##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16276499
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19745941679473	31.0	31.0	33.0	28.0	34.0
2	31.70017563359295	33.0	31.0	34.0	30.0	34.0
3	31.785198708886966	33.0	31.0	34.0	30.0	34.0
4	35.15852321804584	37.0	35.0	37.0	32.0	37.0
5	35.164581277583096	37.0	35.0	37.0	32.0	37.0
6	35.24512476546707	37.0	35.0	37.0	33.0	37.0
7	35.33515082082455	37.0	35.0	37.0	33.0	37.0
8	35.52819042964952	37.0	35.0	37.0	33.0	37.0
9	37.373558588981574	39.0	37.0	39.0	34.0	39.0
10-11	37.5096607691863	39.0	37.0	39.0	34.5	39.0
12-13	37.53113700925488	39.0	37.0	39.0	35.0	39.0
14-15	38.833348467628085	40.0	38.0	41.0	35.0	41.0
16-17	38.892982207045875	40.0	38.0	41.0	35.0	41.0
18-19	38.97098967658832	40.5	38.5	41.0	35.0	41.0
20-21	39.005770425007675	41.0	39.0	41.0	35.0	41.0
22-23	38.96898366093421	40.0	39.0	41.0	35.0	41.0
24-25	38.94448585473114	41.0	39.0	41.0	35.0	41.0
26-27	38.86096506160527	40.0	39.0	41.0	35.0	41.0
28-29	38.80098957360588	40.0	38.0	41.0	35.0	41.0
30-31	38.68780396525902	40.0	38.0	41.0	34.5	41.0
32-33	38.59269033797801	40.0	38.0	41.0	34.0	41.0
34-35	38.54164116519191	40.0	38.0	41.0	34.0	41.0
36-37	38.48041170993666	40.0	38.0	41.0	34.0	41.0
38-39	38.41418465307074	40.0	38.0	41.0	34.0	41.0
40-41	38.37888650639548	40.0	38.0	41.0	34.0	41.0
42-43	38.316711105812786	40.0	38.0	41.0	34.0	41.0
44-45	38.260160712468036	40.0	38.0	41.0	33.0	41.0
46-47	38.21405261964754	40.0	38.0	41.0	33.0	41.0
48-49	38.12753155876645	40.0	38.0	41.0	33.0	41.0
50-51	38.05385743595032	40.0	37.0	41.0	33.0	41.0
52-53	37.96596594418709	40.0	37.0	41.0	33.0	41.0
54-55	37.851634434344646	40.0	37.0	41.0	33.0	41.0
56-57	37.68910418533821	40.0	36.5	41.0	33.0	41.0
58-59	37.538040387327385	39.0	36.0	41.0	32.5	41.0
60-61	37.368750922646	39.0	36.0	41.0	32.0	41.0
62-63	37.18214982203726	39.0	35.0	41.0	32.0	41.0
64-65	36.95292546505592	38.5	35.0	40.0	32.0	41.0
66-67	36.698621454825066	38.0	35.0	40.0	31.0	41.0
68-69	36.4136337792645	37.5	35.0	40.0	31.0	41.0
70-71	36.122186849647235	37.0	35.0	39.5	31.0	41.0
72-73	35.7943781446646	36.5	34.5	39.0	31.0	41.0
74-75	35.4662994240051	36.0	34.0	39.0	31.0	40.0
76-77	35.442118780026135	36.0	35.0	38.5	31.0	40.0
78-79	35.18979020450942	35.0	34.0	37.0	31.0	39.0
80-81	34.91232117822217	35.0	34.0	37.0	31.0	39.0
82-83	34.62202982437738	35.0	34.0	36.5	31.0	39.0
84-85	34.372945426323234	35.0	34.0	36.0	31.0	37.0
86-87	34.17088958945729	35.0	34.0	36.0	31.0	37.0
88-89	33.99333646495057	35.0	34.0	35.5	30.0	37.0
90-91	33.86617593143704	35.0	34.0	35.0	30.0	36.0
92-93	33.77690700703351	35.0	34.0	35.0	30.0	36.0
94-95	33.72527193911466	35.0	34.0	35.0	30.0	36.0
96-97	33.71837839354697	35.0	34.0	35.0	31.0	36.0
98-99	33.75447967281005	35.0	34.0	35.0	31.0	35.5
100	33.27245883303815	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	10.0
17	258.0
18	2119.0
19	7616.0
20	15246.0
21	23770.0
22	33509.0
23	45386.0
24	60493.0
25	79572.0
26	103112.0
27	131296.0
28	163159.0
29	200362.0
30	246052.0
31	305222.0
32	387174.0
33	505897.0
34	713884.0
35	1054783.0
36	1719200.0
37	3227823.0
38	4606596.0
39	2535065.0
40	108895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.825364348930314	19.967918162253444	9.670716042805028	27.53600144601121
2	14.169350167276932	23.095791817205257	38.37375562208021	24.3611023934376
3	16.907690038256998	29.0953539824504	29.35760325362352	24.639352725669074
4	11.495752249915661	19.980672747867953	39.068051428012865	29.455523574203518
5	12.1765804796228	39.89717321888448	34.92983349797767	12.996412803515057
6	27.089350898633324	41.0307908870329	18.982505359358093	12.897352854975678
7	24.590793223846173	35.12818795698581	23.587948015819133	16.69307080334888
8	24.40738637958937	36.99584904591583	21.18613468412341	17.41062989037139
9	26.357338884731906	16.6443471658125	21.245496344146243	35.75281760530935
10-11	23.303570995212176	27.855913608940103	29.187944532789267	19.65257086305845
12-13	24.63777990586305	25.835764804212502	29.338330067172308	20.18812522275214
14-15	22.049938189956418	26.500322766327685	27.453962561072014	23.995776482643883
16-17	20.754930161043756	29.499136359374766	28.100271398313858	21.64566208126762
18-19	20.753467929436177	28.4761421974099	30.26644427649951	20.503945596654415
20-21	22.04954185425839	27.44385524289705	30.04483516667229	20.461767736172266
22-23	21.656205224996853	27.488856682595415	29.87442026211582	20.980517830291916
24-25	21.290719398284047	27.702890284093346	30.021915120768593	20.984475196854017
26-27	21.166849819331805	27.805565171754903	29.962582033709122	21.065002975204166
28-29	21.107177906471257	27.681254244196978	30.12551709182652	21.086050757505248
30-31	21.50104125627725	27.565230862216644	29.96125815834475	20.972469723161353
32-33	21.18735038125034	27.835773093896087	29.93133541775831	21.045541107095264
34-35	21.399410485072647	27.781739810817662	29.98500296867973	20.833846735429958
36-37	21.34911979593142	27.661273102653432	29.857887931555656	21.131719169859497
38-39	21.210363875221606	27.75815898023164	29.944164284894203	21.087312859652556
40-41	21.415596581785415	27.639897754055376	29.84319462155549	21.10131104260372
42-43	21.407580138870618	27.73398089482179	29.50452190995231	21.35391705635528
44-45	21.438338154173415	28.043384699437706	29.223816723567165	21.294460422821714
46-47	21.642519880004723	27.996884377882125	28.758315087996362	21.602280654116786
48-49	21.668382175208773	28.087137739170064	28.641521210297544	21.602958875323612
50-51	21.619420220872755	28.251226906060428	28.490044447039793	21.63930842602703
52-53	21.7129473042457	28.207738695400575	28.440968609956524	21.638345390397202
54-55	21.732497426261514	28.141655364147965	28.45853624638115	21.66731096320937
56-57	21.791815969300472	28.281707504172836	28.282571708728632	21.64390481779806
58-59	21.631755953347888	28.293520904902795	28.47849334915356	21.596229792595754
60-61	21.80372801803668	28.396576939473118	28.247126947694532	21.552568094795667
62-63	21.671830626517423	28.524266673985437	28.27213735478914	21.531765344708
64-65	21.677430404597185	28.326441754223787	28.27281620570588	21.72331163547315
66-67	21.79455522566857	28.452896488868106	28.101399587702264	21.651148697761062
68-69	21.745679515341287	28.325801712650982	28.24765289147256	21.680865880535176
70-71	21.7653342244192	28.056184995982274	28.324927041506022	21.853553738092504
72-73	21.821912405957345	28.144615537550127	28.188485481958068	21.84498657453446
74-75	21.660851380183356	28.29309117041015	28.22202222127433	21.824035228132168
76-77	21.764016062834813	28.24478928042602	28.191201001603183	21.79999365513598
78-79	21.691946962556834	28.226406122089383	28.183814690087498	21.89783222526628
80-81	21.737819575396124	28.30080076740758	28.124394002297787	21.836985654898513
82-83	21.890326396648973	28.1453162427406	28.24907504018365	21.715282320426777
84-85	21.861107560532066	28.16946704766713	28.161536249328346	21.807889142472458
86-87	21.671635947983606	28.325771063557887	28.265998709741336	21.736594278717174
88-89	21.814311438189932	28.187371799890393	28.184514209661003	21.81380255225867
90-91	21.848275443494202	28.20089527431429	28.217815721944316	21.733013560247194
92-93	21.87385679276529	28.238211132599716	28.19871040002389	21.6892216746111
94-95	21.636770031435095	28.244663277356153	28.32287437680111	21.795692314407642
96-97	21.746858841630033	28.408895913458075	28.325709351650097	21.51853589326179
98-99	22.612153314268603	30.302171738558727	28.647941419082084	18.43773352809059
100	21.82908918488939	31.203453986881165	22.458281255939323	24.509175572290122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10490.0
1	8959.0
2	5671.0
3	3828.5
4	3962.5
5	5584.5
6	8705.5
7	14647.5
8	24141.5
9	29878.5
10	27844.0
11	25467.0
12	28985.0
13	39319.5
14	62964.5
15	96133.5
16	124360.0
17	141009.5
18	142293.0
19	130136.5
20	111419.0
21	92527.0
22	80834.5
23	81909.0
24	94698.0
25	118689.5
26	153283.5
27	192493.5
28	230169.5
29	271682.0
30	314640.0
31	354206.0
32	399106.0
33	443001.5
34	483004.5
35	521708.5
36	562912.5
37	598948.0
38	619846.5
39	637887.5
40	653602.5
41	657133.5
42	652170.0
43	656284.5
44	667679.0
45	671834.5
46	662447.5
47	652358.0
48	644927.0
49	630550.0
50	613505.5
51	588755.5
52	550155.0
53	505869.0
54	464022.0
55	425473.5
56	389549.0
57	348792.0
58	308116.5
59	271586.0
60	230487.5
61	184052.5
62	140820.5
63	107564.5
64	81813.0
65	61004.0
66	45038.0
67	33616.0
68	25230.0
69	18521.5
70	13521.5
71	9683.0
72	6819.0
73	5083.5
74	3695.5
75	2578.0
76	1673.5
77	1013.5
78	685.0
79	458.0
80	295.0
81	193.5
82	147.0
83	108.0
84	69.5
85	45.0
86	35.0
87	28.0
88	19.5
89	13.0
90	11.0
91	11.5
92	9.5
93	6.5
94	5.5
95	5.0
96	5.0
97	3.5
98	2.5
99	6.0
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003041194546812555
3	0.0
4	0.0
5	0.0
6	0.0075507638344093535
7	5.5294446305682816E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1058889261136563E-4
16-17	3.256228504667988E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.804654453563569E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.103690160349359E-6
36-37	9.317572571035817E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.003860833937479279
52-53	0.0
54-55	0.0
56-57	9.421435412620406E-4
58-59	0.003508422639827382
60-61	0.003779781065570932
62-63	0.002494341440533597
64-65	0.0029442944736993108
66-67	0.0030498328928077988
68-69	0.0015311194509248975
70-71	0.0
72-73	0.003936240165068136
74-75	0.0030545508765811094
76-77	0.006337084656295045
78-79	2.6054351685286658E-5
80-81	1.9170117350437188E-4
82-83	0.003296232614983739
84-85	0.0021053755414726766
86-87	1.0069847951105466E-4
88-89	3.914505631212076E-5
90-91	4.0805408435991286E-4
92-93	0.0
94-95	4.3975226963068384E-4
96-97	5.521936328005343E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4149.0
20-21	10192.0
22-23	12324.0
24-25	12202.0
26-27	14439.0
28-29	21771.0
30-31	35677.0
32-33	42683.0
34-35	58457.0
36-37	87981.0
38-39	119080.0
40-41	122176.0
42-43	141355.0
44-45	171867.0
46-47	163595.0
48-49	139807.0
50-51	119915.0
52-53	106651.0
54-55	109863.0
56-57	116297.0
58-59	122956.0
60-61	122340.0
62-63	110702.0
64-65	115741.0
66-67	121238.0
68-69	127932.0
70-71	133534.0
72-73	115846.0
74-75	111440.0
76-77	119065.0
78-79	129284.0
80-81	136399.0
82-83	129870.0
84-85	127264.0
86-87	133693.0
88-89	147663.0
90-91	162199.0
92-93	172328.0
94-95	201840.0
96-97	519819.0
98-99	1002856.0
100-101	1.0602009E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.7548311692644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.58327600341244	69.85189390903244
2	7.9830127328580165	12.733676654623583
3	2.275779532526107	5.445132371852615
4	0.8985736182130147	2.8666234885493616
5	0.4474081485899557	1.784148067727254
6	0.25171952252097246	1.2045508812400807
7	0.15611857965584133	0.8715847673985888
8	0.09272954330393851	0.5916503256486887
9	0.06148168385608223	0.44131151843495336
>10	0.23763761299714334	3.007340117874351
>50	0.007810283821316351	0.42242058611205974
>100	0.004247042946704995	0.6301283646163933
>500	1.6829757080746735E-4	0.09588108318288553
>1k	3.7397727659925595E-5	0.053657863706808626
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.843148210189427E-5
2	0.0	7.986975577487517E-5	0.0	1.2287654734596181E-5	1.843148210189427E-5
3	0.0	7.986975577487517E-5	0.0	1.843148210189427E-5	1.843148210189427E-5
4	0.0	1.6588333891704845E-4	0.0	2.4575309469192362E-5	1.843148210189427E-5
5	6.143827367298091E-6	1.6588333891704845E-4	0.0	3.071913683649045E-5	1.843148210189427E-5
6	6.143827367298091E-6	1.7202716628434653E-4	0.0	3.071913683649045E-5	2.4575309469192362E-5
7	1.2287654734596181E-5	1.7202716628434653E-4	0.0	3.071913683649045E-5	2.4575309469192362E-5
8	1.2287654734596181E-5	1.904586483862408E-4	0.0	3.071913683649045E-5	3.071913683649045E-5
9	1.2287654734596181E-5	3.317666778340969E-4	0.0	4.9150618938384725E-5	3.071913683649045E-5
10-11	1.2287654734596181E-5	3.317666778340969E-4	0.0	6.451018735662996E-5	3.071913683649045E-5
12-13	1.2287654734596181E-5	3.3791050520139495E-4	0.0	7.065401472392804E-5	3.071913683649045E-5
14-15	1.2287654734596181E-5	4.362117430781644E-4	0.0	9.522932419312041E-5	3.686296420378854E-5
16-17	1.2287654734596181E-5	4.4235557044546253E-4	0.0	1.0137315156041849E-4	4.9150618938384725E-5
18-19	1.5359568418245225E-5	4.976500167511453E-4	0.0	1.0751697892771658E-4	4.9150618938384725E-5
20-21	2.7647223152841408E-5	5.560163767404772E-4	0.0	1.1673271997866372E-4	5.5294446305682816E-5
22-23	4.300679157108663E-5	6.451018735662995E-4	0.0	1.3823611576420703E-4	7.065401472392804E-5
24-25	4.300679157108663E-5	7.434031114430689E-4	0.0	1.9967438943718793E-4	8.601358314217327E-5
26-27	4.300679157108663E-5	7.833379893305065E-4	0.0	2.7032840416111594E-4	8.601358314217327E-5
28-29	4.9150618938384725E-5	7.864099030141556E-4	0.0	4.945781030674962E-4	8.601358314217327E-5
30-31	5.2222532622033774E-5	8.908549682582231E-4	0.0	0.0013854330713257194	8.601358314217327E-5
32-33	5.5294446305682816E-5	8.969987956255212E-4	0.0	0.003142567698372973	8.601358314217327E-5
34-35	5.5294446305682816E-5	0.001022947256655132	0.0	0.0052007498664178335	8.601358314217327E-5
36-37	5.5294446305682816E-5	0.0011488957176847428	0.0	0.008318742255321615	8.601358314217327E-5
38-39	5.5294446305682816E-5	0.0011796148545212333	0.0	0.013817467749053406	8.601358314217327E-5
40-41	5.5294446305682816E-5	0.0013762173302747723	0.0	0.02004423678581002	8.908549682582231E-5
42-43	5.5294446305682816E-5	0.001425367949213157	0.0	0.025628975862683985	9.215741050947135E-5
44-45	5.5294446305682816E-5	0.0015789636333956093	0.0	0.031834241503655054	9.215741050947135E-5
46-47	5.5294446305682816E-5	0.0017079840081088691	0.0	0.0381316645551356	9.522932419312041E-5
48-49	5.5294446305682816E-5	0.0017171997491598162	0.0	0.04426320426769909	1.1058889261136563E-4
50-51	5.5294446305682816E-5	0.0019230179659643024	0.0	0.05056369923286329	1.1058889261136563E-4
52-53	5.5294446305682816E-5	0.0019353056206988985	0.0	0.056956351608536945	1.228765473459618E-4
54-55	5.5294446305682816E-5	0.0019598809301680908	0.0	0.06290664841376514	1.4130802944785608E-4
56-57	5.5294446305682816E-5	0.0020028877217391776	0.0	0.06682333836041768	1.4745185681515417E-4
58-59	5.5294446305682816E-5	0.0020213192038410718	0.0	0.0700949264335039	1.4745185681515417E-4
60-61	5.5294446305682816E-5	0.002036678772259317	0.0	0.07329586049186622	1.4745185681515417E-4
62-63	5.5294446305682816E-5	0.00205818216804486	0.0	0.07636470226183162	1.4745185681515417E-4
64-65	5.5294446305682816E-5	0.002091973218565	0.0	0.07850275418565135	1.4745185681515417E-4
66-67	5.5294446305682816E-5	0.002098117045932298	0.0	0.08059165549053271	1.4745185681515417E-4
68-69	5.5294446305682816E-5	0.0021349800101360863	0.0	0.08280036142907636	1.4745185681515417E-4
70-71	5.5294446305682816E-5	0.002159555319605279	0.0	0.08458207136559281	1.5359568418245225E-4
72-73	5.5294446305682816E-5	0.002162627233288928	0.0	0.08612110012110097	1.5973951154975034E-4
74-75	5.5294446305682816E-5	0.002239425075380154	0.0	0.08740516004086628	1.5973951154975034E-4
76-77	5.5294446305682816E-5	0.002371517363777063	0.0	0.08851412088066359	1.5973951154975034E-4
78-79	5.5294446305682816E-5	0.002432955637450044	0.0	0.08930667461104505	1.5973951154975034E-4
80-81	5.5294446305682816E-5	0.002432955637450044	0.0	0.08957700301520616	1.628114252333994E-4
82-83	5.5294446305682816E-5	0.002436027551133693	0.0	0.08994870457092768	1.6588333891704845E-4
84-85	5.5294446305682816E-5	0.002451387119551938	0.0	0.0901115159961611	1.6588333891704845E-4
86-87	5.5294446305682816E-5	0.00250975347954127	0.0	0.09016988235615042	1.6588333891704845E-4
88	5.5294446305682816E-5	0.0027770099700187368	0.0	0.09017295426983407	1.6588333891704845E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28740	0.0	52.369167	1
GTATCAA	56010	0.0	40.02748	1
TCAACGC	69770	0.0	31.718569	4
ATCAACG	69940	0.0	31.629343	3
CAACGCA	70460	0.0	31.389896	5
TATCAAC	71710	0.0	30.991852	2
AACGCAG	72725	0.0	30.558086	6
ACGCAGA	82690	0.0	26.755724	7
CGCAGAG	84550	0.0	26.172148	8
GCAGAGT	95390	0.0	23.069027	9
GTACATG	70215	0.0	21.072952	1
TACATGG	70155	0.0	20.703983	2
GAGTACT	54595	0.0	20.49554	12-13
ACATGGG	69305	0.0	20.393976	3
CAGAGTA	94310	0.0	19.312527	10-11
AGAGTAC	89150	0.0	19.228971	10-11
GTACTTT	58560	0.0	19.143381	14-15
AGTACTT	58915	0.0	18.251122	12-13
CATGGGG	42165	0.0	17.826788	4
ACTTTTT	66835	0.0	16.987446	16-17
>>END_MODULE