FastQCFastQC Report
Fri 27 May 2016
SRR2139668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16276499
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA287400.052.3691671
GTATCAA560100.040.027481
TCAACGC697700.031.7185694
ATCAACG699400.031.6293433
CAACGCA704600.031.3898965
TATCAAC717100.030.9918522
AACGCAG727250.030.5580866
ACGCAGA826900.026.7557247
CGCAGAG845500.026.1721488
GCAGAGT953900.023.0690279
GTACATG702150.021.0729521
TACATGG701550.020.7039832
GAGTACT545950.020.4955412-13
ACATGGG693050.020.3939763
CAGAGTA943100.019.31252710-11
AGAGTAC891500.019.22897110-11
GTACTTT585600.019.14338114-15
AGTACTT589150.018.25112212-13
CATGGGG421650.017.8267884
ACTTTTT668350.016.98744616-17
Produced by FastQC (version 0.11.5)