FastQCFastQC Report
Fri 27 May 2016
SRR2139669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19839940
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA245250.045.9405821
GTATCAA424350.034.623171
ATCAACG523800.027.4929493
TCAACGC527050.027.339534
CAACGCA533400.026.990485
AACGCAG546500.026.4125426
TATCAAC556450.026.1623022
ACGCAGA627750.022.907197
CGCAGAG631600.022.754288
GCAGAGT743600.019.1073729
GTACATG547250.018.9160441
AGAGTAC676950.018.61332710-11
TACATGG547400.018.4507432
ACATGGG557300.017.7008043
GAGTACT448000.017.64005712-13
CAGAGTA735050.017.09360910-11
AGTACTT496200.016.34898812-13
CATGGGG369300.015.8024074
GTACTTT514850.015.40009614-15
GTGGTAT125950.015.09812451
Produced by FastQC (version 0.11.5)