722669284E-4
66-67	5.303006921590694E-6	0.0015166599795749386	0.0	0.18318176809250736	2.916653806874882E-4
68-69	5.303006921590694E-6	0.0015564325314868688	0.0	0.19679458686023066	2.916653806874882E-4
70-71	5.303006921590694E-6	0.001580296062634027	0.0	0.20659984665825187	2.916653806874882E-4
72-73	5.303006921590694E-6	0.0015935535799380036	0.0	0.2135865582774476	3.1287740837385094E-4
74-75	5.303006921590694E-6	0.0016253716214675478	0.0	0.2190513069101468	3.1287740837385094E-4
76-77	5.303006921590694E-6	0.0017367347668209524	0.0	0.22292515346636882	3.155289118346463E-4
78-79	5.303006921590694E-6	0.0017818103256544732	0.0	0.22643839555192263	3.1818041529544164E-4
80-81	5.303006921590694E-6	0.0017818103256544732	0.0	0.22733725522513226	3.3674093952100904E-4
82-83	5.303006921590694E-6	0.0017844618291152686	0.0	0.2279338435038112	3.659074775897579E-4
84-85	5.303006921590694E-6	0.0017924163394976546	0.0	0.22825467542256744	3.712104845113486E-4
86-87	7.954510382386042E-6	0.001818931374105608	0.0	0.22842702314751917	3.712104845113486E-4
88	1.0606013843181389E-5	0.001924991512537422	0.0	0.2284535381821271	3.712104845113486E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25295	0.0	50.38941	1
GTATCAA	44920	0.0	35.664368	1
TCAACGC	56595	0.0	27.604412	4
ATCAACG	56825	0.0	27.500042	3
CAACGCA	57950	0.0	26.944527	5
TATCAAC	59105	0.0	26.616213	2
AACGCAG	59265	0.0	26.389006	6
ACGCAGA	68105	0.0	22.939222	7
CGCAGAG	68825	0.0	22.68102	8
GTACATG	59600	0.0	20.57094	1
TACATGG	61075	0.0	19.607935	2
GCAGAGT	80350	0.0	19.251333	9
ACATGGG	62060	0.0	18.839746	3
GAGTACT	45700	0.0	18.694578	12-13
AGAGTAC	73145	0.0	18.477724	10-11
CAGAGTA	78160	0.0	17.824488	10-11
GTGGTAT	11560	0.0	16.662083	1
AGTACTT	50490	0.0	16.457203	12-13
CATGGGG	43950	0.0	16.393667	4
GTACTTT	52530	0.0	16.277689	14-15
>>END_MODULE